Article
Polymer Science
Migle Babelyte, Laura Peciulyte, Vesta Navikaite-Snipaitiene, Joana Bendoraitiene, Volodymyr Samaryk, Ramune Rutkaite
Summary: Thermoresponsive chitosan-graft-poly(N-isopropylacrylamide) copolymers with different compositions were synthesized. The chemical structure of the copolymers was confirmed and their lower critical solution temperature (LCST) behavior was assessed. Copolymers with a certain content of poly(N-isopropylacrylamide) side chains demonstrated LCST behavior and could potentially be used in drug delivery systems or tissue engineering.
Article
Polymer Science
Chia-Hsin Ko, Cristiane Henschel, Geethu P. Meledam, Martin A. Schroer, Peter Mueller-Buschbaum, Andre Laschewsky, Christine M. Papadakis
Summary: The temperature-dependent phase behavior and self-assembly behavior of a thermoresponsive amphiphilic diblock copolymer in aqueous solution were studied, revealing a lower critical solution temperature (LCST) behavior at 31.1 degrees Celsius and the formation of different structures of micelles.
Article
Polymer Science
Parvaneh Gheysoori, Azin Paydayesh, Maryam Jafari, Hamed Peidayesh
Summary: This study focuses on the development of thermoresponsive nanocomposite hydrogels for minimally invasive drug delivery. Gelatin-based hydrogels with poly (N-isopropylacrylamide) (PNIPAM) were prepared using response surface methodology. The PNIPAM nanoparticles showed successful polymerization and a reversible sol-gel transition close to body temperature. These nanocomposite hydrogels demonstrated antibacterial activity and good potential for targeted drug delivery.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Polymer Science
Sihao Xu, Francisco J. Trujillo, Jiangtao Xu, Cyrille Boyer, Nathaniel Corrigan
Summary: In this study, a series of PNIPAm homopolymers with narrow MWDs were prepared via PET-RAFT polymerization, and their thermal transition temperatures were analyzed. It was found that higher order statistical parameters of the MWDs also influence the thermoresponsive transition of the binary blends of PNIPAm samples.
MACROMOLECULAR RAPID COMMUNICATIONS
(2021)
Article
Chemistry, Physical
Sung Ho Park, Sang Joon Lee
Summary: This study presents a new facile and fast approach for the selective recovery of lithium ions from seawater using an aluminum crosslinked poly(N-isopropylacrylamide) (PNIPAAm)/alginate (Alg) adsorbent. The experimental results demonstrate that the thermoresponsive IPN gel exhibits high adsorption efficiency for lithium ions and can extract them with low thermal energy.
GREEN ENERGY & ENVIRONMENT
(2022)
Article
Chemistry, Physical
Wei Liu, Yuwei Zhu, Tong Zhang, Hui Zhu, Chuanxin He, To Ngai
Summary: This study investigated the rheological responses of soft PNIPAM microgel dispersions during volume phase transitions, revealing significant differences between near-wall and bulk systems, with the former exhibiting segmented overlap behavior and different physical states in various φeff regions. The local structures near the wall show more heterogeneity in glass and colloidal gel states compared to the liquid state.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Nanoscience & Nanotechnology
Yu-Chen Huang, Qian-Pu Cheng, U-Ser Jeng, Shan-hui Hsu
Summary: The development of hydrogel-based actuators with programmable deformation has attracted much attention in both fundamental and applied research. In this study, a temperature-responsive and biodegradable gelatin methacryloyl (GelMA)-poly(N-isopropylacrylamide) hydrogel (GN hydrogel) was explored as the active layer of a bilayer actuator. The optimized bilayer hydrogel actuator exhibited large, reproducible, and anisotropic bending, and has potential applications in biomimetic devices, artificial muscles, and soft robotics for biomedical purposes.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
David Joram Mendoza, Meri Ayurini, Christine Browne, Vikram Singh Raghuwanshi, George P. Simon, Joel F. Hooper, Gil Garnier
Summary: A family of thermoresponsive poly(N-isopropyla-crylamide)-grafted cellulose nanofibers was synthesized via a silver-promoted decarboxylative polymerization approach. The method allows for control over functionalization and grafting amount, and the resulting grafted nanofibers exhibit remarkable thermoresponsive properties and can be used as thermosensitive biomaterials. This study validates a novel approach for the synthesis of new thermoresponsive and transparent hydrogels.
