Article
Pharmacology & Pharmacy
Susi Burgalassi, Erica Zucchetti, Leonardo Ling, Patrizia Chetoni, Silvia Tampucci, Daniela Monti
Summary: This paper focuses on the preparation and characterization of hydrogels made from different blends of polyvinyl alcohol (PVA) with microcrystalline cellulose (MCC) and gelatin (GEL), as well as gelatin and chitosan alone, for use as corneal stroma substitutes in drug permeability tests. The results show that the PVA/MCC blend and chitosan have promising mechanical and permeability properties similar to the corneal stroma.
Review
Medicine, Research & Experimental
Sougat Das, Debasmita Saha, Saptarshi Majumdar, Lopamudra Giri
Summary: This article discusses the formulation and evaluation of hydrogels in the ocular drug delivery system for glaucoma treatment, providing insights into addressing the challenges associated with current anti-glaucoma drug formulations.
MOLECULAR PHARMACEUTICS
(2022)
Article
Toxicology
Valentina Citi, Fabio Neggiani, Marco Sanso, Vincenzo Calderone
Summary: In vitro reconstructed human corneal tissue models were used to evaluate the potential therapeutic effects of a novel ophthalmic formulation on eye surface symptoms. The experimental results showed that the formulation protected the tissues from damage and mildly reduced the anti-inflammatory mediator TNF-alpha.
TOXICOLOGY IN VITRO
(2021)
Article
Biochemistry & Molecular Biology
Manuel Chacon, Manuel Sanchez, Natalia Vazquez, Mairobi Persinal-Medina, Sergio Alonso-Alonso, Begona Baamonde, Jose F. Alfonso, Luis Fernandez-Vega-Cueto, Jesus Merayo-Lloves, Alvaro Meena
Summary: Impedance analysis can be used to evaluate cell maturation and detect slight cell damages in RhCE models, providing a faster and simpler method for assessing ocular irritancy with comparable accuracy to current methodologies.
BIOELECTROCHEMISTRY
(2022)
Article
Chemistry, Medicinal
Anna Matysik-Wozniak, Waldemar A. Turski, Monika Turska, Roman Paduch, Miroslaw Lancut, Pawel Piwowarczyk, Miroslaw Czuczwar, Robert Rejdak
Summary: The study evaluated the effects of KYNA on corneal and conjunctival cells in vitro and the re-epithelization of corneal erosion in rabbits in vivo. KYNA showed potential in promoting corneal epithelial cell migration, reducing inflammatory cytokine secretion, and accelerating corneal epithelium healing.
Article
Pharmacology & Pharmacy
Sadek Ahmed, Maha M. Amin, Sarah Mohamed El-Korany, Sinar Sayed
Summary: The purpose of this study was to develop and optimize Fenticonazole Nitrate-loaded novasomes for enhanced corneal penetration and antifungal activity. The optimized formula showed high entrapment efficiency, improved corneal permeation, and increased antifungal activity. The results confirmed the validity of novasomes in improving the corneal penetration and antifungal activity of Fenticonazole Nitrate.
Article
Multidisciplinary Sciences
Tsutomu Imaizumi, Ryuhei Hayashi, Yuji Kudo, Xiaoqin Li, Kaito Yamaguchi, Shun Shibata, Toru Okubo, Tsuyoshi Ishii, Yoichi Honma, Kohji Nishida
Summary: The conditioned medium of human adipose-derived mesenchymal stem cells (hAdMSC-CM) has been found to suppress cytotoxicity and inflammation in human corneal epithelial cells (hCECs), improve barrier dysfunction, and serve as a potential therapeutic agent for dry eye syndrome (DES).
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Munetoyo Toda, Morio Ueno, Jun Yamada, Asako Hiraga, Kazuko Asada, Junji Hamuro, Chie Sotozono, Shigeru Kinoshita
Summary: This study found that injection of cultured human corneal endothelial cell suspension into the anterior chamber can induce immune tolerance that contributes to the survival of reconstituted endothelium, and no signs of immune rejection were observed during the 5-8 years of postoperative follow-up.
