Article
Chemistry, Multidisciplinary
Lukas Kerul, Maximilian Schrems, Alanca Schmid, Rajeshwari Meli, Christian F. W. Becker, Claudia Bello
Summary: This study describes the establishment of a semi-synthesis strategy for the preparation of modified variants of G-CSF, addressing the issues of low solubility and aggregation. The successful preparation of G-CSF variants with biological activity in cell proliferation assays was achieved.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Review
Dermatology
J. Karim Ead, David G. Armstrong
Summary: GM-CSF is a glycoprotein with immunomodulatory properties derived from both hemopoietic and nonhemopoietic sources. In patients with diabetic foot ulcers, GM-CSF activation is impaired, leading to impaired inflammatory response. Correcting macrophage immune dysfunction with exogenous GM-CSF may help restore immune balance and promote wound healing.
INTERNATIONAL WOUND JOURNAL
(2023)
Article
Virology
Sarah Stonedahl, J. Smith Leser, Penny Clarke, Huntington Potter, Timothy D. D. Boyd, Kenneth L. L. Tyler
Summary: West Nile virus (WNV) is the main cause of epidemic arboviral encephalitis in the United States. Depleting microglia in WNV-infected mice enhances viral replication and tissue injury, while augmenting microglial activation through GM-CSF administration reduces viral titers and apoptotic activity and increases survival. Stimulation of microglial activation may be a viable therapeutic approach for WNV neuroinvasive disease.
JOURNAL OF VIROLOGY
(2023)
Article
Immunology
An-ran Xi, Yi-jun Luo, Jin-Tao Guan, Wei-Jie Wang, Zheng-Hao Xu
Summary: This study analyzed the efficacy and safety of granulocyte-macrophage colony-stimulating factor (GM-CSF) antibodies in COVID-19 patients. The results showed that GM-CSF antibody treatment can reduce the incidence of invasive mechanical ventilation and have slight but not significant effects on mortality and ventilation improvement. Sensitivity analysis revealed statistically significant results for mortality and ventilation improvement rate when one study was excluded. Furthermore, GM-CSF antibodies did not increase the risks of secondary infection and adverse events.
INFLAMMOPHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Shao-Yu Tang, Yi-Chin Lee, Chien-Wei Tseng, Po-Hsun Huang, Ko-Lin Kuo, Der-Cherng Tarng
Summary: This study found that G-CSF can improve angiogenic function in patients with peripheral arterial disease (PAD) associated with chronic kidney disease (CKD) through a hypoxia/IL-10 signaling cascade.
Article
Medicine, General & Internal
Slawomir Lewicki, Monika Lesniak, Agnieszka Sobolewska-Ruta, Aneta Lewicka, Marta Grodzik, Eugeniusz K. Machaj, Marek Saracyn, Jacek Z. Kubiak, Zygmunt Pojda
Summary: Encapsulation of cytokines in liposomes is an efficient method to deliver cytokines to cells at working concentrations without affecting cell morphology or proliferation. This method has great potential and important applications in tissue regeneration and other fields.
ARCHIVES OF MEDICAL SCIENCE
(2022)
Article
Chemistry, Organic
Avijit K. Adak, Yung-Yu Su, Wei-Hao Wang, Chin-Lan Lin, Hao Gu, Yi-Chen Huang, Zhe-Jie Zhang, Chai-Lin Kao, Chun-Cheng Lin
Summary: Photoactivatable ligation auxiliaries simplify protein assembly by cleaving the auxiliary after ligation through photolysis. This study introduces an easy-to-attach TmNpE-auxiliary at the internal side-chain of N-terminal peptide fragments, enabling long-range ligation and photolytic removal in an aqueous buffer. The method's versatility is demonstrated by synthesizing a hPD-L1 peptide inhibitor. The TmNpE group offers easy introduction, compatibility with peptide ligation reactions, and mild cleavage conditions.
Article
Pharmacology & Pharmacy
Jin-Tao Guan, Wei-Jie Wang, Du Jin, Xiao-Yue Mou, Shan-Shan Lei, Zheng-Hao Xu
Summary: This meta-analysis showed that GM-CSF antibody treatment in COVID-19 patients could reduce the risk of death and increase ventilation, without significantly increasing the risk of secondary infection or ICU admission. Older age and length of stay may have an impact on these outcomes.
