Article
Multidisciplinary Sciences
Kazuya Morita, Naoyuki Matsumoto, Kengo Saito, Toshihide Hamabe-Horiike, Keishi Mizuguchi, Yohei Shinmyo, Hiroshi Kawasaki
Summary: The study revealed that AQP4 is selectively expressed in astrocytes in the mouse cerebral cortex during development, and that BMP signaling plays a role in altering AQP4 levels in the cortex.
SCIENTIFIC REPORTS
(2021)
Article
Neurosciences
Sahar Sweetat, Natania Casden, Oded Behar
Summary: Injury to the central nervous system leads to neuronal cell death and astrogliosis. The lack of Sema4B expression in glial cells improves neuronal survival by reducing toxic factors or increasing survival factors secreted by glial cells.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Sabrina Amalfi, Maria del Pilar Plastine, Maria Gabriela Lopez, Maria Jose Gravisaco, Oscar Taboga, Victoria Alfonso
Summary: In this study, researchers demonstrated that the poxin homologous P26 from AcMNPV can degrade 2'3'-cGAMP, thus inhibiting the antiviral response and improving gene delivery efficiency. Incorporating P26 into baculoviral vectors significantly increased the efficiency of transgene expression.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Noemi Esteras, Thomas S. Blacker, Evgeny A. Zherebtsov, Olga A. Stelmashuk, Ying Zhang, W. Christian Wigley, Michael R. Duchen, Albena T. Dinkova-Kostova, Andrey Y. Abramov
Summary: The transcription factor Nrf2 and its repressor Keap1 play a crucial role in cell stress adaptation by regulating gene expression related to cellular detoxification, antioxidant defense, and energy metabolism. This study investigated the impact of Nrf2 on glucose distribution and the connection between NADH production in energy metabolism and NADPH homeostasis using glio-neuronal cultures. The findings showed that Nrf2 activation enhances glucose uptake in neurons and astrocytes, with prioritized consumption for mitochondria-related energy production rather than NADPH synthesis in the pentose phosphate pathway.
Article
Biochemistry & Molecular Biology
Andrey Y. Vinokurov, Olga A. Stelmashuk, Polina A. Ukolova, Evgeny A. Zherebtsov, Andrey Y. Abramov
Summary: The brain produces various reactive oxygen species at different rates in different brain regions, with the brain stem and cerebellum being more sensitive to oxidative stress. Mitochondrial ROS has minor implications to total ROS production, and levels of lipid peroxidation and GSH vary across brain regions.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Cell Biology
Fei Liu, Yijie Zhang, Fuliang Chen, Jiacheng Yuan, Sanlan Li, Sue Han, Dengyu Lu, Junlan Geng, Zhiping Rao, Li Sun, Jianhua Xu, Yuhan Shi, Xiaojing Wang, Yueguang Liu
Summary: The study utilized the AAV(2/8) delivery system to overexpress Neurog2 into astrocytes, successfully converting them into neurons with mature electrophysiological properties, glutamatergic identity, and synapse-like configuration. Furthermore, evidence also proved that Neurog2-iNs in the intact spinal cord are capable of responding to diverse afferent inputs.
CELL DEATH & DISEASE
(2021)
Article
Multidisciplinary Sciences
Benjamin Grieb, Sivaranjan Uppala, Gal Sapir, David Shaul, J. Moshe Gomori, Rachel Katz-Brull
Summary: This study demonstrates that monitoring cerebral metabolism using hyperpolarized C-13-labeled metabolites can be used as a reporter of neural activity, with PDH activity serving as an indicator. The findings show a decrease in PDH activity when neuronal firing and ATP synthase are inhibited, providing a potential new approach for utilizing magnetic resonance imaging to monitor neural activity.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Yantao Ma, Handan Xie, Xiaomin Du, Lipeng Wang, Xueqin Jin, Qianqian Zhang, Yawen Han, Shicheng Sun, Longteng Wang, Xiang Li, Changjiang Zhang, Mengdi Wang, Cheng Li, Jun Xu, Zhuo Huang, Xiaoqun Wang, Zhen Chai, Hongkui Deng
Summary: This study demonstrates that small molecules can reprogram astrocytes into neurons in the adult mouse brain. The in situ chemically induced neurons show similarities to endogenous neurons in terms of marker expression, electrophysiological properties, and synaptic connectivity, providing a potential approach for developing neuronal replacement therapies.
