Article
Biotechnology & Applied Microbiology
Madhan Jeyaraman, Shiva Kumar Bingi, Sathish Muthu, Naveen Jeyaraman, Rathinavelpandian Perunchezhian Packkyarathinam, Rajni Ranjan, Shilpa Sharma, Saurabh Kumar Jha, Manish Khanna, Sree Naga Sowndary Rajendran, Ramya Lakshmi Rajendran, Prakash Gangadaran
Summary: Human bone marrow is a promising source of mesenchymal stromal cells (MSCs) for regenerative procedures involving cartilage and bone. However, the low proportion of MSCs in the bone marrow requires careful harvesting and processing techniques. There is currently a lack of consensus regarding the variables in the harvesting and processing technique of bone marrow aspiration concentrate (BMAC) and their impact on MSC yield.
BIOENGINEERING-BASEL
(2022)
Article
Cell Biology
Wu Duan, Huajie Zou, Nan Zang, Dongxia Ma, Bo Yang, Lin Zhu
Summary: Bone marrow adipose tissue (MAT) can have both local and systemic effects on metabolic homeostasis. The effects of metformin, a first-line drug for type 2 diabetes, on MAT and bone marrow mesenchymal stem cell (BM-MSC) differentiation are conflicting. In vivo and in vitro experiments showed that metformin increased MAT in mice tibia and promoted both osteogenic and lipogenic gene expression in mouse bone marrow. However, metformin inhibited lipogenesis and promoted osteogenesis of MSC in vitro. The increased MAT may be attributed to the filling of adipose tissue after metformin-induced apoptosis of bone marrow stromal cells.
Article
Cell & Tissue Engineering
Behnoosh Tayebi, Mahnaz Babaahmadi, Mohammad Pakzad, Mostafa Hajinasrollah, Farhad Mostafaei, Shahrbanoo Jahangiri, Amir Kamali, Hossein Baharvand, Mohamadreza Baghaban Eslaminejad, Seyedeh-Nafiseh Hassani, Ensiyeh Hajizadeh-Saffar
Summary: Mesenchymal stromal cells (MSCs) have shown potential in treating inflammatory and non-inflammatory diseases, but their clinical applications require strict evaluations and monitoring to ensure compliance with safety standards. This study aimed to assess the potential toxicity effects of cryopreserved human bone marrow-derived clonal MSCs in animal models during different time periods.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Oncology
Rafaela Rossetti, Felipe Augusto Ros, Lucas Eduardo Botelho de Souza, Juliana de Matos Maconetto, Pericles Natan Mendes da Costa, Fernanda Ursoli Ferreira, Josiane Serrano Borges, Julianne Vargas de Carvalho, Nayara Patricia Morotti, Simone Kashima, Dimas Tadeu Covas
Summary: The bone marrow microenvironment may contain two distinct populations of mesenchymal stromal cells (MSC), one located perivascularly and the other in the endosteum. Recent research has focused on better understanding these MSC subsets in order to improve their clinical applications. Studies indicate that compact bone is a rich source of MSC and offers lower risk of contamination by hematopoietic cells. Compact bone-derived MSC also show higher osteogenic potential compared to bone marrow-derived MSC. These findings suggest the presence of functionally different MSC populations in bone marrow and compact bone.
EXPERIMENTAL CELL RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Yujie Song, Christian Jorgensen
Summary: This review highlights the potential of mesenchymal stromal cells (MSCs) in treating osteoarthritis (OA), focusing on their chondrogenic and immunomodulatory potential. The review provides an overview of the effects of MSCs in preclinical and clinical studies of OA treatment, comparing different MSC sources. Furthermore, the review summarizes the current evidence for the assessment of cartilage in OA using magnetic resonance imaging (MRI) and second-look arthroscopy after MSC therapy.
Article
Immunology
Fulvio Massaro, Florent Corrillon, Basile Stamatopoulos, Nathan Dubois, Achille Ruer, Nathalie Meuleman, Dominique Bron, Laurence Lagneaux
Summary: Mesenchymal stromal cells (MSC) play a critical role in aging and inflammaging, and studying them is important for understanding the mechanisms of diseases in the elderly.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Health Care Sciences & Services
Suncica Kapor, Milica Vukotic, Tijana Suboticki, Dragoslava Dikic, Olivera Mitrovic Ajtic, Milica Radojkovic, Vladan P. Cokic, Juan F. Santibanez
Summary: The study found that HU inhibits the growth of BMMSC, causing senescence-like changes and affecting cellular differentiation and immunoregulatory functions.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Chiara Valsecchi, Stefania Croce, Alice Maltese, Lorenza Montagna, Elisa Lenta, Alice Nevone, Maria Girelli, Paolo Milani, Tiziana Bosoni, Margherita Massa, Carlotta Abba, Rita Campanelli, Jessica Ripepi, Annalisa De Silvestri, Adriana Carolei, Giovanni Palladini, Marco Zecca, Mario Nuvolone, Maria Antonietta Avanzini
Summary: Immunoglobulin light-chain amyloidosis (AL) is characterized by misfolded light chains, and this study aimed to characterize bone marrow (BM)-MSCs from AL patients. The results showed that AL-MSCs were comparable to those from healthy donors in morphology and differentiation capacities, but had a reduced proliferation rate compared to healthy donor MSCs. Additionally, the modulatory effect of AL-MSCs on plasma cells was similar to that of healthy donor MSCs.
