A high-efficiency recombineering system with PCR-based ssDNA in Bacillus subtilis mediated by the native phage recombinase GP35

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Full-length single-stranded PCR product mediated chromosomal integration in intact Bacillus subtilis

(2012) Sai Wen et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Bacillus subtilis genome editing using ssDNA with short homology regions

(2012) Yang Wang et al.   NUCLEIC ACIDS RESEARCH

High efficiency recombineering in lactic acid bacteria

(2012) Jan-Peter van Pijkeren et al.   NUCLEIC ACIDS RESEARCH

A Mimicking-of-DNA-Methylation-Patterns Pipeline for Overcoming the Restriction Barrier of Bacteria

(2012) Guoqiang Zhang et al.   PLoS Genetics

MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods

(2011) K. Tamura et al.   MOLECULAR BIOLOGY AND EVOLUTION

Enhancing electro-transformation competency of recalcitrant Bacillus amyloliquefaciens by combining cell-wall weakening and cell-membrane fluidity disturbing

(2010) Guo-qiang Zhang et al.   ANALYTICAL BIOCHEMISTRY

Purification and characterization of a surfactant-stable high-alkaline protease from Bacillus sp. B001

(2010) Aihua Deng et al.   BIORESOURCE TECHNOLOGY

Single-stranded heteroduplex intermediates in λ Red homologous recombination

(2010) Marcello Maresca et al.   BMC MOLECULAR BIOLOGY

Lambda Red Recombineering in Escherichia coli Occurs Through a Fully Single-Stranded Intermediate

(2010) J. A. Mosberg et al.   GENETICS

Transformation of Environmental Bacillus subtilis Isolates by Transiently Inducing Genetic Competence

(2010) Reindert Nijland et al.   PLoS One

Recombineering: a homologous recombination-based method of genetic engineering

(2009) Shyam K Sharan et al.   Nature Protocols

Cre/lox System and PCR-Based Genome Engineering in Bacillus subtilis

(2008) X. Yan et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Efficient point mutagenesis in mycobacteria using single-stranded DNA recombineering: characterization of antimycobacterial drug targets

(2008) Julia C. van Kessel et al.   MOLECULAR MICROBIOLOGY

Analyzing real-time PCR data by the comparative CT method

(2008) Thomas D Schmittgen et al.   Nature Protocols

Recombineering mycobacteria and their phages

(2008) Julia C. van Kessel et al.   NATURE REVIEWS MICROBIOLOGY

DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways

(2008) Zengyi Shao et al.   NUCLEIC ACIDS RESEARCH

Identification and analysis of recombineering functions from Gram-negative and Gram-positive bacteria and their phages

(2008) S. Datta et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA