Article
Chemistry, Analytical
Natalia Olegario dos Santos, Nathalie Ribeiro Wingert, Martin Steppe
Summary: This study aimed to develop and validate an analytical method capable of separating and quantifying S-Rivaroxaban and its enantiomeric impurity R-Rivaroxaban. The Quality by Design (QbD) approach was used to develop a suitable and reproducible method using high-performance liquid chromatography (HPLC). The developed method was validated according to official guidelines, demonstrating selectivity, linearity, precision, accuracy, and sensitivity with a limit of quantification (LOQ) of 0.68 μg/mL for S-Rivaroxaban and 1.0 μg/mL for the chiral impurity. After development and validation, the presence of the R-Rivaroxaban impurity in marketed tablets was determined using the proposed method. This study resulted in the development and validation of a reliable and appropriate chiral analytical method, contributing significantly to quality control routines.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemical Research Methods
Isabelle Fourel
Summary: This article evaluates the chiral selectivity of five polysaccharide-based chiral selectors for the four stereoisomers of five second-generation anticoagulant rodenticides. Different chromatographic parameters were tested to influence the chiral separation, providing suitable combinations for each rodenticide.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Sandra Salido-Fortuna, Maria Luisa Marina, Maria Castro-Puyana
Summary: This study developed a chiral selective method using Electrokinetic Chromatography for the simultaneous separation of licarbazepine acetate and licarbazepine. The method effectively separated the four enantiomers in a short time and showed suitability for various applications.
Article
Chemistry, Analytical
Renu Pandey, Meghan Collins, Xiyuan Lu, Shannon R. Sweeney, Jennifer Chiou, Alessia Lodi, Stefano Tiziani
Summary: The study developed a novel strategy for untargeted chiral metabolomics of hydroxy/amine groups by chiral derivatization coupled with liquid chromatography-high resolution tandem mass spectrometry (LC-HR-MS/MS). This method successfully enabled the simultaneous measurement of different classes of enantiomeric metabolites in a single analytical run, providing new insights in the detection of disease biomarkers.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Milada Vitova, Milena Stranska, Andrea Palyzova, Tomas Rezanka
Summary: Cardiolipins are widely present in various organisms, and significant differences in molecular species were found among different organisms. The cleavage products of CLs also varied between organisms.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Imad A. Haidar Ahmad, Alaina Kiffer, Rodell C. Barrientos, Gioacchino Luca Losacco, Andrew Singh, Vladimir Shchurik, Heather Wang, Ian Mangion, Erik L. Regalado
Summary: Tandem column liquid chromatography is a convenient and cost-effective method, but its application is limited by the selection of suitable column combinations. To overcome this challenge, we propose a straightforward in silico approach to map the separation landscape of multiple tandem columns and eluent combinations.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Yazhou Shuang, Tianci Zhang, Hui Zhong, Laisheng Li
Summary: A new high-throughput LC-MS/MS method was developed for convenient monitoring of chiral pesticides in fruits and vegetables, enabling simultaneous enantio-separation of multiple analytes and simplification of sample pre-treatment. This method demonstrated good linearity, high recoveries, and accurate determination of enantiomeric fractions in strawberry, cucumber, and tomato samples.
Article
Biochemical Research Methods
Andrea Palyzova, Irina A. Guschina, Tomas Rezanka
Summary: In this study, G3P and G1P were detected in four bacterial strains using GC/MS and LC/MS methods. Different analytical techniques and chromatographic modes were employed to confirm that PG is the most abundant phospholipid in these bacteria.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Review
Pharmacology & Pharmacy
Jae Geun Song, Kshitis Chandra Baral, Gyu-Lin Kim, Ji-Won Park, Soo-Hwa Seo, Da-Hyun Kim, Dong Hoon Jung, Nonye Linda Ifekpolugo, Hyo-Kyung Han
Summary: The application of therapeutic proteins in pharmaceuticals is expanding, and efficient bioanalytical methods are crucial for their identification and clinical development. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is often used for quantitative protein analysis in complex samples due to its sensitivity, specificity, and throughput. Proper sample preparation is important to minimize interference and enhance the accuracy of LC-MS/MS assays.
Article
Chemistry, Analytical
Jia Lun, Siman Ma, Mengyao Xue, Pengfei Zhao, Yongbo Song, Xingjie Guo
Summary: This study presents a method for the simultaneous quantification of five proton pump inhibitors at enantiomeric levels in environmental soil and river sediment using chiral high performance liquid chromatography-tandem mass spectrometry. The method achieved baseline separation of the enantiomers, low detection and quantification limits, and good recoveries. Through systematic optimization of sample preparation and experimental parameters, the method demonstrated strong practical value.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Rawiwan Wattanayon, Barbara Kasprzyk-Hordern
Summary: This study presented a new analytical method for the comprehensive profiling of a wide range of antifungal agents and their metabolites in the environment. The majority of antifungal agents were found in aqueous environments, sourced from animal and plant protection rather than human usage. Chiral fungicides in river water were enriched with a single enantiomer, raising potential ecological implications requiring further investigation.
ANALYTICAL METHODS
(2021)
Review
Chemistry, Analytical
Tianyu Zhou, Hongji Li, Mengxiang Shang, Dongshu Sun, Chunbo Liu, Guangbo Che
Summary: This review comprehensively summarizes recent detection methods for riboflavin in food, biological and pharmaceutical fields, including mass spectrometry, fluorescent detection, electrochemical detection, immunoassay, etc. Customized detection mechanisms for riboflavin structure/physicochemical-property and the development of new functional materials have led to excellent detection performances with high sensitivity, precision, and accuracy.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Zhijun Zhu, Shuling Xu, Zicong Wang, Daniel G. Delafield, Michael J. Rigby, Gaoyuan Lu, Ting-Jia Gu, Peng-Kai Liu, Min Ma, Luigi Puglielli, Lingjun Li
Summary: This study developed a quantitative strategy called CHRISTMAS, which utilizes l-DiLeu and d-DiLeu labeling to achieve absolute quantitation of d-amino acids and l-amino acids in complex biological samples. This strategy improves accuracy and sensitivity, and provides valuable insights into the involvement of d-amino acids in aging, AD progression, and neurodegeneration.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Wei Luo, Jiaxin Pan, Bo Chen, Ming Ma
Summary: A paper spray tandem mass spectrometric (PS-MS/MS) method without column separation was developed for the rapid screening of clonidine in dietary supplements. PS-MS/MS provided acceptable performance compared to high-performance liquid chromatography - tandem mass spectrometry (HPLC-MS/MS) in terms of limits of detection and quantification, accuracy, and precision. Internal standards including nizatidine, tizanidine, and apraclonidine were investigated to compensate for the ionization suppression of complex plant-based pharmaceutical products.
ANALYTICAL LETTERS
(2023)
Article
Food Science & Technology
Yan Zhu, Lei Di, Shengyu Cheng, Yueying Jin, Minghui Zhang, Lingli Zhang, Xin Wang, Xi-Ling Li, Yu Han, Chun-Yan Xu, Jun Zhe Min
Summary: A novel UHPLC-MS/MS method using the CTOD probe was developed for the simultaneous detection and chiral resolution of DL-amino acids in beer. The method showed high sensitivity and selectivity, with LOD and LOQ values ranging from 0.05 to 0.50 pmol. The method was applied to the analysis of DL-amino acids in beer samples and revealed that the beers contained both D-amino acids and L-amino acids, with the average content of L-amino acids being significantly higher than that of D-amino acids.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)