Review
Chemistry, Multidisciplinary
Chang Liu, Jinghui Luo
Summary: Protein oligomers are abundant in nature and have important roles in physiology and pathology. Their complex structure and conformational changes make it difficult to study them in detail. This article classifies and describes oligomers based on biological function, toxicity, and application. It also reviews recent advancements and methods for engineering protein oligomers, highlighting protein grafting as a promising approach. These developments bring us closer to understanding the biological functions, toxicity, and potential applications of stabilized oligomers.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Medicine, Research & Experimental
Julia Ring, Jelena Tadic, Selena Ristic, Michael Poglitsch, Martina Bergmann, Nemanja Radic, Dirk Mossmann, Yongtian Liang, Marta Maglione, Andrea Jerkovic, Roozbeh Hajiraissi, Marcel Hanke, Victoria Kuettner, Heimo Wolinski, Andreas Zimmermann, Lana Domuz Trifunovic, Leonie Mikolasch, Daiana N. Moretti, Filomena Broeskamp, Julia Westermayer, Claudia Abraham, Simon Schauer, Christopher Dammbrueck, Sebastian J. Hofer, Mahmoud Abdellatif, Guido Grundmeier, Guido Kroemer, Ralf J. Braun, Niklas Hansen, Cornelia Sommer, Mirjana Ninkovic, Sandra Seba, Patrick Rockenfeller, Friederike-Nora Voegtle, Joern Dengjel, Chris Meisinger, Adrian Keller, Stephan J. Sigrist, Tobias Eisenberg, Frank Madeo
Summary: In this study, the researchers used yeast and fruit fly models to investigate the role of HSP40 family member Ydj1 in Alzheimer's disease. They found that Ydj1 physically interacts with Abeta42, stabilizes Abeta42 oligomers, and facilitates their translocation to mitochondria, resulting in cell death and memory impairment.
EMBO MOLECULAR MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Yi Wen, Volker M. Vogt, Gerald W. Feigenson
Summary: Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P-2) is a crucial component located at the inner leaflet of the cell membrane, constituting only 1-2% of total membrane lipids. Its synthesis and turnover are spatially and temporally regulated. Various factors, such as multivalent cations and cellular proteins interacting with PI(4,5)P-2, contribute to its versatile and dynamic distribution within membranes.
ANNUAL REVIEW OF BIOCHEMISTRY, VOL 90, 2021
(2021)
Article
Multidisciplinary Sciences
Veronika Thallmair, Lea Schultz, Wencai Zhao, Siewert J. Marrink, Dominik Oliver, Sebastian Thallmair
Summary: Phosphoinositides (PIs), especially phosphatidylinositol 4,5-bisphosphate [PI(4,5)P-2], play a crucial role in targeting proteins to cilia. This study discovered that the tubby domain, which is essential for this targeting process, binds to PI(4,5)P-2 through two binding sites.
Article
Multidisciplinary Sciences
Liangqian Huang, Trisha Agrawal, Guixin Zhu, Sixiang Yu, Liming Tao, JiaBei Lin, Ronen Marmorstein, James Shorter, Xiaolu Yang
Summary: Protein quality control systems are crucial for cellular function and organismal health. Recent studies have shown that polyD/E proteins, such as DAXX, ANP32A, and SET, may constitute a multifunctional protein quality control system that operates via a distinctive mechanism without relying on ATP.
Article
Biochemistry & Molecular Biology
Meenakshi Pillai, Santosh Kumar Jha
Summary: TDP-43 protein is associated with neurodegenerative diseases and can adopt different oligomeric and fibrillar states. However, the kinetic understanding of its structural transformation is still lacking. This study reveals that aggregation of TDP-43 occurs in four distinct steps, involving conformational conversion, oligomerization, and formation of higher order intermediates (HOI), before ultimately forming an amyloid-like assembly. The formation of ThT binding sites and conformational conversion depends on the protein concentration. The aggregation process follows a linear polymerization reaction without a lag phase.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2023)
Article
Biochemistry & Molecular Biology
Maiwase Tembo, Rachel E. Bainbridge, Crystal Lara-Santos, Kayla M. Komondor, Grant J. Daskivich, Jacob D. Durrant, Joel C. Rosenbaum, Anne E. Carlson
Summary: This study investigates the interaction between TMEM16A channel and the membrane lipid phosphatidylinositol 4,5-bisphosphate (PI(4,5)P-2). The findings show that only lipids containing a phosphate at the 40 position effectively recover TMEM16A currents. These findings improve our understanding of how PI(4,5)P-2 binds to and potentiates TMEM16A channels.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Wen Xu, Jinfei Mei, Chuanbo Wang, Huijuan Yang, Xiaohong Ma, Wenqi Gao, Sajjad Ahmad, Hongqi Ai
Summary: Fibrous aggregates of beta-amyloid (Aβ) are a hallmark of Alzheimer's disease (AD). A pair of positive and negative ions is emerging as a powerful dual-inhibitor candidate for inhibiting the misfolding or aggregation of Aβ42. This study used ion pairs of oppositely charged benzothiazole molecules at different pH conditions to bind Aβ42 targets and elucidated the inhibitory mechanisms and binding strength of the dual-inhibitor. The study also revealed that solvation played a critical role in the enhancement of the dual-inhibitor binding to Aβ42.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Cell Biology
Harry Scott, Li Dong, Andrew Stevenson, Alasdair I. MacDonald, Sharmila Srinivasan, Paola Massimi, Lawrence Banks, Patricia E. Martin, Scott R. Johnstone, Sheila Graham
Summary: This study identifies the interaction between Cx43 and Dlg1 in uninfected keratinocytes, which plays a crucial role in maintaining Cx43 distribution on the plasma membrane, thus affecting intercellular communication through gap junctions.
