Article
Chemistry, Multidisciplinary
Jialin Liu, Bo Cheng, Xinqi Fan, Xinyue Zhou, Jiankun Wang, Wen Zhou, Hengyu Li, Wenfeng Zeng, Pengyuan Yang, Xing Chen
Summary: Proteins are extensively modified with glycans of different structures, making it challenging to map the protein glycosylation landscape. This study presents Click-iG, a method that enables the simultaneous enrichment and profiling of three types of intact glycopeptides using metabolic labeling of glycans, optimized mass spectrometry, and tailored software analysis. Click-iG was able to identify thousands of intact glycosites in cell lines and living mice, providing comprehensive coverage of the protein glycosylation landscape and laying the foundation for investigating crosstalk between different glycosylation pathways.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Wei-Wei Shi, Chaowei Shi, Tong-Yue Wang, Yu-Lei Li, Yong-Kang Zhou, Xu-Han Zhang, Donald Bierer, Ji-Shen Zheng, Lei Liu
Summary: The chemical synthesis of correctly folded proteins with multiple or interchain disulfide bonds was expedited using a removable glycosylation modification (RGM) strategy, which introduces O-linked beta-N-acetylglucosamine (O-GlcNAc) groups to improve folding and can be efficiently removed afterwards.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Bing Xia, Ruqing Zhong, Qingshi Meng, Weida Wu, Liang Chen, Xin Zhao, Hongfu Zhang
Summary: This study investigated the mechanism of early-life stress (ELS)-induced intestinal diseases using a pig early weaning stress (EWS) model. The results showed that EWS disrupted ileal barrier integrity, leading to increased proportion of pathogens and unfolded protein response (UPR) process. Aberrant mucin O-glycans were also induced by EWS. The findings contribute to a comprehensive understanding of EWS and provide intervention targets for ELS-induced intestinal diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Agriculture, Multidisciplinary
Haiyan Wang, Ximei Zhang, Peng Kang, Xiuxiu Cui, Guo Hao, Zhongfu Wang, Bei Han, Xin Lv, Jing Zhang, Wupeng Ge
Summary: Oligosaccharides and N/O-glycans are abundant in human milk, with variations observed during lactation. Colostrum has the highest levels of oligosaccharides, which decrease as lactation progresses. Sialylated oligosaccharides and N/O-glycans are highly abundant in colostrum.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Tasnim Abukar, Sadia Rahmani, Nicole K. Thompson, Costin N. Antonescu, Warren W. Wakarchuk
Summary: The presence of glycans on proteins and cell surfaces can serve as biomarkers for various functional interactions and disease states. Sialic acid plays a key role in these interactions, and manipulating sialylation levels is crucial in therapeutic protein development. Different enzymes show varying abilities to transfer labeled sialic acids, with N-glycan type and target protein strongly influencing activity levels.
CARBOHYDRATE RESEARCH
(2021)
Article
Multidisciplinary Sciences
Chatchai Phoomak, Wei Cui, Thomas J. Hayman, Seok-Ho Yu, Peng Zhao, Lance Wells, Richard Steet, Joseph N. Contessa
Summary: This study demonstrates that cells lacking the SSR3 or SSR4 subunits can restore N-glycosylation over time and reduce the transcriptional signature of ER stress. The protein levels of the SSR3 subunit are dependent on ER stress and UBE2J1, coordinating upstream N-glycosylation proficiency with downstream ER-associated degradation and proteostasis. The TRAP complex plays a regulatory role in maintaining ER glycoprotein quality control under stress conditions in both non-transformed and tumor cells.
Article
Biochemistry & Molecular Biology
Thukaa Kouka, Sachiko Akase, Isami Sogabe, Chunsheng Jin, Niclas G. Karlsson, Kiyoko F. Aoki-Kinoshita
Summary: Research on glycan biosynthesis has made significant progress through simulation studies. Mathematical models for N-glycan and O-glycan biosynthesis have been developed, and the kinetics parameters for O-glycan biosynthesis have been optimized. The study suggests that additional factors are needed to accurately predict O-linked glycosylation.
