Review
Pharmacology & Pharmacy
Kai-Di Ni, Jun-Yan Liu
Summary: CYP omega-hydroxylases are a subfamily of enzymes that mediate the metabolism of fatty acids and their metabolites. The metabolites have pleiotropic effects in inflammation and related diseases. Further studies are needed to understand the function and mechanisms of other CYP omega-hydroxylases-mediated eicosanoids in order to develop therapeutic strategies for various diseases.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Martyna Fronczek, Joanna Katarzyna Strzelczyk, Krzysztof Biernacki, Silvia Salatino, Tadeusz Osadnik, Zofia Ostrowska
Summary: The study investigated the role of genetic variation in the CYP2R1 gene in vitamin D deficiency among healthy Polish individuals. Two substitutions were found in selected gene fragments, with one potentially influencing the structure and function of CYP2R1. Further functional verification is needed to confirm these findings.
Review
Biochemistry & Molecular Biology
Maria Norlin, Kjell Wikvall
Summary: Vitamin D signaling plays a crucial role in maintaining calcium balance for bone health and has various functions in different tissues. Disturbances in vitamin D signaling are associated with numerous diseases. This review focuses on the identification of bioactivating enzymes and their genes involved in the production of active vitamin D metabolites. It evaluates species- and tissue-specific expression, enzyme kinetics, substrate specificity, and the consequences of gene mutations. The roles of different vitamin D receptors and alternative bioactivation pathways are also discussed. Although significant progress has been made in understanding vitamin D bioactivation enzymes, further research is needed to fully comprehend the pleiotropic effects and the mechanisms of enzymatic activation in vitamin D-induced responses.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Biotechnology & Applied Microbiology
Jingyan Zhang, Ying Sun, Yeji Wang, Xin Chen, Lu Xue, Jingjing Zhang, Xiangcheng Zhu, Yanwen Duan, Xiaohui Yan
Summary: Rubiginones are part of the angucycline family and have been shown to enhance the cytotoxicity of vincristine against drug-resistant cancer cell lines. Through genome mining of Streptomyces sp. CB02414, new rubiginones were discovered and a biosynthetic pathway was proposed based on the production profiles of wild-type and mutant strains. This research paves the way for further studies on cytochrome P450-catalyzed oxidation of angucyclines and the development of novel compounds through combinatorial biosynthesis strategies.
MICROBIAL CELL FACTORIES
(2021)
Review
Agriculture, Dairy & Animal Science
M. J. Kuhn, V Mavangira, L. M. Sordillo
Summary: Dairy cattle are at a high risk of disease around calving due to compromised immunity and oxidative stress, influenced by the metabolism of fatty acids and fat-soluble vitamins. The importance of the cytochrome P450 (CYP450) enzyme family in cattle health is underappreciated, with potential interactions between fatty acids and fat-soluble vitamins needing further exploration. Understanding the role of CYP450 in immune function and oxidant status could help in reducing postpartum disease occurrence.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Plant Sciences
Pramesti Istiandari, Shuhei Yasumoto, Hikaru Seki, Ery Odette Fukushima, Toshiya Muranaka
Summary: This study reveals that CPR classes I and II play different roles in triterpenoid biosynthesis in L. japonicus, with CPR class I potentially playing a crucial role in plant basal metabolism.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Ling Wang, Xiaowei Wu, Chenghua Gao, Lingrui Wei, Qian Li, Aitao Li
Summary: A new cytochrome P450 enzyme, CYP68J5_fg, was identified from Fusarium graminearum, showing steroidal 12 beta- and 15 alpha-hydroxylase activities. This is the first fungal P450 enzyme with 12 beta-hydroxylase activity discovered, offering opportunities for enhancing efficiency and selectivity in steroid drug synthesis.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Genetics & Heredity
Shuai Zhang, Song Gao, Yu Chen, Sha Xu, Shiqin Yu, Jingwen Zhou
Summary: The leaves of Ampelopsis grossedentata are rich in dihydromyricetin, which has many potential applications. In this study, hydroxylase genes involved in the biosynthesis of dihydromyricetin were identified and characterized. The hydroxylation process and carbon flux pathways of dihydromyricetin in Ampelopsis grossedentata were successfully identified.
Article
Chemistry, Medicinal
Madoka Kawamura, Yohei Kobashi, Hiroaki Tanaka, Ayako Bohno-Mikami, Makoto Hamada, Yuji Ito, Takashi Hirata, Hiroki Ohara, Naoki Kojima, Hiroko Koretsune, Emi Gunji, Takuya Fukunaga, Shoko Inatani, Yoshitaka Hasegawa, Akinori Suzuki, Teisuke Takahashi, Hiroyuki Kakinuma
Summary: 20-HETE is an important metabolite involved in the pathogenesis of renal diseases, and inhibiting its production by targeting CYP4A11 and CYP4F2 may provide a therapeutic strategy. Compound 11c, an acetylpiperidine derivative, has been identified as a potent inhibitor of 20-HETE production with favorable pharmacokinetic properties.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Nutrition & Dietetics
Vanessa Rosa Retamoso, Fernanda Barbisan, Graziele Meira Moro, Patricia Maurer, Debora Vasquez Rubio, Lauren Flores Viera dos Santos, Lyana Berro Feijoo, Matias Nunes Frizzo, Ivana Beatrice Manica da Cruz, Vanusa Manfredini, Ana Leticia Vargas Barcelos, Jacqueline da Costa Escobar Piccoli
Summary: This study evaluates the effect of the BsmI single nucleotide polymorphism (SNP) of the VDR gene on the expression of VDR, SOD2, and CYP24A1 genes in individuals with low serum vitamin D levels. The results show that the BsmI SNP of the VDR gene leads to overexpression of the CYP24A1 gene and low expression of the SOD2 gene. Therefore, the BsmI SNP of the VDR gene can modulate gene expression without interfering with serum vitamin D levels.
