Transposon sequencing: methods and expanding applications

A new platform for ultra-high density Staphylococcus aureus transposon libraries

(2015) Marina Santiago et al.   BMC GENOMICS

A Genome-Wide Screen Reveals that the Vibrio cholerae Phosphoenolpyruvate Phosphotransferase System Modulates Virulence Gene Expression

(2015) Qiyao Wang et al.   INFECTION AND IMMUNITY

Genome-Wide Transposon Mutagenesis Indicates that Mycobacterium marinum Customizes Its Virulence Mechanisms for Survival and Replication in Different Hosts

(2015) Eveline M. Weerdenburg et al.   INFECTION AND IMMUNITY

Transposon Mutagenesis Paired with Deep Sequencing of Caulobacter crescentus under Uranium Stress Reveals Genes Essential for Detoxification and Stress Tolerance

(2015) Mimi C. Yung et al.   JOURNAL OF BACTERIOLOGY

Essential genome ofPseudomonas aeruginosain cystic fibrosis sputum

(2015) Keith H. Turner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

General and condition-specific essential functions ofPseudomonas aeruginosa

(2015) Samuel A. Lee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genetic determinants of in vivo fitness and diet responsiveness in multiple human gut Bacteroides

(2015) M. Wu et al.   SCIENCE

Antimicrobial peptide resistance mediates resilience of prominent gut commensals during inflammation

(2015) T. W. Cullen et al.   SCIENCE

Fitness cost of antibiotic susceptibility during bacterial infection

(2015) Damien Roux et al.   Science Translational Medicine

High-Throughput Analysis of Gene Essentiality and Sporulation in Clostridium difficile

(2015) Marcin Dembek et al.   mBio

Genetic Basis of Persister Tolerance to Aminoglycosides in Escherichia coli

(2015) Yue Shan et al.   mBio

Genome-Wide Identification of Klebsiella pneumoniae Fitness Genes during Lung Infection

(2015) Michael A. Bachman et al.   mBio

Rapid Quantification of Mutant Fitness in Diverse Bacteria by Sequencing Randomly Bar-Coded Transposons

(2015) Kelly M. Wetmore et al.   mBio

Essential Genes in the Core Genome of the Human Pathogen Streptococcus pyogenes

(2015) Yoann Le Breton et al.   Scientific Reports

Essential Role of the ESX-5 Secretion System in Outer Membrane Permeability of Pathogenic Mycobacteria

(2015) Louis S. Ates et al.   PLoS Genetics

Molecular Characterization of a Multidrug Resistance IncF Plasmid from the Globally Disseminated Escherichia coli ST131 Clone

(2015) Minh Duy Phan et al.   PLoS One

Genomic Analyses of Pneumococci from Children with Sickle Cell Disease Expose Host-Specific Bacterial Adaptations and Deficits in Current Interventions

(2014) Robert Carter et al.   Cell Host & Microbe

High-Throughput Sequencing of Campylobacter jejuni Insertion Mutant Libraries Reveals mapA as a Fitness Factor for Chicken Colonization

(2014) J. G. Johnson et al.   JOURNAL OF BACTERIOLOGY

Escherichia coli Genes and Pathways Involved in Surviving Extreme Exposure to Ionizing Radiation

(2014) R. T. Byrne et al.   JOURNAL OF BACTERIOLOGY

Improving the Efficiency of Transposon Mutagenesis in Salmonella Enteritidis by Overcoming Host-Restriction Barriers

(2014) Turki M. Dawoud et al.   MOLECULAR BIOTECHNOLOGY

Identification ofin vivoregulators of theVibrio cholerae xdsgene using a high-throughput genetic selection

(2014) EmilyKate McDonough et al.   MOLECULAR MICROBIOLOGY

High-resolution genetic analysis of the requirements for horizontal transmission of the ESBL plasmid from Escherichia coli O104:H4

(2014) Yoshiharu Yamaichi et al.   NUCLEIC ACIDS RESEARCH

Genome-Wide High-Throughput Screening to Investigate Essential Genes Involved in Methicillin-Resistant Staphylococcus aureus Sequence Type 398 Survival

(2014) Mette T. Christiansen et al.   PLoS One

Global discovery of colonization determinants in the squid symbiontVibrio fischeri