Article
Biochemistry & Molecular Biology
Seonghun Kim, Jinhyuk Lee, Doo-Byoung Oh, Ohsuk Kwon
Summary: The invertebrate sialyltransferase cST3Gal-I from the sea squirt Ciona savignyi shows a preference for O-glycans as substrates, effectively transferring Neu5Ac to core 1 O-glycan compared to its human orthologue. Structural modeling suggests that the active sites of cST3Gal-I are similar to a mammalian enzyme, porcine ST3Gal-I, indicating a potential ancestral preference for O-glycans in C. savignyi.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Immunology
Benjamin Samuel Haslund-Gourley, Stephane Grauzam, Anand S. Mehta, Brian Wigdahl, Mary Ann Comunale
Summary: In this study, the researchers found that the glycosylation profile of IgG changes during acute Lyme disease infection, which is different from the classic proinflammatory IgG glycan signature. Additionally, IgG glycans detected during acute Lyme disease infection can effectively distinguish between control, acute, and treated cohorts.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Analytical
Siyuan Kong, Quanqing Zhang, Lujie Yang, Yuanyu Huang, Mingqi Liu, Guoquan Yan, Huanhuan Zhao, Mengxi Wu, Xiangmin Zhang, Pengyuan Yang, Weiqian Cao
Summary: A new enrichment strategy using a boronic acid-functionalized mesoporous graphene-silica composite is proposed for isolating intact glycopeptides, showing effectiveness and specificity in glycosylation analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Takashi Baba, Zoe Zhang, Suya Liu, Lyle Burton, Pavel Ryumin, J. C. Yves Le Blanc
Summary: We present a method to comprehensively characterize multiple glycosylations in glycopeptide isoforms. Using hot electron capture dissociation (hot ECD) in liquid chromatography-tandem mass spectrometry, we detected varying abundances of different O-glycoforms in isomeric glycopeptides. To capture low abundant glycosylated species, we utilized a prototype of a ZenoTOF 7600 system with an efficient electron-activated dissociation device in targeted or scheduled high-resolution multiple reaction monitoring workflows. Our method identified 69 O-glycopeptides with relative abundance ranging from 100 to 0.2%, including sialylated glycans with Neu5Ac and Neu5Gc.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Multidisciplinary Sciences
Asif Shajahan, Lauren E. Pepi, Bhoj Kumar, Nathan B. Murray, Parastoo Azadi
Summary: This study compares the glycosylation profiles of different variants of the SARS-CoV-2 virus and finds that the glycosylation sites on the spike protein remain highly conserved despite mutations. The newly emerged variants show changes in glycosylation patterns that may be related to their infectivity.
SCIENTIFIC REPORTS
(2023)
Article
Biochemical Research Methods
Moumen M. M. Alhasan, Sinisa Habazin, Fariz G. G. Kahhaleh, Gordan Lauc, Melanie L. L. Conrad, Mislav Novokmet
Summary: Researchers developed a method to analyze IgG-Fc glycosylation in lactating mice and found that antibiotic use during pregnancy affects the glycosylation patterns of IgG in offspring serum. This work contributes to a better understanding of mother-infant immunological interactions.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
John Benktander, Henrik Sundh, Kristina Sundell, Abarna V. M. Murugan, Vignesh Venkatakrishnan, Janos Tamas Padra, Jelena Kolarevic, Bendik Fyhn Terjesen, Marnix Gorissen, Sara K. Linden
Summary: The study revealed that stress can affect the skin barrier function of Atlantic salmon and lead to changes in mucin O-glycosylation. Both acute and chronic stress can increase the proportion of large O-glycan structures in fish.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Tana V. Palomino, David C. Muddiman
Summary: This study presents a new rule that can predict the number of sialic acids on a glycan. By utilizing infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) in negative-ion mode mass spectrometry, the number of sialic acids on the glycan can be predicted based on the experimental isotopic distribution of a detected glycan. This new rule improves the capability of IR-MALDESI to analyze sialylated N-linked glycans within biological tissues, providing confident glycan annotations and compositions.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Biochemistry & Molecular Biology
Toshimasa Harumoto, Hiroto Iwai, Mari Tanigawa, Toshiko Kubo, Toshiyuki Atsumi, Kyoko Tsutsumi, Michio Takashima, Giuseppe Destito, Rachel Soloff, Kazuma Tomizuka, Corwin Nycholat, James Paulson, Keiji Uehara
Summary: This study reports the uptake and enhancement of siRNA gene expression knockdown in CD22-expressing B cells using a chemically stabilized and modified CD22 glycan ligand-conjugated siRNA. This finding has the potential to broaden the use of siRNA technology and presents an innovative approach for targeted delivery of siRNAs to B cell lymphomas.