JOURNAL OF CELL SCIENCE
(2023)
Article
Chemistry, Physical
William B. Weeks, Lauren E. Buchanan
Summary: This article presents a method to distinguish multiple β-sheet structures within peptide and protein aggregates, and demonstrates its effectiveness through experiments. The method utilizes two-dimensional infrared spectroscopy to calculate the transition dipole strength (TDS) spectrum, which provides higher sensitivity to protein structure and can detect structural details that are not observable by other techniques.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Review
Biochemistry & Molecular Biology
Martyna M. Matuszyk, Claire J. Garwood, Laura Ferraiuolo, Julie E. Simpson, Rosemary A. Staniforth, Stephen B. Wharton
Summary: This article discusses the role of amyloid in Alzheimer's disease, explores factors leading to inconsistency in research methods, and analyzes different techniques for studying A beta. The study further underscores the need for standardization in A beta preparations and methods in current research.
JOURNAL OF NEUROCHEMISTRY
(2022)
Article
Plant Sciences
Eun-Ha Kim, Roshan Sharma Poudyal, Kyeong-Ryeol Lee, Hami Yu, Eunji Gi, Hyun Uk Kim
Summary: Recent studies have demonstrated the important role of CPSFL1/PITP7 in photoautotrophic growth and chloroplast vesicle formation. This study further investigated the functional roles of CPSFL1/PITP7 using A. thaliana mutants and found that PITP7 transcript levels were closely related to mutant phenotypes. The study also identified the binding affinity of PITP7 to PIPs and proposed a possible role for PITP7 in membrane trafficking of hydrophobic ligands.
PHYSIOLOGIA PLANTARUM
(2022)
Article
Chemistry, Medicinal
Yunxiang Sun, Junchao Huang, Xiangmei Duan, Feng Ding
Summary: The comparison of self-assembly dynamics and structures of SOD1(28-38) and its nontoxic mutants revealed distinct aggregation pathways and intermediates, supporting beta-barrels as the toxic oligomers in amyloidosis. The well-defined structures of beta-barrels may serve as a novel therapeutic target against amyloid-related diseases.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2021)
Article
Multidisciplinary Sciences
Ricarda Torner, Tatsiana Kupreichyk, Lothar Gremer, Elisa Colas Debled, Daphna Fenel, Sarah Schemmert, Pierre Gans, Dieter Willbold, Guy Schoehn, Wolfgang Hoyer, Jerome Boisbouvier
Summary: Integrated kinetic and structural investigations reveal that the co-chaperonin prefoldin interacts with islet amyloid polypeptide fibrils to inhibit their elongation and secondary nucleation.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Cagla Sahin, Axel Leppert, Michael Landreh
Summary: Membrane-less organelles assemble through liquid-liquid phase separation of proteins, and mass spectrometry (MS) offers promising approaches for studying protein phase separation. This Perspective discusses recent advances and proposes integrating MS into the experimental workflow for phase separation studies, while also addressing challenges and future opportunities.
Article
Biochemistry & Molecular Biology
Oksana S. Forsova, Vladislav V. Zakharov
Article
Developmental Biology
F. M. Zakharova, V. V. Zakharov
RUSSIAN JOURNAL OF DEVELOPMENTAL BIOLOGY
(2017)
Article
Medicine, Research & Experimental
V. V. Zakharov, Yu. K. Abramova, M. I. Mosevitsky
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
(2011)
Article
Biochemistry & Molecular Biology
Olga S. Ostroumova, Ludmila V. Schagina, Mark I. Mosevitsky, Vladislav V. Zakharov
Meeting Abstract
Biochemistry & Molecular Biology
S. S. Efimova, V. V. Zakharov, O. S. Ostroumova
Meeting Abstract
Biochemistry & Molecular Biology
O. S. Vitiuk, N. Y. Giliano, V. V. Zakharov
Meeting Abstract
Biochemistry & Molecular Biology
O. S. Vitiuk, N. Y. Giliano, V. V. Zakharov
JOURNAL OF NEUROCHEMISTRY
(2013)
Meeting Abstract
Biochemistry & Molecular Biology
F. M. Zakharova, V. V. Zakharov
JOURNAL OF NEUROCHEMISTRY
(2013)
Meeting Abstract
Biochemistry & Molecular Biology
F. M. Zakharova, V. V. Zakharov
Article
Biochemistry & Molecular Biology
VV Zakharov, MN Bogdanova, MI Mosevitsky
BIOCHEMISTRY-MOSCOW
(2005)
Meeting Abstract
Biochemistry & Molecular Biology
VV Zakharov, MI Mosevitsky
JOURNAL OF NEUROCHEMISTRY
(2005)
Meeting Abstract
Biochemistry & Molecular Biology
MI Mosevitsky, VV Zakharov, IA Silicheva, NE Belova
JOURNAL OF NEUROCHEMISTRY
(2005)
Meeting Abstract
Biochemistry & Molecular Biology
VV Zakharov, MN Bogdanova, MI Mosevitsky
JOURNAL OF NEUROCHEMISTRY
(2004)
Article
Biochemistry & Molecular Biology
Vladislav V. Zakharov, Mark I. Mosevitsky
JOURNAL OF NEUROCHEMISTRY
(2007)