Article
Multidisciplinary Sciences
Byomkesh Dash, Sudhansu Sekhar Bhuyan, Sandeep Kumar Singh, Manjusha Chandravani, Nibedita Swain, Prachitara Rout, Jawahar Lal Katara, C. Parameswaran, B. N. Devanna, Sanghamitra Samantaray
Summary: Rice is crucial for global food security. This study developed an androgenic protocol for indica rice, contributing to yield improvement.
Article
Plant Sciences
Monika Gajecka, Marek Marzec, Beata Chmielewska, Janusz Jelonek, Justyna Zbieszczyk, Iwona Szarejko
Summary: This study investigated the genotype-dependent molecular differences in chloroplast differentiation leading to the formation of green and albino regenerants in microspore culture of barley. The results showed that the failed transition from NEP-dependent to PEP-dependent transcription in plastids was associated with albino regenerants, while a high activity of PEP polymerase and increased expression of certain genes correlated with the production of green regenerants. Understanding these molecular mechanisms may help overcome the issue of albinism in cereal androgenesis.
Review
Biology
Ricardo Mir, Antonio Calabuig-Serna, Jose M. Segui-Simarro
Summary: This review compiles the latest advances in doubled haploid plant production, the application of doubled haploid lines in breeding programs, and future perspectives for alternative technologies for doubled haploid generation in eggplant. Eggplant is globally cultivated for its edible fruit, and breeding programs aim to produce F1 hybrids by crossing highly homozygous pure lines. Doubled haploid individuals can be induced from haploid cells in a single generation, offering a more efficient alternative to traditional self crossing methods.
Review
Agronomy
Agnieszka Kielkowska, Waldemar Kiszczak
Summary: This paper presents the historical background and current status of haploid induction in carrot, and discusses the potential of haploidization technology in carrot breeding. The methods of androgenesis, gynogenesis, and induced parthenogenesis, as well as the manipulation of the centromere-specific histone 3 variant (CENH3) in carrot, are also acknowledged.
Review
Plant Sciences
Brett Hale, Alison M. R. Ferrie, Sreekala Chellamma, J. Pon Samuel, Gregory C. Phillips
Summary: Androgenesis is widely used in plant breeding programs to redirect cell fate and study cell cycle regulation, meiotic recombination, and embryogenesis. The focus has shifted from protocol development to understanding how protocols work in order to extend their application to other genotypes and species. Transcriptomic and epigenetic analyses have revealed cellular and molecular responses associated with androgenesis, and gene transfer and genome editing technologies show promise for enhancing the genetic composition of microspores used in breeding.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Antonio Calabuig-Serna, Carolina Camacho-Fernandez, Ricardo Mir, Rosa Porcel, Esther Carrera, Isabel Lopez-Diaz, Jose M. Segui-Simarro
Summary: During eggplant microspore embryogenesis, the transition from embryos to calli instead of developing as true embryos is a bottleneck. Studies show that there are no genotype-specific endogenous GR profiles associated with a high embryogenic response. The role of GRs in the development of microspore-derived embryos in eggplant cultures is highlighted, with different GR accumulation patterns influencing the embryogenic response.
PLANT GROWTH REGULATION
(2022)
Article
Horticulture
Jialei Ji, Henan Su, Wenxue Cao, Xiaoli Zhang, Hongrun Li, Zhiyuan Fang, Limei Yang, Yangyong Zhang, Mu Zhuang, Yong Wang, Vasiliy Taranov, Honghao Lv
Summary: In this study, QTL mapping was conducted using 109 DH lines derived from a cross between the highly embryogenic genotype 01-88 and the nonembryogenic genotype 02-12. The results showed that a QTL locus controlling cabbage embryogenesis ability was mapped to an interval of 57.4-58.4 Mb on chromosome C03. Furthermore, 12 genes with nonsynonymous mutations were identified in the candidate interval, with the DEFECTIVE KERNEL1 (DEK1) gene being identified as an important candidate gene. This study provides a foundation for cloning genes controlling cabbage microspore embryogenesis and the application of microspore culture technology in cabbage breeding on a large scale.
