Article
Biochemistry & Molecular Biology
Andrej Kovacik, Petra Pullmannova, Lukas Opalka, Michaela Silarova, Jaroslav Maixner, Katerina Vavrova
Summary: The study shows that different types of ceramides play distinct roles in the structural organization and function of the skin lipid barrier, with their effects on lipid models varying depending on the sphingoid base chain and configuration at the alpha-carbon.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Physical
Dunja Vidovic, Natasa Milosevic, Nebojsa Pavlovic, Nemanja Todorovic, Jelena Canji Panic, Strahinja Kovacervic, Milica Karadzic Banjac, Sanja Podunavac-Kuzmanovic, Nebojsa Banjac, Nemanja Trisovic, Bojan Bozic, Mladena Lalic-Popovic
Summary: The percutaneous permeation of newly synthesized succinimide derivatives was evaluated using the PAMPA method with silicone oil and isopropyl myristate. In silico predictions and experimental results showed that most compounds had good skin permeability, but two compounds with carboxylic groups had limited permeation at pH 7.4. The study found a significant association between in silico predicted logKp values and experimental permeability, indicating the importance of lipophilicity in passive permeation through hydrophobic barriers.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Article
Medicine, Research & Experimental
Silvia Franze, Francesco Rama, Paolo Rocco, Michela Debernardi, Valeria Bincoletto, Silvia Arpicco, Francesco Cilurzo
Summary: This study demonstrates the potential of controlling drug delivery in the skin by adjusting the amount of HA on the vesicle surface. Different phosphatidylethanolamine-HA conjugates impact the deformability of liposomes and the transdermal permeation of drugs, with higher HA extent favoring drug retention in the skin.
MOLECULAR PHARMACEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Jiaxin Shen, Yanping Wu, Teng Wei, Yangzheng He, Xiaoru Liu, Zeyuan Deng, Jing Li
Summary: Phospholipids are crucial for the growth and neurodevelopment of infants. Human milk phospholipid analogs (HMPAs) show better digestion and absorption characteristics compared to soybean phospholipids (SPLs), which are commonly used in infant formulas.
Article
Pharmacology & Pharmacy
Aneta Vovesna, Alexander Zhigunov, Martin Balouch, Jarmila Zbytovska
Summary: The study indicated that stable liposomal systems containing ceramides and other skin barrier lipids are effective in repairing a disrupted skin barrier, and non-homogenized liposomes are more effective than their homogenized counterparts.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Chemistry, Multidisciplinary
Masato Miyauchi, Shinkichi Ishikawa, Takeshi Kurachi, Kazutami Sakamoto, Hideki Sakai
Summary: This study investigated the effects of inhaled aerosols on oral tissues, finding that aerosol exposure had no effect on the viability, morphology, and structure of the tissues. Majority of the aerosol transferred to the airway tissues, and the distribution from inner tissue to outer tissue was selective. Nicotine absorbed in the oral cavity was partially transferred to the inner tissue and partially to the gastrointestinal tract through swallowing, while water-soluble compounds with hydroxyl groups were dissolved in saliva and transferred to the gastrointestinal tract through swallowing.
Review
Biotechnology & Applied Microbiology
Terrence T. Roh, Ying Chen, Sara Rudolph, Michelle Gee, David L. Kaplan
Summary: There are inherent differences between human and animal systems, thus the development of in vitro models is crucial for understanding the intestinal innate immune system.
TRENDS IN BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Michael S. Harris, Rachel F. Dolan, James R. Bryce, Jonas G. Ewusi, Gabriel A. Cook
Summary: Membrane glycoproteins are difficult to study but are linked to human diseases. Using membrane mimetics, researchers have successfully solubilized and performed glycosylation of a full-length membrane glycoprotein in nanodiscs, providing a biologically relevant sample to study the effects of the modification.
Article
Biochemistry & Molecular Biology
Jee-hyun Hwang, Haengdueng Jeong, Nahyun Lee, Sumin Hur, Nakyum Lee, Jeong Jun Han, Hye Won Jang, Wang Keun Choi, Ki Taek Nam, Kyung-Min Lim
Summary: The study found that the ex vivo live full-thickness porcine skin model is an excellent alternative to animal experiments for research on the safety and efficacy of cosmetic ingredients. Lactobionic acid showed the best improvement in skin barrier function, and the model could measure TEWL, confirming the significant skin barrier-protective effects of PHAs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Balint Sinko, Vivien Bardos, Daniel Vesztergombi, Szabina Kadar, Petra Malcsiner, Anne Moustie, Chantal Jouy, Krisztina Takacs-Novak, Sebastien Gregoire
Summary: The study used Skin-PAMPA to measure the permeability of PER in different solvents and compared it with pig skin permeation data, showing a good correlation (R-2 = 0.844) between the two. This supports the use of Skin-PAMPA for screening suitable solvents for non-polar compounds in the early stages of formulation development.
