Article
Cell Biology
Binjie Yan, Yu Gui, Yanan Guo, Jiaxing Sun, Mahmoud Saifeddine, Jingti Deng, Joseph A. Hill, Morley D. Hollenberg, Zhi-Sheng Jiang, Xi-Long Zheng
Summary: This study found that (+)-JQ1 can directly inhibit smooth muscle contractility and regulate endothelial cell function by indirectly activating the PI3K/AKT/eNOS cascade. However, these effects appear to be unrelated to BRD4 inhibition.
Article
Medicine, Research & Experimental
Maria Grazia Zizzo, Adele Cicio, Maurizio Bruno, Rosa Serio
Summary: Evidence suggests that Prangoil and its components, 8-ocimene and carvacrol, can reduce spontaneous and KCl or oxytocin-induced contractions of rat myometrium by modulating calcium ion channels and intracellular calcium release. Further studies could contribute to evaluating the potential use of Prangoil in the treatment of uterine contractility disorders.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Multidisciplinary Sciences
Martin T. Johnson, Ping Xin, J. Cory Benson, Trayambak Pathak, Vonn Walter, Scott M. Emrich, Ryan E. Yoast, Xuexin Zhang, Gaoyuan Cao, Reynold A. Panettieri, Mohamed Trebak
Summary: This study reveals the up-regulation of STIM1 in the airway smooth muscle of asthmatic mice, which is involved in the metabolic and transcriptional reprogramming process of airway remodeling and the mechanism of airway hyperresponsiveness. STIM1 regulates the activation and secretion of airway smooth muscle cells, increases mitochondrial mass, oxidative phosphorylation, and glycolytic flux, and drives the development of asthma. It is proposed as a potential target for asthma therapy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cardiac & Cardiovascular Systems
Yen-Lin Chen, Zdravka Daneva, Maniselvan Kuppusamy, Matteo Ottolini, Thomas M. Baker, Eliska Klimentova, Soham A. Shah, Jennifer D. Sokolowski, Min S. Park, Swapnil K. Sonkusare
Summary: This study demonstrates the role of TRPV4(SMC) channels in blood pressure regulation, showing that their activity increases resting blood pressure in normal mice. The study also reveals the opposite effects of α1AR-TRPV4(SMC) signaling and intraluminal pressure-TRPV4(SMC)-BK channel signaling on blood pressure. In hypertension, constrictor α1AR-PKCα-TRPV4 signaling is upregulated, while dilator pressure-TRPV4-BK channel signaling is disrupted, leading to increased vasoconstriction and elevated blood pressure.
Review
Physiology
Susan Wray, Clodagh Prendergast, Sarah Arrowsmith
Summary: This study explores the mechanisms of calcium-activated chloride channels in smooth muscle tissues and the differences in their activation and regulation in different types of smooth muscles. The findings suggest that the expression and functional importance of CaCC vary in different smooth muscle tissues, with more complexity in activation and regulation observed in vascular smooth muscles.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Cell Biology
Jie Lin, Weiming Guo, Qingtian Luo, Qingping Zhang, Teng Wan, Changyu Jiang, Yuanchun Ye, Haihuan Lin, Gang Fan
Summary: This study demonstrates that pharmaceutical intervention targeting cellular senescence can alleviate age-related dysfunction of the caveolar Ca(V)3.2-RyR axis and potentially ameliorate age-associated cardiovascular disease.