THERAPEUTIC ADVANCES IN CHRONIC DISEASE
(2021)
Article
Immunology
Peilin Chen, Chunyu Huang, Qing Sun, Huixian Zhong, Feng Xiong, Su Liu, Zhihong Yao, Zhiqiang Liu, Caiyun Wan, Yong Zeng, Lianghui Diao
Summary: This study investigated the concentration of GM-CSF in human single-blastocyst conditioned medium (SBCM) and its association with embryo quality and pregnancy outcome. GM-CSF was found to be a potential biomarker for predicting pregnancy outcome and selecting embryos with high developmental potential for transfer in assisted reproductive technology (ART).
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Gustavo de Souza Vieira, Talita de Carvalho Kimura, Joao Figueira Scarini, Reydson Alcides de Lima-Souza, Luccas Lavareze, Carolina Emerick, Mayara Trevizol Goncalves, Ingrid Iara Damas, Tayna Figueiredo-Maciel, Raisa Sales de Sa, Iara Goncalves Aquino, Joao Paulo Goncalves de Paiva, Patricia Maria Fernandes, Moises Willian Aparecido Goncalves, Luiz Paulo Kowalski, Albina Altemani, Gary Chris Fillmore, Fernanda Viviane Mariano, Erika Said Abu Egal
Summary: This article provides an in-depth review of the role of hematopoietic cytokines in the tumor microenvironment of head and neck cancers, and summarizes the current clinical trials and findings on the therapeutic application of these cytokines.
Article
Biotechnology & Applied Microbiology
Rui Chen, Yujie Li, Yangyang Zhuang, Yiming Zhang, Hailong Wu, Tao Lin, Shixuan Chen
Summary: This study combines GM-CSF and 3D nanofiber scaffolds to promote tissue regeneration. Through subcutaneous implantation in humanized mice, it was found that GM-CSF could accelerate cell migration and cytokine release, and ultimately enhance tissue regeneration. Therefore, GM-CSF loaded 3D nanofiber scaffolds have potential applications in tissue regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Oncology
Lan Huang, Guisi Chen, Ying Chen, Wanwen Wu, Changli Tao, Hongwei Shao, Shulin Huang, Han Shen
Summary: Tumor immunotherapy targeting tumor-associated antigens and the impact of GM-CSF and CCL17 on tumor immunity have been widely studied. Combining survivin-specific CTL with CCL17 and GM-CSF genes can inhibit tumor growth more effectively through stable cell lines expressing these genes.
Article
Dermatology
K. Wolk, T. -C. Brembach, D. Simaite, E. Bartnik, S. Cucinotta, A. Pokrywka, M. L. Irmer, J. Triebus, E. Witte-Haendel, G. Salinas, T. Leeuw, H. -D. Volk, K. Ghoreschi, R. Sabat
Summary: The expression of G-CSF is high in HS skin, especially in inflamed nodules and abscesses. IL-1 and IL-17 induce G-CSF production, and G-CSF blood levels correlate with the severity of HS.
BRITISH JOURNAL OF DERMATOLOGY
(2021)
Article
Biology
Yang Zhou, Leqing Cao, Huidong Guo, Yan Hong, Ming Wang, Ke Wang, Xiaojun Huang, Yingjun Chang
Summary: Our study demonstrates that G-CSF-primed donor T cells have a protective effect against acute graft-versus-host disease (aGVHD) in specific target organs, particularly in the lung and gut. This protection is associated with increased Th2 cells and regulatory T cells in these organs, as well as inhibition of ICOS expression and upregulation of tolerance-related genes. The immune regulatory effects of G-CSF on T cell-mediated aGVHD, especially in GVHD target organs, provide new insights for potential therapeutic strategies.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Medicine, General & Internal
Ying Jiang, Dan Feng, Chun Wang, Yanlei Zhang, Chuxian Zhao, Su Li, Youwen Qin, Alex H. Chang, Jun Zhu
Summary: This study evaluates the safety and efficacy of GM-CSF intervention post-CAR T-cell therapy in patients with neutropenia and concomitant decline of circulating CAR T-cells. The results show that GM-CSF intervention leads to CAR T-cell expansion and increased levels of white blood cells, neutrophils, lymphocytes, and CD3-CD16 + CD56 + natural killer cells in peripheral blood.