Review
Biochemistry & Molecular Biology
Ismael Gonzalez-Garcia, Cristina Garcia-Caceres
Summary: Astrocytes are functionally integrated into neuronal regulatory circuits for metabolic control, particularly in the hypothalamus, where early cellular rearrangements may play a key role in the pathogenesis of obesity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Neurosciences
Foteini-Dionysia Koufi, Irene Neri, Giulia Ramazzotti, Isabella Rusciano, Sara Mongiorgi, Maria Vittoria Marvi, Antonietta Fazio, Minkyung Shin, Yoichi Kosodo, Ilaria Cani, Elisa Giorgio, Pietro Cortelli, Lucia Manzoli, Stefano Ratti
Summary: Lamin B1 plays a crucial role in brain development and aging. Its levels are important for normal brain development and function. Abnormal levels of Lamin B1 can lead to neurodegenerative disorders.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Neurosciences
Hongjie Wang, Dinesh Devadoss, Madhavan Nair, Hitendra S. Chand, Madepalli K. Lakshmana
Summary: Alzheimer's disease is a complex and heterogeneous disease, and the IQCK gene has been identified as a potential risk gene. This study found that IQCK is expressed in neurons and other brain cells, but not in microglial cells. Moreover, the protein levels of IQCK were increased in AD brains compared to normal brains, and IQCK was found in amyloid plaques.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Savina Apolloni, Martina Milani, Nadia D'Ambrosi
Summary: Friedreich's ataxia (FRDA) is a rare genetic disorder caused by mutations in the frataxin gene, leading to progressive nervous system damage. In addition to neurons, alterations in glial cells and neuroinflammatory-related mechanisms may contribute to the pathogenesis of FRDA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Clinical Neurology
Asvin K. K. Lakkaraju, Silvia Sorce, Assunta Senatore, Mario Nuvolone, Jingjing Guo, Petra Schwarz, Rita Moos, Pawel Pelczar, Adriano Aguzzi
Summary: Prion infections cause derangement in glial cells but not in neurons. Expressing PrP(C) selectively in neurons and astrocytes of mice, we found that prion accumulation and disease occurred in neuron-restricted expression, while astrocyte-restricted expression did not lead to clinical disease or inflammation. This suggests a nonautonomous mechanism in which prion-infected neurons instruct astrocytes and microglia to drive neural dysfunction.
Article
Multidisciplinary Sciences
Xiao Zhang, Yao Liu, Xiaoqi Hong, Xia Li, Charles K. Meshul, Cynthia Moore, Yabing Yang, Yanfei Han, Wei-Guang Li, Xin Qi, Huifang Lou, Shumin Duan, Tian-Le Xu, Xiaoping Tong
Summary: NG2 glia can sense synaptic inputs from neurons, forming functional GABAergic synapses with adjacent hippocampal interneurons. Activation of NG2 glia induces anxiety-like behavior in a mouse model of chronic social defeat stress.
NATURE COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Jiao Yao, Cong Chen, Yi Guo, Yantao Yang, Xinya Liu, Shifeng Chu, Qidi Ai, Zhao Zhang, Meiyu Lin, Songwei Yang, Naihong Chen
Summary: Depression has a negative impact on physical and mental health, and the exact pathophysiology is still unknown. The focus of research has shifted to the interactions between astrocytes, neurons, and depression. This review summarizes the pathological changes of neurons and astrocytes, and their interactions in depression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biophysics
Ahmed El-Serafi, Rui He, Wenyi Zheng, Fadwa Benkossou, Sandra Oerther, Ying Zhao, Karin Mellgren, Britt Gustafsson, Carsten Heilmann, Jukka Kanerva, Kourosh Lotfi, Jacek Toporski, Mikael Sundin, Martin Hoglund, Jonas Mattsson, Ibrahim El-Serafi, Moustapha Hassan
Summary: Vitamin D levels can affect oral busulphan pharmacokinetics in children, but do not have a significant impact on adults or children receiving intravenous busulphan. Further studies are needed to confirm the underlying mechanisms.
