Article
Biochemistry & Molecular Biology
Fabrice Lejeune
Summary: Nonsense-mediated mRNA decay (NMD) is a mechanism for rapidly eliminating mRNAs with premature termination codons and also regulates multiple genes. Researchers have discovered that NMD must be regulated to express genes that are normally repressed by NMD under specific physiological conditions, so a comprehensive understanding of NMD regulation is important for therapeutic purposes.
Article
Biochemistry & Molecular Biology
Sarah E. Fritz, Soumya Ranganathan, Clara D. Wang, J. Robert Hogg
Summary: This study reveals that an alternative isoform of the core NMD factor UPF1, UPF1(LL), can remodel the specificity of NMD in response to cellular stress. UPF1(LL) is able to bypass specific protective RNA binding proteins, bind and down-regulate transcripts with long 3'UTRs, and induce NMD in response to cellular stress conditions.
Article
Clinical Neurology
Gabrielle Zuniga, Simon Levy, Paulino Ramirez, Jasmine De Mange, Elias Gonzalez, Maria Gamez, Bess Frost
Summary: This study investigates the mechanisms behind altered RNA processing in tauopathies, specifically focusing on the reduction of nonsense-mediated mRNA decay (NMD) activity. The researchers find that deficits in NMD contribute to neurodegeneration in tauopathy through aberrant RNA export and accumulation. They identify a pharmacological activator of NMD that suppresses neurodegeneration in a tau transgenic Drosophila model, suggesting potential therapeutic value for tauopathy patients.
ALZHEIMERS & DEMENTIA
(2023)
Review
Genetics & Heredity
Preeti Nagar, Md Rafikul Islam, Mohammad Alinoor Rahman
Summary: NMD is a mechanism that ensures gene expression accuracy and regulation by degrading erroneous transcripts and modulating the abundance of endogenous mRNAs. It plays diverse biological functions during development, adaptation, and stress response. In tumorigenesis, NMD can be exploited by tumor cells to degrade specific mRNAs or suppressed to promote the expression of oncoproteins.
Review
Virology
Md Robel Ahmed, Zhiyou Du
Summary: The interaction between viruses and hosts is dynamic and evolutionary. Eukaryotic hosts have multiple defense mechanisms against viral infection, including the nonsense-mediated mRNA decay (NMD) system. NMD ensures the accuracy of mRNA translation by degrading abnormal mRNAs. Many RNA viruses have internal stop codons (iTC), which activate NMD and lead to degradation of viral genomes. Some viruses are sensitive to NMD-mediated antiviral defense, while others have evolved mechanisms to overcome or escape NMD. This review summarizes the current understanding of NMD-mediated viral RNA degradation and the ways in which viruses compromise NMD for better infection.
Article
Biochemistry & Molecular Biology
Damaris Wallmeroth, Jan-Wilm Lackmann, Sabrina Kueckelmann, Janine Altmueller, Christoph Dieterich, Volker Boehm, Niels H. Gehring
Summary: The paralogous proteins UPF3A and UPF3B in humans play important roles in recognizing mRNAs targeted by nonsense-mediated mRNA decay (NMD). UPF3B supports NMD by bridging an exon junction complex (EJC) to the NMD factor UPF2. The role of UPF3A has been described as either a weak NMD activator or an NMD inhibitor. However, knockout or overexpression of UPF3A or knockout of UPF3B did not significantly affect global NMD activity. Co-depletion of UPF3A and UPF3B resulted in NMD inhibition, indicating functional redundancy between these two NMD factors.
Article
Biochemistry & Molecular Biology
Julie Carrard, Fiona Ratajczak, Josephine Elsens, Catherine Leroy, Rebekah Kong, Lucie Geoffroy, Arnaud Comte, Guy Fournet, Benoit Joseph, Xiubin Li, Sylvie Moebs-Sanchez, Fabrice Lejeune
Summary: The study has built a new screening system and identified two new molecules that can effectively inhibit nonsense-mediated mRNA decay (NMD). These molecules show no cellular toxicity at tested concentrations and have been validated in a lung cancer model with a nonsense mutation.
