Biochemically validated structural model of the 15‐subunit intraflagellar transport complex IFT‐B

Chlamydomonas ARMC2/PF27 is an obligate cargo adapter for intraflagellar transport of radial spokes

(2022) Karl F Lechtreck et al.   eLife

ColabFold: making protein folding accessible to all

(2022) Milot Mirdita et al.   NATURE METHODS

DYF-5/MAK–dependent phosphorylation promotes ciliary tubulin unloading

(2022) Xuguang Jiang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In situ architecture of the ciliary base reveals the stepwise assembly of intraflagellar transport trains

(2022) Hugo van den Hoek et al.   SCIENCE

Benchmarking the Accuracy of AlphaFold 2 in Loop Structure Prediction

(2022) Amy O. Stevens et al.   Biomolecules

Mapping protein-protein interactions in homodimeric CYP102A1 by crosslinking and mass spectrometry

(2021) Dana Felker et al.   BIOPHYSICAL CHEMISTRY

Highly accurate protein structure prediction with AlphaFold

(2021) John Jumper et al.   NATURE

Ciliary Dyneins and Dynein Related Ciliopathies

(2021) Dinu Antony et al.   Cells

Rab‐like small GTPases in the regulation of ciliary Bardet‐Biedl syndrome (BBS) complex transport

(2021) Xiumin Yan et al.   FEBS Journal

Impaired cooperation between IFT74/BBS22–IFT81 and IFT25–IFT27/BBS19 causes Bardet-Biedl syndrome

(2021) Zhuang Zhou et al.   HUMAN MOLECULAR GENETICS

The PRIDE database resources in 2022: a hub for mass spectrometry-based proteomics evidences

(2021) Yasset Perez-Riverol et al.   NUCLEIC ACIDS RESEARCH

Transport of Ciliary Membrane Proteins

(2020) Huan Long et al.   Frontiers in Cell and Developmental Biology

Ubiquitin links smoothened to intraflagellar transport to regulate Hedgehog signaling

(2020) Paurav B. Desai et al.   JOURNAL OF CELL BIOLOGY

Purification and crystal structure of human ODA16 : Implications for ciliary import of outer dynein arms by the intraflagellar transport machinery

(2020) Jiaolong Wang et al.   PROTEIN SCIENCE

Structural Characteristics, Binding Partners and Related Diseases of the Calponin Homology (CH) Domain

(2020) Lei-Miao Yin et al.   Frontiers in Cell and Developmental Biology

Diffusion rather than intraflagellar transport likely provides most of the tubulin required for axonemal assembly in Chlamydomonas

(2020) Julie Craft Van De Weghe et al.   JOURNAL OF CELL SCIENCE

Identification and Characterization of Known Biallelic Mutations in the IFT27 (BBS19) Gene in a Novel Family With Bardet-Biedl Syndrome

(2019) Elise Schaefer et al.   Frontiers in Genetics

Protein aggregation capture on microparticles enables multi-purpose proteomics sample preparation.

(2019) Tanveer Singh Batth et al.   MOLECULAR & CELLULAR PROTEOMICS

Binding of IFT22 to the intraflagellar transport complex is essential for flagellum assembly

(2019) Stefanie Wachter et al.   EMBO JOURNAL

Human IFT52 mutations uncover a novel role for the protein in microtubule dynamics and centrosome cohesion

(2019) Marie Alice Dupont et al.   HUMAN MOLECULAR GENETICS

A Simple Cross-Linking/Mass Spectrometry Workflow for Studying System-wide Protein Interactions

(2019) Michael Götze et al.   ANALYTICAL CHEMISTRY

Namdinator – automatic molecular dynamics flexible fitting of structural models into cryo-EM and crystallography experimental maps

(2019) Rune Thomas Kidmose et al.   IUCrJ

Structure of the dynein-2 complex and its assembly with intraflagellar transport trains

(2019) Katerina Toropova et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Loss of function IFT27 variants associated with an unclassified lethal fetal ciliopathy with renal agenesis

(2018) Chloé Quélin et al.   AMERICAN JOURNAL OF MEDICAL GENETICS PART A

Interaction of heterotrimeric kinesin-II with IFT-B–connecting tetramer is crucial for ciliogenesis

(2018) Teruki Funabashi et al.   JOURNAL OF CELL BIOLOGY

Crystal structure of intraflagellar transport protein 80 reveals a homo-dimer required for ciliogenesis

(2018) Michael Taschner et al.   eLife

Robust interaction of IFT70 with IFT52–IFT88 in the IFT-B complex is required for ciliogenesis

(2018) Ryota Takei et al.   Biology Open

The cryo-EM structure of intraflagellar transport trains reveals how dynein is inactivated to ensure unidirectional anterograde movement in cilia

