Article
Chemistry, Applied
Haijuan Zeng, Jinbin Wang, Junwei Jia, Guogan Wu, Qianwen Yang, Xiaofeng Liu, Xueming Tang
Summary: This study developed a novel colloidal gold immunochromatographic strip for simultaneous detection of multiple transgenic proteins in crops, with high accuracy and ease of operation demonstrated in seed samples of maize, soybean, sugar beet, and cotton.
Article
Chemistry, Applied
Jun Li, Hongfei Gao, Yunjing Li, Fang Xiao, Shanshan Zhai, Gang Wu, Yuhua Wu
Summary: A specific event-specific PCR method was established for the identification and quantification of GM maize DBN9936, showing that duplex ddPCR outperformed qPCR in terms of precision across different platforms.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Chemistry, Multidisciplinary
Yujun Xing, Jie Liang, Fei Dong, Jirong Wu, Jianrong Shi, Jianhong Xu, Jinke Wang
Summary: We developed a novel loop-mediated isothermal amplification (LAMP) method using DNA captured on polyacrylamide microparticles (PAMMPs) as templates (PAMMPs@DNA-LAMP) for rapid qualitative detection of genetically modified organisms (GMOs). The PAMMPs@DNA-LAMP method allowed for rapid DNA extraction (5-10 min) and amplification (around 30 min) at a constant temperature of 63°C. The DNA captured by PAMMPs could be effectively detected by both conventional and quantitative PCR (qPCR) and LAMP.
Article
Plant Sciences
Zheng-Jun Guan, Min Zheng, Zhi-Xi Tang, Wei Wei, C. Neal Stewart Jr
Summary: In this study, differential expression of total protein in leaves of two transgenic oilseed rape lines and their non-transgenic parent plant was analyzed. Fourteen differential protein spots were identified, including eleven upregulated and three downregulated proteins, involved in photosynthesis, transporter function, metabolism, protein synthesis, and cell growth and differentiation. These changes in protein spots may be attributed to the insertion of foreign transgenes in transgenic oilseed rape, but transgenic manipulation does not necessarily cause significant changes in the proteomes of oilseed rape.
Article
Biochemistry & Molecular Biology
Yunjing Li, Fang Xiao, Chao Zhai, Xiaofei Li, Yuhua Wu, Hongfei Gao, Jun Li, Shanshan Zhai, Biao Liu, Gang Wu
Summary: In this study, the HPT gene sequence was cloned from soybean sprouts using a genome walking strategy, and qualitative PCR and quantitative real-time PCR methods were established. These assays were able to effectively differentiate between infected plants and GM plants, demonstrating broad applicability for routine GMO testing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Applied
Ji-Eun Park, Do-Geun Lee, Hong-Rae Kim, Mi-Ju Kim, Hae-Yeong Kim, Hyun-Joong Kim
Summary: In Korea, the development of rapid and accurate on-site detection methods for genetically modified canola events is crucial. This study developed ultrafast PCR assays for the event-specific detection of eleven GM canola events, showing high sensitivity and specificity.
Article
Biochemistry & Molecular Biology
Xiaoli Ma, Ensi Shao, Wenbo Chen, Rey O. Cotto-Rivera, Xiaowei Yang, Wendy Kain, Zhangjun Fei, Ping Wang
Summary: The study identified a 4 bp frameshift insertion in the ABCC2 gene as the specific mutation conferring Cry1Ac resistance in Trichoplusia ni through whole genome resequencing, and confirmed the importance of ABCC2 in the resistance process. Additionally, another gene mutation was found to be responsible for altered expression of APN1 and APN6. Furthermore, the study suggested the presence of additional Cry1Ac resistance-conferring mutation(s) in the resistant strain, which have yet to be identified.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Carlos Roman Collazo, Karen Chacha Guerrero, Tatiana Loja Mejia, Diego Andrade Campoverde, Yenima Hernandez Rodriguez
Summary: Partial approval of GMO use is research-oriented, with a rejection toward food. The linear regression model explained 65% of the variance of attitude toward GMOs from the beliefs, practices, knowledge, and bioethical approach variables. The sociodemographic variables were completely excluded from the model due to the absence of statistical significance.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Xinyu Zhou, Shengjie Ge, Yue Sun, Menglin Ran, Yifan Liu, Yu Mao, Xiaowei Cao
Summary: A novel biosensor was developed for the analysis of target DNA in maize, utilizing a high-density hot spot SERS substrate and HCR amplification strategy, exhibiting significant sensitivity and a broad detection range. Additionally, SERS was applied to detect target DNA in maize extract, confirming the feasibility of the detection strategy.
NEW JOURNAL OF CHEMISTRY
(2021)
Review
Medicine, General & Internal
Gabrielle M. O'Sullivan, Joshua G. Philips, Heidi J. Mitchell, Michael Dornbusch, John E. J. Rasko
Summary: Since 2001, gene technology in Australia has been regulated under a legislated national Gene Technology Regulatory Scheme, which aims to assess the potential risks of gene technology to human health and the environment. This scheme works alongside the Australian Therapeutic Goods Administration to ensure the safety and efficacy of therapeutic products. Recent reforms to the scheme aim to facilitate the safe development and delivery of gene-based human therapeutics in Australia, serving as a model for other jurisdictions.
FRONTIERS IN MEDICINE
(2022)
Review
Biotechnology & Applied Microbiology
Matan Arbel-Groissman, Itamar Menuhin-Gruman, Doron Naki, Shaked Bergman, Tamir Tuller
Summary: Evolutionary stability of synthetic constructs made of heterologous genes is a major challenge in synthetic biology, and the available tools to address this challenge are discussed in this review.
