Article
Biochemistry & Molecular Biology
Kurtis F. Budden, Shaan L. Gellatly, Annalicia Vaughan, Nadia Amorim, Jay C. Horvat, Nicole G. Hansbro, David L. A. Wood, Philip Hugenholtz, Paul G. Dennis, Peter A. B. Wark, Philip M. Hansbro
Summary: In this study, it was found that regardless of its acetate-producing capacity, Bifidobacterium longum subsp. longum alleviated cigarette smoke-induced inflammation, reduced inflammatory cytokine expression and adhesion factor expression, and improved the depletion of cecal butyrate levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Applied
Mi-Ju Kim, So Won Shin, Hyeon-Be Kim, Eiseul Kim, Hae-Yeong Kim
Summary: As interest in probiotics grows, accurate description of probiotic compositions in commercial products is increasingly needed. Three specific LAMP primer sets for distinguishing B. longum subspecies were designed and optimized for on-site detection, successfully detecting target subspecies in probiotic products within approximately 45 minutes. These rapid detection methods are useful for identifying B. longum subspecies in probiotic products.
Review
Gastroenterology & Hepatology
Susan Mills, Bo Yang, Graeme J. Smith, Catherine Stanton, R. Paul Ross
Summary: The colonization of Bifidobacterium in the neonatal gut highlights its significance to human health. Bifidobacterium longum is the most abundant species during early stages, but its levels decrease with age and in certain diseases. Research has revealed that B. longum produces bioactive molecules and can impact immune responses in various parts of the body. This review emphasizes the biological and clinical impacts of B. longum throughout different stages of human life, suggesting the need for further research and clinical trials to explore its potential in treating and preventing diseases.
Article
Biotechnology & Applied Microbiology
Anna V. Begunova, Irina V. Rozhkova, Olga A. Glazunova, Konstantin V. Moiseenko, Olga S. Savinova, Tatyana V. Fedorova
Summary: This article presents new data on Bifidobacterium longum MC-42, a strain used in commercial dairy products in Russia for almost 40 years, demonstrating its high enzyme activities and inhibitory effects on pathogen growth. The absence of commonly transmittable antibiotic resistance genes in its genome makes it safe for use as a probiotic and starter culture. Additionally, the impact of growth-promoting additives on the fermentation profile of B. longum MC-42 was assessed, showing significant changes in enzyme activities and properties of the fermented products.
FERMENTATION-BASEL
(2021)
Article
Food Science & Technology
Kan Gao, Xueliang Ren, Cailing Chen, Qiuling Fan, Yanjun Li, Haifeng Wang, Su Chen
Summary: The probiotic strain Bifidobacterium longum WHH2270 exhibits hypoglycemic effects on type 2 diabetes by inhibiting α-glucosidase activity and reshaping the gut microbiome. It improves glucose tolerance and insulin resistance by increasing the abundance of beneficial bacteria and short-chain fatty acid production.
JOURNAL OF FOOD SCIENCE
(2023)
Article
Microbiology
Pedro A. A. Reyes-Castillo, Raquel Gonzalez-Vazquez, Edgar Torres-Maravilla, Jessica I. I. Bautista-Hernandez, Eduardo Zuniga-Leon, Martha Leyte-Lugo, Leovigildo Mateos-Sanchez, Felipe Mendoza-Perez, Maria Angelica Gutierrez-Nava, Diana Reyes-Pavon, Alejandro Azaola-Espinosa, Lino Mayorga-Reyes
Summary: This study aims to characterize the probiotic potential of B. longum LBUX23 isolated from neonates' feces based on its biochemical and genomic functionality. The results suggest that B. longum LBUX23 has the potential for antioxidant and anti-cholesterol effects, making it a candidate for further in vivo testing.
Article
Biochemistry & Molecular Biology
Jinchi Jiang, Caie Wu, Chengcheng Zhang, Qingsong Zhang, Leilei Yu, Jianxin Zhao, Hao Zhang, Arjan Narbad, Wei Chen, Qixiao Zhai
Summary: This study found that different strains of B. longum have strain-specific effects in alleviating hypercholesterolemia, mainly due to differences in bile salt deconjugation ability, cholesterol assimilation ability, expressions of key genes involved in cholesterol metabolism, and alterations of gut microbiota.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Applied
Zhikun Yang, Mingrui Li, Yanxiao Li, Xin Wang, Zhihua Li, Jiyong Shi, Xiaowei Huang, Xiaodong Zhai, Xiaobo Zou, Yunyun Gong, Melvin Holmes, Megan Povey, Jianbo Xiao
Summary: In this study, a gelatin/gellan gum-based bilayer emulsion film was developed to improve the survivability of Bifidobacterium longum during storage. Baobab seed oil was added to the gelatin matrix as the outer layer, while blueberry anthocyanin extract was incorporated into the gellan gum-based inner layer to enhance the viability of B. longum. The bilayer film showed successful entrapment of the probiotics and exhibited the highest survivability and viable cell numbers of B. longum during storage. The bilayer film also maintained satisfactory probiotic viability in steamed bread coating application.