Review
Polymer Science
Atsushi Narumi, Shin-ichiro Sato, Xiande Shen, Toyoji Kakuchi
Summary: This paper describes the progress in precision polymerizations of specific N-alkylacrylamides and N,N-dialkylacrylamides to produce thermoresponsive polymers in aqueous media. Early research focused on synthesizing polyacrylamides with well-defined structures. Later studies explored the effects of polymer structure on thermoresponsive properties. The paper also discusses various polymerization methodologies and the synthesis and thermoresponsive properties of polymers with specific structures.
Article
Chemistry, Physical
Haiyang Cui, Lingling Zhang, Dominik Soeder, Xiaomei Tang, Mehdi D. Davari, Ulrich Schwaneberg
Summary: MetRich plays a crucial role in the rapid immobilization of CueO on electrodes, showing high affinity to carbon nanotubes through the synergistic effect of pi-pi stacking and hydrophobic interactions. Fusion of MetRich with CotA enables CotA to rapidly and selectively adsorb on electrode surfaces.
Article
Materials Science, Multidisciplinary
Evgenii A. Ksendzov, Pavel A. Nikishau, Irina M. Zurina, Viktoria S. Presniakova, Peter Timashev, Yuri A. Rochev, Svetlana Kotova, Sergei Kostjuk
Summary: A series of well-defined macromonomers and copolymers were synthesized via ROP and RAFT-mediated copolymerization, respectively. The cloud point temperature and transition temperature of the graft copolymers showed dependence on the copolymer composition. Films prepared from these copolymers displayed better cell adhesion compared to neat PNIPAM.
ACS APPLIED POLYMER MATERIALS
(2022)
Article
Pharmacology & Pharmacy
Aysu Yurdasiper, Asli Sahiner, Evren Homan Gokce
Summary: This study aimed to develop triclosan nanogels of poly(N-isopropylacrylamide) with thermoresponsive character for the treatment of acne vulgaris. It was observed that the swelling capacity of the nanogel formulations increased with the addition of triclosan and ethanol. All nanogel formulations exhibited viscoelastic behavior at 25℃, with higher elastic modulus G' at that temperature compared to 37℃. The encapsulation efficiency was found to be 47.4% and 63.7%. The nanogel formulation showed superior antibacterial activity against C. acnes compared to the commercial product. Overall, the thermoresponsive nanogel formulation of poly(N-isopropylacrylamide) demonstrated suitability for dermal applications and could potentially be an alternative to conventional formulations for the treatment of acne vulgaris.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Polymer Science
Todsapol Kajornprai, Putita Katesripongsa, Sang Yong Nam, Zuratul Ain Abdul Hamid, Yupaporn Ruksakulpiwat, Nitinat Suppakarn, Tatiya Trongsatitkul
Summary: This study demonstrated the potential applications of thermoresponsive poly(N-isopropylacrylamide) (PNIPAm) grafted nylon membranes through different grafting yields and architecture. The membranes exhibited thermoresponsive gating performance below and above the lower critical solution temperature of PNIPAm. The study also showed excellent oil/water separation and gas barrier properties of the membranes, indicating their suitability for various applications.
Article
Polymer Science
Bo Pang, Rui Liu, Guang Han, Wei Wang, Wangqing Zhang
Summary: The eight-arm star poly(N-isopropylacrylamide) based on POSS was synthesized via RAFT polymerization using both Z-RAFT and R-RAFT strategies. The Z-type POSS-(PNIPAM)(8) obtained from the Z-RAFT strategy showed well-defined structure, while polymers with broad molecular weight distribution were obtained using the R-RAFT strategy. The star POSS-(PNIPAM)(8) exhibited a narrow temperature window for phase transition, forming small-sized aggregates above the PNIPAM arms' phase transition temperature and decreasing oil/water interfacial tension. Emulsification-demulsification was reversible by adjusting temperature.
Article
Polymer Science
Xiaoyan Xu, Kentaro Shibata, Makoto Ouchi
Summary: In this study, a alternating copolymer with thermoresponsive behavior was synthesized via ATRP-based cyclopolymerization. The unique structural features of the alternating sequence affected the dehydration behavior of the copolymer.
