Article
Chemistry, Physical
Daijun Zheng, Yasuhisa Asano
Summary: A biocatalyst with selective enantioselectivity was developed through mutagenesis on aldoxime dehydratase derived from Pseudomonas chlororaphis B23, resulting in the synthesis of chiral compounds.
Article
Engineering, Chemical
Linsong Yang, Daozhu Xu, Luyao Jiang, Yucai He
Summary: Efficient bioreduction of COBE to (R)-CHBE was achieved using a biocompatible organic solvent-deep eutectic solvent-water reaction medium. A high (R)-CHBE yield was obtained through whole-cell biocatalysis, demonstrating potential application in future pharmaceutical synthesis.
Article
Chemistry, Physical
Chuanhua Zhou, Xi Chen, Tong Lv, Xu Han, Jinhui Feng, Weidong Liu, Qiaqing Wu, Dunming Zhu
Summary: In this study, a unique halohydrin dehalogenase (AbHheG) was found to have high activity and alpha/beta-regioselectivity toward the ring opening of racemic styrene oxide with cyanate but with poor stereoselectivity. By reshaping the substrate-binding site of AbHheG, a variant Y15M/N182S was obtained with excellent alpha/beta-regioselectivity and stereoselectivity. This work demonstrated that halohydrin dehalogenase is a useful biocatalyst for the synthesis of both enantiomers of 4-aryl-2-oxazolidinones from readily available racemic styrene oxides.
Article
Chemistry, Medicinal
Divya Chauhan, Syad Riaz Hashim, Prinka Rani, Sushil Kumar, Navratan Shrimal, Divya Shastri, Vinit Raj
Summary: A novel series of 4H-chromene derivatives were designed and synthesized for the treatment of cancer and related diseases. Compounds A4 and A9 showed significant anticancer activity against HT-29 cells, while compound A9 exhibited promising anticonvulsant effects.
MEDICINAL CHEMISTRY
(2021)
Article
Thermodynamics
Sana Enomoto, Miyu Umeda, Taro Yamamoto, Ewa Juszynska-Galazka, Yuji Miyazaki, Hal Suzuki
Summary: The polymorphism and thermodynamic properties of 4-cyano-3-fluorophenyl 4-pentylbenzoate were studied using adiabatic calorimetry, differential scanning calorimetry (DSC), simultaneous X-ray diffractometry and DSC (XRD-DSC), and polarized optical microscopy (POM). Seven phases were identified, including a nematic phase, glass of the nematic phase, and four crystal phases. The thermodynamic stabilities of the phases were determined, and solid-solid phase transitions and crystallization kinetics were observed.
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
(2023)
Article
Thermodynamics
Jinyi Chen, Hongxing Wang
Summary: This study obtained the density, viscosity, and saturated vapor pressure data of pure 3-chloro-4-fluoronitrobenzene and 3-chloro-2-fluoronitrobenzene under specific temperature and pressure conditions. The experimental results show that there is a linear relationship between temperature and density, viscosity data can be described by multiple equations, and the relationship between saturated vapor pressure and temperature can be accurately described by two equations.
JOURNAL OF CHEMICAL THERMODYNAMICS
(2021)
Article
Biochemistry & Molecular Biology
Matthew K. Craft, Grover L. Waldrop
Summary: The rise of antibacterial-resistant bacteria is a major problem globally, requiring novel antibacterial agents to combat. Acetyl-CoA carboxylase (ACC) is a validated target, and SABA1 represents a potentially new class of antibiotics that can be used to combat the antibacterial resistance crisis.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2022)
Article
Spectroscopy
Jelena M. Ladarevic, Bojan D. Bozic, Vesna D. Vitnik, Luka R. Matovic, Dusan Z. Mijin, Zeljko J. Vitnik
Summary: In order to improve the performance of theoretical UV-Vis spectra predictions, a study of solvatochromic properties of ten azo pyridone dyes was conducted. By applying the concept of linear solvation energy relationships using Kamlet-Taft and Catalan models, the intermolecular solvent-solute interactions were quantitatively estimated. Theoretical UV-Vis spectra were calculated using four TD-DFT methods in nine different solvents, and new polylinear equations for more accurate prediction of UV-Vis maxima were developed.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Kabir M. Uddin, Mohiuddin Sakib, Siam Siraji, Riaz Uddin, Shofiur Rahman, Abdullah Alodhayb, Khuloud A. Alibrahim, Ajoy Kumer, M. Mahbubul Matin, Md. Mosharef H. Bhuiyan
Summary: In this study, two novel series of derivatives (1-6) were synthesized using microwave-assisted Knoevenagel condensation. The synthesized compounds were characterized and evaluated for their pharmacodynamics, toxicity profiles, and biological activities. In silico studies, including prediction of activity spectra for substances (PASS) and absorption, distribution, metabolism, excretion, and toxicity (ADMET) studies, were conducted. Molecular docking and molecular dynamics (MD) simulation were employed to investigate the binding mode, interaction sites, and thermodynamic stability of the derivatives. Compound 3 showed promising potential as a new anticancer and antiparasitic drug, but further research is needed to validate its efficacy.