SCIENTIFIC REPORTS
(2022)
Article
Ophthalmology
Ghasem Yazdanpanah, Ritu Shah, Sri Raghurama R. Somala, Khandaker N. Anwar, Xiang Shen, Seungwon An, Meisam Omidi, Mark Rosenblatt, Tolou Shokuhfar, Ali R. Djalilian
Summary: COMatrix hydrogel demonstrated enhanced attachment and proliferation of human corneal epithelial cells in vitro, as well as accelerated corneal epithelial wound closure in a murine model in vivo. The hydrogel also showed reduced expression of inflammatory genes and increased expression of a proliferation marker in wounded corneal epithelium. These findings suggest the potential therapeutic application of COMatrix hydrogel as an ocular surface bandage for enhancing epithelial wound healing.
Article
Chemistry, Medicinal
Fouzia Gherrak, Abdelkader Hadjsadok, Sonia Lefnaoui
Summary: In this study, oral granules that easily dissolved in water were prepared with a formulation of sodium alginate, chitosan, and sodium carboxymethylcellulose. The gels showed a firm and homogeneous structure when subjected to simulated gastric fluid, and had potential applications as anti-obesity medications. The interaction between alginate and chitosan was confirmed by Fourier transform infrared analysis, and the gels exhibited varied elastic storage modulus values and liquid absorption capabilities depending on the concentrations of the three biopolymers used.
DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
(2021)
Article
Ophthalmology
Zhenying Shang, Chenxi Li, Xuemei Liu, Manhong Xu, Xiaomin Zhang, Xiaorong Li, Colin J. Barnstable, Shaozhen Zhao, Joyce Tombran-Tink
Summary: The study reveals that the lack of PEDF gene results in reduced corneal innervation and sensitivity, increased corneal surface injury, and altered tear production. The expression of PEDF in the cornea and supporting tissues plays a crucial role in maintaining corneal function.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Polymer Science
Zeinab Fathalla, Adel Al Fatease, Hamdy Abdelkader
Summary: Innovative hybrid chitosan-sodium alginate microparticles were fabricated using two different techniques and studied for various properties. They showed controlled drug release, effective mucoadhesive properties, and improved corneal permeation compared to the control solution. Furthermore, the microparticles exhibited no cytotoxicity and were well tolerated by human lens and corneal epithelial cell lines.
Article
Cell Biology
Santhosh Kacham, Tejal Sunil Bhure, Sindhuja D. Eswaramoorthy, Gaurav Naik, Subha Narayan Rath, Sreenivasa Rao Parcha, Sayan Basu, Virender Singh Sangwan, Sachin Shukla
Summary: Umbilical cord-derived mesenchymal stem cells promote the repair of injured corneal epithelium by stimulating the proliferation of corneal epithelial cells, potentially serving as a non-ocular source of stem cells for treating injury-induced bilateral corneal diseases.
Article
Ophthalmology
Shu-I Yeh, Sung-Hsun Yu, Hsiao-Sang Chu, Chin-Te Huang, Yeou-Ping Tsao, Chao-Min Cheng, Wei-Li Chen
Summary: The study investigated the potential of PEDF peptide 44-mer in promoting nerve regeneration in a rabbit corneal nerve injury model and its neurotrophic ability in cultivated mouse trigeminal neuron cells. The results demonstrated that 44-mer could promote corneal nerve regeneration in vivo and had neurotrophic effects in vitro.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Medicine, Research & Experimental
Ioannis I. Andreadis, Christina Karavasili, Angelos Thomas, Anastasia Komnenou, Manolis Tzimtzimis, Dimitrios Tzetzis, Dimitrios Andreadis, Nikolaos Bouropoulos, Dimitrios G. Fatouros
Summary: Intraocular pressure reduction regimens for glaucoma often involve eye drops, which have limitations such as short residence time and reduced patient compliance. In situ gelling nanofiber films were developed for ocular delivery of timolol maleate, showing sustained IOP-lowering effects and improved drug release compared to conventional eye drops.
