Review
Plant Sciences
Angel Llamas, Esperanza Leon-Miranda, Manuel Tejada-Jimenez
Summary: Microalgae and diazotrophic organisms have wide-ranging biotechnological applications and can be used in biofuel production, agriculture, high-value-added product manufacturing, wastewater decontamination, and carbon sequestration. The symbiotic relationship between microalgae and diazotrophic organisms can be artificially created and has the potential to reduce production costs, increase biomass yields, and improve biotechnological processes.
Article
Biotechnology & Applied Microbiology
Liufu Wang, Di Ming, Yingying pan, Liqiu Shi, Wenzhi Wei
Summary: The main aim of this study is to investigate the effects of combined nitrogen limitation and hydrogen peroxide treatment on lipid accumulation in Chlorella sorokiniana. In addition, the relationships between reactive oxygen species (ROS), Ca2+ and lipogenic genes were analyzed to study the regulative networks involved in lipid accumulation. The results showed that lipid content and levels of endogenous ROS, Ca2+ increased under nitrogen limitation with H2O2 added, and ROS level could affect lipid accumulation through lipogenic gene expression. The study provides an effective strategy to improve lipid accumulation in C. sorokiniana and offers evidence of the involvement of ROS, Ca2+ and lipogenic genes in lipid biosynthesis in microalgae under combined treatment.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Microbiology
Tan Liu, Zhihui Chen, Yunhua Xiao, Mingmin Yuan, Chenkai Zhou, Gang Liu, Jun Fang, Bo Yang
Summary: Nitrogen limitation and starvation can increase the lipid content of microalgal cells but decrease biomass productivity, pigment concentration, and protein content. Three-day nitrogen starvation is a suitable strategy to produce lipid-rich biomass and causes changes in cell morphology. Saturated fatty acids dominate, making microalgae a potential feedstock for biodiesel production.
Article
Mathematics
Pablo Otalora, Jose Luis Guzman, Manuel Berenguel, Francisco Gabriel Acien
Summary: The industrial production of microalgae is a sustainable and interesting process, especially in terms of its applications in wastewater treatment. Neural network models have been developed to optimize the process and characterize the pH dynamics in different raceway reactors. These models are able to predict pH profiles using available measurable process data and demonstrate the potential of artificial neural networks in modeling continuous dynamic systems in the industry.
Review
Biochemistry & Molecular Biology
Assunta Saide, Kevin A. Martinez, Adrianna Ianora, Chiara Lauritano
Summary: Microalgae can produce compounds with allelopathic, antimicrobial, antioxidant, anti-inflammatory, anticancer, and other beneficial biological activities, which have potential for human health applications in pharmaceutical and other industrial fields. Further research is needed to fully exploit their potential.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Eleonora Montuori, Anita Capalbo, Chiara Lauritano
Summary: This review provides an overview of marine compounds with potential applications in the treatment and prevention of melanoma, including the compound Marizomib currently in clinical trials. Further clinical trials are needed to explore the potential of these promising marine molecules.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
J. K. Penhaul Smith, A. D. Hughes, L. McEvoy, J. G. Day
Summary: Two previously identified methods of contaminant removal from crude glycerol were tested for mixotrophic cultivation of Phaeodactylum tricornutum. pH adjusted crude glycerol showed comparable cell densities, growth rates, and fatty acid profiles to reagent grade glycerol. This method of contaminant removal may reduce the costs of microalgal culture.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Engineering, Chemical
Hao-Cheng Yu, Chyi-How Lay, Peer Mohamed Abdul, Jane-Yii Wu
Summary: This study optimized the cultivation conditions for a local microalgae species, Chlorella sp. G3H3-1-2, with high pH-resistant, high growth rate, and high lipid content. It was found that a pH range of 6.0 to 8.0 with glucose as the carbon source resulted in the highest specific growth rate of 1.22 day(-1) for Chlorella sp. G3H3-1-2. However, the highest total FAMEs content of 59% in the biomass of 1.75 g/L was obtained when using the combination of glucose and (NH4)2SO4 at a high pH value of 10.