BONE MARROW TRANSPLANTATION
(2021)
Review
Chemistry, Multidisciplinary
Carlos Sanchez-Cano, Ramon A. Alvarez-Puebla, John M. Abendroth, Tobias Beck, Robert Blick, Yuan Cao, Frank Caruso, Indranath Chakraborty, Henry N. Chapman, Chunying Chen, Bruce E. Cohen, Andre L. C. Conceicao, David P. Cormode, Daxiang Cui, Kenneth A. Dawson, Gerald Falkenberg, Chunhai Fan, Neus Feliu, Mingyuan Gao, Elisabetta Gargioni, Claus-C Glueer, Florian Gruener, Moustapha Hassan, Yong Hu, Yalan Huang, Samuel Huber, Nils Huse, Yanan Kang, Ali Khademhosseini, Thomas F. Keller, Christian Koernig, Nicholas A. Kotov, Dorota Koziej, Xing-Jie Liang, Beibei Liu, Sijin Liu, Yang Liu, Ziyao Liu, Luis M. Liz-Marzan, Xiaowei Ma, Andres Machicote, Wolfgang Maison, Adrian P. Mancuso, Saad Megahed, Bert Nickel, Ferdinand Otto, Cristina Palencia, Sakura Pascarelli, Arwen Pearson, Oula Penate-Medina, Bing Qi, Joachim Raedler, Joseph J. Richardson, Axel Rosenhahn, Kai Rothkamm, Michael Rubhausen, Milan K. Sanyal, Raymond E. Schaak, Heinz-Peter Schlemmer, Marius Schmidt, Oliver Schmutzler, Theo Schotten, Florian Schulz, A. K. Sood, Kathryn M. Spiers, Theresa Staufer, Dominik M. Stemer, Andreas Stierle, Xing Sun, Gohar Tsakanova, Paul S. Weiss, Horst Weller, Fabian Westermeier, Ming Xu, Huijie Yan, Yuan Zeng, Ying Zhao, Yuliang Zhao, Dingcheng Zhu, Ying Zhu, Wolfgang J. Parak
Summary: X-ray-based analytics are widely used in physics, chemistry, materials science, and engineering, but their full potential in life sciences and medicine has not been fully utilized. Current and upcoming advances in applying these techniques to investigate the nano-bio interface are discussed, along with addressing current limitations and potential strategies for future in vivo use.
Article
Oncology
Esther Schoutrop, Ibrahim El-Serafi, Thomas Poiret, Ying Zhao, Okan Gultekin, Rui He, Lidia Moyano-Galceran, Joseph W. Carlson, Kaisa Lehti, Moustapha Hassan, Isabelle Magalhaes, Jonas Mattsson
Summary: The study demonstrates the therapeutic potential of MSLN-CAR T cells against ovarian cancer, despite challenges in achieving persistent tumor control. The findings highlight the importance of immune checkpoint pathways in limiting CAR T-cell persistence in the ovarian tumor microenvironment.
Article
Pharmacology & Pharmacy
Stefan Gruden, Charlott Brunmark, Bo Holmqvist, Erwin D. Brenndorfer, Martin Johansson, Jian Liu, Ying Zhao, Niklas Axen, Moustapha Hassan
Summary: The study evaluated the ability of calcium sulfate based NanoZolid drug delivery technology to release EGF protein while maintaining its biological activity. In vivo imaging showed controlled release of EGF-NIR loaded in NanoZolid depots compared to free EGF-NIR, with predominant distribution in regions with high EGFR expression levels. This technology has potential for enhancing treatment efficacy and minimizing systemic adverse effects.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Meeting Abstract
Hematology
Moustapha Hassan, Per Ljungman, Rui He, Svante Norgren, Wenyi Zheng, Ying Zhao, Agneta Mansson-Broberg, Eva Wardell, Terra Slof
Review
Radiology, Nuclear Medicine & Medical Imaging
Qiang Wang, Anrong Wang, Ernesto Sparrelid, Jiaxing Zhang, Ying Zhao, Kuansheng Ma, Torkel B. Brismar
Summary: Evaluation of gadoxetic acid-enhanced MRI as an imaging biomarker for predicting PHLF showed promising results with varying predictive sensitivity and specificity. Majority of studies indicated that the gadoxetic acid-enhanced MRI parameters were predictors for PHLF. Further multicenter, prospective trials with larger sample sizes are needed to validate the efficacy of individual liver function parameters.
EUROPEAN RADIOLOGY
(2022)
Article
Gastroenterology & Hepatology
Qiang Wang, Savas Kesen, Maria Liljeroth, Henrik Nilsson, Ying Zhao, Ernesto Sparrelid, Torkel B. Brismar
Summary: This study compared the efficacy of three types of gadoxetic acid enhanced MR parameters for quantitative assessment of liver function. The results showed that simple signal intensity-based parameters were as effective as more complex parameters in evaluating liver function. The liver-to-muscle ratio was identified as the easiest-to-use parameter for quantitative evaluation of liver function in clinical practice.
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
(2022)
Article
Oncology
Xiaoli Li, Duanfang Zhou, Yongqing Cai, Xiaoping Yu, Xiangru Zheng, Bo Chen, Wenjun Li, Hongfang Zeng, Moustapha Hassan, Ying Zhao, Weiying Zhou
Summary: This study found that ER stress decreased the expression level of AR in LAR TNBC and PCa through the regulation of PERK/eIF2 alpha/ATF4 signaling pathway, thereby inhibiting tumor proliferation.