Article
Biochemistry & Molecular Biology
Michalina Zaborowska, Damian Dziubak, Dorota Matyszewska, Slawomir Sek, Renata Bilewicz
Summary: The study involved four model lipid films with different compositions, showing that an equimolar content of the three components led to the formation of a stable and well-organized assembly with well-developed raft microdomains. This provides a convenient model membrane for further research on interactions with drugs or pollutants, or the incorporation of membrane proteins.
Review
Biochemistry & Molecular Biology
Agnieszka Walewska, Milena Krajewska, Aleksandra Stefanowska, Aleksandra Buta, Renata Bilewicz, Pawel Krysinski, Piotr Bednarczyk, Piotr Koprowski, Adam Szewczyk
Summary: This paper critically reviews the techniques used in studying the function of mitochondrial potassium channels. The evaluation focuses on the advantages and limitations of these techniques. The functional analysis of mitochondrial potassium channels presents additional challenges compared to plasma membrane channels. The paper critically evaluates three types of techniques and presents new possible approaches for studying mitochondrial potassium channels. It aims to assist researchers in selecting reliable methods and in evaluating the reported results.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Przemyslaw Miszta, Ewa Nazaruk, Dorota Nieciecka, Mariusz Mozajew, Pawel Krysinski, Renata Bilewicz, Slawomir Filipek
Summary: Lipidic cubic phases were used as a convenient environment to study the transport properties of the membrane antiporter EcCLC, showing more efficient chloride ion transport at acidic pH and decreased film resistance at lower pH. The inhibitor DIDS decreased transport efficiency upon hydrolysis to DADS at both pH values. Molecular dynamics simulations in lipidic cubic phases allowed for studying collective movements of chloride ions to elucidate the mechanism of ion transport by the EcCLC antiporter. These findings suggest that the protein-modified lipidic cubic phase film is a simple system for screening potential inhibitors of integral membrane proteins, and lipidic cubic phases may be important for developing new electrochemical sensors for membrane proteins and enzyme electrodes.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)
Article
Electrochemistry
Agnieszka Wieckowska, Elzbieta Jablonowska, Maciej Dzwonek, Marcin Jaskolowski, Renata Bilewicz
Summary: This study investigated the assembly of Au nanoclusters with lipids and the formation of hybrid monolayers on electrodes. The Langmuir-Blodgett technique was used to control the composition and organization of the mixed monolayer at the air-water interface. The electrochemical properties of the modified electrode were studied and the Au nanoclusters were found to act as electron transfer mediators.
Article
Biochemistry & Molecular Biology
Aleksandra Buta, Ewa Nazaruk, Damian Dziubak, Adam Szewczyk, Renata Bilewicz
Summary: The lipid cubic phase (LCP) is a nanomaterial composed of water channels surrounded by lipid bilayers, making it favorable for embedding membrane proteins. In this study, we investigated the effect of embedding the ionophoric peptide gramicidin A (gA) on the structure and properties of the LCP film. Our results demonstrated that the LCP can serve as a suitable 3D biomimetic film for the study of ion channels and other transporting membrane proteins.
BIOELECTROCHEMISTRY
(2022)
Article
Medicine, Research & Experimental
Adrianna Cytryniak, Kinga Zelechowska-Matysiak, Ewa Nazaruk, Renata Bilewicz, Rafai Walczak, Emilia Majka, Adam Mames, Frank Bruchertseifer, Alfred Morgenstern, Aleksander Bilewicz, Agnieszka Majkowska-Pilip
Summary: In this study, tailored lipid liquid-crystalline carriers (cubosomes) were proposed as a strategy to enhance the effectiveness of anticancer drugs. These carriers incorporated both an anticancer drug (doxorubicin) and a short-lived alpha-emitter (bismuth-213). The experiments showed that the presence of a specific ligand affected the activity of the drug, and the release profile of the drug was influenced by electrostatic interactions. The sequential exposure of cancer cells to radiolabeled cubosomes and drug-doped cubosomes resulted in a significant decrease in cell viability.