Article
Microbiology
Zhiqian Bi, Enqing Cui, Yingying Yao, Xiaoyao Chang, Xiaoyang Wang, Yuhui Zhang, Gen-Xing Xu, Hongqin Zhuang, Zi-Chun Hua
Summary: This study demonstrates that using Bifidobacterium longum as a delivery system for endostatin can effectively alleviate symptoms of inflammatory bowel disease and colitis-associated cancer. The combination of endostatin and B. longum also reduces tumor formation and alters the gut microbiota, suggesting a promising therapeutic strategy.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Nutrition & Dietetics
Xinran Han, Fei Liu, Qiuxiang Zhang, Bingyong Mao, Xin Tang, Jie Huang, Renmei Guo, Jianxin Zhao, Hao Zhang, Shumao Cui, Wei Chen
Summary: The study found that Zn-enriched CCFM1195 is an effective method for zinc supplementation, which can better restore physical problems caused by zinc deficiency, especially at low doses. In addition, zinc supplementation also has a significant impact on the gut microbiota.
Article
Nanoscience & Nanotechnology
Fangfang Cao, Lulu Jin, Yong Gao, Yuan Ding, Hongyang Wen, Zhefeng Qian, Chenyin Zhang, Liangjie Hong, Huang Yang, Jiaojiao Zhang, Zongrui Tong, Weilin Wang, Xiaoyuan Chen, Zhengwei Mao
Summary: Combining artificial enzymes with probiotics, modified probiotics are used to treat inflammatory bowel disease, overcoming limitations of traditional treatments and showing promising outcomes.
NATURE NANOTECHNOLOGY
(2023)
Article
Nutrition & Dietetics
Shaoqi Shi, Qi Zhang, Yue Sang, Shaoyang Ge, Qi Wang, Ran Wang, Jingjing He
Summary: This study investigated the effect of Bifidobacterium longum BB68S on cognitive functions in healthy older adults without cognitive impairment. The results showed that BB68S significantly improved cognitive functions, especially in areas such as immediate memory, visuospatial/constructional ability, attention, and delayed memory. Additionally, BB68S also had a beneficial regulatory effect on the participants' gut microbiota by increasing the abundance of beneficial bacteria and decreasing the abundance of bacteria related to cognitive impairment. This study supports probiotics as a strategy to promote healthy aging and advance cognitive aging research.
Article
Biology
Dongxue Huo, Chaoping Cen, Haibo Chang, Qianying Ou, Shuaiming Jiang, Yonggui Pan, Kaining Chen, Jiachao Zhang
Summary: A 6-month study conducted by Huo et al in patients with Graves' Disease showed that the combination of probiotic Bifidobacterium longum with methimazole potentially improves thyroid function and regulates TRAb concentration through the gut-thyroid axis.
COMMUNICATIONS BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Monica F. Quintanilha, Vivian C. Miranda, Ramon O. Souza, Bruno Gallotti, Clenio Cruz, Elandia A. Santos, Jacqueline Alvarez-Leite, Luis C. L. Jesus, Vasco Azevedo, Luisa M. Trindade, Valbert N. Cardoso, Enio Ferreira, Barbara A. Carvalho, Pedro M. G. Soares, Angelica T. Vieira, Jacques R. Nicoli, Flaviano S. Martins
Summary: This study demonstrates that Bifidobacterium longum 5(1A), a probiotic, has a protective effect against intestinal damage caused by irinotecan, suggesting its potential use as a probiotic therapy for mucositis.
Article
Food Science & Technology
Olga V. Averina, Aleksey S. Kovtun, Dilara A. Mavletova, Rustam H. Ziganshin, Valery N. Danilenko, Dasha Mihaylova, Denica Blazheva, Aleksandar Slavchev, Mariya Brazkova, Salam A. Ibrahim, Albert Krastanov
Summary: This study provides insights into the mechanism of oxidative stress response in B. longum and factors contributing to its survival in functional food products through transcriptomic, proteomic, and metabolomic analyses.
