Article
Plant Sciences
Magda F. El-Adl, Taha M. El-Katony, Reham M. Nada
Summary: The study found that the specific ion effects of Na+ and K+ were different on the growth of Ulva lactuca. Ulva lactuca showed better growth with high concentrations of NaCl, while KCl might have adverse effects on photosynthesis and cell wall. Transcription of PM H+-ATPase was induced specifically by high concentrations of Na+, while the induction of PM-Na+/K+-ATPase-like alone could not improve the growth of KCl-treated Ulva lactuca.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Tomas Heger, Marek Zatloukal, Martin Kubala, Miroslav Strnad, Jiri Gruz
Summary: A newly discovered trimeric procyanidin C1, found in Viola odorata root extract, was shown to strongly inhibit Na+,K+-ATPase activity. Leaf and seed extracts of the plant showed no significant inhibitory effects on the enzyme. Through compound screening and bioactivity assessment, the active components of V. odorata root extract were identified.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Wei Chen, Lei Wang, Pengfei Liang, Jason Mast, Clausell Mathis, Catherine Y. Liu, Jin Wei, Jie Zhang, Liying Fu, Luis A. Juncos, Jacentha Buggs, Ruisheng Liu
Summary: This study demonstrates the potential therapeutic effect of using a synchronization modulation electric field to activate Na/K pumps and maintain cellular functions during renal ischemia-reperfusion injury.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Plant Sciences
Qi Chen, Kai Xu, Yan Xu, Dehua Ji, Changsheng Chen, Chaotian Xie, Wenlei Wang
Summary: This study analyzed the function of Na+/K+-ATPase in the response of Neoporphyra haitanensis to salt stress and found that it played a crucial role in maintaining the K+/Na+ homeostasis in the algae. The inhibitor ouabain caused Na+ accumulation and K+ leakage in the cells, disrupting the K+/Na+ balance and leading to decreased photosynthesis and oxidative damage in the algae.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Marlene U. Fruergaard, Ingrid Dach, Jacob L. Andersen, Mette Ozol, Azadeh Shahsavar, Esben M. Quistgaard, Hanne Poulsen, Natalya U. Fedosova, Poul Nissen
Summary: In this study, the crystal structure and functional properties of the E2-BeFx complex of pig kidney Na+,K+-ATPase are investigated. The complex resembles the E2P intermediate and is able to bind ADP/ATP and Na+. The results suggest a relation between E2-BeFx and the E*P intermediate in the functional cycle of Na+, K+-ATPase.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Santiago E. Faraj, Wanda M. Valsecchi, Mariela Ferreira-Gomes, Mercedes Centeno, Elina Malen Saint Martin, Natalya U. Fedosova, Juan Pablo FC. Rossi, Monica R. Montes, Rolando C. Rossi
Summary: The Na+/K+-ATPase is a plasma membrane glycoprotein that exchanges intracellular Na+ for extracellular K+ by hydrolyzing ATP. The asymmetric distribution of Na+ and K+ is essential for cellular life. Current research indicates that Na+ becomes spontaneously occluded in the E1 dephosphorylated form of the Na+/K+-ATPase, showing positive interactions between binding sites. Adding ATP does not increase Na+ occlusion.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Review
Microbiology
Cassiano Felippe Goncalves-de-Albuquerque, Carolina Medina Coeli da Cunha, Leo Victor Grimaldi de Castro, Caroline de Azevedo Martins, Marcos Roberto Colombo Barnese, Patricia Burth, Mauricio Younes-Ibrahim
Summary: In 1915, Inada and Ido identified Leptospira sp. as the pathogen responsible for Weil's Disease, which was later confirmed to cause leptospirosis. The molecular mechanisms and interactions between bacterial components and the host, as well as the systemic dysfunctions, still need to be fully understood. Leptospirosis affects all animal species and involves a cellular mechanism common to all eukaryotes. Na/K-ATPase is targeted by the leptospiral endotoxin, leading to dysfunctions and organ disorders. Additionally, metabolic disorders and inflammatory responses are triggered during leptospirosis, involving various molecular mechanisms. Na/K-ATPase plays a critical role in the disease and can be a potential therapeutic target or an indicator of disease severity.
