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Plant Sciences
Kaiwei Liu, Keun Pyo Lee, Jianli Duan, Eun Yu Kim, Rahul Mohan Singh, Minghui Di, Zhuoling Meng, Chanhong Kim
Summary: In this study, researchers identified AtRsmD and AtRimM as important factors involved in the 16S rRNA maturation in chloroplasts. AtRsmD catalyzes the m(2)G915 modification of 16S rRNA, while AtRimM is involved in ribosome assembly. These two factors interact with each other and cooperatively regulate the maturation of 16S rRNA and ribosome assembly, contributing to chloroplast proteostasis.
Article
Plant Sciences
Shujing Chen, Xinhuang Zeng, Yiqi Li, Shijun Qiu, Xiaoqun Peng, Xinjue Xie, Yujie Liu, Chancan Liao, Xiaoyan Tang, Jianxin Wu
Summary: This study investigated the functions of plant plastid ribosomal protein L18s (PRPL18s) and found that they play critical roles in plastid ribosome biogenesis, plastid intron splicing, and chloroplast development, and are essential for plant survival.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Le Nguyen Tieu Ngoc, Su Jung Park, Jing Cai, Trinh Thi Huong, Kwanuk Lee, Hunseung Kang
Summary: Ribosomal RNA (rRNA) methylation is crucial for ribosome assembly and function, which are important for plant growth and stress responses. In this study, a new rRNA methyltransferase, RsmD, was identified in Arabidopsis chloroplasts, and it plays a role in plant adaptation to cold stress.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Li Zhang, Jingli Chen, Liqun Zhang, Ying Wei, Yajuan Li, Xinyun Xu, Hui Wu, Zhong-Nan Yang, Jirong Huang, Fenhong Hu, Weihua Huang, Yong-Lan Cui
Summary: The plastid-localized PPR protein EMB1270 is essential for chloroplast biogenesis in Arabidopsis thaliana, playing a crucial role in the accumulation of photosynthetic proteins and splicing efficiency of specific plastid introns. EMB1270 interacts with CFM2 to facilitate the splicing of group II introns, highlighting its importance in coordinating chloroplast and nuclear gene expression.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Article
Plant Sciences
A. A. Andreeva, I. A. Bychkov, N. V. Kudryakova, V. V. Kusnetsov
Summary: The role of PTF1/TCP13 in regulating the expression of the psbD plastid gene is controversial. Overexpression of PTF1/TCP13 did not increase psbD transcript accumulation, but it was upregulated by light or ABA exposure. PTF1/TCP13 acted as a negative regulator of salt stress and was involved in the ABA-dependent regulatory chain for modifying gene expression.
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Ian Small, Joanna Melonek, Alexandra-Viola Bohne, Joerg Nickelsen, Christian Schmitz-Linneweber
Summary: Plant organellar RNA metabolism is regulated by numerous nucleus-encoded RNA-binding proteins (RBPs) that control RNA stability, processing, and degradation. These post-transcriptional processes are critical for the production of essential components in chloroplasts and mitochondria, which are necessary for organellar biogenesis and plant survival. While many organellar RBPs have been identified, our understanding of their mechanisms and kinetics in fulfilling their functions remains incomplete. This review provides a comprehensive summary of plant organellar RNA metabolism, focusing on the mechanistic aspects and kinetics of RBP functions.
Article
Biochemistry & Molecular Biology
Aude Trinquier, Jonathan E. Ulmer, Laetitia Gilet, Sabine Figaro, Philippe Hammann, Lauriane Kuhn, Frederique Braun, Ciaran Condon
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Biochemistry & Molecular Biology
Benoit Castandet, Arnaud Germain, Amber M. Hotto, David B. Stern
NUCLEIC ACIDS RESEARCH
(2019)
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Biochemistry & Molecular Biology
Michaela Sikova, Jana Wiedermannova, Martin Prevorovsky, Ivan Barvik, Petra Sudzinova, Olga Kofronova, Oldrich Benada, Hana Sanderova, Ciaran Condon, Libor Krasny
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Biotechnology & Applied Microbiology
Coralie E. Salesse-Smith, Robert E. Sharwood, Florian A. Busch, David B. Stern
PLANT BIOTECHNOLOGY JOURNAL
(2020)
Review
Biochemistry & Molecular Biology
Aude Trinquier, Sylvain Durand, Frederique Braun, Ciaran Condon
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2020)
Article
Cell Biology
Dmitriy Ignatov, Karolis Vaitkevicius, Sylvain Durand, Laty Cahoon, Stefanie S. Sandberg, Xijia Liu, Birgitte H. Kallipolitis, Patrik Ryden, Nancy Freitag, Ciaran Condon, Jorgen Johansson
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Multidisciplinary Sciences
Yiming Cai, Ben Usher, Claude Gutierrez, Anastasia Tolcan, Moise Mansour, Peter C. Fineran, Ciaran Condon, Olivier Neyrolles, Pierre Genevaux, Tim R. Blower
Article
Biochemistry & Molecular Biology
Stephanie Oerum, Tom Dendooven, Marjorie Catala, Laetitia Gilet, Clement Degut, Aude Trinquier, Maxime Bourguet, Pierre Barraud, Sarah Cianferani, Ben F. Luisi, Ciaran Condon, Carine Tisne
Article
Plant Sciences
Leila Feiz, Susan R. Strickler, Joyce van Eck, Linyong Mao, Navid Movahed, Caroline Taylor, Poornima Gourabathini, Zhangjun Fei, David B. Stern
Article
Plant Sciences
Leila Feiz, Yukari Asakura, Linyong Mao, Susan R. Strickler, Zhangjun Fei, Margarita Rojas, Alice Barkan, David B. Stern
Summary: A new study shows that CRM Family Member1 (CFM1) facilitates the splicing of chloroplast introns in plants, particularly those not previously known to require CRM domain proteins. Deficiencies in these introns result in compromised chloroplast protein synthesis, leading to whole-plant chlorotic phenotypes.
Article
Biochemistry & Molecular Biology
Stephanie Oerum, Marjorie Catala, Maxime Bourguet, Laetitia Gilet, Pierre Barraud, Sarah Cianferani, Ciaran Condon, Carine Tisne
Summary: The NRase M5 enzyme plays a crucial role in the maturation of 5S rRNA in low G + C Gram-positive bacteria, by forming a complex with ribosomal protein uL18. Structural data reveals the accommodation of two Mg2+ ions in the active site pocket of the catalytic Toprim domain of M5 and their importance for catalysis. Solution studies support the proposed 3'-5' order of removal of pre-5S rRNA extensions and show the corresponding M5 structural rearrangements during catalysis.
Article
Plant Sciences
Amber M. Hotto, Coralie Salesse-Smith, Myat Lin, Florian A. Busch, Isabelle Simpson, David B. Stern
Summary: This study identified a minimal set of Rubisco assembly factors in plants, which may contribute to regulating the C-4 pathway.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Biochemistry & Molecular Biology
Sebastien Fleurier, Tanja Dapa, Olivier Tenaillon, Ciaran Condon, Ivan Matic
Summary: Regulating ribosome synthesis is crucial for cells to adapt to changing environments. Multiple ribosomal RNA (rRNA) operons in bacteria serve to prevent RNA polymerase overload, which can lead to chromosome replication failure and cell death. Reduced ribosome synthesis capacity results in longer transition time from stationary phase to growth, with higher mortality rates.
NUCLEIC ACIDS RESEARCH
(2022)
Biographical-Item
Biochemistry & Molecular Biology
Isabelle Iost, Ciaran Condon
MOLECULAR MICROBIOLOGY
(2023)
Article
Biology
Ciaran Condon, Olivier Pellegrini, Laetitia Gilet, Sylvain Durand, Frederique Braun
Summary: Most bacterial ribonucleases come from either Escherichia coli or Bacillus subtilis, which are phylogenetically distant from each other and have distinct RNA maturation and degradation machineries. The evolutionary space between these two bacteria is characterized by opposing gradients of enzymes that fade out and fade in as one moves across the phylogenetic tree from E. coli to B. subtilis.
COMPTES RENDUS BIOLOGIES
(2021)