Article
Biochemistry & Molecular Biology
Niels Boegholm, Narcis A. Petriman, Marta Loureiro-Lopez, Jiaolong Wang, Miren Itxaso Santiago Vela, Beibei Liu, Tomoharu Kanie, Roy Ng, Peter K. Jackson, Jens S. Andersen, Esben Lorentzen
Summary: Cilia are important cellular organelles for signaling and motility and are constructed via intraflagellar transport (IFT). RabL2 localizes to the basal body of cilia via an interaction with CEP19 before downstream association with the IFT machinery. Reconstituted pentameric IFT complex containing IFT81/74 enhances the GTP hydrolysis rate of RabL2. Structural models of RabL2-containing IFT complexes validate the architectural understanding of RabL2 incorporation into the IFT complex.
Review
Biochemistry & Molecular Biology
Francesca Finetti, Anna Onnis, Cosima T. Baldari
Summary: Initially discovered as part of the intraflagellar transport (IFT) system, IFT20 protein is now known to play a role in various unconventional mechanisms. It is important not only in ciliogenesis, but also in vesicular trafficking of membrane receptors and signaling proteins. Its ability to interact with different cellular partners in a cell-type specific manner expands its function to the regulation of intracellular degradative and secretory pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Narcis A. Petriman, Marta Loureiro-Lopez, Michael Taschner, Nevin K. Zacharia, Magdalena M. Georgieva, Niels Boegholm, Jiaolong Wang, Andre Mourao, Robert B. Russell, Jens S. Andersen, Esben Lorentzen
Summary: Cilia are essential organelles for cellular functions such as motility, signaling, and sensory reception. In this study, the researchers used Alphafold to model the structure of the IFT-B complex, which is involved in cilium formation. The results provide insights into the assembly and function of the IFT-B complex.
Review
Cell Biology
Laura Sanchez-Bellver, Vasileios Toulis, Gemma Marfany
Summary: Ciliopathies are a group of heterogeneous inherited disorders caused by dysfunction of the cilium, affecting cells in multiple organs. The unique feature of daily renewal in photoreceptor outer segments and the efficient transport of proteins in ciliary transport are crucial for photoreceptor survival.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Physiology
Kelly M. DeMars, Madeleine R. Ross, Alana Starr, Jeremy C. McIntyre
Summary: Neuronal primary cilia play a critical role in central regulation of energy homeostasis. These microtubule-based organelles serve as signaling antennae for metabolic status and impaired ciliary function can lead to obesity-related disorders. In addition to metabolic signaling, primary cilia also modulate catecholamine neuromodulation and shape neuronal circuitry. This review aims to highlight current research on the mechanisms of primary cilium-regulated metabolic drives for maintaining energy homeostasis.
FRONTIERS IN PHYSIOLOGY
(2023)
Review
Andrology
Yong-Hong Man, Isabella Warmbrunn, Ling Zhang, Zhi-Bing Zhang
Summary: This review discusses the molecular functions of intraflagellar transport (IFT) protein IFT25 and its interactions with other IFT particle subunits, and how they are involved in mammalian development and fertility.
ASIAN JOURNAL OF ANDROLOGY
(2022)
Article
Multidisciplinary Sciences
David Bauer, Hiroaki Ishikawa, Kimberly A. Wemmer, Nathan L. Hendel, Jane Kondev, Wallace F. Marshall
Summary: Cell-to-cell variation is mainly influenced by cell size, while within-cell variation is caused by dynamic fluctuations. Tubulin assembly is not directly related to intraflagellar transport, and length fluctuations reflect tubulin assembly and disassembly events involving large numbers of tubulin dimers. Length variation increases in long-flagella mutants, consistent with theoretical models for flagellar length regulation.
Editorial Material
Biochemistry & Molecular Biology
Takashi Ishikawa
Summary: Two studies have revealed the atomic structure of IFT-B, showcasing the arrangement of its component proteins and the dynamic interplay between them, using cutting-edge technology.
Review
Cell Biology
Dinu Antony, Han G. Brunner, Miriam Schmidts
Summary: The function of cilia is crucial for vertebrate development and health, and dysfunction can lead to various ciliopathies. Major human ciliopathy cases are caused by malfunction of ciliary dynein motor activity, with different types of dynein dysfunction resulting in different disease phenotypes.