Article
Cell Biology
Bruno Sterlini, Alessandra Romei, Chiara Parodi, Davide Aprile, Michele Oneto, Anita Aperia, Pierluigi Valente, Flavia Valtorta, Anna Fassio, Pietro Baldelli, Fabio Benfenati, Anna Corradi
Summary: This study reveals that PRRT2 deficiency affects the function of Na+/K+ ATPase (NKA) in neurons, leading to increased clustering of NKA on the plasma membrane, which can be reversed by re-expression of PRRT2. Additionally, PRRT2 deficiency also reduces the NKA-dependent afterhyperpolarization in neurons following high-frequency firing.
CELL DEATH & DISEASE
(2021)
Article
Food Science & Technology
Anna Tama, Grzegorz Bartosz, Izabela Sadowska-Bartosz
Summary: Polyphenols in wines autoxidize to produce hydrogen peroxide upon contact with air, with the net production influenced by both production and scavenging of hydrogen peroxide in the wine.
Article
Immunology
Jin Zheng, Ping Lan, Meihe Li, Min-chao Kang, Meng Xun, Xiangyun Ma, Mengyao Yan, Dan Sun, Yu Shen, Xinyi Fu, Xiaoming Ding, Xiaofei Yan, Wu-jun Xue
Summary: Reduced Na+/K+-ATPase (NKA) activity and NKA alpha 1 expression are implicated in the pathogenesis of renal diseases. NKA alpha 1 deficiency exacerbates tubulointerstitial lesions and fibrosis in a mouse model of UUO. Activation of NKA alpha 1 by an antibody against its extracellular DR region (DRm217) prevents UUO-induced tubulointerstitial lesions, preserves kidney function, and reduces renal fibrosis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Chemistry, Medicinal
Lucy Kate Ladefoged, Birgit Schiott, Natalya U. Fedosova
Summary: The study reveals significant differences in the binding properties of different subfamilies of cardiotonic steroids with the Na+,K+-ATPase. While bufadienolides show beneficial effects with K+, they differ from cardenolides which have an antagonistic relationship with K+. Through simulations, it was found that the binding of bufadienolides is influenced by amino acid coordination patterns and the presence of cations, illustrating the complex interplay of factors in these interactions.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2021)
Article
Multidisciplinary Sciences
Victoria C. Young, Hanayo Nakanishi, Dylan J. Meyer, Tomohiro Nishizawa, Atsunori Oshima, Pablo Artigas, Kazuhiro Abe
Summary: This study investigates ion transport mechanisms by introducing mutations to convert an electroneutral proton/potassium pump into a prototypical electrogenic sodium/potassium pump, explaining their selectivity and phosphorylation mechanisms.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Malgorzata Janicka, Malgorzata Reda, Natalia Napieraj, Adrianna Michalak, Dagmara Jakubowska, Katarzyna Kabala
Summary: Cucumber plants can reduce the toxic effects of Cd through removing excess toxic ions and increasing the activity of PM H+-ATPase under cadmium stress. This process is influenced by the activity of DAO, which promotes the formation of NO through H2O2 production. The stimulating effect of Cd on PM H+-ATPase activity and expression of CsHA2, CsHA4, and CsHA8 genes can be inhibited by the DAO inhibitor AG.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Victoria C. Young, Pablo Artigas
Summary: Utilizing state-specific engineered-disulfide cross-linking, it was demonstrated that the transmembrane segment 2 (M2) of the Na+/K+ pump moves in directions inconsistent with distances observed in existing crystal structures of the pump. This movement was characterized using voltage-clamp fluorometry in single-cysteine mutants, revealing state-dependent fluorescence changes upon labeling with tetramethylrhodamine-6-maleimide (TMRM).
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Neurosciences
Jinyi Sun, Yang Zheng, Zhong Chen, Yi Wang
Summary: This review comprehensively summarizes the role of Na+-K+-ATPase in epilepsy and provides perspectives on possible research directions and therapeutic targets.
