Article
Radiology, Nuclear Medicine & Medical Imaging
Juan Tao, Zhenzhen Yin, Xiangwen Li, Yu Zhang, Kai Zhang, Yanyu Yang, Shaobo Fang, Shaowu Wang
Summary: This study investigated the correlation between IVIM parameters (D, D*, f, and fD* values) and microvessel architecture (MVD, VM, and PCI) in a murine model of rhabdomyosarcoma. The results showed that D*, f, and fD* values were positively correlated with MVD, while D value did not correlate with MVD. D value was negatively correlated with VM, and D* and fD* values were positively correlated with PCI.
EUROPEAN RADIOLOGY
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Kai Zhang, Yue Dai, Yajie Liu, Juan Tao, Ziang Pan, Lizhi Xie, Shaowu Wang
Summary: The correlation between parameters derived from IVIM and DKI and Ki-67 LI in soft tissue sarcoma was explored in this study. MK was found to be the optimal imaging biomarker for assessing the Ki-67 expression of STS.
EUROPEAN RADIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Rex E. Jeffries, Shawn M. Gomez, Jeffrey M. Macdonald, Michael P. Gamcsik
Summary: Nuclear magnetic resonance (NMR) spectroscopy was used to monitor the metabolism of glutathione in alginate-encapsulated JM-1 hepatoma cells. The results showed that the C-13-labeled glutathione signal provided a high-intensity spectrum, allowing for the monitoring of the detailed kinetics of glutathione metabolism. Additionally, the effects of the thiol alkylating agent on glutathione metabolism were observed, and the regeneration of intracellular glutathione was studied through the restoration of the C-13-glutathione signal and the wash-out of the conjugate.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Chemistry, Multidisciplinary
Florence Franconi, Laurent Lemaire, Jean-Christophe Gimel, Samuel Bonnet, Patrick Saulnier
Summary: NMR-based diffusion methods are valuable tools for nanomedicine characterization and understanding interactions with the biological environment. They provide insights into diffusion phenomena and measurement of self-diffusion and mutual diffusion coefficients. NMR diffusometry spectroscopic and imaging methods have broad applications in nanomedicine, aiding in elucidating important issues.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Physics, Multidisciplinary
Ainitze Biteri-Uribarren, Pol Alsina-Bolivar, Carlos Munuera-Javaloy, Ricardo Puebla, Jorge Casanova
Summary: This study tackles the detection of individual molecules by combining a hybrid sensor-a nitrogen vacancy center (NV) and a dangling bond on the diamond surface-with a multi-tone dynamical decoupling sequence. Via numerical simulations, the authors prove that the sequence minimizes the impact of decoherence, which allows using the dangling-bond as a signal amplifier.
COMMUNICATIONS PHYSICS
(2023)
Article
Chemistry, Physical
Yannik Hinz, Joachim Beerwerth, Roland Boehmer
Summary: This research investigates the motion of chloride ions in glyceline using various nuclear magnetic resonance techniques and finds highly disordered coordination of anions. At higher temperatures, chloride ions are coupled with the structural units in glyceline, while at lower temperatures, the motion of anions becomes faster and the overall ionic conductivity displays a super-Arrhenius behavior, indicating dominant charge transport by cations rather than anions.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Biochemical Research Methods
Olivia A. Fraser, Kevin E. W. Namitz, Scott A. Showalter
Summary: Post-translational modifications (PTMs) are reversible chemical modifications that can affect protein structure and function. Methylation and acetylation are two well-known PTMs with important biological roles, but their specific effects on transcription factor structure and function are not well understood. This study presents a nondestructive and label-free method using nuclear magnetic resonance (NMR) to detect methylation and acetylation events on histone H3 tail. The authors introduce isotopic enrichment and 13C labeling schemes that enable efficient NMR experiments for detecting low-abundance analytes.
Article
Chemistry, Physical
Yannik Hinz, Joachim Beerwerth, Roland Boehmer
Summary: This study examines the motions of Cl- within a glycerol: choline chloride solution using various nuclear magnetic resonance techniques. The results show that the chlorine ions are coupled to the glycerol and choline moieties at high temperatures, but become faster than the matrix molecules at lower temperatures. Furthermore, the charge transport is dominated by choline cations rather than Cl anions throughout the temperature range.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Quantum Science & Technology
Soham Pal, Priya Batra, Tanjung Krisnanda, Tomasz Paterek, T. S. Mahesh
Summary: Quantum entanglement is a unique form of correlation between quantum particles that cannot be increased through local operations and classical communication. The presence of entanglement gain between detectors interacting through a classical mediator suggests non-classical characteristics of the mediator. Research indicates the significance of the initial state in determining the entanglement gain between detectors.
