Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1648, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2021.462185
Keywords
Enantiomeric separation; Enzymatic hydrolysis; RP-HPLC/MS-ESI+; Cardiolipins; Bacterium; Yeast; Alga; Spinach; Bovine heart
Funding
- Czech Science Foundation (GACR) [1826463S]
- Ministry of Education, Youth and Sports of the Czech Republic
- program Interreg VA: grant REEgain [ATCZ172]
- [RVO61388971]
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Cardiolipins are widely present in various organisms, and significant differences in molecular species were found among different organisms. The cleavage products of CLs also varied between organisms.
Cardiolipins (1,3-bis(sn-3'-phosphatidyl)-sn-glycerol) (CLs) are widespread in many organisms, from bacteria to higher green plants and mammals. CLs were observed in Gram-positive bacterium of the genus Kocuria, brewer's yeast Saccharomyces, the green alga Chlamydomonas, spinach and beef heart. A mixture of molecular species of CLs was obtained from total lipids by hydrophilic interaction liquid chromatography (HILIC), and these were further separated and identified by reversed phase LC/MS with negative tandem electrospray ionization. The majority of CLs molecular species from each organism were cleaved using phospholipase C from Bacillus cereus. This phospholipase cleaves CLs into 1,2-diglycerols and phosphatidylglycerol 3-phosphates, which were then separated. After CLs cleavage, diacylglycerols such as sn-1,2-diacyl-3-acetyl-glycerols (i.e., triacylglycerols) were separated and identified by chiral chromatography/MS-positive tandem ESI. Significant differences in the composition of the molecular species between the 3-(3-sn-phosphatidyl) and 1-(3-sn-phosphatidyl) moieties of CLs were found in all organisms tested. Molecular species of CLs that contained four different fatty acids were identified in all five samples, and CLs containing very long chain fatty acids were identified in yeast. In addition, CLs containing both enantiomers (at the sn-2 carbon) were present in the bacterium tested. These findings were further supported by data already published in GenBank where, in the same family - Micrococ-caceae - both enzymes responsible for chirality in the sn-2 position, glycerol-3-phosphate and glycerol-1-phosphate dehydrogenases, were present. (C) 2021 Elsevier B.V. All rights reserved.
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