(2014) John F. Brooks et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Compound-gene interaction mapping reveals distinct roles for Staphylococcus aureus teichoic acids

(2014) J. P. Santa Maria et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genome-Wide Identification of Acinetobacter baumannii Genes Necessary for Persistence in the Lung

(2014) N. Wang et al.   mBio

Genes Contributing to Staphylococcus aureus Fitness in Abscess- and Infection-Related Ecologies

(2014) M. D. Valentino et al.   mBio

Release of Nonstop Ribosomes Is Essential

(2014) Heather A. Feaga et al.   mBio

Shielding of a Lipooligosaccharide IgM Epitope Allows Evasion of Neutrophil-Mediated Killing of an Invasive Strain of Nontypeable Haemophilus influenzae

(2014) J. D. Langereis et al.   mBio

Genome-Wide Mutant Fitness Profiling Identifies Nutritional Requirements for Optimal Growth of Yersinia pestis in Deep Tissue

(2014) S. G. Palace et al.   mBio

Genome-Wide Saturation Mutagenesis of Burkholderia pseudomallei K96243 Predicts Essential Genes and Novel Targets for Antimicrobial Development

(2014) M. G. Moule et al.   mBio

Illuminating the future of DNA sequencing

(2014) Mick Watson   GENOME BIOLOGY

Homopolymer tail-mediated ligation PCR: a streamlined and highly efficient method for DNA cloning and library construction

(2013) David W. Lazinski et al.   BIOTECHNIQUES

Tryptophan Biosynthesis Protects Mycobacteria from CD4 T-Cell-Mediated Killing

(2013) Yanjia J. Zhang et al.   CELL

Tn-Seq Analysis of Vibrio cholerae Intestinal Colonization Reveals a Role for T6SS-Mediated Antibacterial Activity in the Host

(2013) Yang Fu et al.   Cell Host & Microbe

Streptococcus pneumoniae Folate Biosynthesis Responds to Environmental CO2 Levels

(2013) P. Burghout et al.   JOURNAL OF BACTERIOLOGY

A Genomewide Mutagenesis Screen Identifies Multiple Genes Contributing to Vi Capsular Expression in Salmonella enterica Serovar Typhi

(2013) D. Pickard et al.   JOURNAL OF BACTERIOLOGY

Nontypeable Haemophilus influenzae Carbonic Anhydrase Is Important for Environmental and Intracellular Survival

(2013) J. D. Langereis et al.   JOURNAL OF BACTERIOLOGY

Deciphering the genetic basis ofMoraxella catarrhaliscomplement resistance: a critical role for the disulphide bond formation system

(2013) Stefan P. W. de Vries et al.   MOLECULAR MICROBIOLOGY

Transposon insertion sequencing: a new tool for systems-level analysis of microorganisms

(2013) Tim van Opijnen et al.   NATURE REVIEWS MICROBIOLOGY

A comparison of dense transposon insertion libraries in the Salmonella serovars Typhi and Typhimurium

(2013) Lars Barquist et al.   NUCLEIC ACIDS RESEARCH

Characterization of the Molecular Interplay between Moraxella catarrhalis and Human Respiratory Tract Epithelial Cells

(2013) Stefan P. W. de Vries et al.   PLoS One

Genome-scale analysis of gene function in the hydrogenotrophic methanogenic archaeon Methanococcus maripaludis

(2013) F. Sarmiento et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Identification of T6SS-dependent effector and immunity proteins by Tn-seq in Vibrio cholerae

(2013) T. G. Dong et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genome-wide fitness profiling reveals adaptations required by Haemophilus in coinfection with influenza A virus in the murine lung

(2013) S. M. Wong et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Approaches to querying bacterial genomes with transposon-insertion sequencing

(2013) Lars Barquist et al.   RNA Biology

Comprehensive Assignment of Roles for Salmonella Typhimurium Genes in Intestinal Colonization of Food-Producing Animals

(2013) Roy R. Chaudhuri et al.   PLoS Genetics

The Serum Resistome of a Globally Disseminated Multidrug Resistant Uropathogenic Escherichia coli Clone