Article
Chemistry, Analytical
Yao Wei, Hua Jiang, Pengchi Deng
Summary: A method was established to quantitatively determine Cys and GSH by studying their interaction with β-CD AgNPs using chemical shift analysis. The method exhibited good linearity and low detection limits, and was successfully applied for quantifying Cys and GSH in human serum and urine.
MICROCHEMICAL JOURNAL
(2021)
Article
Medicine, General & Internal
Vy Tran, Anne Hong, Tom Sutherland, Kim Taubman, Su-Faye Lee, Daniel Lenaghan, Kapil Sethi, Niall M. Corcoran, Nathan Lawrentschuk, H. Woo, Lisa Tarlinton, Damien Bolton, Tim Spelman, Lauren Thomas, Russell Booth, Justin Hegarty, Elisa Perry, Lih-Ming Wong
Summary: This study aims to evaluate the diagnostic accuracy of 18F DCFPyL PSMA PET scans combined with multiparametric MRI in detecting primary prostate cancer prior to biopsy. A total of 240 subjects will be recruited for the study, and diagnostic accuracy will be assessed after software coregistration of mpMRI and PSMA-PET/CT images. The results of this study will have significant implications for the management of prostate cancer patients.
Article
Physics, Multidisciplinary
Wentian Zheng, Ke Bian, Xiakun Chen, Yang Shen, Shichen Zhang, Rainer Stohr, Andrej Denisenko, Jorg Wrachtrup, Sen Yang, Ying Jiang
Summary: An efficient method was developed to engineer the electrostatic environment of near-surface nitrogen vacancy center qubits, increasing their coherence and sensitivity.
Article
Chemistry, Analytical
Lihua Xu, Biling Huang, Zhiying Hou, Shaohua Huang, Yufen Zhao
Summary: This study demonstrates the simultaneous analysis of a complex mixture using solvent effects by F-19 NMR spectroscopy, which enables the identification and quantification of multiple amino acids. This method has important implications for human health and disease diagnosis.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Quentin Stern, Samuel Francois Cousin, Frederic Mentink-Vigier, Arthur Cesar Pinon, Stuart James Elliott, Olivier Cala, Sami Jannin
Summary: Dynamic nuclear polarization is a widely used tool for enhancing the sensitivity of nuclear magnetic resonance spectroscopy and imaging, but its practical applicability is limited by the spin diffusion barrier, which hinders efficient polarization transfer between highly polarized nuclei and bulk nuclei.
Article
Multidisciplinary Sciences
Hsueh-Ying Chen, C. Blake Wilson, Robert Tycko
Summary: The spatial resolution of MRI is limited by signal detection sensitivity, but can be improved by performing measurements at low temperature and using dynamic nuclear polarization (DNP) technique. This allows for higher resolution and shorter data acquisition times, offering a promising direction for high-resolution MRI studies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Katelyn E. Connelly, Tyler M. Weaver, Aktan Alpsoy, Brian X. Gu, Catherine A. Musselman, Emily C. Dykhuizen
NUCLEIC ACIDS RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Catherine A. Musselman, Tatiana G. Kutateladze
ACS CHEMICAL BIOLOGY
(2019)
Article
Chemistry, Multidisciplinary
Christopher S. Eubanks, Bo Zhao, Neeraj N. Patwardhan, Rhese D. Thompson, Qi Zhang, Amanda E. Hargrove
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Article
Multidisciplinary Sciences
Tyler M. Weaver, Jiachen Liu, Katelyn E. Connelly, Chris Coble, Katayoun Varzavand, Emily C. Dykhuizen, Catherine A. Musselman
SCIENTIFIC REPORTS
(2019)
Article
Biochemical Research Methods
Rhese D. Thompson, Jared T. Baisden, Qi Zhang
Article
Biochemical Research Methods
Bo Zhao, Jared T. Baisden, Qi Zhang
JOURNAL OF MAGNETIC RESONANCE
(2020)
Article
Biochemistry & Molecular Biology
Jared T. Baisden, Joshua A. Boyer, Bo Zhao, Scott M. Hammond, Qi Zhang
Summary: Research has shown that the precursor of oncogenic microRNA-21 can form a pH-dependent ensemble that reshuffles its structure spontaneously, leading to enhanced efficiency in Dicer processing. This suggests that the efficiency of microRNA biogenesis may be encoded in the intrinsic dynamic ensemble of primary and precursor microRNAs, allowing regulation in response to environmental and cellular stimuli.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Joshua A. Boyer, Cathy J. Spangler, Joshua D. Strauss, Andrew P. Cesmat, Pengda Liu, Robert K. McGinty, Qi Zhang
Article
Multidisciplinary Sciences
Yanqiong Zhang, Zhe Ma, Ying Wang, Joshua Boyer, Guoxin Ni, Liang Cheng, Siyuan Su, Zhigang Zhang, Zhichuan Zhu, Jiayi Qian, Lishan Su, Qi Zhang, Blossom Damania, Pengda Liu
Review
Biochemistry & Molecular Biology
Mohamed Ghoneim, Harrison A. Fuchs, Catherine A. Musselman
Summary: The core histone tails are crucial for chromatin structure and signaling, yet their conformation in a chromatin context has been difficult to determine. Recent studies suggest that histone tails may form a high-affinity fuzzy complex with DNA in nucleosomes and nucleosome arrays, influencing chromatin structure and signaling transitions.