TRENDS IN BIOTECHNOLOGY
(2023)
Review
Pharmacology & Pharmacy
Rhys Whomsley, Victoria Palmi Reig, Ana Hidalgo-Simon
Summary: The European Union has implemented a streamlined procedure for assessing the environmental risks of gene therapy medicinal products, aiming to remove hurdles for developers and facilitate faster access to patients.
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Joshua G. Philips, Elena Martin-Avila, Andrea V. Robold
Summary: This review provides updated evidence on the likelihood, factors, and barriers for horizontal gene transfer in genetically modified plants, and presents the legislation and frameworks followed by the Australian Gene Technology Regulator in considering the risks posed by horizontal gene transfer.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biology
Pablo Rozas, Eduardo Kessi-Perez, Claudio Martinez
Summary: Genetic modification of living organisms has played a significant role in the research and development of various applications. However, regulatory frameworks have struggled to keep pace with new technologies and safety concerns. The emergence of new genome editing techniques has further challenged these frameworks. This review discusses the definitions of genetically modified organisms, the evolving genome editing tools, and how regulatory frameworks have responded to these technologies, with a focus on agricultural crops. It also explores the use of genetically modified organisms in the food industry, biomedical applications, and climate change solutions.
BIOLOGICAL RESEARCH
(2022)
Article
Food Science & Technology
Min Ki Shin, Seon Min Jeon, Yong Eui Koo
Summary: Although South Korea has a zero-tolerance policy towards unauthorized GMOs, efforts are being made to develop genetically modified potatoes with resistance to insects and viral diseases. A rapid detection method using ultra-fast PCR has been developed to assess GMOs in field conditions, reducing the runtime of the conventional methods by more than half.
FOOD SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Applied
Rajashekhar V. Ballari, Asha Martin
Article
Chemistry, Applied
Rajashekhar V. Ballari, Asha Martin, Lalitha R. Gowda
Article
Chemistry, Applied
Rohini Misra, Asha Martin, Lalitha R. Gowda
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2009)
Article
Environmental Sciences
Mohammad Hassan Kamani, Asha Martin, Manchanahally Shivanna Meera
WASTE AND BIOMASS VALORIZATION
(2020)
Article
Environmental Sciences
Cathrine M. S. Bethi, Bhaskar Narayan, Asha Martin, Tanaji G. Kudre
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Theresa Shalini, Asha Martin
JOURNAL OF FOOD BIOCHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
L. Sunil, Prakruthi Appaiah, Asha Martin, Prasanna Vasu
Summary: Through a combination of experimental data and theoretical modeling, a protein enriched with branched-chain amino acids was successfully designed and confirmed to be easily digestible and non-allergenic, offering a potential supplementary approach for the treatment of chronic liver diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Nutrition & Dietetics
Aisha A. Siddiq, Asha Martin
Summary: This study found that crocetin inhibits the expression of PCSK9, increases the expression of hepatic LDL receptor, and decreases the expression of SREBPs. It also regulates the expression of sortilin involved in PCSK9 transport. These findings suggest that crocetin has potential therapeutic effects in reducing cholesterol levels.
NUTRITION RESEARCH
(2022)
Review
Food Science & Technology
Arya K. P. Devi, Asha Martin
Summary: Lipid metabolism disorders are serious public health issues, and taurine has been found to regulate lipid metabolism through various physiological effects. However, the mechanisms underlying the lipid-lowering action of taurine remain unclear. This review focuses on the role of taurine in lipid metabolism and discusses taurine-related interventions for combating lipid disorders.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
C. Chinmayee, Asha Martin, B. S. Gnanesh Kumar, Sridevi Annapurna Singh
Summary: This study describes the production of a new thermostable fungal aspartic protease through solid-state fermentation from Rhizopus azygosporus, as well as its purification and characterization. The enzyme exhibited unusually high thermostability and specific enzyme activity, making it a potential candidate for various industrial applications.
PROCESS BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Prakruthi Appaiah, L. Sunil, Asha Martin, Prasanna Vasu
Summary: This study aimed to characterize the expressed and purified LNAA66 protein. The results showed that the protein had the expected structure and amino acid composition, and was completely digested under simulated digestion conditions. These findings suggest that the protein is suitable for dietary treatment of phenylketonuria.
Article
Biochemistry & Molecular Biology
K. P. Arya Devi, Aditya Rao Shimoga Janakirama, Asha Martin
Summary: Taurine activates SIRT1 and modulates lipid metabolic genes, leading to a decrease in lipid levels. Taurine may play a positive regulatory role in protecting against obesity and age-related diseases.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Article
Food Science & Technology
M. P. Thanushree, M. L. Sudha, Asha Martin, T. Vanitha, Crassina Kasar
Summary: This study investigated the impact of milling methods and improvers on the quality and nutritional properties of buckwheat noodles. The results showed that whole groat flour buckwheat noodles had a lighter color, finer texture, higher fiber content, and higher rutin content compared to fine flour buckwheat noodles. Adding gluten powder reduced cooking loss, while guar gum and xanthan gum increased water absorption capacity. The combination of emulsifiers had a better effect on overall quality. Whole groat flour buckwheat noodles had higher levels of dietary fiber, digestible starch, resistant starch, polyphenols, flavonoids, and minerals. The most abundant essential amino acids were leucine, phenylalanine, and lysine. This study confirmed the use of high buckwheat levels in producing buckwheat noodles with enhanced nutritional profile.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Janhavi Prakash, Sindhoora Sallaram, Asha Martin, Ravindra P. Veeranna, Muthukumar Serva Peddha
Summary: Different parts of Garcinia xanthochymus fruit have varying levels of phytochemicals and biological activity, with the peel containing the highest amounts of polyphenols and other beneficial compounds. The peel extract exhibits strong antioxidant and anti-diabetic potential.