Review
Cell & Tissue Engineering
Wangwang Luo, Yang Wang, Qing Han, Zhonghan Wang, Jianhang Jiao, Xuqiang Gong, Yang Liu, Aobo Zhang, Han Zhang, Hao Chen, Jincheng Wang, Minfei Wu
Summary: This review discusses recent advances in enthesis tissue engineering, particularly the use of biological scaffolds, and provides perspectives on future directions in this field.
JOURNAL OF TISSUE ENGINEERING
(2022)
Review
Biochemistry & Molecular Biology
Shabnam Anjum, Ting Li, Mohammad Saeed, Qiang Ao
Summary: Tissue engineering has emerged as a primary research topic for treating diseased or damaged tendon/ligament tissue, and decellularized extracellular matrix (dECM) scaffolds show promise for restoring tissue function.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Cell & Tissue Engineering
David L. Butler
Summary: This paper reviews the history of research leading up to the introduction of functional tissue engineering (FTE) for tendon and ligament repair, highlighting the importance of collaborations among surgeons, biologists, and engineers.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2022)
Article
Biotechnology & Applied Microbiology
Alexander R. A. Szojka, David Xinzheyang Li, Malou E. J. Sopcak, Zhiyao Ma, Melanie Kunze, Aillette Mulet-Sierra, Samer M. Adeeb, Lindsey Westover, Nadr M. Jomha, Adetola B. Adesida
Summary: Experimental conditions combining mechanical loading and hypoxia can enhance the development of human meniscus fibrocartilage. Properly applied mechano-hypoxia conditioning may support the development of non-hypertrophic articular cartilage.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Engineering, Biomedical
Chiara Rinoldi, Ewa Kijenska-Gawronska, Ali Khademhosseini, Ali Tamayol, Wojciech Swieszkowski
Summary: Injuries to tendons and ligaments are common and have a significant impact on daily life. Current treatment strategies are sometimes inadequate, leading to the development of tissue engineering and regenerative medicine approaches aiming to stimulate regeneration. The design of artificial scaffolds with tailored mechanical properties is crucial for restoring functionality in such cases.
ADVANCED HEALTHCARE MATERIALS
(2021)
Review
Materials Science, Biomaterials
Yunkai Tang, Zhen Wang, Lei Xiang, Zhenyu Zhao, Wenguo Cui
Summary: This article summarizes the latest progress in cells, biomaterials, active molecules and construction technology in treating tendon/ligament injuries. The characteristics of supports made of different materials and the development and application of different manufacturing methods are discussed. The development of electrospinning and hydrogel technology has diversified the production and treatment of materials.
REGENERATIVE BIOMATERIALS
(2022)
Article
Engineering, Biomedical
Nehar Celikkin, Dario Presutti, Fabio Maiullari, Marina Volpi, Yurii Promovych, Konrad Gizynski, Joanna Dolinska, Agnieszka Wisniewska, Marcin Opallo, Alessia Paradiso, Chiara Rinoldi, Claudia Fuoco, Wojciech Swieszkowski, Claudia Bearzi, Roberto Rizzi, Cesare Gargioli, Marco Costantini
Summary: This paper introduces an innovative high-throughput rotary wet-spinning biofabrication method for manufacturing cellularized constructs composed of highly-aligned hydrogel fibers. The proposed system utilizes a microfluidic printing head with a crosslinking bath microtank and a co-axial nozzle for immediate gelation of the extruded fibers. The system is suitable for in vitro engineering of functional myo-substitutes.
Article
Chemistry, Multidisciplinary
Frederik Schlottmann, Sarah Strauss, Christian Plaass, Bastian Welke, Peter M. Vogt, Joern W. Kuhbier
Summary: The study focused on using spider silk as a carrier matrix to construct tendon-like tissue with high cell vitality and morphology comparable to tenocytes. Although promising, further research is needed to validate its potential in patient-specific tendon reconstruction.