TRENDS IN BIOCHEMICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Emma A. Morrison, Lokesh Baweja, Michael G. Poirier, Jeff Wereszczynski, Catherine A. Musselman
Summary: Hexasomes and tetrasomes are key intermediates in nucleosome assembly and disassembly, whose formation is regulated by histone chaperones and other factors. Changes in nucleosome composition, including the H3 tail conformation, can potentially impact chromatin signaling and landscape, suggesting a regulatory role in gene expression. The dynamic interactions between histone tails and DNA within these structures may play a significant role in chromatin function and gene regulation.
NUCLEIC ACIDS RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Catherine A. Musselman, Tatiana G. Kutateladze
Summary: The spatial and temporal modulation of chromatin structure is crucial for regulating DNA templated processes, and the nucleosome, as the basic subunit of chromatin, plays a key role in this regulation. Recent studies using NMR spectroscopy have revealed the atomic level conformation and dynamics of the nucleosome and provided a better understanding of the importance of these dynamics in key regulatory events.
ACS CHEMICAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kaeli A. Welsh, Derek L. Bolhuis, Anneroos E. Nederstigt, Joshua Boyer, Brenda R. S. Temple, Thomas Bonacci, Li Gu, Alban Ordureau, J. Wade Harper, Joshua P. Steimel, Qi Zhang, Michael J. Emanuele, Joseph S. Harrison, Nicholas G. Brown
Summary: Polyubiquitination mediated by E2 and E3 enzymes is essential for cell cycle control, epigenetic regulation, and development. The helix-turn-helix (HTH) motif of the UBC has been shown to regulate the intrinsic polyubiquitination activity in different E2s, with distinct functions observed in UBE2S and UBE2R2. The repurposing of the E2(HTH) motif in these proteins suggests a new mechanism for modulating ubiquitin transfer and highlights the potential for additional regulation in E2-E3-mediated polyubiquitination.
Article
Biochemistry & Molecular Biology
Saumya M. De Silva, Alisha Dhiman, Surbhi Sood, Kilsia F. Mercedes, William J. Simmons, Morkos A. Henen, Beat Vogeli, Emily C. Dykhuizen, Catherine A. Musselman
Summary: PBRM1 is a subunit of the PBAF chromatin remodeling complex and is frequently mutated in clear cell renal cell carcinoma patients. It functions as a chromatin binding subunit and contains six tandem bromodomains which can bind nucleosomes acetylated at histone H3 lysine 14 (H3K14ac). In addition, the second and fourth bromodomains of PBRM1 also have the ability to bind double stranded RNA elements.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Edgar M. Faison, Amrita Nallathambi, Qi Zhang
Summary: RNA molecules undergo conformational transitions in response to cellular and environmental stimuli, which can be regulated by site-specific protonation. A new method, pH-differential mutational profiling (PD-MaP) with dimethyl sulfate probing, was developed for high-throughput detection of protonation-coupled conformational ensembles in RNA. The application of this method on microRNA-21 precursor (pre-miR-21) revealed known A(+)-G-coupled conformational ensemble and identified a secondary protonation event involving an A(+)-C mismatch. Validation of these protonation-coupled ensembles was achieved using NMR relaxation dispersion spectroscopy. Furthermore, widespread protonation-coupled conformational ensembles were found in a library of well-annotated human primary microRNAs, suggesting their broad functions in biology.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)