FRONTIERS IN MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Shuquan Fan, Wanzhen Li, Kuixing Zhang, Xiangman Zou, Wei Shi, Zhi Liu, Caihong Tang, Wei Huang, Feng Tang
Summary: To improve the efficiency of ENGase-catalyzed glycoengineering process, fusion proteins of AlfC with EndoS/S2 non-catalytic domain were constructed. These fusion proteins showed enhanced defucosylation activity toward deglycosylated IgGs. Kinetic study revealed that EndoS-based fusion proteins had higher kcat values while EndoS2-based ones had lower Km values instead of higher kcat. This research provides an effective approach to improve AlfC activity and shorten the defucosylation process, which will significantly promote the development of glycoengineered antibodies in the future.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Chemistry, Medicinal
Bo Liu, Xueni Yu, Liping Liu, Lei Wang, Jie Wang, Qianqian Huang, Zhongliang Xu, Cheng Luo, Liguang Lou, Wei Huang, Weibo Yang
Summary: This study presents a modular biomimetic strategy for the efficient synthesis of a new class of macrocycles with polysubstituted 1,3-diene, which exhibit potent inhibition of P-gp and reversal of multidrug resistance. Structure-activity relationship analysis reveals that the size and linker of the macrocycles are crucial for restoring activity. Compound 32, containing a naphthyl group and (D)-Phe moiety, exhibits higher potency than verapamil and induces conformational change of P-gp to inhibit its function. Compounds 23 and 32 are identified as attractive leads, showing good pharmacokinetic profile and antitumor activity in a KBV200 xenograft mouse model.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Medicinal
Wei Shi, Jianxin Zhang, Liya Liu, Wanzhen Li, Zhi Liu, Anni Ren, Jie Wang, Caihong Tang, Yang Yang, Dandan Xu, Qianqian Huang, Yongqin Wang, Caili Luo, Wei Huang, Feng Tang
Summary: The inadequate understanding of the structure-activity relationship (SAR) of glycosite-specific antibody-drug conjugates (ADCs) hinders its design and development. In this study, we constructed and examined 50 gsADC structures with various glycan subtypes and linker-drug combinations to reveal the systematic SAR and structure-toxicity relationship (STR) of gsADCs. Our findings suggest that the use of extra hydrophilic linkers is crucial for intact glycan-based gsADCs to improve in vivo efficacy. Additionally, gsADCs that conjugate linker-drug complexes onto the terminal sialic acid demonstrate greater stability and potency compared to those conjugated onto the terminal galactose. Moreover, LacNAc-based gsADCs, which adopt a shorter spacer and locate the linker-drug more inside the immunoglobulin class G (IgG) Fc cavity, exhibit excellent hydrophilicity, in vivo activity, pharmacokinetics, and safety. Overall, we found that concealing the linker-toxin within the Fc cavity can significantly enhance the therapeutic index of LacNAc-based gsADCs, facilitating the design of ADCs with optimal druggability.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Qixiong Lu, Xiaoyang Lu, Yuansheng Zhang, Wei Huang, Hu Zhou, Tao Li
Summary: Ferroptosis is a form of cell death characterized by excessive iron-dependent lipid peroxidation. It disrupts the cellular antioxidant capacities and leads to oxidative overload and cell death. Ferroptosis plays a significant role in various diseases.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Wei Huang, Jiahui Bai, Yinlong Guo, Qinglei Chong, Fanke Meng
Summary: A catalytic enantioselective coupling of 1,1-disubstituted allenes and aldehydes is achieved through regiodivergent oxidative cyclization followed by stereoselective protonation or reductive elimination promoted by chiral phosphine-Co complexes. This unprecedented reaction pathway for Co catalysis allows for the catalytic enantioselective generation of metallacycles with divergent regioselectivity accurately controlled by chiral ligands, leading to a wide range of allylic alcohols and homoallylic alcohols in high yields and excellent regioselectivity, diastereoselectivity, and enantioselectivity.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemical Research Methods
Xing Ling, Sixiu Liu, Yixuan Yang, Qian Dong, Lisa A. Marcaurelle, Wei Huang, Yun Ding, Xuan Wang, Xiaojie Lu
Summary: Carbohydrates are important natural active products that regulate various physiological activities. To meet the demand for potential drug candidates, we developed on-DNA protocols to construct DNA-encoded glycoconjugates. These chemistries enable the generation and modification of DNA-linked glycosyl compounds with good conversions and broad substrate scope. Three DNA-linked glycoconjugate libraries were successfully generated to demonstrate their applicability and feasibility in library preparation.