Article
Chemistry, Organic
Alina N. Grozav, Sergiy V. Kemskiy, Mariana Z. Fedoriv, Vitaliy A. Chornous, Alina A. Palamar, Viktor I. Dorokhov, Eduard B. Rusanov, Mikhailo V. Vovk
Summary: Ethyl 5-chloro-4-(2-nitroethenyl)pyrrole-3-carboxylates were synthesized from the condensation of ethyl 5-chloro-4-formylpyrrole-3-carboxylates with nitromethane. These compounds reacted with N-methylazomethine ylide to form ethyl 5-chloro-4-(4-nitropyrrolidin-3-yl)pyrrole-3-carboxylates. Hydrolysis and reductive cyclization of these compounds led to the synthesis of hexahydrodipyrrolo[3,4-b:3',4'-d]pyridin-5(1H)-one via intermediate ethyl 4-(4-aminopyrrolidin-3-yl)-5-chloropyrrole-3-carboxylate derivatives.
CHEMISTRY OF HETEROCYCLIC COMPOUNDS
(2022)
Letter
Chemistry, Organic
Marta Romaniszyn, Anna Skrzynska, Joanna Dybowska, Lukasz Albrecht
Summary: A novel organocatalytic reaction cascade has been described for the stereocontrolled functionalization of cyanocoumarins, producing biologically active coumarin and delta-lactone-fused products.
Article
Spectroscopy
Gi Woo Lee
Summary: This study focused on investigating the formation of 3-chloro-4-fluorobenzyl and 4-fluorobenzyl radicals through experimental observation and theoretical calculations. The vibrational spectrum and transition energies of 3-chloro-4-fluorobenzyl radical were analyzed and determined with Franck-Condon simulation and density functional theory calculations.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Spectroscopy
Yesim S. Kara, Mustafa Unsal, Nalan Tekin, Asli Esme
Summary: In this study, two novel oxadiazole derivatives were synthesized and their relationship with solvent effects was investigated using experimental and theoretical analyses. The linear solvation energy relationships showed better correlation with the vibrational frequencies of the compounds than other empirical solvent parameters. Additionally, the quadratic equation provided more accurate predictions for the vibrational frequency locations compared to the Swain and linear solvation energy relationships equations.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Organic
Sanjay Singh, Ravi Saini, Ravi P. Singh
Summary: An unprecedented organocatalyzed asymmetric vinylogous Michael reaction with excellent enantiomeric ratio and yield has been reported. The remarkable selectivity is attributed to the hydrogen bonding ability of the catalyst. The reaction is versatile and practical for the synthesis of various gamma-functionalized coumarin derivatives.
JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Kamila Buzun, Anna Kryshchyshyn-Dylevych, Julia Senkiv, Olexandra Roman, Andrzej Gzella, Krzysztof Bielawski, Anna Bielawska, Roman Lesyk
Summary: A series of novel 5-[(Z,2Z)-2-chloro-3-(4-nitrophenyl)-2-propenylidene]-thiazolidinones were synthesized, with compound 2h showing the highest level of antimitotic activity against various cancer cell lines. Hit compounds were found to have low toxicity towards normal human blood lymphocytes and a fairly wide therapeutic range. The significance of specific substituents in the 4-thiazolidinone derivatives for anticancer cytotoxicity levels was established.