MOLECULAR PHARMACEUTICS
(2022)
Article
Chemistry, Physical
Giuseppe Angellotti, Denise Murgia, Alessandro Presentato, Maria Cristina D'Oca, Amalia Giulia Scarpaci, Rosa Alduina, Maria Valeria Raimondi, Viviana De Caro
Article
Infectious Diseases
Maria Valeria Raimondi, Alessandro Presentato, Giovanna Li Petri, Miriam Buttacavoli, Agnese Ribaudo, Viviana De Caro, Rosa Alduina, Patrizia Cancemi
Article
Biochemistry & Molecular Biology
Denise Murgia, Giuseppe Angellotti, Alice Conigliaro, Francesco Carfi Pavia, Fabio D'Agostino, Marco Contardi, Rodolfo Mauceri, Riccardo Alessandro, Giuseppina Campisi, Viviana De Caro
Review
Biochemistry & Molecular Biology
Giuseppe Angellotti, Denise Murgia, Giuseppina Campisi, Viviana De Caro
Article
Pharmacology & Pharmacy
Viviana De Caro, Libero Italo Giannola, Giulia Di Prima
Summary: By utilizing solid lipid microparticles (SLMs) as a carrier technology, the limitations of antifungal therapy for oral candidiasis (OC) can be effectively overcome, enhancing drug accumulation in tissues. SLMs exhibit excellent drug loading properties, allowing for reduced drug doses and improved therapeutic effects.
Article
Pharmacology & Pharmacy
Giulia Di Prima, Giuseppe Angellotti, Amalia Giulia Scarpaci, Denise Murgia, Fabio D'agostino, Giuseppina Campisi, Viviana De Caro
Summary: The study evaluated the transmucosal behavior of resveratrol through the porcine sublingual mucosa and prepared fast-disintegrating sublingual mini-tablets using chemical permeation enhancers.
Article
Pharmacology & Pharmacy
Giuseppe Angellotti, Alessandro Presentato, Denise Murgia, Giulia Di Prima, Fabio D'Agostino, Amalia Giulia Scarpaci, Maria Cristina D'Oca, Rosa Alduina, Giuseppina Campisi, Viviana De Caro
Summary: Immediate implant placement is a restorative approach widely used to address ridge remodeling after tooth extraction. The success of this procedure depends on timely osseointegration in surrounding tissues. A multifunctional nanocomposite designed for this purpose has shown promising properties in supporting single-stage dental restorative treatment.
Article
Pharmacology & Pharmacy
Giuseppe Angellotti, Giulia Di Prima, Amalia Giulia Scarpaci, Fabio D'Agostino, Giuseppina Campisi, Viviana De Caro
Summary: Cytisine (CYT) is proposed for buccal administration to overcome its unfavorable pharmacokinetic properties. The spray-drying method is used to obtain uniform CYT-loaded pharmaceutical powders, which are compressed to create buccal delivery systems (BDSs). The BDSs enhance CYT permeation and have the potential to achieve therapeutic effects based on in vitro and mathematical evaluations.
Article
Chemistry, Multidisciplinary
Giulia Di Prima, Antonino Scurria, Giuseppe Angellotti, Elena Belfiore, Mario Pagliaro, Francesco Meneguzzo, Viviana De Caro, Rosaria Ciriminna
Summary: The comparison between grapefruit IntegroPectin powders isolated via spray drying and freeze drying provides relevant information regarding phenolic content, quantification of flavonoids, radical scavenging activity, total protein content, and pH of aqueous solutions. The two drying methods yield similar pectins, except for protein content and antioxidant power. Optimizing the spray drying parameters allows for a nearly quantitative yield, approaching that obtained via freeze drying (>95%).