MOLECULAR PHARMACEUTICS
(2022)
Article
Materials Science, Biomaterials
Aleksandra Bartkowiak, Ewa Nazaruk, Ewa Gajda, Marlena Godlewska, Damian Gawel, Elzbieta Jablonowska, Renata Bilewicz
Summary: Understanding the mechanism of how simvastatin enhances the penetration of the chemotherapeutic agent doxorubicin into cell membranes, this study found that coadministration of simvastatin and doxorubicin facilitates the penetration of neutral lipid monolayers, leading to improved drug delivery efficiency. Furthermore, the combination of doxorubicin and simvastatin showed enhanced anticancer effects in cancer cells.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Michalina Zaborowska, Dorota Matyszewska, Renata Bilewicz
Summary: HMG-CoA reductase, a membrane protein responsible for cholesterol synthesis, was successfully incorporated into lipid rafts mimicking cell membranes. The enzyme activity was confirmed through dynamic light scattering and fluorescence microscopy. Thin lipid raft films were investigated using the Langmuir technique, showing preserved activity over time.
Article
Materials Science, Multidisciplinary
Olga Swiech, Maciej Majdecki, Renata Bilewicz
Summary: This study presents a multicomponent polymer material for electrochemical sensing, consisting of polyethylene glycol, nanoparticles modified with a lipoic acid derivative of cyclodextrin, and a biocompatible cross linker. The material enhances the sensor's selectivity and detection limit through improved analyte binding and conductivity. It was successfully used for the detection of ferrocene derivative and ibuprofen at low concentrations.
ACS APPLIED POLYMER MATERIALS
(2023)
Article
Chemistry, Analytical
Agnieszka Wieckowska, Elzbieta Jablonowska, Marcin Jaskolowski, Renata Bilewicz
Summary: Metal nanostructures are commonly used in thin films to enhance the conductivity of electrodes. In this study, we show that even at very low surface concentrations, gold clusters act as remote electrodes, facilitating electron transfer to solution species when embedded in a lipid film. The presence of gold clusters enables efficient electron transfer through the lipid barrier, as demonstrated by the electrode processes of hexaammineruthenium(III). Reversibility of the electrochemical probe is restored with the addition of as low as 0.01 mol% of Au clusters dispersed in the outer leaflet of the lipid bilayer.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Michalina Zaborowska, Aleksandra Bartkowiak, Ewa Nazaruk, Dorota Matyszewska, Renata Bilewicz
Summary: The Langmuir technique was used to compare the layers obtained by spreading lipid liquid-crystalline nanoparticles with the monolayers obtained by mixing the same components in chloroform at the air-water interface. The isotherms obtained for the mixed components system and the cubosome-derived layer showed similarity, indicating the disintegration of cubosomes into a single monolayer upon contact with the air-water interface. AFM imaging confirmed the presence of intact cubosomes on the surface of mica when performed in water. This study provides insights into the behavior of lipid nanoparticles at interfaces.
Review
Chemistry, Analytical
Martina Zatloukalova, Lukasz Poltorak, Renata Bilewicz, Jan Vacek
Summary: Some biologically active substances are unstable and poorly soluble in aqueous media, which limits their bioavailability. Incorporating these compounds into lipid-based liquid crystalline phases or nanoparticles can enhance their stability, transport properties, bioavailability, and applicability. This overview aims to explain the self-assembly principle of lipidic amphiphilic molecules in water and present the current biosensing and biomedical applications of lipidic bicontinuous cubic and hexagonal phases, with a focus on electrochemical protocols.
Article
Chemistry, Physical
Michalina Zaborowska, Marcin Broniatowski, Philippe Fontaine, Renata Bilewicz, Dorota Matyszewska
Summary: In this study, the effect of statins on model lipid rafts and their components was investigated using a combined GIXD/PM-IRRAS/BAM approach for the first time. It was found that the hydrophobicity of the drugs and the organization of the lipid layer determined the drug-lipid interaction.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Article
Nanoscience & Nanotechnology
Kornelia Bobrowska, Kamila Sadowska, Krzysztof Stolarczyk, Marta Przesniak-Welenc, Piotr Golec, Renata Bilewicz
Summary: In this study, hybrid nanoflowers based on bovine serum albumin (BSA) and hydroxyapatite (HA) were successfully synthesized and characterized. The nanoflowers were able to embed ciprofloxacin and release it in a controlled manner. Additionally, the nanoflowers exhibited antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
A. Buta, E. Nazaruk, B. Kulawiak, P. Koprowski, A. Szewczyk, R. Bilewicz