Article
Physiology
Anne M. Roesler, Jovanka Ravix, Colleen M. Bartman, Brijeshkumar S. Patel, Marta Schiliro, Benjamin Roos, Lisa Nesbitt, Christina M. Pabelick, Richard J. Martin, Peter M. MacFarlane, Y. S. Prakash
Summary: Study found that high O2 affected CaSR expression in fetal lung ASM cells, leading to increased cellular calcium responses and stimulated intracellular and extracellular calcium ion responses, which have important implications for respiratory diseases such as asthma.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Cell Biology
Sou Inagaki, Yoshiaki Suzuki, Keisuke Kawasaki, Rubii Kondo, Yuji Imaizumi, Hisao Yamamura
Summary: In this study, the role of mitochondrial fusion protein 2 (Mfn2) in the coupling of sarcoplasmic reticulum (SR) and mitochondria in vascular smooth muscle cells (VSMCs) was examined. It was found that Mfn2 is responsible for tethering mitochondria to SR, which regulates calcium signaling, ATP production, and cell proliferation in VSMCs.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Cell Biology
Karla M. Marquez-Nogueras, Virdjinija Vuchkovska, Elisabeth DiNello, Sara Osorio-Valencia, Ivana Y. Kuo
Summary: Polycystin-2 (PC2) plays a crucial role in myogenesis by regulating intracellular Ca2+ signals and metabolic pathways, affecting the differentiation and mitochondrial remodeling of myoblasts.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Olha M. Koval, Emily K. Nguyen, Dylan J. Mittauer, Karima Ait-Aissa, William C. Chinchankar, Isabella M. Grumbach
Summary: In this study, the role of mitochondrial dysfunction and Ca2+ levels in vascular smooth muscle cell (VSMC) proliferation in type 2 diabetes (T2D) was investigated. The results showed that VSMCs from T2D mice exhibited increased proliferation and altered Ca2+ handling compared to normoglycemic mice. Inhibiting mitochondrial Ca2+ uptake or mitochondrial transition pore (mPTP) had contrasting effects on VSMC proliferation, while inhibiting Ca2+-dependent signaling in the cytosol reduced excessive activation of Erk1/2 and VSMC proliferation. These findings suggest that altered Ca2+ handling and mitochondrial dysfunction contribute to enhanced VSMC proliferation in T2D.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Sean M. Raph, Marc M. Dwenger, Xuemei Hu, Matthew A. Nystoriak
Summary: Smooth muscle voltage-gated K+ (Kv) channels in resistance arteries control vascular tone and contribute to the coupling of blood flow with local metabolic activity. The glycolytic end-product L-lactate and superoxide-derived hydrogen peroxide (H2O2) modulate the activity of Kv1 channels in vascular smooth muscle. L-lactate activates Kv1 channels through the lactate dehydrogenase (LDH)-mediated conversion to pyruvate, while H2O2 activates Kv1 channels in the presence of L-lactate. The Kv beta complex of native vascular Kv1 channels acts as a key effector for multiple redox signals to regulate channel activity and vascular tone.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Gastroenterology & Hepatology
Xiangqin He, Kunzhe Dong, Jian Shen, Guoqing Hu, James D. Mintz, Reem T. Atawia, Juanjuan Zhao, Xiuxu Chen, Robert W. Caldwell, Meixiang Xiang, David W. Stepp, David J. Fulton, Jiliang Zhou
Summary: This study reveals the critical role of Carmn, a specific long noncoding RNA (lncRNA), in regulating the contractility and phenotype of gastrointestinal smooth muscle cells (SMCs). Loss of Carmn function leads to impaired gastrointestinal motility and disease, indicating the essential role of Carmn in maintaining the function of SMCs in the gut.
Review
Physiology
William F. Jackson
Summary: Arterioles in the peripheral microcirculation play a crucial role in regulating blood flow and pressure in tissues and organs. Ca2+-dependent ion channels in vascular smooth muscle cells and endothelial cells are essential for controlling arteriolar tone through both negative and positive feedback mechanisms.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Mikaelle C. Correia, Eder S. A. Santos, Bruno J. Neves, Matheus L. Rocha
Summary: This study evaluated the acute effect of APAP treatment on vascular tone and found that APAP reduced vascular contraction by blocking the influx of calcium ions.
PHARMACOLOGICAL REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Sou Inagaki, Yoshiaki Suzuki, Keisuke Kawasaki, Rubii Kondo, Yuji Imaizumi, Hisao Yamamura
Summary: Mitochondria play a crucial role in cytosolic Ca2+ buffering and energy metabolism. Recent research has shown that Mfn2 regulates Ca2+ signaling by tethering mitochondria and sarco-plasmic reticulum, enhancing mitochondrial function and VSMC proliferation. However, the physiological role of Mfn1 in Ca2+ signaling and mitochondrial function is still unclear.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Geriatrics & Gerontology
Francisco E. Martin-Cano, Cristina Camello-Almaraz, Jesus Gonzalez Macias, Maria J. Pozo, Pedro J. Camello
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2016)
Article
Biochemistry & Molecular Biology
Francisco Eduardo Martin-Cano, Mercedes Caso-Agundez, Cristina Camello-Almaraz, Francisco Juan Santos, Maria Teresa Espin, Juan Antonio Madrid, Adolfo Diez-Perez, Pedro Javier Camello, Maria Jose Pozo
JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY
(2017)
Article
Gastroenterology & Hepatology
Francisco E. Martin-Cano, Pedro J. Camello, Maria J. Pozo
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2014)
Article
Endocrinology & Metabolism
Francisco E. Martin-Cano, Cristina Camello-Almaraz, Dario Acuna-Castroviejo, Maria J. Pozo, Pedro J. Camello
JOURNAL OF PINEAL RESEARCH
(2014)
Article
Oncology
Jose Sanchez-Collado, Jose J. Lopez, Isaac Jardin, Pedro J. Camello, Debora Falcon, Sergio Regodon, Gines M. Salido, Tarik Smani, Juan A. Rosado
Article
Biochemistry & Molecular Biology
Cristina Camello-Almaraz, Francisco E. Martin-Cano, Francisco J. Santos, Ma Teresa Espin, Juan Antonio Madrid, Maria J. Pozo, Pedro J. Camello
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Oncology
Carlos Cantonero, Pedro Javier Camello, Carmen Abate, Francesco Berardi, Gines Maria Salido, Juan Antonio Rosado, Pedro C. Redondo
Article
Pharmacology & Pharmacy
David Hernandez-Moreno, Sara Morales, Cristina Camello-Almaraz, Maria J. Pozo, Pedro J. Camello
Summary: The study found that the naturally occurring oxidant monochloramine affects guinea pig gallbladder contractions in a concentration-dependent manner, involving various biological pathways such as extracellular Ca2+ influx, tyrosine kinase, PKC, MAPK, and NO pathways. NH2Cl not only impairs the gallbladder response to CCK but also to membrane depolarization and nervous plexus stimulation.