SUSTAINABLE CHEMISTRY AND PHARMACY
(2022)
Review
Pharmacology & Pharmacy
Giuseppe Angellotti, Giulia Di Prima, Elena Belfiore, Giuseppina Campisi, Viviana De Caro
Summary: Oral squamous cell carcinoma (OSCC) is a prevalent and aggressive cancer, and conventional treatments have limitations. Resveratrol (RSV), a natural compound, has shown potential for treating OSCC due to its antiproliferative, antimetastatic, and proapoptotic effects. RSV also synergizes with chemotherapeutics and overcomes drug resistance. However, there is a lack of specific investigations on RSV's effects in different administration routes and vehicles. RSV-loaded liposomes have demonstrated efficacy against OSCC models, suggesting the development of drug delivery systems as a turning point in oral cancer treatment.
Article
Agriculture, Dairy & Animal Science
Cristina Giosue, Fabio D'Agostino, Giuseppe Maniaci, Giuseppe Avellone, Marzia Sciortino, Viviana De Caro, Adriana Bonanno, Marialetizia Ponte, Marco Alabiso, Antonino Di Grigoli
Summary: The study evaluated the risk and benefits of consuming typical stretched cheeses, focusing on their fatty acid profile and persistent organic pollutants content. Cheeses made in spring showed a healthier fatty acid profile, while those made in winter contained higher levels of contaminants. High levels of saturated fatty acids and environmental pollutants were found in cheeses from non-organic farms, posing a potential health risk to consumers.
Article
Biochemistry & Molecular Biology
Giulia Di Prima, Elena Belfiore, Martina Migliore, Amalia Giulia Scarpaci, Giuseppe Angellotti, Ignazio Restivo, Mario Allegra, Vincenzo Arizza, Viviana De Caro
Summary: This study validated the potential of waste bentonite from the grape processing industries as a valuable matrix for polyphenol extraction. By applying a waste-to-market approach, functional excipients were produced. The best extract was obtained from wet bentonite with PEG200, showing the highest phenolic content and strongest antioxidant activity. It can be directly used as a polyphenol-enriched functional raw material for cosmetics and pharmaceuticals.
Article
Biochemistry & Molecular Biology
Viviana De Caro, Giuseppe Angellotti, Fabio D'Agostino, Giulia Di Prima
Summary: This study aimed to design CYT-loaded buccal thin films that can promote the interaction between CYT and buccal mucosa for transbuccal drug delivery. Several compositions were prepared using the solvent casting method, and four of them showed promising aspects and flexibility. These films demonstrated homogeneity, thinness, low swelling degree, and controlled drug release, and exhibited mucoadhesive effects and potent permeation enhancement when tested on ex vivo porcine buccal mucosa.
Article
Biochemistry & Molecular Biology
Giuseppe Angellotti, Giulia Di Prima, Fabio D'Agostino, Emanuela Peri, Maria Rita Tricoli, Elena Belfiore, Mario Allegra, Patrizia Cancemi, Viviana De Caro
Summary: In this study, a complex mixture was designed to encapsulate Resveratrol (RSV) and produce lipid nanoparticles (LNPs) for enhanced wound healing. The optimized lipid mixture effectively encapsulated RSV and exhibited suitable nanosize and antioxidant properties. The release studies demonstrated the ability of the LNPs to release RSV under wound conditions. Biological and microbiological evaluations showed that the LNPs promoted fibroblast proliferation and migration, and exhibited antibiofilm properties at low doses. Therefore, the multicomponent LNPs could serve as a valuable RSV delivery platform for wound healing purposes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Rodolfo Mauceri, Denise Murgia, Orazio Cicero, Luigi Paterno, Luca Fiorillo, Viviana De Caro, Giuseppina Campisi
Summary: The study investigates the effectiveness of using L-PRF and L-PRF blocks in treating critical-size alveolar bone defects, showing positive outcomes in achieving bone healing and tissue regeneration in selected cases.
MEDICINA-LITHUANIA
(2021)