Review
Biochemistry & Molecular Biology
Lingling Sun, Justine Mailliot, Christiane Schaffitzel
Summary: Nonsense-mediated mRNA decay (NMD) is a cellular surveillance mechanism that degrades mRNAs with a premature stop codon and downregulates the expression of endogenous transcripts. The core NMD factors are conserved from yeast to human, but mammals have diversified NMD pathways with additional factors. This review summarizes the molecular mechanisms and cellular roles of NMD and discusses its implications in neurodevelopmental diseases, cancer, and strategies used by RNA viruses to evade recognition by the NMD machinery.
Article
Cell Biology
Alison J. Inglis, Alina Guna, Angel Galvez-Merchan, Akshaye Pal, Theodore K. Esantsi, Heather R. Keys, Evgeni M. Frenkel, Robert Oania, Jonathan S. Weissman, Rebecca M. Voorhees
Summary: Translation of mRNAs containing premature termination codons (PTCs) results in truncated protein products with deleterious effects. Nonsense-mediated decay (NMD) is responsible for detecting and degrading these proteins, and it relies on the ubiquitin proteasome pathway. Screening experiments identified factors involved in NMD-associated protein quality control and revealed a shared recognition event for both mRNA and protein branches of NMD.
JOURNAL OF CELL SCIENCE
(2023)
Article
Multidisciplinary Sciences
Hanae Sato, Robert H. Singer
Summary: The author developed a single-cell reporter system to investigate cell-to-cell variability of NMD efficiency. The study revealed a wide range of NMD efficiency in different cells, potentially linked to the expression levels of surveillance factors.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Yanjie Tan, Jing Zhang, Yi Jin
Summary: Skeletal muscle regeneration is a vital physiological process that responds to injury or disease. Nonsense-mediated mRNA decay (NMD) has been found to inhibit myoblast differentiation by targeting the phosphoinositide-3-kinase regulatory subunit 5 gene, leading to the suppression of MyoD's transcriptional activity. Inhibiting NMD can accelerate muscle regeneration, making it a potential therapeutic target for muscle-related injuries and diseases.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sebastian Grosse, Yen-Yun Lu, Ivo Coban, Bettina Neumann, Heike Krebber
Summary: This study revealed that Gbp2 and Hrb1 are involved in nonsense mediated decay of premature termination codon-containing mRNAs by forming a complex with Upf proteins. They aid in transmitting PTC recognition signals and promoting translation repression and RNA degradation, thus controlling mRNA quality beyond the nuclear border. Identification of SR proteins as global surveillance factors in yeast sheds light on their potential role in understanding the complex human system with diseases related to defects in SR proteins or NMD.
Article
Cell Biology
Giao Vu Quynh Tran, Jens Kleinehr, Hannah Franziska Preugschas, Darisuren Anhlan, Fakry Fahmy Mohamed, Christina Ehrhardt, Stephan Ludwig, Eike Roman Hrincius
Summary: Research has shown that nonsense-mediated mRNA decay (NMD) has restricting capacities against positive-sense single-stranded RNA viruses but limited impact on negative-sense single-stranded RNA viruses. While knockdown of NMD components slightly increased viral mRNA and protein levels, it did not significantly alter viral replication, suggesting that NMD may not effectively restrict (-)ssRNA viruses.
CELLULAR MICROBIOLOGY
(2021)
Review
Cell Biology
Kristoffer Leon, Melanie Ott
Summary: The Nonsense-mediated mRNA Decay (NMD) pathway is a conserved RNA quality control pathway in eukaryotic cells, playing roles in controlling viral pathogens and maintaining homeostasis.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Paul Jongseo Lee, Suzhou Yang, Yu Sun, Junjie U. Guo
Summary: Eukaryotes have evolved various mRNA surveillance mechanisms, with NMD functioning as a quality control mechanism and posttranscriptional gene regulation.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2021)
Article
Infectious Diseases
Estefania Abascal, Laura Perez-Lago, Miguel Martinez-Lirola, Alvaro Chiner-Oms, Marta Herranz, Imane Chaoui, Inaki Comas, My Driss El Messaoudi, Jose Antonio Garrido Cardenas, Sheila Santanton, Emilio Bouza, Dario Garcia-de-Viedma
Article
Environmental Sciences
