Chemical synthesis and biophysical applications of membrane proteins

Traceless Preparation of C-Terminal α-Ketoacids for Chemical Protein Synthesis by α-Ketoacid-Hydroxylamine Ligation: Synthesis of SUMO2/3

(2014) Thomas G. Wucherpfennig et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Total chemical synthesis of a membrane protein domain analogue containing two transmembrane helices: functional reconstitution of the semisynthetic sensory rhodopsin/transducer complex

(2014) Marc Dittmann et al.   JOURNAL OF PEPTIDE SCIENCE

Expedient Total Synthesis of Small to Medium-Sized Membrane Proteins via Fmoc Chemistry

(2014) Ji-Shen Zheng et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A New Method for Synthesis of Peptide Thioesters via Irreversible N-to-S Acyl Transfer

(2014) Ji-Shen Zheng et al.   ORGANIC LETTERS

Development of New Thioester Equivalents for Protein Chemical Synthesis

(2013) Ji-Shen Zheng et al.   ACCOUNTS OF CHEMICAL RESEARCH

Chemoenzymatic Synthesis of the Immunoglobulin Domain of Tim-3 Carrying a Complex-Type N-Glycan by Using a One-pot Ligation

(2013) Yuya Asahina et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Synthesis of Autophagosomal Marker Protein LC3-II under Detergent-Free Conditions

(2013) Yi-Chao Huang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Molecular signatures of G-protein-coupled receptors

(2013) A. J. Venkatakrishnan et al.   NATURE

Chemical synthesis of proteins using peptide hydrazides as thioester surrogates

(2013) Ji-Shen Zheng et al.   Nature Protocols

Semisynthetic K+ channels show that the constricted conformation of the selectivity filter is not the C-type inactivated state

(2013) P. K. Devaraneni et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Using protein backbone mutagenesis to dissect the link between ion occupancy and C-type inactivation in K+ channels

(2013) K. Matulef et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Convergent Chemical Synthesis of Proteins by Ligation of Peptide Hydrazides

(2012) Ge-Min Fang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Membrane Protein Structure and Dynamics from NMR Spectroscopy

(2012) Mei Hong et al.   Annual Review of Physical Chemistry

Native chemical ligation in dimethylformamide can be performed chemoselectively without racemization

(2012) Marc Dittmann et al.   JOURNAL OF PEPTIDE SCIENCE

Revisiting lab-on-a-chip technology for drug discovery

(2012) Pavel Neužil et al.   NATURE REVIEWS DRUG DISCOVERY

Total Chemical Synthesis of an Integral Membrane Enzyme: Diacylglycerol Kinase fromEscherichia coli

(2011) Sunanda Lahiri et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Protein Chemical Synthesis by Ligation of Peptide Hydrazides

(2011) Ge-Min Fang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Chemical Synthesis of Integral Membrane Proteins: Methods and Applications

(2011) Fei Shen et al.   ISRAEL JOURNAL OF CHEMISTRY

On the Synthesis of Conformationally Modified Peptides through Isonitrile Chemistry: Implications for Dealing with Polypeptide Aggregation

(2011) Xiangyang Wu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Fmoc Synthesis of Peptide Thioesters without Post-Chain-Assembly Manipulation

(2011) Ji-Shen Zheng et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

NMR Detection of pH-Dependent Histidine–Water Proton Exchange Reveals the Conduction Mechanism of a Transmembrane Proton Channel

(2011) Fanghao Hu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Overcoming barriers to membrane protein structure determination

(2011) Roslyn M Bill et al.   NATURE BIOTECHNOLOGY

Rational development of a strategy for modifying the aggregatibility of proteins

(2011) Z. Tan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Hexafluoro-2-propanol as a potent cosolvent for chemical ligation of membrane proteins

(2011) Fei Shen et al.   Science China-Chemistry

Chemical Protein Synthesis by Kinetically Controlled Ligation of Peptide O-Esters

(2010) Ji-Shen Zheng et al.   CHEMBIOCHEM

Native chemical ligation of hydrophobic peptides in organic solvents

(2010) Marc Dittmann et al.   JOURNAL OF PEPTIDE SCIENCE

G protein-coupled receptors: novel targets for drug discovery in cancer

(2010) Rosamaria Lappano et al.   NATURE REVIEWS DRUG DISCOVERY

Chemical synthesis of mouse pro-opiomelanocortin(1–74) by azido-protected glycopeptide ligation via the thioester method

(2010) Hidekazu Katayama et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Bis(2-sulfanylethyl)amino Native Peptide Ligation

(2010) Nathalie Ollivier et al.   ORGANIC LETTERS

PeptidylN,N-Bis(2-mercaptoethyl)-amides as Thioester Precursors for Native Chemical Ligation†

(2010) Wen Hou et al.   ORGANIC LETTERS

Mechanisms of Proton Conduction and Gating in Influenza M2 Proton Channels from Solid-State NMR

(2010) F. Hu et al.   SCIENCE

Synthesis of the sphingolipid activator protein, saposin C, using an azido-protected O-acyl isopeptide as an aggregation-disrupting element

(2010) Hironobu Hojo et al.   TETRAHEDRON LETTERS

Modular Strategy for the Semisynthesis of a K+Channel: Investigating Interactions of the Pore Helix

(2009) Alexander G. Komarov et al.   ACS Chemical Biology

Biophysical dissection of membrane proteins

(2009) Stephen H. White   NATURE

The structure and function of G-protein-coupled receptors

(2009) Daniel M. Rosenbaum et al.   NATURE

An Efficient Fmoc-SPPS Approach for the Generation of Thioester Peptide Precursors for Use in Native Chemical Ligation

(2008) Juan B. Blanco-Canosa et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Large-scale production of functional membrane proteins

(2008) F. Junge et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Total chemical synthesis of proteins

(2008) Stephen B. H. Kent   CHEMICAL SOCIETY REVIEWS

Chemical synthesis and semisynthesis of membrane proteins

(2008) Diana Olschewski et al.   Molecular BioSystems

Application of a novel thioesterification reaction to the synthesis of chemokine CCL27 by the modified thioester method

(2008) Hironobu Hojo et al.   ORGANIC & BIOMOLECULAR CHEMISTRY