Article
Biochemistry & Molecular Biology
Miriam Weckener, Laura S. Woodward, Bradley R. Clarke, Huanting Liu, Philip N. Ward, Audrey Le Bas, David Bhella, Chris Whitfield, James H. Naismith
Summary: The study reveals the regulatory mechanism of polysaccharide polymerization in Enterobacteriaceae bacteria. It demonstrates the formation of a complex between WzyE polymerase and WzzE polysaccharide co-polymerase, which together participate in the polymerization and regulation of polysaccharides. These findings provide important structural insights into the mechanism of polysaccharide chain length regulation in bacteria.
Article
Mathematics
Hongchuan Xia, Chunping Zhong
Summary: This paper presents a characterization for the general complex (alpha, beta) metrics to be strongly convex, and applies it to show that complex Randers metrics are strongly convex complex Finsler metrics, while complex Kropina metrics are only strongly pseudoconvex.
ACTA MATHEMATICA SCIENTIA
(2021)
Article
Chemistry, Physical
Yingang Wang, Jinhui Feng, Wenyue Dong, Xi Chen, Peiyuan Yao, Qiaqing Wu, Dunming Zhu
Summary: The study successfully engineered an omega-transaminase with (S)-selectivity to accept bulky substrates, resulting in several variants that showed activity towards bulky substrates, with the best variant achieving high yield and enantioselectivity in the asymmetric synthesis at a semi-preparative scale. This work will enhance the general understanding of omega-transaminases and inspire further engineering efforts to reverse enantio-specificity.
Article
Chemistry, Multidisciplinary
Shunsuke Kato, Akira Onoda, Ulrich Schwaneberg, Takashi Hayashi
Summary: Evolutionary engineering was performed on a previously reported artificial metalloenzyme through a DNA recombination strategy to enhance its catalytic activity towards C(sp(2))-H bond functionalization. Improved scaffold design was achieved by embedding alpha-helical cap domains of fatty acid binding protein (FABP) within the beta-barrel structure of nitrobindin (NB). Through directed evolution, an engineered variant, NBHLH1(Y119A/G149P), with enhanced performance and stability was obtained. Further rounds of metalloenzyme evolution resulted in a Cp*Rh(III)-linked NBHLH1(Y119A/G149P) variant with a significantly increased catalytic efficiency for the cycloaddition reaction. This DNA recombination strategy-based metalloenzyme engineering process is a powerful method for optimizing active-sites of artificial metalloenzymes.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Stefan Bucur, Marius Niculaua, Catalina Ionica Ciobanu, Neculai Catalin Lungu, Ionel Mangalagiu
Summary: This communication presents a novel synthesis route for preparing monofunctionalized beta-cyclodextrin in a single stage, which involves in-situ protection of secondary hydroxyl groups as a substitute for the conventional five-step procedure.
Article
Pharmacology & Pharmacy
Ying Qu, Xiao Sun, Long Ma, Chunyan Li, Zixuan Xu, Wenqing Ma, Yingying Zhou, Zhongxi Zhao, Dedong Ma
Summary: By embedding DSF in HP-β-CD to prepare an inclusion complex with enhanced solubility and anti-GBM activity, intranasal administration of DSF/HP-β-CD/Cu showed promising therapeutic effects in inhibiting tumor growth and migration, promoting apoptosis, and extending median survival time in rats compared to oral and intravenous administrations. Additionally, no obvious histopathological damage to normal tissues was observed, indicating the potential of DSF/HP-β-CD/Cu as an effective intranasal formulation for GBM treatment.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Chemistry, Organic
Zalina T. Gugkaeva, Zorayr Z. Mardiyan, Alexander F. Smol'yakov, Artavazd S. Poghosyan, Ashot S. Saghyan, Victor I. Maleev, Vladimir A. Larionov
Summary: A practical and efficient method for synthesizing artificial s-aryl-substituted cysteine derivatives was developed by using sequential Pd(II)-catalyzed Heck cross-coupling reaction and hydrothiolation reaction with various alkyl thiols in the presence of triethylamine. The reactions were performed in the ligand sphere of a robust and bench-stable chiral dehydroalanine Ni(II) complex, and enantiopure cysteine derivatives were obtained through subsequent acidic decomposition of the diastereomeric Ni(II) complexes.