Review
Biotechnology & Applied Microbiology
Kuo Zhao, Heng Tang, Bo Zhang, Shuping Zou, Zhiqiang Liu, Yuguo Zheng
Summary: Vitamin B5, also known as D-pantothenic acid (D-PA), is a vital micronutrient with significant physiological functions. It is widely used in various fields such as food, medicine, feed, and cosmetics. This review provides an introduction to the role and applications of D-PA, summarizes its biosynthesis pathways and enzymes, analyzes strategies for excessive D-PA production through cell factory development, and discusses the prospects of microbial production of D-PA.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yu-Qi Dong, Ji-Dong Shen, Long Pan, Ji-Hong Huang, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: Fructose, a crucial precursor for rare sugar biosynthesis, was produced by thermophilic glucose isomerases (GI) from Caldicellulosiruptor acetigenus (CAGI), Thermoanaerobacter thermocopriae (TTGI), and Thermotoga petrophila (TPGI). The optimal pH for CAGI and TTGI was found to be 8.0 and maintained at 80% activity in a slightly acidic environment. After 24 hours of incubation at 90 degrees Celsius, the relative residual activities of CAGI and TTGI were 40.6% and 52.6%, respectively. Furthermore, CAGI and TTGI exhibited high catalytic performance with conversion rates of 54.2% and 54.1% after an hour at 85 degrees Celsius with 200 g/L glucose. This study provides valuable enzyme candidates for fructose production in the industry with significant cost reduction.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Zhen-Yang Shen, Yi-Feng Wang, Ying Wang, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: This study investigates the metabolic response and crucial genes in the biosynthetic pathway of iota-methionine. The relationship between different pathways and their controlling forces on metabolic flux are also examined. The findings provide insights into the biosynthesis of iota-methionine and offer a new research method for synthesizing other metabolic products in multilevel regulated and multibranched biosynthetic pathways.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Xue Cai, Xue Shi, Si-Qi Liu, Yu Qiang, Ji -Dong Shen, Bo Zhang, Zhi-Qiang Liu, Yu -Guo Zheng
Summary: In this study, a hot spot analysis was conducted on the ketopantoate hydroxymethyltransferase from Corynebacterium glutamicum, resulting in an increased production of D-PA. The double-site variant CgKPHMT-K25A/E189S exhibited higher enzyme activity and catalytic efficiency, leading to a significant increase in D-PA yield.
JOURNAL OF BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Haonan Wang, Xia Ke, Rui Jia, Lianggang Huang, Zhiqiang Liu, Yuguo Zheng
Summary: In this study, a high-yield GA(3) producing strain of Fusarium fujikuroi was constructed using metabolic modification, transcriptome analysis, and promoter engineering. Over-expression of AreA and Lae1 genes resulted in an initial strain with GA(3) production of 2.78 g L-1. By employing a nitrogen-responsive bidirectional promoter, the production of GA(3) was further increased to 3.02 g L-1. The top 20 upregulated genes in GA(3) over-production were characterized, providing potential targets for further strain development. This work offers an efficient microbial development platform for industrial GA(3) production.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Kun Niu, Yu-Xin Qi, Hong-Wei Cai, Yi-Xin Ye, Hai-Yan Zhou, Xiao-Tian Liu, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: The effects of different amino acids on Echinocandin B biosynthesis were investigated. It was found that adding L-threonine did not significantly increase the fermentation titer of Echinocandin B. However, using methyl oleate as a carbon source resulted in a two-fold increase in Echinocandin B production. Transcription level analysis indicated that the gene an655543 related to L-threonine biosynthesis had high expression during fermentation, suggesting that exogenous addition of L-threonine had minimal impact. Additionally, the transcription level of gene ecdA appeared to be the main limiting factor for Echinocandin B biosynthesis. This study provides a foundation for future modifications of Echinocandin B-producing strains.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Han-Lin Liu, Jia-Min Wu, Xin-Tong Deng, Lan Yu, Pu-Hong Yi, Zhi-Qiang Liu, Ya-Ping Xue, Li-Qun Jin, Yu-Guo Zheng
Summary: This study developed a biocatalytic cascade for the production of PPO with high efficiency and low cost. The cascade integrated different enzymes and showed superior catalytic efficiency in the synthesis of PPO from d,l-PPT. It achieved high space-time yield and complete conversion of the substrate.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Zhong-Ce Hu, Chui-Mu Zheng, Yun-Chao Tao, Shu-Nan Wang, Yuan-Shan Wang, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: This study aimed to increase the accumulation of S-adenosyl-L-methionine (SAM) in Saccharomyces cerevisiae by promoting ATP availability. The results showed that the lack of gene sod1 benefited ATP generation and positively regulated SAM synthesis. Additionally, improving substrate assimilation further enhanced SAM production. The SAM concentration of strain Delta SOD1 was increased from 7.3 g L-1 to 10.1 g L-1 in a 5-L fermenter.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Fang-Ying Zhu, Qing Yang, Min Cao, Ken Zheng, Xiao-Jian Zhang, Qi Shen, Xue Cai, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: D-(-)-Pantolactone (DPL) is an important intermediate for producing D-(+)-pantothenate (vitamin B5). In this study, a newly discovered L-pantolactone dehydrogenase (RhoLPLDH) was used for the oxidation of L-(+)-pantolactone (LPL) to ketopantoyl lactone (KPL). Strategies involving chaperone assistance and decreasing induction temperature were employed to achieve soluble expression of RhoLPLDH in E. coli. Directed evolution and hydrophilicity-based engineering were used to enhance the activity and thermostability of RhoLPLDH. A two stages in one-pot method was successfully used to deracemize 1 M D,L-PL with a yield of 91.2%. These results demonstrate the potential of the CM5 catalyst for enzyme cascade deracemization in the production of DPL.