Article
Multidisciplinary Sciences
Alexander Ewerling, Vanessa Maissl, Bill Wickstead, Helen Louise May-Simera
Summary: The BBSome, a transport complex in cilia, also has nuclear functions. These functions are not restricted to mammals and may be a common feature in eukaryotes.
Article
Endocrinology & Metabolism
Clarissa R. Coveney, Hasmik J. Samvelyan, Jadwiga Miotla-Zarebska, Josephine Carnegie, Emer Chang, C. Jonty Corrin, Trystan Coveney, Bryony Stott, Ida Parisi, Claudia Duarte, Tonia L. Vincent, Katherine A. Staines, Angus K. T. Wann
Summary: Compared with endochondral ossification, the coordinated arrest of growth defined by the narrowing and fusion of the cartilaginous growth plate is less understood. The primary cilia play a protective role in the ossification of the growth plate during adolescent skeletal maturation, and the absence of primary cilia disrupts chondrocyte differentiation, cartilage resorption, and mineralization, primarily in load-bearing regions.
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Yincong Zhang, Xiaona Zhang, Yumin Dai, Mengjiao Song, Yifei Zhou, Jun Zhou, Xiumin Yan, Yidong Shen
Summary: The study reveals that the decrease of intraflagellar transport in the cilia of sensory neurons impairs sensory perception and metabolism in ageing C. elegans.
NATURE COMMUNICATIONS
(2021)
Review
Cell Biology
Bo Wang, Zheyong Liang, Peijun Liu
Summary: This article reviews the role of the primary cilium in cancer, including its architecture, signaling pathways, cilia assembly and disassembly regulators, summarizing new findings in tumor microenvironments and different cancers, highlighting novel possibilities for therapeutic targets in cancer.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Wei-Yue Sun, Bin Xue, Yan-Xia Liu, Rui-Kai Zhang, Rong-Chao Li, Wen Xin, Mingfu Wu, Zhen-Chuan Fan
Summary: LZTFL1 maintains BBSome dynamics in cilia by controlling BBSome recruitment to the basal body and its reassembly at the ciliary tip, thus regulating ciliary signaling.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Augustine Cleetus, Georg Merck, Felix Mueller-Planitz, Zeynep Oktena
Summary: This article introduces the recognition and transport mechanism of cellular cargo in eukaryotic cells, and the research results of this mechanism in Caenorhabditis elegans and Tetrahymena thermophila. The study found that the role of homodimeric kinesin-2 in intraflagellar transport (IFT) is more important than previously thought, and it shares the same activation mechanism in distant organisms with homodimeric kinesin-2.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Florian A. Siebzehnrubl, Kerstin A. Reber, Yvonne K. Urbach, Anja Schulze-Krebs, Fabio Canneva, Sandra Moceri, Johanna Habermeyer, Dalila Achoui, Bhavana Gupta, Dennis A. Steindler, Michael Stephan, Huu Phuc Nguyen, Michael Bonin, Olaf Riess, Andreas Bauer, Ludwig Aigner, Sebastien Couillard-Despres, Martin Arce Paucar, Per Svenningsson, Alexander Osmand, Alexander Andreew, Claus Zabel, Andreas Weiss, Rainer Kuhn, Saliha Moussaoui, Ines Blockx, Annemie Van der Linden, Rachel Y. Cheong, Laurent Roybon, Asa Petersen, Stephan von Hoersten
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Biochemistry & Molecular Biology
Lan B. Hoang-Minh, Florian A. Siebzehnrubl, Changlin Yang, Silveli Suzuki-Hatano, Kyle Dajac, Tyler Loche, Nicholas Andrews, Michael Schmoll Massari, Jaimin Patel, Krisha Amin, Alvin Vuong, Ana Jimenez-Pascual, Paul Kubilis, Timothy J. Garrett, Craig Moneypenny, Christina A. Pacak, Jianping Huang, Elias J. Sayour, Duane A. Mitchell, Matthew R. Sarkisian, Brent A. Reynolds, Loic P. Deleyrolle
Review
Cell Biology
Ana Jimenez-Pascual, Florian A. Siebzehnrubl