APPLIED SCIENCES-BASEL
(2021)
Article
Chemistry, Multidisciplinary
Zhirui Dong, Ruoxuan Peng, Yuehua Zhang, Yicheng Shan, Wang Ding, Yifan Liu, Jian Li, Mingdong Zhao, Li-Bo Jiang, Shengjie Ling
Summary: This study utilized a bio-inspired fibrous artificial tendon (FAT) to repair Achilles tendon defects. The FAT exhibited a highly ordered molecular and nanofibrillar architecture similar to tendons, and promoted the expression of tendon-related genes. These findings suggest the potential clinical applications of FAT.
Review
Chemistry, Multidisciplinary
Darshan Tagadur Govindaraju, Chih-Hao Chen, K. T. Shalumon, Hao-Hsi Kao, Jyh-Ping Chen
Summary: The limited natural healing capacity of tendons and ligaments presents a challenge for effective therapeutic strategies. Tissue engineering, using biomaterials, cells, and biochemical signals, has shown promising results in restoring the physiological functions of these tissues. This paper reviews the structure and healing mechanisms of tendons and ligaments, and discusses the use of bioactive nanostructured scaffolds, with a focus on electrospun fibrous scaffolds, in tissue engineering.
Review
Engineering, Biomedical
Yexin Li, Ting Deng, Dilihumaer Aili, Yang Chen, Weihong Zhu, Qian Liu
Summary: This review summarizes the application of cell sheet technology in the treatment of tendon and ligament injuries, focusing on the effects of stem cell-derived sheets in mitigating inflammation and promoting healing. It also discusses the prerequisites for future clinical translation.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Keisuke Fukada, Koji Tachibana, Yuta Kurashina, Yosuke Kaneko, Tatsuaki Matsumoto, Takeshi Miyamoto, Yasuo Niki, Masaya Nakamura, Hiroaki Onoe
Summary: This paper presents a scalable microfiber-shaped tissue for macroscale tendon tissue reconstruction in vitro. The microfiber-shaped tendon-like tissues maintained their morphology and showed proper expression of tenomodulin and actin filaments. A macro-scale tendon tissue assembly was successfully constructed by bundling the microfiber-shaped tendon-like tissues, allowing for uniform cell distribution.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Review
Engineering, Biomedical
Tingyun Lei, Tao Zhang, Wei Ju, Xiao Chen, Boon Chin Heng, Weiliang Shen, Zi Yin
Summary: This review discusses the challenges and strategies for tendon/ligament-to-bone healing, including biomimetic materials, growth factors, and stem cells. The importance of structure-function relationship, growth factor concentration, and stem cell application in the healing process is highlighted. Unmet needs and emerging strategies in tendon/ligament-to-bone regeneration are also discussed.
BIOACTIVE MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Chih-Hao Chen, Dai-Ling Li, Andy Deng-Chi Chuang, Banendu Sunder Dash, Jyh-Ping Chen
Summary: The study prepared core-sheath nanofibers with different structures for tendon tissue engineering, and demonstrated that the combined effects of aligned fibers and PRP could enhance cell proliferation rates and gene expression in tenocytes. Additionally, dynamic cell culture in a bioreactor with cyclic tension stimulation could accelerate tendon maturation in vitro, leading to improved cell proliferation rates and maintenance of tenogenic phenotype compared to static culture.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Materials Science, Multidisciplinary
Riccardo Gottardi, Kim Moeller, Roberto Di Gesu, Rocky S. Tuan, Martijn van Griensven, Elizabeth R. Balmayor
Summary: The study utilized hyperelastic biphasic 3D printed PLGA scaffolds to mimic the native insertion side, and cultured constructs in dual fluidic bioreactors to achieve regional differentiation towards tenogenic or chondrogenic fate. This system has been validated as a potential platform to form the tendon enthesis and will be further optimized for the fabrication of distinctly biphasic constructs in future studies.
FRONTIERS IN MATERIALS
(2021)