Review
Genetics & Heredity
Guangzhao Guan, Richard D. D. Cannon, Dawn E. E. Coates, Li Mei
Summary: The mechanical properties of cells play an important role in tissue homeostasis and various cellular processes. These properties are determined by the cytoskeleton, which is a complex and dynamic network of microfilaments, intermediate filaments, and microtubules. The Rho-kinase/ROCK signaling pathway regulates the architecture of this cytoskeletal network, influencing cell behavior.
Article
Cell Biology
Shiri Avivi Kela, Kriti Sethi, Pei Yi Tan, Danesha Suresh, Hui Ting Ong, Perla G. Castaneda, Mustafi R. Amin, Tal Laviv, Erin J. Cram, Jan Faix, Ronen Zaidel-Bar
Summary: This study identified a novel mechanotransduction pathway in the C. elegans spermatheca, which involves tension-dependent localization of a RhoGEF and a positive feedback loop for RHO-1-MyosinII activation. It provides insights into how epithelial tubes can sense and adjust their contractility in response to internal pressure.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Mengwei Li, Limei Zhang, Xinyan Liu, Guoqiang Wang, Jian Lu, Jifeng Guo, Hongjie Wang, Jinpeng Xu, Yi Zhang, Na Li, You Zhou
Summary: This study found that low-intensity exercise training can improve the heart function and structure of spontaneously hypertensive rats by inhibiting the RhoA/ROCK signaling pathway, providing further evidence for this protective effect.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Liding Zhao, Ya Li, Tian Xu, Qingbo Lv, Xukun Bi, Xianglan Liu, Guosheng Fu, Yunzeng Zou, Junbo Ge, Zhaoyang Chen, Wenbin Zhang
Summary: This study found that diabetes mellitus plays a significant role in aggravating chronic inflammation and promoting atherosclerosis, in conjunction with hyperlipidemia. The mechanism of action may involve the induction of immune maturation of dendritic cells, likely through the RAGE-TLR4-PKC β(1) signaling pathway.
MOLECULAR MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Takuya Nakashima, Riku Tanibe, Hiroto Yoshida, Masahiro Ehara, Miwa Kuzuhara, Tsuyoshi Kawai
Summary: Pathway-dependent chirality control in silver nanoclusters is achieved by using enantiomeric ligand, leading to the formation of chiral silver nanoclusters with intrinsic chirality. Guest binding to the nanoclusters' shell can reverse the stability between right- and left-handed nanoclusters, resulting in unprecedented chirality inversion.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Food Science & Technology
Ping Tang, Xinzhou Yang, Huijian Chen, Ting Zhang, Hui Tang, Kejian Pang
Summary: This study demonstrates the antidiabetic activity of ethyl acetate extract of Morus nigra L. twigs (MNT-EA) and its potential mechanisms through the activation of AMPK/PKC/GLUT4 signaling pathway, which enhances glucose uptake and improves diabetic symptoms.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Pharmacology & Pharmacy
Fatma Tugce Dalkir, Fatma Aydinoglu, Nuran Ogulener
Summary: This study investigated the role of RhoA/Rho-kinase (ROCK) and PKC in the inhibitory effect of L-cysteine/hydrogen sulfide (H2S) pathway on carbachol-induced contraction in mouse bladder smooth muscle. L-cysteine and H2S reduced the contractions, and ROCK and PKC inhibitors also decreased the contractions, suggesting an interaction between L-cysteine/H2S and the RhoA/ROCK pathway. The inhibitory effect of L-cysteine on ROCK-1, pMYPT1, and pMLC20 levels was reversed by PAG, indicating that CSE-generated H2S mediates the inhibition.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)
Article
Physics, Multidisciplinary
Tomasz Skora, Mathijs Janssen, Andreas Carlson, Svyatoslav Kondrat
Summary: Macromolecular crowding can have significant impacts on various biophysical processes, including diffusion, gene expression, cell growth, and senescence. However, the effects of crowding on reactions, especially multivalent binding, are not fully understood. In this study, we used scaled particle theory and molecular simulation to investigate the binding of monovalent to divalent biomolecules. Our findings show that crowding can greatly enhance or reduce cooperativity, depending on the sizes of the involved molecular complexes. Furthermore, crowding can enable binding that would not occur otherwise.
PHYSICAL REVIEW LETTERS
(2023)
Article
Cell Biology
Irena Audzeyenka, Dorota Rogacka, Patrycja Rachubik, Marlena Typiak, Michal Rychlowski, Stefan Angielski, Agnieszka Piwkowska
Summary: The study demonstrates that the PKGI alpha-dependent Rac1 signaling pathway enhances insulin-mediated glucose uptake in podocytes, while downregulation of PKGI alpha or Rac1 expression abolishes this effect. Silencing of Rac1 prevents actin remodeling and GLUT4 translocation to the cell membrane.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Review
Endocrinology & Metabolism
Taylor Landry, Daniel Shookster, Hu Huang
Summary: ROCK1 plays important roles in metabolism, with potential as a therapeutic target in diabetes and obesity. Recent advances in tissue-specific approaches have helped to uncover its critical metabolic functions.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Cell Biology
Ignacio Garcia, Sara Orellana-Munoz, Lucia Ramos-Alonso, Aram N. Andersen, Christine Zimmermann, Jens Eriksson, Stig Ove Boe, Petra Kaferle, Manolis Papamichos-Chronakis, Pierre Chymkowitch, Jorrit M. Enserink
Summary: Cells use mechanisms to detect signals and respond, with Kel1 serving as a noise suppressor in the yeast pheromone pathway, preventing cell death by inhibiting membrane protein recruitment to prevent spontaneous activation of the pathway.
Article
Cardiac & Cardiovascular Systems
Rosalinda Madonna, Stefania Moscato, Maria Concetta Cufaro, Damiana Pieragostino, Letizia Mattii, Piero Del Boccio, Sandra Ghelardoni, Riccardo Zucchi, Raffaele De Caterina
Summary: This study found that empagliflozin can inhibit excessive autophagy in the heart of diabetic patients by suppressing the AMPK/GSK3β signaling pathway, thus protecting the heart from damage.
CARDIOVASCULAR RESEARCH
(2023)
Article
Physiology
Chuan Jiang, Ning Dong, Jianli Feng, Maolin Hao
Summary: miR-190 plays a protective role against brain I/R damage by modulating Rho/Rho-kinase signaling.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Tetsu Kimura, Yuta Horikoshi, Chika Kuriyagawa, Yukitoshi Niiyama
Summary: Ischemic strokes (IS) and spinal cord injuries (SCI) are major causes of disability. The up-regulation of the RhoA/ROCK pathway contributes to neuronal apoptosis, neuroinflammation, blood-brain barrier dysfunction, astrogliosis, and axon growth inhibition in IS and SCI. Inhibiting the Rho/ROCK pathway with miRNAs alleviates apoptosis, neuroinflammation, oxidative stress, and axon growth inhibition in IS and SCI. Further studies are required to explore the significance of ncRNAs in IS and SCI and to establish new strategies for preventing and treating these devastating diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Physiology
Taiki Mori, Yuji Hotta, Daigaku Nakamura, Ryo Yahagi, Tomoya Kataoka, Kazunori Kimura
Summary: This study investigated the mechanisms of stress-induced erectile dysfunction using a rat model, and found that stress can lead to ED through the RhoA/Rho kinase pathway. Additionally, stress also decreases the smooth muscle/collagen ratio of corpus cavernosum, which was not improved by treatment with fasudil.
PHYSIOLOGICAL REPORTS
(2021)