Review
Biochemical Research Methods
Annelein Meisner, Beatrix Wepner, Tanja Kostic, Leo S. van Overbeek, Christine J. Bunthof, Rafael Soares Correa de Souza, Marta Olivares, Yolanda Sanz, Lene Lange, Doreen Fischer, Angela Sessitsch, Hauke Smidt
Summary: Microbiomes are essential players in various ecosystems and have significant impacts on biotechnological applications and health. However, current research mostly focuses on individual ecosystems, and a systems approach is needed to fully understand and utilize microbiomes for sustainable bioeconomy.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Editorial Material
Gastroenterology & Hepatology
Yolanda Sanz, Marta Olivares
Article
Nutrition & Dietetics
Rebeca Liebana-Garcia, Marta Olivares, Sonia M. Rodriguez-Ruano, Veronica Tolosa-Enguis, Isabel Chulia, Lidia Gil-Martinez, Enrique Guillamon, Alberto Banos, Yolanda Sanz
Summary: In this study, propyl propane thiosulfinate (PTS) showed dose-dependent anti-obesogenic effects in a murine model of diet-induced obesity. PTS prevented weight gain, adipocyte enlargement, inflammation, and gut barrier dysfunction caused by the obesogenic diet. The higher dose of PTS also improved glucose metabolism, liver homeostasis, lipid metabolism, and thermogenic capacity of brown adipose tissue.
Article
Gastroenterology & Hepatology
Alfonso Benitez-Paez, Annick Hartstra, Max Nieuwdorp, Yolanda Sanz
Summary: This study analyzed the changes in the human gut microbiota during a fecal microbiota transplantation (FMT) trial using nanopore technology. The results show that the engraftment of donors' microbiota is correlated with the improvement of metabolic health in recipients. Furthermore, the study revealed that assessing nucleotide frequencies can successfully explore the engraftment of donor bacterial strains. The findings indicate that factors such as baseline gut microbiota configuration, sex, and age of donors should be considered for successful FMT in humans.
Review
Biochemistry & Molecular Biology
Helena Marcos Pasero, Aurora Garcia Tejedor, Juan Antonio Gimenez-Bastida, Jose Moises Laparra Llopis
Summary: Alzheimer's disease is a inflammation-related cognitive decline, with the immune microenvironment playing a crucial role in its pathogenesis. Various peripheral stimuli are involved in the progression and severity of the disease. Targeting immune signals and cells is achievable, but more understanding of their contribution to the disease is needed for effective interventions. An integrative perspective can improve the health of AD patients.
Review
Food Science & Technology
V Rossini, V Tolosa-Enguis, C. Frances-Cuesta, Y. Sanz
Summary: SARS-CoV-2 primarily affects the respiratory system, but it also targets the gastrointestinal tract. Prolonged gut disorders in COVID-19 patients are associated with decreased richness and diversity of the gut microbiota, immune deregulation, and delayed viral clearance. The composition and function of the gut microbiome may play a role in the progression of COVID-19. Strategies to modulate the gut microbiome are being investigated for population stratification and management of COVID-19 infection.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Review
Nutrition & Dietetics
Ana Agusti, Femke Lamers, Maria Tamayo, Carlos Benito-Amat, Gara V. Molina-Mendoza, Brenda W. J. H. Penninx, Yolanda Sanz
Summary: Exposure to early life stress can significantly impact mental and physical health. The role of the intestinal microbiome in human health, particularly mental health, is becoming increasingly evident. This systematic review found a link between early life stress and the gut microbiome, but no consensus microbiome signatures were identified, highlighting the need for further studies with consistent stressors and validated measures to draw definitive conclusions.
Article
Gastroenterology & Hepatology
Rebeca Liebana-Garcia, Marta Olivares, Carlos Frances-Cuesta, Teresa Rubio, Valerio Rossini, Guillermo Quintas, Yolanda Sanz
Summary: Hypercaloric diets disrupt the microbiome and epithelial cell functions, affecting glucose metabolism. In obese mice, intestinal proinflammatory group 1 innate lymphoid cells (ILC1s) play a role in obesity and metabolic disruption. Depletion of ILC1s leads to an increase in ILC3-IL-22 pathway, resulting in changes in gut hormones, insulin levels, and adiposity. ILC1s also affect the abundance of Akkermansia muciniphila and Bilophila spp. Intestinal ILC1s are important in connecting immunity, microbiome, enteroendocrine system, and glucose metabolism and adiposity control.
Editorial Material
Microbiology
Alejandra Flor-Duro, Marta Olivares, Yolanda Sanz
Summary: Tracking the journey of inulin, a soluble dietary fibre, reveals that it can alleviate liver disease in mice by undergoing transformation by the gut microbiota.