Article
Pharmacology & Pharmacy
Gina Mejia-Abril, Pablo Zubiaur, Marcos Navares-Gomez, Gonzalo Villapalos-Garcia, Manuel Roman, Dolores Ochoa, Francisco Abad-Santos
Summary: The study found that sex, race, and certain genetic polymorphisms can influence the pharmacokinetics and safety of dexketoprofen. However, there is currently no clear pharmacogenetic predictor that justifies individualization of therapy based on genotyping for dexketoprofen. Further research is needed to confirm the role of specific genetic polymorphisms in the pharmacokinetic variability of dexketoprofen.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Plant Sciences
Takuya Yamaguchi, Yumi Matsui, Naoki Kitaoka, Yasumasa Kuwahara, Yasuhisa Asano, Hideyuki Matsuura, Yukari Sunohara, Hiroshi Matsumoto
Summary: Nitro groups are commonly found in synthetically manufactured compounds, but loquat emits (2-nitroethyl)benzene as a flower scent. Two cytochrome P450s, CYP79D80 and CYP94A90, are identified to likely catalyze the biosynthesis of (2-nitroethyl)benzene in loquat. CYP94A90 and other CYP94As are promiscuous fatty acid omega-hydroxylases that catalyze the formation of nitro groups from aldoximes in dicot plants.
Article
Biochemistry & Molecular Biology
Taisei Yamamoto, Yoshie Hasegawa, Hiroaki Iwaki
Summary: Cytochrome P450 monooxygenases are important in metabolism. In this study, a novel self-sufficient cytochrome P450, P450(CHC), was identified and characterized in Paraburkholderia terrae KU-64, which degrades cyclohexanecarboxylate.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Melanie Nolden, Robert Velten, Mark J. I. Paine, Ralf Nauen
Summary: Resistance to common pyrethroids, such as deltamethrin and permethrin, is widespread in the malaria mosquito Anopheles funestus due to upregulated cytochrome P450 monooxygenases. The duplicated genes CYP6P9a and CYP6P9b are highly upregulated in the pyrethroid resistant strain and have been shown to metabolize various pyrethroids. Although CYP6P9 variants interact with transfluthrin, their metabolic capacity to degrade transfluthrin is low, explaining the lack of resistance observed. Therefore, transfluthrin is an important resistance-breaking pyrethroid in controlling An. funestus.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Paola Panicco, Silvia Castrignano, Sheila J. Sadeghi, Giovanna Di Nardo, Gianfranco Gilardi
Summary: Polymorphism in drug metabolism, particularly in CYP2C9 variants, affects individual response to drug dosage. This study engineered human CYP2C9 and its variants linked to a flavodoxin gene, investigating their redox properties and catalytic activities towards a marker substrate. The differences in catalytic activities among CYP2C9 variants were observed, providing a basis for measuring different catalytic responses towards drugs in therapy.
BIOELECTROCHEMISTRY
(2021)
Article
Genetics & Heredity
Wei Song, Sabrina A. Gardner, Hayk Hovhannisyan, Amanda Natalizio, Katelyn S. Weymouth, Wenjie Chen, Ildiko Thibodeau, Ekaterina Bogdanova, Stanley Letovsky, Alecia Willis, Narasimhan Nagan
GENETICS IN MEDICINE
(2016)
Article
Genetics & Heredity
Theresa Swift-Scanlan, Christopher T. Smith, Sabrina A. Bardowell, Charlotte A. Boettiger
BMC MEDICAL GENOMICS
(2014)
Article
Oncology
Sabrina A. Bardowell, Joel Parker, Cheng Fan, Jamie Crandell, Charles M. Perou, Theresa Swift-Scanlan
BREAST CANCER RESEARCH AND TREATMENT
(2013)
Article
Biochemistry & Molecular Biology
Sabrina A. Bardowell, Faping Duan, Danny Manor, Joy E. Swanson, Robert S. Parker
JOURNAL OF BIOLOGICAL CHEMISTRY
(2012)
Article
Biochemistry & Molecular Biology
Sabrina A. Bardowell, Xinxin Ding, Robert S. Parker
JOURNAL OF LIPID RESEARCH
(2012)
Article
Engineering, Environmental
Weihua Song, Sabrina Bardowell, Kevin E. O'Shea
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2007)