Journal
JOURNAL OF MAGNETIC RESONANCE
Volume 310, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jmr.2019.106642
Keywords
RNA dynamics; Chemical exchange; Riboswitch; Ligand binding
Funding
- NIH [R01 GM114432]
- NSF [MCB1652676]
- University of North Carolina at Chapel Hill
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Characterizing low-populated and short-lived excited conformational states has become increasingly important for understanding mechanisms of RNA function. Interconversion between RNA ground and excited conformational states often involves base pairing rearrangements that lead to changes in the hydrogen-bond network. Here, we present two N-15 chemical exchange saturation transfer (CEST) NMR experiments that utilize protonated and non-protonated nitrogens, which are key hydrogen-bond donors and acceptors, for characterizing excited conformational states in RNA. We demonstrated these approaches on the B. Cereus fluoride riboswitch, where N-15 CEST profiles complement C-13 CEST profiles in depicting a potential pathway for ligand-dependent allosteric regulation of the excited conformational state of the fluoride riboswitch. (C) 2019 Elsevier Inc. All rights reserved.
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