Article
Chemistry, Medicinal
Rafael Carrasco-Reinado, Maria Bermudez-Sauco, Almudena Escobar-Nino, Jesus M. Cantoral, Francisco Javier Fernandez-Acero
Summary: This review highlights the importance of microalgae as primary producers and the significance of utilizing omics approaches in microalgae research. Proteomics, as a powerful technique, is discussed in particular for its ability to identify proteins with high commercial value.
Article
Chemistry, Medicinal
Renate Doving Osvik, Richard Andre Ingebrigtsen, Maria Fredrika Norrbin, Jeanette Hammer Andersen, Hans Christian Eilertsen, Espen Holst Hansen
Summary: The grazing pressure on the marine diatom Porosira glacialis can affect its metabolome and result in changes in bioactivity. Cocultivation with zooplankton increases the bioactivity of P. glacialis, but has no noticeable effect on the activity against biofilm formation and bacterial growth.
Review
Environmental Sciences
Ayusmita Ray, Manoranjan Nayak, Amit Ghosh
Summary: Microalgae-based biofuels are recognized globally as environmentally friendly and economically viable options, with the ability to extract high value-added compounds. Co-cultivation practices help enhance biomass productivity and commercialization, contributing to the advancement of microalgal fuel technologies.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Review
Biotechnology & Applied Microbiology
Angelo Del Mondo, Arianna Smerilli, Luca Ambrosino, Adriana Albini, Douglas M. Noonan, Clementina Sansone, Christophe Brunet
Summary: Microalgae are photosynthetic organisms with high levels of biological and functional diversities, being recognized as promising sources for bioactive metabolites such as phenolic compounds. Despite being less investigated, microalgal phenolic compounds are considered an important natural source of bioactive molecules.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Elen Saturnino Ferreira, Flavio Ferreira da Silva Binotti, Edilson Costa, Eduardo Pradi Vendruscolo, Eliana Duarte Cardoso Binotti, Josiane Souza Salles, Jussara Souza Salles
Summary: This study evaluated the effects of B3 vitamins on the growth and CO2 fixation of Chlorella vulgaris. The results showed that vitamin supplementation promoted growth and improved the physicochemical properties of the culture medium, but the effect was short-lived.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Multidisciplinary Sciences
Jilong Han, Tingting Guo, Yaojing Yue, Zengkui Lu, Jianbin Liu, Chao Yuan, Chune Niu, Min Yang, Bohui Yang
Summary: Tail adipose tissue plays a crucial role in enhancing environmental tolerance for sheep, and this study aimed to explore the development mechanisms of this trait. Proteomic analysis revealed differentially expressed proteins in the tail adipose tissues between fat-tailed and thin-tailed sheep, mainly related to metabolism pathways and the peroxisome proliferator activated receptor signaling pathway. Up-regulated proteins like ACS L1, HSD17 beta 4, FABP4 may contribute to tail fat deposition by promoting adipocyte proliferation and fat accumulation.
Article
Plant Sciences
Rui Wang, Xiaoling Miao
Summary: This study uncovers the effect of nitrogen deprivation on fatty acid desaturation and its regulatory mechanism. Early nitrogen deprivation reduces fatty acid desaturation without increasing lipid content. Lipidomic analysis reveals the transfer of unsaturated fatty acids from phospholipids and glycolipids to triacylglycerols. Functional characterization identifies certain transcription factors playing important roles in promoting total fatty acid accumulation and reducing fatty acid desaturation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Marc-Andre Cormier, Jean-Baptiste Berard, Gael Bougaran, Clive N. Trueman, Daniel J. Mayor, Richard S. Lampitt, Nicholas J. Kruger, Kevin J. Flynn, Rosalind E. M. Rickaby
Summary: The traditional separation between primary producers and consumers in the marine food web is being replaced by the paradigm of mixoplankton, which are planktonic protists capable of both heterotrophy and autotrophy. This shift in understanding has implications for primary production, biomass transfer, and CO2 sequestration in the deep ocean. However, there is currently no tool to quantify the relative contributions of autotrophy and heterotrophy in planktonic protists, hindering our understanding of carbon cycling in marine ecosystems. This study explores the use of hydrogen isotopic signature of lipid biomarkers as a novel tool to investigate the prevalence of mixotrophy in marine microorganisms.