ACS CHEMICAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Zeshi Li, Luca Unione, Lin Liu, Yifei Lang, Robert P. de Vries, Raoul J. de Groot, Geert-Jan Boons
Summary: O-acetylation of sialic acids plays a role in various coronaviruses and influenza viruses, but different viruses exhibit distinct requirements for O-acetylation, and ester-to-amide perturbation can result in decreased or loss of binding. Molecular modeling studies suggest that binding specificity is governed by the complementarity between acetyl moieties of sialosides and hydrophobic patches of the lectin.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Review
Biochemistry & Molecular Biology
Zhengyuan Huang, Pei F. Lai, Alexander T. H. Cocker, Stuart M. Haslam, Anne Dell, Hugh J. M. Brady, Mark R. Johnson
Summary: Protein N-linked glycosylation is a diverse post-translational modification that stores biological information in a larger magnitude than other modifications. It impacts trophoblast functions and their interactions with decidual cells. Understanding N-glycosylation can improve approaches to predicting and diagnosing pregnancy complications related to placental dysfunction.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2023)
Article
Chemistry, Multidisciplinary
Angeles Canales, Javier Sastre, Jose M. Orduna, Cindy M. Spruit, Javier Perez-Castells, Gema Dominguez, Kim M. Bouwman, Roosmarijn van der Woude, Francisco Javier Canada, Corwin M. Nycholat, James C. Paulson, Geert-Jan Boons, Jesus Jimenez-Barbero, Robert P. de Vries
Summary: Influenza virus infection remains a threat to human health due to the drifting of viral hemagglutinins, which evade infection and vaccine-induced antibody responses. Recent H3N2 and pandemic H1 viruses specifically recognize glycan structures containing at least three N-acetyllactosamine units. This study characterizes the glycan specificity of H1 variants, including the one responsible for the 2009 pandemic outbreak, and investigates the preference for tri-LacNAc motifs in human-type receptor-adapted viruses using glycan arrays, tissue binding analyses, and nuclear magnetic resonance experiments.
Article
Chemistry, Multidisciplinary
Lifeng Sun, Pradeep Chopra, Ilhan Tomris, Roosmarijn van der Woude, Lin Liu, Robert P. de Vries, Geert-Jan Boons
Summary: The emergence of new SARS-CoV-2 variants and the dangers of long-covid necessitate the development of broadacting therapeutics that can reduce viral burden. SARS-CoV-2 employs heparan sulfate (HS) as an initial cellular attachment factor, and therefore, there is interest in developing heparin as a therapeutic for SARS-CoV-2. Its use is, however, complicated by structural heterogeneity and the risk of causing bleeding and thrombocytopenia.
Article
Chemistry, Multidisciplinary
Gael M. Vos, Yunfei Wu, Roosmarijn van der Woude, Robert P. de Vries, Geert-Jan Boons
Summary: This paper introduces a chemo-enzymatic strategy for synthesizing poly-N-acetyl lactosamines (polyLacNAc) in a regioselective manner. The strategy involves enzymatic assembly of oligo-LacNAc chains and modification at specific positions using traceless blocking groups. The methodology allows for the synthesis of polyLacNAc chains with various topologies.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Meeting Abstract
Immunology
Belen Gimeno-Molina, Erna Bayar, Katherine Mountain, Ryan Love, Yun Lee, Ingrid Muller, Anne Dell, Stuart Haslam, Paola Grassi, Gang Wu, David MacIntyre, Phillip Bennett, Pascale Kropf, Lynne Sykes
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2023)
Meeting Abstract
Immunology
Katherine Mountain, David MacIntyre, Denise Chan, Alice Hyde, James Pasint-Magyar, Yun Lee, Richard Brown, Anna David, Anne Dell, Ten Feizi, Stuart Haslam, Yan Liu, Holly Lewis, Jane Norman, Sarah Stock, Tiong Teoh, Vasso Terzidou, Samit Kundu, Phillip Bennett, Lynne Sykes
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Chika Kikuchi, Aristotelis Antonopoulos, Shengyang Wang, Tadashi Maemura, Rositsa Karamanska, Chiara Lee, Andrew J. Thompson, Anne Dell, Yoshihiro Kawaoka, Stuart M. Haslam, James C. Paulson
Summary: The evolution of human H3N2 influenza viruses has led to a limited specificity of the hemagglutinin to a subset of glycan receptors, presenting challenges for virus recognition and vaccine production. By studying glyco-engineered cell lines, the authors demonstrate the importance of extended glycan receptors for the growth of recent H3N2 viruses and their relevance to vaccine production.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Yiran Xie, Fangqi Zhao, Nancy Freitag, Sophia Borowski, Yiru Wang, Charlotte Harms, Poh-Choo Pang, Juliette Desforges, Tianyu Wen, Edzard Schwedhelm, Manvendra Singh, Ralf Dechend, Anne Dell, Stuart M. Haslam, Gabriela Dveksler, Mariana G. Garcia, Sandra M. Blois
Summary: Placental abnormalities can lead to impaired fetal growth and poor pregnancy outcomes, such as preeclampsia (PE), with long-term consequences for both mother and offspring. Galectin-1 (gal-1), a glycan-binding protein expressed at the maternal-fetal interface, plays a critical role in maternal adaptation to pregnancy and placenta development. Deficiency of gal-1 during pregnancy is associated with the development of PE, but the specific contributions of maternal- and placental-derived gal-1 to the disease onset are not well understood. This study demonstrates that the lack of gal-1 increases the risk of PE development in a niche-specific manner, and this is accompanied by placental dysfunction primarily influenced by the absence of maternal-derived gal-1. Notably, differential placental glycosylation through Sda-capped N-glycans plays a dominant role in the invasive capacity of trophoblasts triggered by maternal-derived gal-1. These findings highlight the essential role of maternal-derived gal-1 in healthy placenta development and suggest that impairment of the gal-1 signaling pathway within the maternal niche may be a molecular cause of maternal cardiovascular maladaptation during pregnancy.