Cristian Perez-Fernandez, Miguel Morales-Navas, Luis Manuel Aguilera-Saez, Ana Cristina Abreu, Laia Guardia-Escote, Ignacio Fernandez, Jose Antonio Garrido-Cardenas, Maria Teresa Colomina, Estela Gimenez, Fernando Sanchez-Santed
ENVIRONMENTAL RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Ana Molina-Marquez, Marta Vila, Rocio Rengel, Emilio Fernandez, Federico Garcia-Maroto, Javier Vigara, Rosa Leon
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Immunology
J. A. Garrido-Cardenas, C. de Lamo-Sevilla, M. T. Cabezas-Fernandez, F. Manzano-Agugliaro, M. Martinez-Lirola
Article
Environmental Sciences
Jose Antonio Garrido-Cardenas, Belen Esteban-Garcia, Ana Agueera, Jose Antonio Sanchez-Perez, Francisco Manzano-Agugliaro
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2020)
Article
Environmental Sciences
Esther Salmeron-Manzano, Jose Antonio Garrido-Cardenas, Francisco Manzano-Agugliaro
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2020)
Article
Environmental Sciences
Mila Cascajares, Alfredo Alcayde, Jose Antonio Garrido-Cardenas, Francisco Manzano-Agugliaro
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2020)
Article
Marine & Freshwater Biology
Nahla Bouzidi, Fatma Zili, Federico Garcia-Maroto, Diego Lopez Alonso, Hatem Ben Ouada
JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM
(2020)
Article
Agronomy
Concepcion M. Mesa-Valle, Jose A. Garrido-Cardenas, Jose Cebrian-Carmona, Miguel Talavera, Francisco Manzano-Agugliaro
Article
Genetics & Heredity
Lilia Gonzalez-Ceron, Jose Cebrian-Carmona, Concepcion M. Mesa-Valle, Federico Garcia-Maroto, Frida Santillan-Valenzuela, Jose Antonio Garrido-Cardenas
Summary: Analysis of genetic and amino acid diversity of pvcyrpa in Mexican and global parasites revealed nine private haplotypes in Southern Mexican sequences. These parasites showed high LD-R-2 index and low nucleotide diversity compared to isolates from South America or Asia. Diversity at pvcyrpa exon-1 is influenced by mutation and recombination, which is likely maintained by balancing selection due to selective immune pressure. Further study on the role of pvcyrpa in immune response and vaccine development is warranted.
Article
Public, Environmental & Occupational Health
Miguel Martinez-Lirola, Rana Jajou, Vanessa Mathys, Anandi Martin, Andrea Maurizio Cabibbe, Ana Valera, Pedro J. Sola-Campoy, Estefania Abascal, Sandra Rodriguez-Maus, Jose Antonio Garrido-Cardenas, Magdalena Bonillo, Alvaro Chiner-Oms, Begona Lopez, Silvia Vallejo-Godoy, Inaki Comas, Patricia Munoz, Daniela Maria Cirillo, Dick van Soolingen, Laura Perez-Lago, Dario Garcia de Viedma
Summary: Research on migrants from the Horn of Africa revealed that at least 15 cases of tuberculosis were diagnosed in Somalian and Eritrean migrants in four different European countries, suggesting potential en route transmission.
JOURNAL OF TRAVEL MEDICINE
(2021)
Article
Engineering, Chemical
Sara Turiel, Jose Antonio Garrido-Cardenas, Cintia Gomez-Serrano, Francisco Gabriel Acien, Lorenzo Carretero-Paulet, Saul Blanco
Summary: Tetradesmus almeriensis is a highly productive microalga known for producing bioactive compounds such as lutein, suitable for outdoor production in temperate and warm climates. It is classified as a Chlorophycean genus Tetradesmus, consistently clustering with other Scenedesmaceae species.
Article
Parasitology
Jose Antonio Garrido-Cardenas, Alfredo Alcayde, Francisco Manzano-Agugliaro, Concepcion M. Mesa-Valle
Summary: Parasitic diseases have been a major cause of death throughout history, affecting over a billion people worldwide. This study analyzed the relationship between articles published in the field of parasitology and international patents, revealing the dominance of malaria-related topics in the publications.
Article
Microbiology
Jose A. Garrido-Cardenas, Lilia Gonzalez-Ceron, Federico Garcia-Maroto, Jose Cebrian-Carmona, Francisco Manzano-Agugliaro, Concepcion M. Mesa-Valle
Summary: This study analyzed fifty years of severe malaria research worldwide, revealing the research trends, patterns, and progress made in the field. The results showed a steady increase in severe malaria publications, particularly in the last decade. Most of the publications are from USA and Europe, despite the disease occurring in Africa, South-East Asia, and the Americas. The study also identified the most frequent keywords, influential journals, and authors in the field.