Article
Plant Sciences
Anne Pajot, Johann Lavaud, Gregory Carrier, Matthieu Garnier, Bruno Saint-Jean, Noemie Rabilloud, Caroline Baroukh, Jean-Baptiste Berard, Olivier Bernard, Luc Marchal, Elodie Nicolau
Summary: Differences in lhc classification in Chromista were observed. A classification of the lhcf family with specific groups for haptophytes, diatoms, and seaweeds was proposed. Similarity analysis was performed to identify and characterize Fucoxanthin and Chlorophyll a/c-binding Protein (FCP) in the haptophyte microalgae Tisochrysis lutea. The study revealed dynamic changes in lhc gene expression with nitrogen depletion and the exclusive nighttime expression of the lhcx2 gene in T. lutea, suggesting its role in protecting cells from light exposure after prolonged darkness.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Chemistry, Medicinal
Anne Pajot, Gia Hao Huynh, Laurent Picot, Luc Marchal, Elodie Nicolau
Summary: This review presents the biological functions, chemical structure, and functional activities of fucoxanthin in human health. The production methods and extraction technologies are discussed, along with an analysis of the market and future prospects.
Article
Biotechnology & Applied Microbiology
H. Bonnefond, Y. Lie, T. Lacour, B. Saint-Jean, G. Carrier, E. Pruvost, A. Talec, O. Bernard, A. Sciandra
Summary: Species domestication and improvement are important for increasing agricultural production. The microalgae industry can follow the same path by selecting and improving microalgal strains. The use of competition in a dynamic environment can drive the evolution and emergence of new adapted populations with enhanced properties. By selecting microalgae strains under variable light intensity, we successfully increased biomass productivity and observed the appearance of new alleles.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Review
Chemistry, Medicinal
Christiane Adrielly Alves Ferraz, Raphael Grougnet, Elodie Nicolau, Laurent Picot, Raimundo Goncalves de Oliveira Junior
Summary: Microalgal carotenoids have shown potential as adjuvant therapy for melanoma by inhibiting cell proliferation, migration, and invasion, while also enhancing the sensitivity of melanoma cells to conventional chemotherapy.
Article
Biotechnology & Applied Microbiology
Anne Pajot, Johann Lavaud, Gregory Carrier, Thomas Lacour, Luc Marchal, Elodie Nicolau
Summary: This study investigated the photoprotection mechanisms in two clonal strains of T. lutea with different basal pigment compositions. It was found that the two strains had different strategies for dissipating excess light energy and synthesizing photoprotective pigments. The expression of specific genes involved in the xanthophyll pigment cycles was also correlated with these diverging phenotypes.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Multidisciplinary Sciences
Jeremy Berthelier, Bruno Saint-Jean, Nathalie Casse, Gael Bougaran, Gregory Carrier
Summary: Microalgal domestication is an expanding research field that aims to maximize the potential of microalgae for biotechnological purposes. In this study, we investigated the stability and genetic changes of a domesticated strain of Tisochrysis lutea, which showed improved lipid traits compared to the native strain. We found specific genetic variations between the two strains and highlighted the role of transposable elements in the domesticated strain's improved phenotype.