NATURE MICROBIOLOGY
(2023)
Article
Food Science & Technology
Aurora Garcia Tejedor, Claudia Monika Haros, Jose Moises Laparra Llopis
Summary: This study investigated the effects of Chenopodium quinoa's ingredients on the hepatic profile of major lipids and the functional differentiation of monocyte-derived macrophages and innate lymphoid cells in mice on a high-fat diet. The results showed that feeding the mice with a low-molecular-weight protein fraction (LWPF) improved their ability to utilize fatty acids and reduced hepatic lipid accumulation, while feeding them the lipid fraction (qLF) had no effect on lipid accumulation. Therefore, Chenopodium quinoa's ingredients are advantageous for controlling hepatic lipotoxicity and promoting the expansion and functional differentiation of liver macrophages and innate lymphoid cells.
Article
Food Science & Technology
Marina Romani-Perez, Clara Bullich-Vilarrubias, Inmaculada Lopez-Almela, Yolanda Sanz
Summary: This study reveals sex-dependent differences in the regulation of energy metabolism by the sodium channel Nav1.8, and suggests that activation of these neurons can improve oral glucose tolerance in females and inhibit glucagon secretion induced by glucose.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Biochemical Research Methods
Tanushree Halder, Elke Stroeher, Hui Liu, Yinglong Chen, Guijun Yan, Kadambot H. M. Siddique
Summary: Improving the root system of wheat is crucial for enhancing grain yield and climate resilience. This study identified proteins associated with root length and root mass in wheat using label-free quantitative proteomics. The findings revealed potential biomarkers that could be used for improving the root system of wheat.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Yinhua Pan, Quanqing Zou, Wu Yin, Zhen Huang, Yingzhu Zhao, Zongming Mo, Lihui Li, Jianrong Yang
Summary: A prognostic model of BC based on LNM-related genes was established in this study, providing guidance for prognosis evaluation and precise treatment of BC. The genes related to lymph node metastasis in BC are largely unknown and need further exploration. Searching for potential lymph node metastasis-related genes of BC will provide meaningful biomarkers for BC treatment. Our model, based on TCGA-BRCA data, effectively predicted patient outcomes and classified BC patients. The identified feature genes might have a predictive function in immunotherapy. The results of this study provide a new reference for the prognosis and treatment of BC patients with lymph node metastasis.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Qiushi Liang, Joon M. Pijnenburg, Erikjan Rijkers, Arnold G. Vulto, Ans T. van der Ploeg, Niek P. van Til, Eva C. Vlaar, Jeroen A. A. Demmers, W. W. M. Pim Pijnappel
Summary: Pompe disease is a lysosomal storage disorder characterized by glycogen accumulation in skeletal muscle, leading to profound pathology. This study showed that lentiviral gene therapy with LV-IGF2.GAAco can nearly completely correct disease-associated proteomic changes, supporting its future clinical development as a new treatment option for Pompe disease.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Chuang Liu, Wenjing Zhang, Qianli Dong, Haipeng Liu
Summary: This study fills the knowledge gap about the matrix proteins involved in the formation of mineralized exoskeletons in decapod crustaceans. By comparing with well-studied molluscan biomineralization proteins, it is proposed that decapod crustaceans evolved novel proteins while sharing some proteins with molluscs. This study provides insights into the evolution and adaptation of crustaceans and molluscs in forming their exoskeletons.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Rania D. Baka, Josipa Kules, Angelo Beletic, Vladimir Farkas, Dina Resetar Maslov, Blanka Beer Ljubic, Ivana Rubic, Vladimir Mrljak, Mark McLaughlin, David Eckersall, Zoe Polizopoulou
Summary: This study analyzed serum samples from dogs with epilepsy and identified potential markers of epilepsy, proteins involved in nerve tissue regeneration, and proteins altered by antiepileptic medication. The findings provide insight into the pathogenesis of epilepsy and the effects of medication on extracellular matrix proteins.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Viviane M. Codognoto, Fabiana F. de Souza, Thais R. Cataldi, Carlos A. Labate, Laiza S. de Camargo, Pedro H. Esteves Trindade, Roberto R. da Rosa Filho, Diego J. B. de Oliveira, Eunice Oba
Summary: This study aimed to compare urine proteomics from non- and pregnant buffaloes to identify potential biomarkers of early pregnancy. A total of 798 proteins were reported in the urine considering all groups, and the differential proteins play essential roles during pregnancy.
JOURNAL OF PROTEOMICS
(2024)