Article
Chemistry, Analytical
Jun-Hao Wan, Chen Qian, Gang Wu, Xian-Wei Liu
Summary: Label-free imaging of nanoscale targets with intrinsic properties is crucial for unveiling the underlying mechanisms of chemistry, physics, and life science. Plasmonic imaging techniques provide real-time imaging and insights into nanoscale detection and nanocatalysis. In this study, a high-resolution plasmonic imaging method is presented, demonstrating its capability to image various nanomaterials and accurately track the interfacial dynamics of nanoparticles. With the simplicity and capacity for label-free and real-time imaging, this approach serves as a promising platform for characterizing nanomaterials at the single-particle level.
ANALYTICAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Hyun-Kyu Choi, Hyun Gyu Kim, Min Ju Shon, Tae-Young Yoon
Summary: Single-molecule magnetic tweezers provide magnetic force and torque to single target molecules, allowing the study of dynamic changes in biomolecular structures and interactions. Using smaller magnetic beads and shorter tethers improves dynamic response times and measurement precision. High-resolution single-molecule magnetic tweezers can track nanometer changes in target molecules on a millisecond time scale.
ANNUAL REVIEW OF BIOCHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Natalie Fardian-Melamed, Liat Katrivas, Dvir Rotem, Alexander Kotlyar, Danny Porath
Summary: In recent years, the novel guanine octuplex DNA has attracted much interest as a stable and rigid nanostructure. Electronic structure studies have shown that its lower energy gap results in higher transverse conductivity compared to guanine quadruplex DNA.
Article
Chemistry, Physical
Ji-Hoon Lee, Joyce Han-Ching Chiu, Nicholas J. J. Ginga, Tasdiq Ahmed, M. D. Thouless, Yifan Liu, Shuichi Takayama
Summary: This study developed a three-layer tunable nanochannel system that enables non-electrophoretic linearization and immobilization of native chromatin. Through careful selection of self-blinking fluorescent dyes and nanochannel design, they achieved super-resolution imaging of the linearized chromatin using direct stochastic optical reconstruction microscopy (dSTORM). This work opens up a new avenue for collecting long-range and high-resolution epigenetic information as well as genetic information.
NANOSCALE HORIZONS
(2023)
Article
Biochemical Research Methods
John S. H. Danial, Jeff Y. L. Lam, Yunzhao Wu, Matthew Woolley, Eleni Dimou, Matthew R. Cheetham, Derya Emin, David Klenerman
Summary: This article presents an economic and easy-to-assemble single-molecule localization microscopy setup called NanoPro for high-throughput super-resolution imaging. It provides step-by-step instructions and illustrations to aid researchers in constructing and utilizing NanoPro, as well as assessing its performance.
Article
Chemistry, Physical
Yang Du, Ying Lai, Ji-Yan Liu, Jiajie Diao
Summary: This article presents a new ultrasensitive method for detecting 5hmC modification in DNA, which requires a minimal amount of DNA sample and can provide results within a short period of time.
Article
Chemistry, Physical
Johannes Zirkelbach, Masoud Mirzaei, Irena Deperasinska, Boleslaw Kozankiewicz, Burak Gurlek, Alexey Shkarin, Tobias Utikal, Stephan Goetzinger, Vahid Sandoghdar
Summary: We investigate the vibrational levels of dye molecules in solid matrices using high-resolution spectroscopic measurements. By employing narrow-band continuous-wave lasers and fluorescence excitation and stimulated emission depletion techniques, we are able to assess individual vibrational linewidths in the electronic ground state. Our findings reveal exceptionally narrow vibronic levels and provide insights into the vibrational behavior of single molecules at the nanoscale. This work has implications for applications in quantum technology.
JOURNAL OF CHEMICAL PHYSICS
(2022)
Article
Chemistry, Multidisciplinary
Mike Filius, Sung Hyun Kim, Ivo Severins, Chirlmin Joo
Summary: The research team introduced a new method that allows for determining multiple distances between FRET pairs in a single object by resolving the FRET efficiency of multiple fluorophore pairs through transient binding of short DNA strands. This FRET X technology is expected to be a tool for high-resolution analysis of biomolecules and nanostructures.
Article
Chemistry, Multidisciplinary
Jiao-Na Han, Caijun Zhong, Mingmin Ge, Shi Kuang, Zhou Nie
Summary: The NHCouI dual-emission chromophore is a G4-responsive probe with a red emission signal that specifically responds to parallel G4s and a green emission signal that acts as an internal reference signal. It can self-calibrate the signal and avoid viscosity disturbances during G4 imaging in living cells.