Article
Oncology
Ana Jimenez-Pascual, James S. Hale, Anja Kordowski, Jamie Pugh, Daniel J. Silver, Defne Bayik, Gustavo Roversi, Tyler J. Alban, Shilpa Rao, Rui Chen, Thomas M. McIntyre, Giorgio Colombo, Giulia Taraboletti, Karl O. Holmberg, Karin Forsberg-Nilsson, Justin D. Lathia, Florian A. Siebzehnrubl
Meeting Abstract
Oncology
Jo Brown, Malik Zaben, Florian Siebzehnrubl, William P. Gray
Review
Pharmacology & Pharmacy
Ana Jimenez-Pascual, Kelly Mitchell, Florian A. Siebzehnrubl, Justin D. Lathia
EXPERT OPINION ON THERAPEUTIC TARGETS
(2020)
Article
Cell Biology
Bhavana Gupta, Adam C. Errington, Ana Jimenez-Pascual, Vasileios Eftychidis, Simone Brabletz, Marc P. Stemmler, Thomas Brabletz, David Petrik, Florian A. Siebzehnrubl
Summary: The study reveals that the transcription factor ZEB1 plays a crucial role in the self-renewal of active RGL cells in the adult mammalian hippocampus, with its deletion leading to a shift towards symmetric cell division and an increase in newborn neurons while decreasing newly generated astrocytes. ZEB1 is identified as a positive regulator of the ets-domain transcription factor ETV5, which is essential for asymmetric division.
Article
Cell Biology
David Petrik, Sara Jorgensen, Vasileios Eftychidis, Florian A. Siebzehnrubl
Summary: This study explores the heterogeneity of adult neural stem cells, including different types of short-term and long-term self-renewing aNSCs. It also investigates regional and temporal differences in aNSC function, as well as single cell transcriptomics.
Article
Virology
Emily A. Bates, Charlotte Lovatt, Alice R. Plein, James A. Davies, Florian A. Siebzehnrubl, Alan L. Parker
Summary: Glioblastoma (GBM) is a common and aggressive adult brain cancer with a low survival rate. Adenovirus-based gene therapy shows potential as an alternative treatment for GBM. By pseudotyping an HAdV-C5-based platform, we demonstrate that adenoviral entry receptors CAR, CD46, and DSG2 effectively transduce GBM cells. However, the presence of these receptors on healthy cells raises concerns about off-target effects and therapeutic gene expression in non-transformed cells.
Article
Chemistry, Analytical
Iestyn Pope, Francesco Masia, Kenneth Ewan, Ana Jimenez-Pascual, Trevor C. Dale, Florian A. Siebzehnrubl, Paola Borri, Wolfgang Langbein
Summary: Quantitative hyperspectral coherent Raman scattering microscopy combines imaging with spectroscopy to extract physically meaningful chemical components with sub-cellular resolution. This label-free method can discriminate different cellular sub-populations and has potential for non-invasive disease diagnostics.
Meeting Abstract
Radiology, Nuclear Medicine & Medical Imaging
R. L. Smith, N. Evans, V. Florence, S. Paisey, E. Fittock, F. Siebzehnrubl, C. Marshall
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2019)
Meeting Abstract
Neurosciences
B. Gupta, G. Berx, S. Brabletz, T. Brabletz, M. Stemmler, F. A. Siebzehnrubl
Meeting Abstract
Oncology
Ana Jimenez-Pascual, James Hale, Anja Kordowski, Jamie Pugh, Shilpa Rao, Daniel Silver, Tyler Alban, Defne Watson, Rui Chen, Thomas McIntyre, Giorgio Colombo, Giulia Taraboletti, Karl Holmberg, Karin Forsberg-Nilsson, Justin Lathia, Florian Siebzehnrubl
Meeting Abstract
Radiology, Nuclear Medicine & Medical Imaging
S. J. Paisey, E. Fittock, B. Gorell, T. Caws, V. Marsh-Durban, R. Corteling, A. Dabkowski, C. Marshall, A. Clayton, F. Siebzehnrubl
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2018)
Meeting Abstract
Radiology, Nuclear Medicine & Medical Imaging
R. L. Smith, V. Florence, S. Paisey, E. Fittock, F. Siebzehnrubl, E. Spezi, C. Marshall
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2018)