Article
Biotechnology & Applied Microbiology
Judith Rumin, Gregory Carrier, Catherine Rouxel, Aurelie Charrier, Virginie Raimbault, Jean-Paul Cadoret, Gael Bougaran, Bruno Saint-Jean
Summary: This study evaluates the impact of random mutagenesis on the genetic diversity of populations of the diatom Phaeodactylum tricornutum. The results show that out of the five populations obtained following EMS treatment, only one had high genetic diversity.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Plant Sciences
Gregory Carrier, Jeremy Berthelier, Agathe Maupetit, Elodie Nicolau, Martial Marbouty, Nathalie Schreiber, Aurelie Charrier, Clemence Carcopino, Laura Leroi, Bruno Saint-Jean
Summary: This study investigated the intra-species diversity of the microalga Tisochrysis lutea and found genetic and phenotypic differences among different strains. The findings have important implications for the further development and utilization of this species.
EUROPEAN JOURNAL OF PHYCOLOGY
(2023)
Article
Biochemical Research Methods
Tanushree Halder, Elke Stroeher, Hui Liu, Yinglong Chen, Guijun Yan, Kadambot H. M. Siddique
Summary: Improving the root system of wheat is crucial for enhancing grain yield and climate resilience. This study identified proteins associated with root length and root mass in wheat using label-free quantitative proteomics. The findings revealed potential biomarkers that could be used for improving the root system of wheat.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Yinhua Pan, Quanqing Zou, Wu Yin, Zhen Huang, Yingzhu Zhao, Zongming Mo, Lihui Li, Jianrong Yang
Summary: A prognostic model of BC based on LNM-related genes was established in this study, providing guidance for prognosis evaluation and precise treatment of BC. The genes related to lymph node metastasis in BC are largely unknown and need further exploration. Searching for potential lymph node metastasis-related genes of BC will provide meaningful biomarkers for BC treatment. Our model, based on TCGA-BRCA data, effectively predicted patient outcomes and classified BC patients. The identified feature genes might have a predictive function in immunotherapy. The results of this study provide a new reference for the prognosis and treatment of BC patients with lymph node metastasis.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Qiushi Liang, Joon M. Pijnenburg, Erikjan Rijkers, Arnold G. Vulto, Ans T. van der Ploeg, Niek P. van Til, Eva C. Vlaar, Jeroen A. A. Demmers, W. W. M. Pim Pijnappel
Summary: Pompe disease is a lysosomal storage disorder characterized by glycogen accumulation in skeletal muscle, leading to profound pathology. This study showed that lentiviral gene therapy with LV-IGF2.GAAco can nearly completely correct disease-associated proteomic changes, supporting its future clinical development as a new treatment option for Pompe disease.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Chuang Liu, Wenjing Zhang, Qianli Dong, Haipeng Liu
Summary: This study fills the knowledge gap about the matrix proteins involved in the formation of mineralized exoskeletons in decapod crustaceans. By comparing with well-studied molluscan biomineralization proteins, it is proposed that decapod crustaceans evolved novel proteins while sharing some proteins with molluscs. This study provides insights into the evolution and adaptation of crustaceans and molluscs in forming their exoskeletons.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Rania D. Baka, Josipa Kules, Angelo Beletic, Vladimir Farkas, Dina Resetar Maslov, Blanka Beer Ljubic, Ivana Rubic, Vladimir Mrljak, Mark McLaughlin, David Eckersall, Zoe Polizopoulou
Summary: This study analyzed serum samples from dogs with epilepsy and identified potential markers of epilepsy, proteins involved in nerve tissue regeneration, and proteins altered by antiepileptic medication. The findings provide insight into the pathogenesis of epilepsy and the effects of medication on extracellular matrix proteins.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Viviane M. Codognoto, Fabiana F. de Souza, Thais R. Cataldi, Carlos A. Labate, Laiza S. de Camargo, Pedro H. Esteves Trindade, Roberto R. da Rosa Filho, Diego J. B. de Oliveira, Eunice Oba
Summary: This study aimed to compare urine proteomics from non- and pregnant buffaloes to identify potential biomarkers of early pregnancy. A total of 798 proteins were reported in the urine considering all groups, and the differential proteins play essential roles during pregnancy.
JOURNAL OF PROTEOMICS
(2024)