Article
Health Care Sciences & Services
Mona Fares, Sandra Oerther, Kjell Hultenby, Danica Gubrianska, Ying Zhao, Manuchehr Abedi-Valugerdi, Moustapha Hassan
Summary: This study investigated the mechanisms of COL-3-induced cytotoxicity in a chronic myeloid leukemia cell line, revealing that it involves DNA damage, mitochondrial and ER perturbation, and features of paraptosis and programmed necrosis.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Cell Biology
Xiuming Liang, Zheyu Niu, Valentina Galli, Nathalie Howe, Ying Zhao, Oscar P. B. Wiklander, Wenyi Zheng, Rim Jawad Wiklander, Giulia Corso, Christopher Davies, Justin Hean, Eleni Kyriakopoulou, Doste R. Mamand, Risul Amin, Joel Z. Nordin, Dhanu Gupta, Samir E. L. Andaloussi
Summary: This study explores an engineering strategy to prolong the circulation time of extracellular vesicles (EVs) by decorating the surface with albumin. The engineered EVs demonstrate strong binding capacity and significantly increased circulation time after injection in mice. Furthermore, they accumulate in lymph nodes and solid tumors, which can be utilized for immunomodulation, cancer therapy, and immunotherapy.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Review
Health Care Sciences & Services
Ying Zhao, Rui He, Sandra Oerther, Weiying Zhou, Massoud Vosough, Moustapha Hassan
Summary: Hematopoietic stem cell transplantation (HSCT) is the only curative treatment for various hematologic malignancies, solid tumors, inborn errors of metabolism or genetic disorders. However, treatment-related complications, including graft-versus-host disease (GvHD) and cardiovascular complications (CVC), still affect clinical outcomes. Evaluating and treating cardiac function before and after HSCT is crucial for improving patient care.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Plant Sciences
Duanfang Zhou, Xiaoli Li, Xiaoqiu Xiao, Gang Wang, Bo Chen, Yi Song, Xu Liu, Qichen He, Huan Zhang, Qiuya Wu, Limei Zhang, Lihong Wu, Zhengze Shen, Moustapha Hassan, Ying Zhao, Weiying Zhou
Summary: Celastrol was found to effectively improve type 2 diabetes through targeting the ChREBP-TXNIP axis. It directly bound to ChREBP, inhibiting its nuclear translocation and promoting proteasomal degradation, leading to the repression of TXNIP transcription and ultimately ameliorating T2DM.
Article
Oncology
Esther Schoutrop, Thomas Poiret, Ibrahim El-Serafi, Ying Zhao, Rui He, Alina Moter, Johan Henriksson, Moustapha Hassan, Isabelle Magalhaes, Jonas Mattsson
Summary: This study demonstrates the enhanced therapeutic potential of MSLN-CAR T cells expressing a mutated CD3 zeta chain containing a single ITAM for the treatment of ovarian cancer. CAR T cells with calibrated activation potential may improve clinical responses in solid tumors.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Nanoscience & Nanotechnology
Ksenia Loskutova, Mar Torras, Ying Zhao, Anna J. Svagan, Dmitry Grishenkov
Summary: The aim of this study was to investigate the effect of cellulose nanofiber (CNF)-shelled perfluoropentane (PFP) droplets on the cell viability of 4T1 breast cancer cells, with or without the addition of non-encapsulated paclitaxel. CNF-shelled PFP droplets were produced and their size distribution and concentration were analyzed. The thermal stability and blood compatibility of CNF-shelled PFP droplets were also evaluated. The results showed that CNF-shelled PFP droplets have the potential to be used as drug carriers in ultrasound-mediated therapy as they did not affect cell viability and displayed no hemolysis.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Multidisciplinary Sciences
Wenyi Zheng, Julia Raedler, Helena Sork, Zheyu Niu, Samantha Roudi, Jeremy P. P. Bost, Andre Goergens, Ying Zhao, Doste R. R. Mamand, Xiuming Liang, Oscar P. B. Wiklander, Taavi Lehto, Dhanu Gupta, Joel Z. Z. Nordin, Samir EL Andaloussi
Summary: This study conducted a large-scale screening to identify potential scaffold proteins for loading therapeutic cargo into extracellular vesicles (EVs). They found 24 proteins with conserved EV-sorting abilities, including TSPAN2 and TSPAN3 which outperformed the well-studied CD63 scaffold. These engineered EVs showed promise as delivery vehicles in cell cultures and mice.
NATURE COMMUNICATIONS
(2023)