Article
Biochemistry & Molecular Biology
Huanhuan Li, Hongji Li, Shuo Chen, Wenhui Wu, Peng Sun
Summary: Terpene synthases are widely distributed in Actinobacteria, and genome sequencing of Streptomyces sp. NRRL S-4 unveiled a biosynthetic gene cluster that synthesizes pentalenolactone type terpenes. Chemical investigation of the S-4 strain led to the isolation of two new sesquiterpenoids, which showed moderate antimicrobial activities against both Gram-positive and Gram-negative bacteria. These findings confirm the functionality of the pentalenolactone pathway in this organism and pave the way for further exploration using genome mining strategies.
Article
Biochemistry & Molecular Biology
Manyu Zhang, Jinwei Ren, Yuanming Luo, Weidong Xie, Erwei Li
Summary: Six new polyene carboxylic acids named serpentemycins E-J, together with three known analogs, were isolated from the fermentation medium of Streptomyces sp. TB060207, which was isolated from arid soil collected from Tibet, China. The structures of the new compounds were elucidated based on HR-ESI-MS and NMR spectroscopic analyses. The inhibitory activities of compounds 1-9 against NO production in LPS-activated RAW264.7 cells were evaluated, and compound 9 showed an inhibition rate of 87.09% to 60.53% at concentrations ranging from 5.0 to 40.0 μM.
Article
Biotechnology & Applied Microbiology
Heung-Soon Park, Hee-Ju Nah, Seung-Hoon Kang, Si-Sun Choi, Eung-Soo Kim
Summary: A potential novel phytopathogenic fungicide-producing Streptomyces strain, S. rubrisoli Inha 501, was identified through bioassay-based screening, showing potent antifungal activities against various fungi. Whole-genome sequencing and anti-SMASH genome mining revealed a polyene biosynthetic gene cluster and confirmed the compound's homology to neotetrafibricin A, suggesting it as a promising candidate for crop protection against phytopathogenic fungi in the soil environment.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Microbiology
Khorshed Alam, Arpita Mazumder, Suranjana Sikdar, Yi-Ming Zhao, Jinfang Hao, Chaoyi Song, Yanyan Wang, Rajib Sarkar, Saiful Islam, Youming Zhang, Aiying Li
Summary: Natural products derived from Streptomyces play a crucial role in the treatment of diseases and agriculture, yet many remain unexplored. The development of novel antibiotics, agrochemicals, and anticancer medicines is imperative due to increasing resistance. Genomic-driven drug discovery strategies offer a promising future for drug development from Streptomyces.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lan-Fang Li, Qing-Xuan Wu, Hao Wu, Yao Li, Qian Peng, Ren-Hao Han, Da-Hu Zhang, Wei-Dong Yu, Rui Xu, Juan Wang, Zhaobin Fan, Shao-Yang Hou
Summary: Streptomyces sp. HP-A2021, isolated from rhizosphere soil of Ginkgobiloba L., was found to harbor a wealth of cryptic secondary metabolite biosynthetic gene clusters. Genome sequencing and bioinformatics analysis revealed the presence of 33 secondary metabolite biosynthetic gene clusters, including the putative thiotetroamide, alkylresorcinol, coelichelin, and geosmin. Antibacterial activity assays confirmed that HP-A2021 showed potent antimicrobial activity against human pathogenic bacteria.
BIOCHEMICAL GENETICS
(2023)
Article
Microbiology
Juan Hu, Zi-Xuan Wang, Pei-Meng Li, Pei-Yuan Qian, Ling-Li Liu
Summary: This study investigated the chemical composition and biosynthesis pathway of compounds produced by Streptomyces sulphureus DSM 40104. With the guild of molecular networking analysis, we isolated and identified six uncommon structural characteristics of compounds, including four newly discovered pyridinopyrones. Based on genomic analysis, we proposed a possible hybrid NRPS-PKS biosynthesis pathway for pyridinopyrones. Compounds 1-3 exhibited moderate anti-neuroinflammatory activity against LPS-induced BV-2 cell inflammation. Our study demonstrates the diversity of polyene pyrone compounds regarding their chemical structure and bioactivity while providing new insights into their biosynthesis pathway. These findings may lead to the development of new treatments for inflammation-related diseases.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Jing Shi, Yang Shi, Jian Cheng Li, Wanqing Wei, Yu Chen, Ping Cheng, Cheng Li Liu, Hao Zhang, Rui Wu, Bo Zhang, Rui Hua Jiao, Shouyun Yu, Yong Liang, Ren Xiang Tan, Hui Ming Ge
Summary: This study successfully revealed the mechanism of benzene ring formation in the biosynthesis of cinnamoyl moiety and identified two distinct enzymes that can synthesize benzene rings via polyene precursors.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Medicinal
Huaran Zhang, Yingying Chen, Yanqing Li, Yongxiang Song, Junying Ma, Jianhua Ju
Summary: The study on Streptomyces sp. SCSIO ZS0520 revealed new secondary metabolites and conducted bioinformatic analysis to lay foundations for solving remaining biosynthetic issues.