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
C. Cantonero, P. J. Camello, G. M. Salido, J. A. Rosado, P. C. Redondo
Summary: TMEM97 is involved in the proliferation of breast cancer cells by regulating calcium influx and promoting cholesterol uptake. It acts by influencing the interaction between SOCE calcium channel and cholesterol in cancer cells.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Article
Cell Biology
Guadalupe Martinez-Chacon, Marta Paredes-Barquero, Sokhna M. S. Yakhine-Diop, Elisabet Uribe-Carretero, Ariadna Bargiela, Maria Sabater-Arcis, Jose Morales-Garcia, Jesus Alarcon-Gil, Eva Alegre-Cortes, Saray Canales-Cortes, Mario Rodriguez-Arribas, Pedro Javier Camello, Jose Manuel Bravo-San Pedro, Ana Perez-Castillo, Ruben Artero, Rosa A. Gonzalez-Polo, Jose M. Fuentes, Mireia Niso-Santano
Summary: QBA is a novel autophagy inducer that triggers autophagy in various neuronal cell lines and mouse and fly models. The beclin-1 (BECN1) and mTOR pathways participate in the regulation of QBA-induced autophagy. Moreover, QBA stimulates SIRT1, which promotes autophagy by the deacetylation of critical ATG proteins. QBA-mediated autophagy promotes neuroprotection in Parkinson's disease in vitro and in a mouse model and extends the lifespan of Drosophila melanogaster.
CELL BIOLOGY AND TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jose Sanchez-Collado, Jose J. Lopez, Isaac Jardin, Alejandro Berna-Erro, Pedro J. Camello, Carlos Cantonero, Tarik Smani, Gines M. Salido, Juan A. Rosado
Summary: The two variants of the Orai1 subunit, Orai1 alpha and Orai1 beta, exhibit different functional characteristics and interactions in mammalian cells. Orai1 alpha is essential for the activation and membrane location of TRPC1, while Orai1 beta plays a role in maintaining Ca2+ oscillations.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cell Biology
Eva Alegre-Cortes, Alberto Gimenez-Bejarano, Elisabet Uribe-Carretero, Marta Paredes-Barquero, Andre R. A. Marques, Mafalda Lopes-da-Silva, Otilia V. Vieira, Saray Canales-Cortes, Pedro J. Camello, Guadalupe Martinez-Chacon, Ana Aiastui, Roberto Fernandez-Torron, Adolfo Lopez de Munain, Patricia Gomez-Suaga, Mireia Niso-Santano, Rosa A. Gonzalez-Polo, Jose M. Fuentes, Sokhna M. S. Yakhine-Diop
Summary: Myotonic dystrophy type 1 (DM1) is an autosomal dominant disease caused by CTG repeat expansion in the 3' untranslated region of the dystrophia myotonica protein kinase gene. Fibroblasts derived from DM1 patients show increased autophagy flux, enlarged endosomes, and lysosomes. The binding of EGF to EGFR and the internalization of EGFR are delayed in DM1 cells, but EGF-activated EGFR enhances the phosphorylation of AKT and ERK1/2.
Article
Cell Biology
Letizia Albarran, Jose J. Lopez, Isaac Jardin, Jose Sanchez-Collado, Alejandro Berna-Erro, Tarik Smani, Pedro J. Camello, Gines M. Salido, Juan A. Rosado
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
(2018)
Meeting Abstract
Clinical Neurology
V. Reyes Garrido, V. E. Fernandez Sanchez, M. V. Castro Sanchez, A. M. Alonso Tones, M. J. Postigo Pozo, M. Villagran Garcia, N. L. Ciano Petersen, G. Pons Pons, M. M. Guerrero Fernandez, A. Leon Martin, T. Munoz Ruiz, P. Urbaneja Romero, P. J. Serrano Castro, O. Fernandez Fernandez
MULTIPLE SCLEROSIS JOURNAL
(2018)