SCIENTIA HORTICULTURAE
(2023)
Review
Biotechnology & Applied Microbiology
Serik Eliby, Sara Bekkuzhina, Olena Kishchenko, Gulnur Iskakova, Gulnar Kylyshbayeva, Satyvaldy Jatayev, Kathleen Soole, Peter Langridge, Nikolai Borisjuk, Yuri Shavrukov
Summary: Doubled haploid production is a valuable biotechnology that accelerates the breeding of new wheat varieties and plays important roles in studying genetic control, marker-assisted selection, genomics, and genetic engineering in wheat. This paper reviews recent advances in androgenesis and gynogenesis techniques, with a focus on the in vitro culture phase, and the emerging innovative approaches in researching and producing wheat doubled haploids. The use of CRISPR-based genome editing for inducing doubled haploids in wheat is also discussed.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Biochemistry & Molecular Biology
Iwona Zur, Przemyslaw Kopec, Ewa Surowka, Ewa Dubas, Monika Krzewska, Anna Nowicka, Franciszek Janowiak, Katarzyna Juzon, Agnieszka Janas, Balazs Barna, Jozsef Fodor
Summary: Enhanced antioxidant defence is crucial for plant survival under stress conditions, but excessive activity can sometimes suppress the necessary signals for biological reactions. Microspore embryogenesis is triggered by stress in immature male gametophytes, with different stress treatments leading to genotype-specific defence responses in triticale and barley.
Article
Biochemistry & Molecular Biology
Zhiwei Chen, Qi Jiang, Guimei Guo, Qiufang Shen, Jun Yang, Ertao Wang, Guoping Zhang, Ruiju Lu, Chenghong Liu
Summary: Obtaining homozygous lines from transgenic plants is crucial for phenotype evaluation, but the current selection process is time-consuming. By using microspore culture, this study successfully obtained 24 homozygous doubled haploid (DH) transgenic plants from one T-0 transgenic plant overexpressing the HvPR1 gene. The established method allows for rapid evaluation of transgenic lines for gene function studies and trait evaluation, such as analyzing NUE-related research in barley using the HvPR1 overexpression DH lines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biotechnology & Applied Microbiology
Madhu Patial, Ruchi Chauhan, Harinder K. Chaudhary, Kallol K. Pramanick, Arun K. Shukla, Vishnu Kumar, Ramesh P. S. Verma
Summary: The development and improvement of biotechnological tools have greatly contributed to the progress in barley breeding program. The use of doubled haploids has significantly shortened the cultivar development time and has been widely used in various genetic studies. However, challenges such as low efficiency and genotypic specificity still need to be addressed.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Horticulture
Jiaqi Zou, Xiao Zou, Zhichao Gong, Gengxing Song, Jie Ren, Hui Feng
Summary: Curly kale, known for its rich nutrition and health benefits, is highly sought after and considered one of the healthiest vegetables. However, conventional breeding methods for this vegetable take a long time to produce homozygous inbred lines. In this study, the isolated microspore culture (IMC) technique was successfully used to accelerate the breeding cycle of curly kale through efficient cytokinin thidiazuron (TDZ) treatment. The results showed that 0.2 mg/L TDZ had the optimal effect on embryo survival and plant regeneration, significantly shortening the breeding cycle.
Review
Agriculture, Multidisciplinary
T. R. Grigolava, A. V. Vishnyakova, A. A. Sinitsyna, A. V. Voronina, O. N. Zubko, O. Zudova, S. G. Monakhos
Summary: The production of doubled haploids is a biotechnological approach that accelerates the development of parental lines in breeding programs. Traditional inbreeding methods require 5 to 6 generations to achieve sufficient homozygosity, while haploid technologies can reduce this to 2 generations. Gynogenesis is the most common method for producing doubled haploids in sugar and red beets, with success seen especially in sugar beets.
VAVILOVSKII ZHURNAL GENETIKI I SELEKTSII
(2021)
Article
Plant Sciences
Samira Tajedini, Baratali Fakheri, Mohsen Niazian, Nafiseh Mahdinezhad, Abdelbagi Mukhtar Ali Ghanim, Ali Kazemi Pour, Ivan Ingelbrecht, Mehran E. Shariatpanahi
Summary: Two different androgenesis pathways, shed-microspore culture (SMC) and anther culture (AC), were developed for haploid induction in mutagenized M-1 rice cultivars. The effects of various factors on induction and regeneration phases were evaluated. The SMC method showed better results in terms of the number of regenerated embryos. Chromosome counting and flow cytometry analysis confirmed the presence of haploid plants, providing a valuable foundation for rice breeding research.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Chuanhong Liu, Gengxing Song, Bing Fang, Zhiyong Liu, Jiaqi Zou, Shiyao Dong, Sai Du, Jie Ren, Hui Feng
Summary: Isolated Microspore Culture (IMC) is an efficient method for obtaining homozygous strains, but it is challenging in ornamental kale. Histone acetylation, an important epigenetic mechanism, can promote microspore embryogenesis. The study found that the histone deacetylase inhibitor SAHA can enhance embryogenesis in microspores of cut-flower ornamental kale.