CNS NEUROSCIENCE & THERAPEUTICS
(2022)
Article
Multidisciplinary Sciences
Thais Russo-Abrahao, Marco Antonio Lacerda-Abreu, Taina Gomes, Daniela Cosentino-Gomes, Ayra Diandra Carvalho-de-Araujo, Mariana Figueiredo Rodrigues, Ana Carolina Leal de Oliveira, Franklin David Rumjanek, Robson de Queiroz Monteiro, Jose Roberto Meyer-Fernandes
Article
Biochemistry & Molecular Biology
Yukio Nisimoto, Becky A. Diebold, Daniela Constentino-Gomes, J. David Lambeth
Article
Parasitology
Daniela Cosentino-Gomes, Nathalia Rocco-Machado, Jose Roberto Meyer-Fernandes
EXPERIMENTAL PARASITOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Michele Alves-Bezerra, Daniela Cosentino-Gomes, Lisvane P. Vieira, Nathalia Rocco-Machado, Katia C. Gondim, Jose R. Meyer-Fernandes
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Marco Antonio Lacerda-Abreu, Thais Russo-Abrahao, Daniela Cosentino-Gomes, Michelle Tanny Cunha Nascimento, Luiz Fernando Carvalho-Kelly, Taina Gomes, Mariana Figueiredo Rodrigues, Sandra Konig, Franklin David Rumjanek, Robson Q. Monteiro, Jose Roberto Meyer-Fernandes
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2019)
Article
Parasitology
Andre L. A. Dos-Santos, Claudia F. Dick, Leandro R. Lopes, Nathalia Rocco-Machado, Humberto Muzi-Filho, Anita L. Freitas-Mesquita, Lisvane Paes-Vieira, Adalberto Vieyra, Jose Roberto Meyer-Fernandez
EXPERIMENTAL PARASITOLOGY
(2019)
Article
Biochemistry & Molecular Biology
N. Rocco-Machado, D. Cosentino-Gomes, M. T. Nascimento, L. Paes-Vieira, Y. A. Khan, B. Mittra, N. W. Andrews, J. R. Meyer-Fernandes
FREE RADICAL BIOLOGY AND MEDICINE
(2019)
Article
Biophysics
Luiz Fernando Carvalho-Kelly, Clara Ferreira Pralon, Nathalia Rocco-Machado, Michelle Tanny Nascimento, Ayra Diandra Carvalho-de-Araujo, Jose Roberto Meyer-Fernandes
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
(2020)
Article
Biochemistry & Molecular Biology
Marco Antonio Lacerda-Abreu, Thais Russo-Abrahao, Nathalia Rocco-Machado, Daniela Cosentino-Gomes, Claudia Fernanda Dick, Luiz Fernando Carvalho-Kelly, Michelle Tanny Cunha Nascimento, Thais Cristino Rocha-Vieira, Jose Roberto Meyer-Fernandes
Summary: High extracellular Pi concentration can induce ROS production in TNBC (MDA-MB-231), leading to mitochondrial hyperpolarization, increased ROS generation, impaired oxygen consumption, and elevated PKC activity. The source of H2O2 production shifts from mitochondria to NADPH oxidase after prolonged Pi exposure, while exogenous H2O2 has similar effects on Pi-transporters as high Pi levels. Antioxidants like NAC can reverse these effects and inhibit Pi-induced EMT, migration, and adhesion in MDA-MB-231 cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Brian S. Mantilla, Lisvane Paes-Vieira, Felipe de Almeida Dias, Simone G. Calderano, Maria Carolina Elias, Daniela Cosentino-Gomes, Pedro L. Oliveira, Jose Roberto Meyer-Fernandes, Ariel M. Silber
Summary: The pathogenic protist Trypanosoma cruzi utilizes kissing bugs as invertebrate hosts to vectorize infection among mammals. Through the proline-glutamate oxidation pathway, mitochondrial enzymes play a crucial role in differentiation and energy production in the parasite. Overexpression of these enzymes leads to increased mitochondrial functions and different oxygen signaling, influencing parasitic differentiation efficiency in the invertebrate host.
BIOCHEMICAL JOURNAL
(2021)
Article
Parasitology
Marta Teixeira Gomes, Lisvane Paes-Vieira, Andre Luiz Gomes-Vieira, Daniela Cosentino-Gomes, Ana Paula Pereira da Silva, Naira Ligia Lima Giarola, Daniel Da Silva, Mauro Sola-Penna, Antonio Galina, Jose Roberto Meyer-Fernandes
Summary: 3-Bromopyruvate (3-BrPA) significantly reduced glycolytic enzyme activity in L. amazonensis, leading to decreased ATP levels and energy depletion, thereby inhibiting cell proliferation and infectivity.
EXPERIMENTAL PARASITOLOGY
(2021)