Article
Multidisciplinary Sciences
Gang Wu, Chen Qian, Wen-Li Lv, Xiaona Zhao, Xian-Wei Liu
Summary: Direct visualization of surface chemical dynamics in solution is challenging but essential for understanding nanocatalysis and electrochemistry. We present an azimuth-modulated plasmonic imaging technique that achieves high spatial resolution and enables comprehensive analyses of surface chemical dynamics. This method eliminates interference from reflected light, resulting in a 67-fold increase in the spatial resolution of plasmonic imaging. The technique is demonstrated using electrochemical redox reactions over single silver nanowires, showcasing its potential for studying interfacial chemical reactions.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Hamish C. L. Yau, Adam K. Malekpour, Nazarmohammad G. Momin, Ana L. Morales-Garcia, William G. T. Willats, Neil J. Lant, Catherine Y. Jones
Summary: Research has found that microbial extracellular DNA plays a key role in the cleaning process of textiles, and using DNase enzymes can effectively remove these eDNA, improving washing results. The use of immunostaining and confocal microscopy has demonstrated the cleaning benefits of DNase enzymes on fabrics by reducing microbial DNA.
SCIENTIFIC REPORTS
(2021)
Article
Materials Science, Multidisciplinary
W. Dednam, S. Tewari, E. B. Lombardi, J. J. Palacios, J. M. van Ruitenbeek, C. Sabater
Summary: This article studies the dynamic bonding of gold atoms on surfaces under low coordination conditions using low-temperature scanning tunneling microscopy. By conducting experiments with an atomically sharp gold tip and utilizing classical molecular dynamics simulations and density-functional theory calculations, the differences in bonding behavior between different positions are elucidated, as well as providing information on the crystalline classification of the STM tips based on their performance.
Article
Biochemistry & Molecular Biology
Uwe Schwartz, Maria Llamazares Prada, Stephanie T. Pohl, Mandy Richter, Raluca Tamas, Michael Schuler, Corinna Keller, Vedrana Mijosek, Thomas Muley, Marc A. Schneider, Karsten Quast, Joschka Hey, Claus P. Heussel, Arne Warth, Hauke Winter, Oezdemirhan Sercin, Harry Karmouty-Quintana, Soma S. K. Jyothula, Manish K. Patel, Felix Herth, Ina Koch, Giuseppe Petrosino, Alexandru Titimeaua, Balca R. Mardin, Dieter Weichenhan, Tomasz P. Jurkowski, Charles D. Imbusch, Benedikt Brors, Vladimir Benes, Birgit Jung, David Wyatt, Heiko F. Stahl, Christoph Plass, Renata Z. Jurkowska
Summary: Patients with chronic obstructive pulmonary disease (COPD) are still waiting for curative treatments. We hypothesized that COPD is associated with altered epigenetic signaling in lung cells. We generated genome-wide DNA methylation maps of primary human lung fibroblasts (HLFs) across COPD stages and found early epigenetic changes in COPD, predominantly in regulatory regions. Dysregulation of genes involved in proliferation, DNA repair, and extracellular matrix organization were observed. Furthermore, we identified candidate regulators and demonstrated the potential for novel therapeutic avenues for COPD patients.
Article
Optics
Mingyue Song, Zhaohua Yang, Ping Li, Zidong Zhao, Ying Liu, Yuanjin Yu, Ling-An Wu
Summary: We propose a measurement system based on single-pixel imaging with compressive sensing that can achieve high spectral and spatial resolution simultaneously while allowing data compression. Our method breaks the mutually restrictive relationship between spectral and spatial resolution in traditional imaging. Experimental results show that our system obtains 301 spectral channels in the 420-780 nm band with a spectral resolution of 1.2 nm and a spatial resolution of 1.11 mrad. By utilizing compressive sensing, we achieve a sampling rate of 12.5% for a 64 x 64 pixel image, significantly reducing the measurement time and enabling simultaneous high spectral and spatial resolution.
Article
Engineering, Electrical & Electronic
Haotian Shi, Huiyu Qi, Guangyue Shen, Zhaohui Li, Guang Wu
Summary: Achieving precise underwater 3D imaging is crucial for marine research and development. However, the strong scattering of water causes laser beams to spread out, resulting in low-resolution imaging. In this paper, we demonstrate a single-photon LiDAR with Bessel beam illumination, which greatly improves imaging resolution in long-distance underwater sensing.