Article
Biochemistry & Molecular Biology
Dongdong Li, Haoying Wang, Meng Wang, Guoliang Li, Zhe Chen, Willmar L. Leiser, Thea Mi Weiss, Xiaohuan Lu, Ming Wang, Shaojiang Chen, Fanjun Chen, Lixing Yuan, Tobias Wuerschum, Wenxin Liu
Summary: The study analyzed the biomass, grain yield, and phosphorus use efficiency (PUE)-related traits of 359 maize inbred lines grown under low-P and normal-P conditions. It identified candidate genes for various traits under different phosphorus conditions and characterized key genes related to phosphate transporters or stress responses. The research provides insights into the genetic dissection and molecular characterization of PUE in maize, highlighting the importance of selecting genotypes with high phosphorus use efficiency.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Plant Sciences
Xiujuan Yang, Matthew R. Tucker
Summary: The plant ovule is a crucial organ that directly leads to seed formation, with its number and flower fertility being significant factors influencing yield. Studies have shown challenges in increasing one without compromising the other. Recent findings highlight regulatory pathways in Arabidopsis and cereal crops that could help overcome this yield constraint by targeting hormones and transcriptional regulators in the optimization of reproductive traits.
CURRENT OPINION IN PLANT BIOLOGY
(2021)
Article
Plant Sciences
Brendan M. Kupke, Matthew R. Tucker, Jason A. Able, Kenton D. Porker
Summary: Matching flowering time to the optimal period is crucial for maximizing yield in Mediterranean cropping zones. Plant hormonal products, particularly gibberellin (GA), have shown potential for altering development and delaying flowering in barley. Trinexapac-ethyl, a GA inhibitor, was able to delay flowering and extend pre-anthesis phases of development in genetically diverse barley cultivars.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Haoyu Lou, Matthew R. Tucker, Neil J. Shirley, Jelle Lahnstein, Xiujuan Yang, Chao Ma, Julian Schwerdt, Riccardo Fusi, Rachel A. Burton, Leah R. Band, Malcolm J. Bennett, Vincent Bulone
Summary: This study reveals the key role of HvCslF3 in barley root development, specifically in the elongation zone, by regulating the level of (1,4)-β-linked glucoxylan. Heterologous expression of HvCslF3 in Arabidopsis mutants complements the mutant phenotype and alters epidermal cell fate, suggesting similar functions of CslD and CslF gene families in root growth regulation.
Article
Multidisciplinary Sciences
Zhen Yuan, Hongliang Yang, Leiwen Pan, Wenhui Zhao, Lunping Liang, Anicet Gatera, Matthew R. Tucker, Dawei Xu
Summary: This study identified 116 HvBAHD acyltransferases from the barley genome and classified them into different groups. The absence of Agmatine Coumarol Transferase (ACT), associated with resistance to Gibberella fungi, in Arabidopsis was found. The HvBAHD genes were found to be expressed in various tissues and developmental stages, with high expression in seedlings.
SCIENTIFIC REPORTS
(2022)
Article
Agronomy
Leon Hinrich Oehme, Alice-Jacqueline Reineke, Thea Mi Weiss, Tobias Wuerschum, Xiongkui He, Joachim Mueller
Summary: This study utilized digital surface models created from RGB-images captured by a UAV to estimate the plant height of 400 maize genotypes. The results showed that the UAV-based estimation was most accurate between 39 to 68 days after sowing.