BIOCONJUGATE CHEMISTRY
(2023)
Article
Biochemical Research Methods
Caihong Tang, Yue Zeng, Jianxin Zhang, Xing Zheng, Feng Tang, Xu Yao, Zhong-Xing Jiang, Wei Shi, Wei Huang
Summary: In this study, a facile and efficient method was developed to assemble dual-site-specific ADCs with defined DARs at both the N-glycosylation site and K248 site, either with the same payloads or with two types of payloads. The constructed dual-site ADCs showed acceptable homogeneity, excellent buffer stability, and enhanced in vitro and in vivo efficiency.
BIOCONJUGATE CHEMISTRY
(2023)
Article
Chemistry, Medicinal
Dongliang Guan, Feifei Chen, Wei Shi, Lefu Lan, Wei Huang
Summary: The authors synthesized 40 novel norvancomycin derivatives, of which 32 were single N-terminally modified derivatives obtained through simple and efficient methods. The diverse N-terminal modifications were mainly achieved by lipophilic attachment and introduction of lipo-sulfonium moieties for extensive structure-activity relationship analysis. The first incorporation of a sulfonium moiety into the norvancomycin structure resulted in compounds with 4- to 2048-fold higher activity against vancomycin-resistant bacteria VISA and VRE. This N-terminal modification for norvancomycin provides a useful and promising strategy to restore the antibacterial activity of glycopeptide antibiotics against resistant bacteria, highlighting the same importance of the N-terminal site as well as the vancosamine position, which deserves further study and development.
Article
Biochemistry & Molecular Biology
Haihua He, Chonglai Chen, Chaoqun Bian, Junhua Ren, Jiajia Liu, Wei Huang
Summary: Ammonia decomposition is a promising method for high-purity hydrogen production, but it typically requires precious metals as catalysts. This study proposes using Ni/GdxCe1-xO2-delta catalysts to improve performance by adjusting support properties and investigates the underlying mechanism. It is found that Ni/Ce0.8Gd0.2O2-delta exhibits high catalytic activity and stability due to a high concentration of oxygen vacancies, highly dispersed Ni, and abundant strong basic sites.
Article
Biochemistry & Molecular Biology
Wei Huang, Yuliang Wu, Jihui Zhu, Ning Luo, Chunyan Wang, Shupeng Liu, Zhongping Cheng
Summary: This study investigated the expression features of FDX1 in tumors and its correlations with prognosis, tumor stages, immune microenvironment, and cuproptosis from a pan-cancer perspective based on integrated bioinformatics. The results showed that FDX1 was abnormally expressed in multiple tumor types and demonstrated variability in various tumor stages. It predicted poor prognosis in glioma (GBMLGG) and brain lower-grade glioma (LGG), and good prognosis in kidney renal clear cell carcinoma (KIRC). FDX1 showed positive correlations to StromalScore, ImmuneScore, ESTIMATEScore in LGG and negative correlation in KIRC. High expression of FDX1 was accompanied by high expression of immune checkpoints such as CD276 (B7-H3), CD274 (PD-L1), PDCD1LG2 (PD-L2), CTLA4, and HAVCR2 in LGG.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Sen Zhou, Ma Bo, Feng Tang, Wenqiang Liu, Xin Li, Baoquan Chen, Shiying Shang, Wei Huang, Yaohao Li, Zhongping Tan
Summary: This study focuses on the synthesis of GlcNAc oxazoline mediated by CDMBI. A previously unreported by-product was discovered and characterized through research. A new strategy was developed to prevent its occurrence by proposing a mechanism for its formation. These findings are important for gaining a deeper understanding of the reaction and may provide a new avenue for optimizing oxazoline synthesis.
NEW JOURNAL OF CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Jie Zhao, Farong Ye, Ping Huang, Ping Wang
Summary: This review summarizes the recent progress in the synthesis strategies of homogeneous O-glycoproteins, highlighting the significant advancements in the understanding of the relationship between glycan structure and function.
CURRENT OPINION IN CHEMICAL BIOLOGY
(2023)