Article
Pharmacology & Pharmacy
Barbora Amelie Curikova-Kindlova, Aneta Vovesna, Anna Novackova, Jarmila Zbytovska
Summary: This study utilized pig ear skin models to investigate three different methods of skin barrier disruption, demonstrating the effectiveness of lipid mixtures in repairing skin barrier. The study evaluated the restoration potential of the developed models using permeation studies and FTIR spectroscopy.
Article
Chemistry, Medicinal
Edward Price, J. Cory Kalvass, David DeGoey, Balakrishna Hosmane, Stella Doktor, Kelly Desino
Summary: This study evaluated different models for predicting human intestinal absorption data, providing a resource for DMPK scientists and medicinal/computational chemists to enhance their understanding of the utility and applications of permeability and absorption models for academia and industry.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Meliha Ekinci, Emine Esin Caliskan, Burak Cakar, Derya Ilem-Ozdemir, Yigit Uyanikgil, Emel Oyku Cetin Uyanikgil
Summary: The aim of this research was to develop and evaluate the cancer cell incorporation capacity of technetium-99m ([99mTc]Tc)-radiolabeled niosomes. Niosome formulations were developed and characterized, and then radiolabeled with [99mTc]Tc. The cell incorporation of radiolabeled niosomes in cancer cells was assessed and showed higher percentages compared to the control.
Article
Biochemistry & Molecular Biology
Charlotte M. Beddoes, Denise E. Rensen, Gert S. Gooris, Marc Malfois, Joke A. Bouwstra
Summary: The study found that the effects of FFA chain lengths on lamellar phases and lateral packing vary, with a threshold for water permeability at FFA C16.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Engineering, Biomedical
Minjun Ahn, Won-Woo Cho, Wonbin Park, Jae-Seong Lee, Min-Ju Choi, Qiqi Gao, Ge Gao, Dong-Woo Cho, Byoung Soo Kim
Summary: Human skin, as the outermost organ of the body, shows visible signs of physiological health. The need for alternative in vitro platforms that mimic the structural and functional characteristics of natural skin has increased due to ethical concerns and genetic differences in conventional animal studies. Different reproducible biofabrication strategies are required to reproduce the pathological features of diseased human skin compared to those used for healthy-skin models.
BIOMATERIALS RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Julia F. Alopaeus, Marie Hellfritzsch, Tobias Gutowski, Regina Scherliess, Andreia Almeida, Bruno Sarmento, Natasa Skalko-Basnet, Ingunn Tho
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2020)
Article
Chemistry, Medicinal
May Wenche Joraholmen, Abhilasha Bhargava, Kjersti Julin, Mona Johannessen, Natasa Skalko-Basnet
Review
Pharmacology & Pharmacy
Sybil Obuobi, Natasa Skalko-Basnet
Article
Chemistry, Medicinal
Margherita Falavigna, Mette Klitgaard, Ragna Berthelsen, Anette Muellertz, Goril Eide Flaten
Summary: A new in vitro lipolysis-permeation model was developed to predict the in vivo absorption of fenofibrate in SNEDDSs, combining intestinal lipolysis and mucus-PVPA permeation models. The model showed potential for predicting in vivo absorption data by assessing drug permeation after in vitro lipolysis, with a good correlation between in vitro and in vivo AUCs.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Margherita Falavigna, Sunniva Brurok, Mette Klitgaard, Goril Eide Flaten
Summary: The study combined high-throughput in vitro intestinal lipolysis model with mucus-PVPA in vitro permeation model to investigate the solubilization capacity and drug permeation of SNEDDSs for fenofibrate. Results showed a significant impact of in vitro lipolysis on drug solubilization and permeation profiles. The correlation between in vitro permeation data and in vivo plasma concentration in rats was excellent, indicating the predictive ability of the developed combined in vitro model for in vivo drug absorption.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Pharmacology & Pharmacy
May Wenche Joraholmen, Mona Johannessen, Kirsten Gravningen, Mirja Puolakkainen, Ganesh Acharya, Purusotam Basnet, Natasa Skalko-Basnet
Review
Pharmacology & Pharmacy
Zahari Vinarov, Bertil Abrahamsson, Per Artursson, Hannah Batchelor, Philippe Berben, Andreas Bernkop-Schnurch, James Butler, Jens Ceulemans, Nigel Davies, Didier Dupont, Goril Eide Flaten, Nikoletta Fotaki, Brendan T. Griffin, Vincent Jannin, Janneke Keemink, Filippos Kesisoglou, Mirko Koziolek, Martin Kuentz, Alan Mackie, Antonio J. Melendez-Martinez, Mark McAllister, Anette Mullertz, Caitriona M. O'Driscoll, Neil Parrott, Jadwiga Paszkowska, Petr Pavek, Christopher J. H. Porter, Christos Reppas, Cordula Stillhart, Kiyohiko Sugano, Elena Toader, Katerina Valentova, Maria Vertzoni, Saskia N. De Wildt, Clive G. Wilson, Patrick Augustijns
Summary: The review article discusses the challenges and gaps in understanding oral drug absorption processes, focusing on specific patient populations, regional differences in the gastrointestinal tract, advanced formulations, and food-drug interactions. Controversies and gaps in current knowledge are identified to pave the way for future research in the field of oral drug absorption.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Article
Chemistry, Medicinal
Lisa Myrseth Hemmingsen, Kjersti Julin, Luqman Ahsan, Purusotam Basnet, Mona Johannessen, Natasa Skalko-Basnet
Summary: In an era of antimicrobial resistance, novel treatment options are urgently needed to improve the prevention and eradication of infectious skin biofilm-producing pathogens, while enhancing wound healing. Tailoring formulations for acute skin injuries is crucial for proper skin retention and release of antimicrobials.