Article
Multidisciplinary Sciences
Riccardo Fusi, Serena Rosignoli, Haoyu Lou, Giuseppe Sangiorgi, Riccardo Bovina, Jacob K. Pattem, Aditi N. Borkar, Marco Lombardi, Cristian Forestan, Sara G. Milner, Jayne L. Davis, Aneesh Lale, Gwendolyn K. Kirschner, Ranjan Swarup, Alberto Tassinari, Bipin K. Pandey, Larry M. York, Brian S. Atkinson, Craig J. Sturrock, Sacha J. Mooney, Frank Hochholdinger, Matthew R. Tucker, Axel Himmelbach, Nils Stein, Martin Mascher, Kerstin A. Nagel, Laura De Gara, James Simmonds, Cristobal Uauy, Roberto Tuberosa, Jonathan P. Lynch, Gleb E. Yakubov, Malcolm J. Bennet, Rahul Bhosale, Silvio Salvi
Summary: Root angle in crops is an important trait for efficient utilization of soil resources. This study identifies a regulatory gene called EGT1 that controls root angle by affecting cell wall stiffness in elongating root cortical tissue. Mutations in the EGT1 gene result in a striking root phenotype with steeper growth angles in barley and wheat. These findings suggest that EGT1 plays a crucial role in exerting an antigravitropic mechanism to control root angle in cereal crops.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Guillermo Garcia-Gimenez, Miriam Schreiber, George Dimitroff, Alan Little, Rohan Singh, Geoffrey B. Fincher, Rachel A. Burton, Robbie Waugh, Matthew R. Tucker, Kelly Houston
Summary: The transcriptional regulation of HvCslF6, the main gene contributing to (1,3;1,4)-beta-glucan biosynthesis in barley grain, was investigated. In-silico analysis of the putative promoter revealed over-representation of TF classes AP2/ERF, MYB, and bHLH. Functional characterization using a barley protoplast transient expression system identified HvMYB61 and HvMYB46/83 as putative activators of HvCslF6 expression.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Dongdong Li, Guoliang Li, Haoying Wang, Yuhang Guo, Meng Wang, Xiaohuan Lu, Zhiheng Luo, Xintian Zhu, Thea Mi Weiss, Sandra Roller, Shaojiang Chen, Lixing Yuan, Tobias Wuerschum, Wenxin Liu
Summary: Genotype-by-environment interaction (G-by-E) is a common and potentially problematic phenomenon in plant breeding. This study investigated the genotypic performance and plasticity of maize lines under different environments, focusing on soil phosphorus levels. The study identified several QTL associated with genotype performance and plasticity, as well as genes related to phosphorus use efficiency. The study also observed the superiority of G-by-E in genomic selection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Xiujuan Yang, Laura G. Wilkinson, Matthew K. Aubert, Kelly Houston, Neil J. Shirley, Matthew R. Tucker
Summary: Research has found that the cell wall composition of the ovule in barley affects grain size. The ovule in barley contains two types of cells with different cell wall pectin structures, which influence grain growth.
Article
Biochemistry & Molecular Biology
Main Ern Ang, James M. Cowley, Kuok Yap, Michael G. Hahn, Deirdre Mikkelsen, Matthew R. Tucker, Barbara A. Williams, Rachel A. Burton
Summary: Upon wetting, chia nutlets produce a gel-like capsule of mucilage, which is a significant part of their dietary fibre content. The composition and structure of chia nutlet mucilage are complex and contain several types of polymers. The fermentation of chia nutlet mucilage is poor, with only oligosaccharides being fermentable substrates. Grinding chia nutlets improves the fermentation and bioaccessibility of key nutrients, including dietary fibre.
Article
Plant Sciences
Cindy Callens, Jose Fernandez-Gomez, Matthew R. Tucker, Dabing Zhang, Zoe A. Wilson
Summary: This study investigated the effects of high temperature on three European spring barley varieties and found that increased temperature severely impacted male reproductive organs while female reproductive organs were less susceptible. The timing of heat stress applied during floral development had a significant impact on fertility, and different cultivars showed varying sensitivities to high temperature.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Biology
Jack H. Kelly, Matthew R. Tucker, Philip B. Brewer
Summary: Plants have the ability to sense their environment and adjust their growth accordingly, which is crucial for their survival. Strigolactones (SLs) are a type of plant hormone that plays a critical role in regulating lateral branching. When the growing conditions are poor, the production of SL increases, leading to a reduction in the number of branches a plant can produce. However, this response can also decrease crop yield. It has been discovered that natural variations in the SL hormone pathway have contributed to increased yields in staple crops like rice and maize. New technologies can be used to harness this knowledge and develop crop varieties with different SL functions, which can help improve crop architecture and yield in various environments.
Article
Plant Sciences
Caterina Selva, Xiujuan Yang, Neil J. Shirley, Ryan Whitford, Ute Baumann, Matthew R. Tucker
Summary: Analysis of barley multiovary mutants mov2.g and mov1 reveals the roles of HvSL1 and HvMADS16 genes in the development of cereal florets. The study identifies the genetic determinants and conservation in stamen formation pathways among cereals while also highlighting species-specific differences. The findings provide insights into floral architecture in Triticeae and contribute to crop improvement efforts.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Chaoqun Shen, Yueya Zhang, Gang Li, Jin Shi, Duoxiang Wang, Wanwan Zhu, Xiujuan Yang, Ludovico Dreni, Matthew R. Tucker, Dabing Zhang
Summary: Temperature is an important factor affecting plant growth and diversity. This study reveals a previously unknown mechanism of plant floral organ development and temperature response. The MADS8 gene plays a conserved role in maintaining pistil number and ovule initiation in barley and rice, and its activity is temperature-dependent. This study also uncovers the regulatory relationship between MADS8 and other genes.