Article
Chemistry, Multidisciplinary
Xiaoping Chen, Farong Ye, Xiaosheng Luo, Xueyi Liu, Jie Zhao, Siyao Wang, Qingqing Zhou, Gong Chen, Ping Wang
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Article
Chemistry, Multidisciplinary
Xiaoping Chen, Xiaosheng Luo, Xiao Peng, Jiaojiao Guo, Jiantao Zai, Ping Wang
CHEMISTRY-A EUROPEAN JOURNAL
(2020)
Article
Chemistry, Organic
Dan Lu, Hongli Yin, Siyao Wang, Feng Tang, Wei Huang, Ping Wang
JOURNAL OF ORGANIC CHEMISTRY
(2020)
Article
Chemistry, Organic
Dan Lu, Siyao Wang, Hongli Yin, Fengting Chu, Quhao Wei, Ping Wang
Article
Chemistry, Multidisciplinary
Hongli Yin, Mengjie Zheng, Huan Chen, Siyao Wang, Qingqing Zhou, Qiang Zhang, Ping Wang
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Chemistry, Organic
Xiaoping Chen, Xiaosheng Luo, Kaiqian Wang, Feng Liang, Ping Wang
Summary: The study introduces an efficient visible-light-promoted C-H alkylation method for nitrogen-containing heteroarenes using C4-alkyl DHPs as radical precursors at ambient temperature. A broad scope of heteroarenes can be successfully alkylated in good yields by utilizing various C4-alkyl DHPs.
Article
Chemistry, Organic
Xiaoping Chen, Xiaosheng Luo, Kaiqian Wang, Feng Liang, Ping Wang
Article
Chemistry, Multidisciplinary
Farong Ye, Jie Zhao, Peng Xu, Xinliang Liu, Jing Yu, Wei Shangguan, Jiazhi Liu, Xiaosheng Luo, Cheng Li, Tianlei Ying, Jing Wang, Biao Yu, Ping Wang
Summary: Investigating the role of carbohydrate domains in RBD glycoproteins is crucial for the development of neutralizing antibodies and vaccines against SARS-CoV-2, but the high heterogeneity of glycoforms may result in incomplete neutralization effects. This study presents an efficient and scalable strategy for preparing six glycosylated RBDs with defined structure glycoforms, providing insights into the structure-function relationships of carbohydrates.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Organic
Xiaosheng Luo, Ping Wang
Summary: In this study, the conversion of 4-aryl-1,4-dihydropyridines (DHPs) into ynones under electrochemical conditions is reported. The reaction proceeds via the homolysis of acyl-DHP under electron activation and resulting acyl radicals react with hypervalent iodine(III) reagents to form the target ynones or ynamides in acceptable yields. This methodology shows wider functionality tolerance and synthetic utility for late-stage modification of complex molecules.
Article
Chemistry, Multidisciplinary
Siyao Wang, QingQing Zhou, Xiaheng Zhang, Ping Wang
Summary: Site-selective peptide functionalization using photoredox catalysis provides a powerful approach for manipulating native peptides, resulting in itaconated peptides and their derivatives, which can be further functionalized as peptide-protein conjugates with excellent selectivity under mild conditions.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Siyao Wang, Qingqing Zhou, Yunxue Li, Bingcheng Wei, Xinliang Liu, Jie Zhao, Farong Ye, Zhongneng Zhou, Bei Ding, Ping Wang
Summary: Native chemical ligation (NCL) and expressed protein ligation (EPL) techniques provide efficient ways to assemble proteins with precise chemical features. This study introduces a quinoline-based photolabile protecting group (PPZQ) for cysteine, enabling various ligation strategies including NCL and EPL-desulfurization methods. The PPZQ group is compatible with radical desulfurization and shows great potential in protein caging/uncaging investigations.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Organic
Xiaosheng Luo, Qiping Feng, Ping Wang
Summary: The electro-induced homolysis of 4-alkyl-1,4-dihydropyridines (alkyl-DHPs) can generate C(sp(3))-centered radicals. The resulting alkyl radicals react with 4-hydroxyquinazolines or quinones to form 2-alkyldihydroquinazolinones or alkylated quinones. This alternative strategy provides a simple and effective pathway for the construction of C(sp(2))-C(sp(3)) and C(sp(3))-C(sp(3)) bonds under mild conditions.
SYNTHESIS-STUTTGART
(2022)
Review
Biochemistry & Molecular Biology
Jie Zhao, Farong Ye, Ping Huang, Ping Wang
Summary: This review summarizes the recent progress in the synthesis strategies of homogeneous O-glycoproteins, highlighting the significant advancements in the understanding of the relationship between glycan structure and function.
CURRENT OPINION IN CHEMICAL BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Xueyi Liu, Jiazhi Liu, Zhichao Wu, Liangbiao Chen, Siyao Wang, Ping Wang
Article
Chemistry, Organic
Kaiqian Wang, Xiaoping Chen, Xiao Peng, Ping Wang, Feng Liang
ORGANIC & BIOMOLECULAR CHEMISTRY
(2019)