JOURNAL OF BIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Bo Zhang, Hui Yang, Zidan Wu, Jiayuan Pan, Shirong Li, Lifeng Chen, Xue Cai, Zhiqiang Liu, Yuguo Zheng
Summary: Gene expression in spatiotemporal distribution improves the ability of cells to respond to changing environments. A genetic circuit based on the Esa quorum sensing and lac operon was designed to achieve dynamic temporal gene expression. The circuit was also applied in microbial cell factories to enhance the production of various compounds, including L-cysteine, pantothenic acid, methionine, homoserine, and 2-aminobutyric acid, resulting in increased yields.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Zhen-Yang Shen, Yi-Feng Wang, Li-Juan Wang, Ying Wang, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: In this study, the availability of 5-methyl-tetrahydrofolate was identified as the main limitation in the biosynthesis of L-methionine. The one-carbon unit cycle was thoroughly investigated and modified to enhance the production of L-methionine. The final strain MYA/pAmFA-4 achieved the highest reported titer of 20.89 g/L L-methionine. This study provides insights for the biosynthesis of other metabolites with complex multibranched pathways or requiring one-carbon units.
Article
Biochemistry & Molecular Biology
Zhong-Ce Hu, Yun-Chao Tao, Jun-Chao Pan, Chui-Mu Zheng, Yuan-Shan Wang, Ya-Ping Xue, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: A mutant strain of Saccharomyces cerevisiae with increased S-adenosyl-l-methionine (SAM) production was successfully generated through UV mutagenesis and high-throughput selection. The mutant strain exhibited a higher SAM yield and altered gene expression compared to the parent strain. In a 5-L fermenter, the mutant strain produced 10.92 ± 0.2 g/L SAM after 96 h of fermentation, showing a 2.02-fold increase in product yield. This work provides a promising foundation for industrial production of SAM.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Jian-Miao Xu, Wei-Jie Wang, Zhuo-Ting Chen, Yuan-Yuan Zhou, Jia-Jia Pan, Feng Cheng, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: In this study, Bacillus cereus was isolated from soil and used for repairing concrete cracks by evaluating the effects of four different thickeners at varying concentrations. The most effective result was achieved with 10 g/L of sodium carboxymethyl cellulose (CMC-Na). The study also assessed the quantity and sizes of crystals generated during the bacterial mineralization process to improve understanding of the process.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Hui Yang, Bo Zhang, Zi-Dan Wu, Li-Feng Chen, Jia-Yuan Pan, Xiao-Ling Xiu, Xue Cai, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: This study proposed a combinatorial metabolic engineering strategy to develop an efficient Escherichia coli cell factory for L-cysteine production. The researchers investigated the crucial regulatory modes and employed multi-copy gene expression to achieve a two-stage fermentation for improved balance between production and growth. They further optimized carbon flux distribution by modifying the C1 unit metabolism and the glycolytic pathway, and demonstrated the superior performance of thiosulfate utilization pathways in enhancing L-cysteine production. The work also highlighted the vital role of synergistic enhancement of sulfur-carbon metabolism in L-cysteine overproduction. In a 5 L bioreactor, the engineered strain accumulated 12.6 g/L of L-cysteine.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Hui Yang, Bo Zhang, Zi-Dan Wu, Li-Feng Chen, Jia-Yuan Pan, Xiao-Ling Xiu, Xue Cai, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: This study presents a combinatorial metabolic engineering strategy for the development of an efficient Escherichia coli cell factory dedicated to L-cysteine production. By optimizing crucial regulatory modes, improving the balance between production and growth, optimizing carbon flux distribution, and modifying sulfur assimilation pathways, the study successfully achieved high L-cysteine titer.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)