(2013) Minh-Duy Phan et al.   PLoS Genetics

Genome-Wide Detection of Fitness Genes in Uropathogenic Escherichia coli during Systemic Infection

(2013) Sargurunathan Subashchandrabose et al.   PLoS Pathogens

A Comprehensive Analysis of In Vitro and In Vivo Genetic Fitness of Pseudomonas aeruginosa Using High-Throughput Sequencing of Transposon Libraries

(2013) David Skurnik et al.   PLoS Pathogens

Gene Fitness Landscapes of Vibrio cholerae at Important Stages of Its Life Cycle

(2013) Heather D. Kamp et al.   PLoS Pathogens

Genome Scanning for Conditionally Essential Genes in Salmonella enterica Serotype Typhimurium

(2012) Anita Khatiwara et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Identification of essential genes of the periodontal pathogen Porphyromonas gingivalis

(2012) Brian A Klein et al.   BMC GENOMICS

Anomalies of the anaerobic tricarboxylic acid cycle inShewanella oneidensisrevealed by Tn-seq

(2012) Evan D. Brutinel et al.   MOLECULAR MICROBIOLOGY

Control of Virulence by Small RNAs in Streptococcus pneumoniae

(2012) Beth Mann et al.   PLoS Pathogens

Global Assessment of Genomic Regions Required for Growth in Mycobacterium tuberculosis

(2012) Yanjia J. Zhang et al.   PLoS Pathogens

pBAM1: an all-synthetic genetic tool for analysis and construction of complex bacterial phenotypes

(2011) Esteban Martínez-García et al.   BMC MICROBIOLOGY

Retrospective Application of Transposon-Directed Insertion Site Sequencing to a Library of Signature-Tagged Mini-Tn5Km2 Mutants of Escherichia coli O157:H7 Screened in Cattle

(2011) S. E. Eckert et al.   JOURNAL OF BACTERIOLOGY

The essential genome of a bacterium

(2011) B. Christen et al.   Molecular Systems Biology

Systematic Comparison of Three Methods for Fragmentation of Long-Range PCR Products for Next Generation Sequencing

(2011) Ellen Knierim et al.   PLoS One

Advances in bacterial transcriptome and transposon insertion-site profiling using second-generation sequencing

(2011) Melanie Febrer et al.   TRENDS IN BIOTECHNOLOGY

Are pathogenic bacteria just looking for food? Metabolism and microbial pathogenesis

(2011) Laurence Rohmer et al.   TRENDS IN MICROBIOLOGY

Genome-Scale Identification of Resistance Functions in Pseudomonas aeruginosa Using Tn-seq

(2011) L. A. Gallagher et al.   mBio

High-Resolution Phenotypic Profiling Defines Genes Essential for Mycobacterial Growth and Cholesterol Catabolism

(2011) Jennifer E. Griffin et al.   PLoS Pathogens

Identifying Genetic Determinants Needed to Establish a Human Gut Symbiont in Its Habitat

(2009) Andrew L. Goodman et al.   Cell Host & Microbe

Experimental approaches for the discovery and characterization of regulatory small RNA

(2009) Cynthia Mira Sharma et al.   CURRENT OPINION IN MICROBIOLOGY

Simultaneous assay of every Salmonella Typhi gene using one million transposon mutants

(2009) G. C. Langridge et al.   GENOME RESEARCH

Global discovery of adaptive mutations

(2009) Hani Goodarzi et al.   NATURE METHODS

Tn-seq: high-throughput parallel sequencing for fitness and genetic interaction studies in microorganisms

(2009) Tim van Opijnen et al.   NATURE METHODS

Tracking insertion mutants within libraries by deep sequencing and a genome-wide screen for Haemophilus genes required in the lung

(2009) J. D. Gawronski et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Systematic Mapping of Genetic Interaction Networks

(2008) Scott J. Dixon et al.   Annual Review of Genetics

Identification of Campylobacter jejuni Genes Contributing to Acid Adaptation by Transcriptional Profiling and Genome-Wide Mutagenesis

(2008) A. N. Reid et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

The Chemical Genomic Portrait of Yeast: Uncovering a Phenotype for All Genes

(2008) M. E. Hillenmeyer et al.   SCIENCE