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2022)
Article
Biochemical Research Methods
Natalia Borovok, Elimelech Nesher, Yishai Levin, Michal Reichenstein, Albert Pinhasov, Izhak Michaelevski
MOLECULAR & CELLULAR PROTEOMICS
(2016)
Article
Nanoscience & Nanotechnology
Gennady Eidelshtein, Alexander Kotlyar, Mohtadin Hashemi, Leonid Gurevich
Article
Chemistry, Multidisciplinary
Gennady Eidelshtein, Natalie Fardian-Melamed, Vitaly Gutkin, Dmitry Basmanov, Dmitry Klinov, Dvir Rotem, Yael Levi-Kalisman, Danny Porath, Alexander Kotlyar
ADVANCED MATERIALS
(2016)
Article
Neurosciences
Y. Pen, N. Borovok, M. Reichenstein, A. Sheinin, I. Michaelevski
Article
Chemistry, Multidisciplinary
Gennady Eidelshtein, Moran Fattal, Gavriel Avishai, Benjamin Kempinski, Clelia Giannini, Alexander Kotlyar
Article
Biochemical Research Methods
Natalia Borovok, Elimelech Nesher, Michal Reichenstein, Tatiana Tikhonova, Yishai Levin, Albert Pinhasov, Izhak Michaelevski
PROTEOMICS CLINICAL APPLICATIONS
(2017)
Review
Genetics & Heredity
Gabor Zsurka, Viktoriya Peeva, Alexander Kotlyar, Wolfram S. Kunz
Article
Chemistry, Multidisciplinary
Gideon I. Livshits, Jamal Ghabboun, Natalia Borovok, Alexander B. Kotlyar, Danny Porath
ADVANCED MATERIALS
(2014)
Article
Nanoscience & Nanotechnology
Gideon I. Livshits, Avigail Stern, Dvir Rotem, Natalia Borovok, Gennady Eidelshtein, Agostino Migliore, Erika Penzo, Shalom J. Wind, Rosa Di Felice, Spiros S. Skourtis, Juan Carlos Cuevas, Leonid Gurevich, Alexander B. Kotlyar, Danny Porath
NATURE NANOTECHNOLOGY
(2014)
Article
Biochemical Research Methods
Liat Katrivas, Benjamin Kempinski, Kfir Kuchuk, Uri Sivan, Alexander Kotlyar
BIOCONJUGATE CHEMISTRY
(2019)
Article
Nanoscience & Nanotechnology
Galina Proshkina, Sergey Deyev, Anastasiya Ryabova, Francesco Tayanti, Maria Cristina Menziani, Roy Cohen, Liat Katrivas, Alexander Kotlyar
ACS APPLIED MATERIALS & INTERFACES
(2019)
Article
Genetics & Heredity
Natalia Borovok, Celeste Weiss, Rajech Sharkia, Michal Reichenstein, Bernd Wissinger, Abdussalam Azem, Muhammad Mahajnah
FRONTIERS IN GENETICS
(2020)
Article
Oncology
Galina Proshkina, Elena Shramova, Anastasiya Ryabova, Liat Katrivas, Clelia Giannini, Daniele Malpicci, Yael Levi-Kalisman, Sergey Deyev, Alexander Kotlyar
Summary: This study demonstrates a methodology to enhance the solubility of peptide nucleic acid (PNA) and delivers it specifically to cancer cells using designed ankyrin repeat protein (DARPin)-functionalized small multilamellar vesicles (SMVs). The positively charged PNA molecules bind electrostatically to the negatively charged phospholipid membranes, encapsulating the PNA in the vesicles. The DARPin-functionalized SMVs can enter HER2-overexpressing cells and cause cell death by interfering with normal cell functioning.
Article
Biochemical Research Methods
Sergey Deyev, Galina Proshkina, Anastasiya Ryabova, Francesco Tavanti, Maria Cristina Menziani, Gennady Eidelshtein, Gavriel Avishai, Alexander Kotlyar
BIOCONJUGATE CHEMISTRY
(2017)
Article
Pharmacology & Pharmacy
Yaron Dekel, Yossy Machluf, Tal Gefen, Gennady Eidelshtein, Alexander Kotlyar, Yaron Bram, Ehud Shahar, Farah Reslane, Elina Aizenshtein, Jacob Pitcovski