Article
Pharmacology & Pharmacy
Jennifer Cauzzo, Nikhil Jayakumar, Balpreet Singh Ahluwalia, Azeem Ahmad, Natasa Skalko-Basnet
Summary: The study identifies limitations of traditional methods, introduces QPM as a promising complementary technique, and successfully locates and tracks liposomes.
Article
Chemistry, Applied
Lisa Myrseth Hemmingsen, Barbara Giordani, Ann Kristin Pettersen, Beatrice Vitali, Purusotam Basnet, Natasa Skalko-Basnet
Summary: The incorporation of chlorhexidine (CHX) liposomes into chitosan hydrogel significantly enhances its antimicrobial activity, leading to effective eradication of bacteria and biofilms, with potential therapeutic applications for skin infections.
CARBOHYDRATE POLYMERS
(2021)
Article
Pharmacology & Pharmacy
Marek Soltys, David Zuza, Tereza Boleslavska, Sarah Machac Akhlasova, Martin Balouch, Pavel Kovacik, Josef Beranek, Natasa Skalko-Basnet, Goril Eide Flaten, Frantisek Stepanek
Summary: This study systematically compared the sorption of poorly aqueous soluble active pharmaceutical ingredients to different mesoporous silica carriers, and identified potential pathways for enhancing bioavailability through the rational selection of parameters.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Pharmacology & Pharmacy
Laura Victoria Schulte-Werning, Anjanah Murugaiah, Bhupender Singh, Mona Johannessen, Rolf Einar Engstad, Natasa Skalko-Basnet, Ann Mari Holsaeter
Summary: The development of multifunctional nanofibrous wound dressings containing chloramphenicol, beta-glucan, and chitosan shows promising anti-inflammatory and antimicrobial properties for the treatment of infected and exuding wounds.
Article
Pharmacology & Pharmacy
Ivo Laidmae, Andres Meos, Irja Alainezhad Kjaervik, Sveinung G. Ingebrigtsen, Natasa Skalko-Basnet, Kalle Kirsimae, Tavo Romann, Urmas Joost, Vambola Kisand, Karin Kogermann
Summary: The study compared the preparation of drug-loaded liposomes using two methods and found that liposomes prepared by electrospinning were smaller and more homogeneous but contained less drug. Additionally, the electrospinning method was faster for in-situ liposome preparation.
Article
Biochemistry & Molecular Biology
Philip Rainsford, B. Ravdna Sarre, Margherita Falavigna, Bjorn Olav Brandsdal, Goril Eide Flaten, Martin Jakubec, Johan Isaksson
Summary: Water/Ion NMR Detected - Phospholipid Vesicle Permeability Assay (WIND-PVPA) is a novel method for assessing lipid barrier integrity in vitro. It quantifies the permeability of water and ions to evaluate the disruptive influence of membrane-active molecules, and compares the results with molecular dynamics simulations.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2022)
Article
Materials Science, Biomaterials
Lisa Myrseth Hemmingsen, Barbara Giordani, Marianne H. Paulsen, Zeljka Vanic, Goril Eide Flaten, Beatrice Vitali, Purusotam Basnet, Annette Bayer, Morten B. Strom, Natasa Skalko-Basnet
Summary: The eradication of bacteria in biofilms is a major challenge in treating chronic wounds. Antimicrobial peptides and their mimics are potential options, but their clinical use is limited. A study explored the use of a synthetic mimic of an antimicrobial peptide incorporated into liposomes to improve its anti-biofilm effect and anti-inflammatory activity. The results showed that the liposomes effectively eradicated biofilms and reduced inflammation, making them a promising therapeutic option for bacteria-infected chronic wounds.
BIOMATERIALS ADVANCES
(2023)