Article
Chemistry, Multidisciplinary
Lifeng Sun, Pradeep Chopra, Ilhan Tomris, Roosmarijn van der Woude, Lin Liu, Robert P. de Vries, Geert-Jan Boons
Summary: The emergence of new SARS-CoV-2 variants and the risks of long-covid highlight the need for broad-acting therapeutics to reduce viral burden. Researchers have developed well-defined heparin mimetics that can inhibit viral binding and have lower side effects.
Article
Chemistry, Multidisciplinary
Pouya Zaree, Ilhan Tomris, Sander D. de Vos, Roosmarijn van der Woude, Frits M. Flesch, Robertus J. M. Klein Gebbink, Robert P. de Vries, Roland J. Pieters
Summary: A miniaturized sensor containing graphene- and gold nanoparticles was functionalized with proteins for electrochemical detection. The interactions between molecules and proteins were observed and quantified using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The protein binders included carbohydrate ligands as small as carbohydrates and COVID-19 spike protein variants engaged in protein-protein interactions.
Article
Chemistry, Multidisciplinary
Maria Pia Lenza, Unai Atxabal, Corwin Nycholat, Iker Oyenarte, Antonio Franconetti, Jon Imanol Quintana, Sandra Delgado, Reyes Nunez-Franco, Carmen Teresa Garnica Marroquin, Helena Coelho, Luca Unione, Gonzalo Jimenez-Oses, Filipa Marcelo, Mario Schubert, James C. Paulson, Jesus Jimenez-Barbero, June Ereno-Orbea
Summary: Siglec-8 is an inhibitory receptor that regulates inflammatory responses and can trigger cell apoptosis and inhibit mast cell degranulation. The structure of Siglec-8 and the antibody 2C4 that binds to it were determined using X-ray crystallography. The binding mode of a high-affinity analogue of its ligand was also deduced. The study provides insights into the molecular recognition features of Siglec-8 and potential strategies for designing Siglec-8 inhibitors.
Article
Biochemistry & Molecular Biology
Alexander Graewe, Cindy M. Spruit, Robert P. de Vries, Maarten Merkx
Summary: Fast and reliable virus diagnostics are crucial to prevent the spread of viruses. In this study, a new sensor platform called dark-LUX is introduced for detecting viral surface proteins. The platform utilizes a general bioluminescent framework that can be modified with different binding domains to enable detection of specific viral targets. The researchers used influenza A virus surface proteins as a proof of concept and optimized various parameters to improve the sensor's performance. The modular nature of the platform allows easy adaptation to detect newly emerging viral epitopes.
RSC CHEMICAL BIOLOGY
(2023)
Article
Veterinary Sciences
Nicolas Gaide, Marie-Noelle Lucas, Mattias Delpont, Guillaume Croville, Kim M. Bouwman, Andreas Papanikolaou, Roosmarijn van der Woude, Iwan A. Gagarinov, Geert-Jan Boons, Robert P. De Vries, Romain Volmer, Angelique Teillaud, Timothee Vergne, Celine Bleuart, Guillaume Le Loc'h, Maxence Delverdier, Jean-Luc Guerin
Summary: This study describes the pathobiological characteristics of the H5 highly pathogenic avian influenza outbreaks that occurred in Southwestern France in 2015-2016. The infection in chickens and guinea fowls was characterized by severe systemic vasculitis and parenchymal necrosis, while in ducks, the lesions were mild. Glycan-binding analyses revealed different affinities of the virus to the mucosae of different bird species, providing insights into its adaptability and transmission mechanisms.
VETERINARY RESEARCH
(2022)