Article
Biotechnology & Applied Microbiology
Medhat Rehan, Abdellatif Gueddou, Abdulaziz Alharbi, Imen Ben Abdelmalek
Summary: In this study, a newly isolated strain of Streptomyces, named HM3, from arid and sandy soil in Qassim, SA, showed inhibitory activity against animal pathogenic bacteria and fungi. Genome analysis predicted the presence of multiple biosynthetic gene clusters for secondary metabolites, indicating the potential for discovering novel natural products.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Chung Thanh Nguyen, Adzemye Fovennso Bridget, Van Thuy Thi Pham, Hue Thi Nguyen, Tae-Su Kim, Jae Kyung Sohng
Summary: In this study, Streptomyces sp. KCTC 0041BP isolated from soil in Cheolwon, Republic of Korea, was found to have a large genome size and diverse secondary metabolite biosynthetic gene clusters and carbohydrate-active enzymes. A total of 21 compounds were successfully identified from this strain, with the structure of 8 compounds confirmed. The results demonstrate the strain's potential to produce numerous useful compounds, including bioactive compounds with antibacterial and antifungal activities.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Tao Liu, Zhen Ren, Wei-Xun Chunyu, Gui-Ding Li, Xiu Chen, Zhou-Tian-Le Zhang, Hui-Bing Sun, Mei Wang, Tian-Peng Xie, Meng Wang, Jing-Yuan Chen, Hao Zhou, Zhong-Tao Ding, Min Yin
Summary: The talented endophytic bacteria strain YINM00001 isolated from a Chinese medicinal herb exhibited strong antimicrobial activity and multiple antibiotic resistances. Genomic analysis revealed the potential for producing valuable secondary metabolites, making it an ideal candidate for further biosynthetic studies and production improvement. Further exploration is needed to uncover more novel compounds from this unique Streptomyces strain.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Applied
Yi Zhao, Hu Chen, Lu Yang, Wenli Li, Huayue Li
Summary: A new polyene aldehyde, named amphonal (1), and two known polyketides were isolated from the deep-sea-derived Streptomyces amphotericinicus OUCT16-38 strain. Compound 2 showed significant growth inhibition against drug-resistant human lung cancer cells and exhibited cytotoxic effects towards other lung cancer cell lines as well.
NATURAL PRODUCT RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Constanze Paulus, Oleksandr Gromyko, Andriy Luzhetskyy
Summary: During the screening of new streptomycete strains, the strain Streptomyces sp. Cl 58-27 was found to produce a novel ansamycin natural product, kendomycin E, structurally similar to kendomycins. The structure of kendomycin E was elucidated using NMR spectroscopy, and its biosynthetic gene cluster was identified through genome sequencing and bioinformatic analysis of Streptomyces sp. Cl 58-27.
Article
Biotechnology & Applied Microbiology
Ziheng An, Hui Tao, Yong Wang, Bingqing Xia, Yang Zou, Shuai Fu, Fang Fang, Xiao Sun, Renqiong Huang, Yao Xia, Zixin Deng, Ran Liu, Tiangang Liu
Summary: This study utilized genetic engineering and fermentation optimization to improve the production of spinosad in heterologous Streptomyces strains. Overexpression of polyketide synthase genes and increased supply of precursor molecules were found to enhance spinosad production, resulting in the highest titer achieved in heterologous Streptomyces species. These findings provide valuable strategies for the efficient heterologous production of natural products.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2021)
Article
Microbiology
Yoon-Hee Chung, Hiyoung Kim, Chang-Hun Ji, Hyun-Woo Je, Dongho Lee, Sang Hee Shim, Hwang-Soo Joo, Hahk-Soo Kang
Summary: The genus Streptomyces is a rich source of secondary metabolite biosynthetic gene clusters (BGCs), with many cryptic BGCs still undiscovered. Through comparative genomics, a specific phylogenetic lineage within Streptomyces with high and uncharacterized biosynthetic potential was identified. Members of this lineage carry numerous unique BGCs, making them a promising target for metabolite characterization studies.