Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 99, Issue 22, Pages 9591-9604Publisher
SPRINGER
DOI: 10.1007/s00253-015-6772-1
Keywords
Trichoderma harzianum; Second-generation ethanol; Cellulase; Endoglucanase; Aspergillus niger
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Funding
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2008/56255-9, 2009/52840-7, 2009/05328-9, 2010/18773-8, 2011/20977-3, 2011/05712-3]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [490022/2009-0, 301981/2011-6, 400045/2012-5]
- CAPES
- Universidade de Sao Paulo via grant Centro de Instrumentacao para estudos avancados de materiais nanoestruturados e biossistemas
- Universidade de Sao Paulo via grant Nucleo de Apoio a Pesquisa em Bioenergia e Sustentabilidade (NAPBS)
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Trichoderma filamentous fungi have been investigated due to their ability to secrete cellulases which find various biotechnological applications such as biomass hydrolysis and cellulosic ethanol production. Previous studies demonstrated that Trichoderma harzianum IOC-3844 has a high degree of cellulolytic activity and potential for biomass hydrolysis. However, enzymatic, biochemical, and structural studies of cellulases from T. harzianum are scarce. This work reports biochemical characterization of the recombinant endoglucanase I from T. harzianum, ThCel7B, and its catalytic core domain. The constructs display optimum activity at 55 A degrees C and a surprisingly acidic pH optimum of 3.0. The full-length enzyme is able to hydrolyze a variety of substrates, with high specific activity: 75 U/mg for beta-glucan, 46 U/mg toward xyloglucan, 39 U/mg for lichenan, 26 U/mg for carboxymethyl cellulose, 18 U/mg for 4-nitrophenyl beta-d-cellobioside, 16 U/mg for rye arabinoxylan, and 12 U/mg toward xylan. The enzyme also hydrolyzed filter paper, phosphoric acid swollen cellulose, Sigmacell 20, Avicel PH-101, and cellulose, albeit with lower efficiency. The ThCel7B catalytic domain displays similar substrate diversity. Fluorescence-based thermal shift assays showed that thermal stability is highest at pH 5.0. We determined kinetic parameters and analyzed a pattern of oligosaccharide substrates hydrolysis, revealing cellobiose as a final product of C6 degradation. Finally, we visualized effects of ThCel7B on oat spelt using scanning electron microscopy, demonstrating the morphological changes of the substrate during the hydrolysis. The acidic behavior of ThCel7B and its considerable thermostability hold a promise of its industrial applications and other biotechnological uses under extremely acidic conditions.
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