Article
Cell & Tissue Engineering
Maiko Sezaki, Subinoy Biswas, Sayuri Nakata, Motohiko Oshima, Shuhei Koide, Nicole Pui Yu Ho, Nobukazu Okamoto, Takeshi Miyamoto, Atsushi Iwama, Hitoshi Takizawa
Summary: This study characterizes the heterogeneity of hMSCs and their ability to efficiently form humanized ossicles in good donors by correlating with the frequency and functionality of chondrocyte progenitors. CD271(+)CD51(+) stromal cell subset was found to possess enhanced hMSC activity, with PALLADIN-negative cells displaying the greatest CFU-F potential in subsets enriched for greater chondrogenic ability. The findings contribute to better understanding and isolation of ossicle-forming hMSCs for optimized engineering of human bone organs.
STEM CELLS AND DEVELOPMENT
(2021)
Review
Cell Biology
Kevin Woods, Borhane Guezguez
Summary: Mesenchymal stromal cells (MSCs) are a diverse cell population known for their nutrient-producing and immunomodulatory functions, crucial in regulating hematopoietic stem cells (HSC) in the bone marrow for blood production and immunity. MSCs contribute to bone integrity and hormone supply by differentiating into osteoblasts and adipocytes, playing a key role in the complex interactions of the HSC niche.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Kevin Woods, Borhane Guezguez
Summary: MSCs are a heterogeneous cell population found in various tissues in the body, with important regulatory functions, especially in the bone marrow where they play a critical role in regulating HSCs. They contribute to maintaining bone integrity and hormonal nutrition supply, as well as regulating the homeostasis and differentiation of the HSC niche through differentiation into different cell types. Research on MSCs helps understand their role in regulating cell interactions and homeostasis in the body.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Sarah Hochmann, Kristy Ou, Rodolphe Poupardin, Michaela Mittermeir, Martin Textor, Salaheddine Ali, Martin Wolf, Agnes Ellinghaus, Dorit Jacobi, Juri A. J. Elmiger, Samantha Donsante, Mara Riminucci, Richard Schaefer, Uwe Kornak, Oliver Klein, Katharina Schallmoser, Katharina Schmidt-Bleek, Georg N. Duda, Julia K. Polansky, Sven Geissler, Dirk Strunk
Summary: Multipotent stromal cells are attractive sources for cell therapy and tissue engineering, but their application is limited due to functional diversity. In this study, we compared the regenerative potential of different stromal cell types in vitro and in vivo and found that only bone marrow stromal cells (BMSCs) could fully regenerate critical-size femoral defects. This ability was attributed to cell type-specific epigenetic landscapes controlling transcriptional stromal differentiation networks. BMSCs initiated defect healing and eventually contributed to tissue remodeling.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Article
Oncology
Zsolt Matula, Gabor Mikala, Szilvia Lukacsi, Janos Matko, Tamas Kovacs, Eva Monostori, Ferenc Uher, Istvan Valyi-Nagy
Summary: Mitochondrial transfer plays a crucial role in drug resistance acquisition in multiple myeloma, with bi-directional transfer occurring through tunneling nanotubes and partial cell fusion, especially under the influence of chemotherapeutic drugs. By influencing tumor metabolism and using oxidative phosphorylation inhibitors alongside chemotherapy, the supportive effect of stromal cells can be effectively avoided in myeloma cells.
Review
Oncology
Patricia Maiso, Pedro Mogollon, Enrique M. Ocio, Mercedes Garayoa
Summary: Multiple myeloma is a cancer of plasma cells that accumulate in the bone marrow, and the interaction between mesenchymal stromal cells and myeloma cells appears to play a crucial role in the progression of the disease. Structural and functional differences between mesenchymal stromal cells from healthy donors and myeloma patients have been identified, suggesting a significant contribution to the pathophysiology of multiple myeloma.
Review
Cell Biology
Qianmin Gao, Lipeng Wang, Sicheng Wang, Biaotong Huang, Yingying Jing, Jiacan Su
Summary: Bone marrow mesenchymal stromal cells are a group of heterogeneous cells consisting of multipotent stem cells, including osteochondral and adipocyte progenitors. Recent studies have identified new subgroups of BMSCs and clarified their roles in normal and pathological conditions. Furthermore, key regulators and mechanisms controlling the fate of BMSCs have been revealed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Oncology
Maria A. Karalexi, Andreas Katsimpris, Paraskevi Panagopoulou, Panagiota Bouka, Joachim Schuz, Evangelia Ntzani, Eleni Th Petridou, Marina Servitzoglou, Margarita Baka, Maria Moschovi, Maria Kourti, Vassilios Papadakis, Sofia Polychronopoulou, Emmanouel Hatzipantelis, Maria Ioannidou, Helen Dana, Iordanis Pelagiadis, Eftichia Stiakaki, Kalliopi Stefanaki, Katerina Strantzia, Maro Gavra, Astero Malama, Andreas Mitsios, Evdoxia Bouka
Summary: This systematic review and meta-analysis examined the association between maternal modifiable lifestyle factors and neuroblastoma risk in children. The results suggest that maternal smoking and alcohol consumption are not significantly associated with neuroblastoma risk, while maternal vitamin intake during pregnancy is associated with a significantly lower risk of neuroblastoma.
CANCER EPIDEMIOLOGY
(2022)
Review
Pediatrics
Iordanis Pelagiadis, Ioannis Kyriakidis, Nikolaos Katzilakis, Chrysoula Kosmeri, Danai Veltra, Christalena Sofocleous, Stavros Glentis, Antonis Kattamis, Alexandros Makis, Eftichia Stiakaki
Summary: Diamond-Blackfan anemia (DBA) is a ribosomopathy characterized by bone marrow erythroid hypoplasia and severe anemia in infancy. In addition to classic DBA, other variations have been identified. Splice variants are common in DBA and have a significant impact on ribosome translation and levels. This study presents two cases with novel pathogenic splice variants.