(2018) Mareike A. Jordan et al.   NATURE CELL BIOLOGY

A cross-linking/mass spectrometry workflow based on MS-cleavable cross-linkers and the MeroX software for studying protein structures and protein–protein interactions

(2018) Claudio Iacobucci et al.   Nature Protocols

Membrane association and remodeling by intraflagellar transport protein IFT172

(2018) Qianmin Wang et al.   Nature Communications

Structural basis of outer dynein arm intraflagellar transport by the transport adaptor protein ODA16 and the intraflagellar transport protein IFT46

(2017) Michael Taschner et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The N-terminus of IFT46 mediates intraflagellar transport of outer arm dynein and its cargo-adaptor ODA16

(2017) Yuqing Hou et al.   MOLECULAR BIOLOGY OF THE CELL

RABL2 interacts with the intraflagellar transport-B complex and CEP19 and participates in ciliary assembly

(2017) Yuya Nishijima et al.   MOLECULAR BIOLOGY OF THE CELL

IFT trains in different stages of assembly queue at the ciliary base for consecutive release into the cilium

(2017) Jenna L Wingfield et al.   eLife

Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline

(2017) Martha E. Brennich et al.   Jove-Journal of Visualized Experiments

A homozygous nonsense variant inIFT52is associated with a human skeletal ciliopathy

(2016) K. M. Girisha et al.   CLINICAL GENETICS

The intraflagellar transport machinery in ciliary signaling

(2016) André Mourão et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Intraflagellar transport proteins 172, 80, 57, 54, 38, and 20 form a stable tubulin‐binding IFT‐B2 complex

(2016) Michael Taschner et al.   EMBO JOURNAL

IFT52mutations destabilize anterograde complex assembly, disrupt ciliogenesis and result in short rib polydactyly syndrome

(2016) Wenjuan Zhang et al.   HUMAN MOLECULAR GENETICS

Overall Architecture of the Intraflagellar Transport (IFT)-B Complex Containing Cluap1/IFT38 as an Essential Component of the IFT-B Peripheral Subcomplex

(2016) Yohei Katoh et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Together, the IFT81 and IFT74 N-termini form the main module for intraflagellar transport of tubulin

(2016) Tomohiro Kubo et al.   JOURNAL OF CELL SCIENCE

Microtubule doublets are double-track railways for intraflagellar transport trains

(2016) L. Stepanek et al.   SCIENCE

The Intraflagellar Transport Machinery

(2016) Michael Taschner et al.   Cold Spring Harbor Perspectives in Biology

Ciliopathies

(2016) Daniela A. Braun et al.   Cold Spring Harbor Perspectives in Biology

An organelle-specific protein landscape identifies novel diseases and molecular mechanisms

(2016) Karsten Boldt et al.   Nature Communications

Assembly of IFT Trains at the Ciliary Base Depends on IFT74

(2015) Jason M. Brown et al.   CURRENT BIOLOGY

Getting tubulin to the tip of the cilium: One IFT train, many different tubulin cargo-binding sites?

(2014) Sagar Bhogaraju et al.   BIOESSAYS

The Intraflagellar Transport Protein IFT27 Promotes BBSome Exit from Cilia through the GTPase ARL6/BBS3

(2014) Gerald M. Liew et al.   DEVELOPMENTAL CELL

IFT27 Links the BBSome to IFT for Maintenance of the Ciliary Signaling Compartment

(2014) Thibaut Eguether et al.   DEVELOPMENTAL CELL

IFT27, encoding a small GTPase component of IFT particles, is mutated in a consanguineous family with Bardet-Biedl syndrome

(2014) M. A. Aldahmesh et al.   HUMAN MOLECULAR GENETICS

Crystal structures of IFT70/52 and IFT52/46 provide insight into intraflagellar transport B core complex assembly

(2014) Michael Taschner et al.   JOURNAL OF CELL BIOLOGY

Automated Assignment of MS/MS Cleavable Cross-Links in Protein 3D-Structure Analysis

(2014) Michael Götze et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

A Mutation in the Mouse Ttc26 Gene Leads to Impaired Hedgehog Signaling

(2014) Ruth E. Swiderski et al.   PLoS Genetics

TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagella

(2014) Hiroaki Ishikawa et al.   eLife

Defects in the IFT-B Component IFT172 Cause Jeune and Mainzer-Saldino Syndromes in Humans

(2013) Jan Halbritter et al.   AMERICAN JOURNAL OF HUMAN GENETICS

Cycling of the signaling protein phospholipase D through cilia requires the BBSome only for the export phase

(2013) Karl F. Lechtreck et al.   JOURNAL OF CELL BIOLOGY

Molecular Basis of Tubulin Transport Within the Cilium by IFT74 and IFT81

(2013) S. Bhogaraju et al.   SCIENCE

IFT25 Links the Signal-Dependent Movement of Hedgehog Components to Intraflagellar Transport

(2012) Brian T. Keady et al.   DEVELOPMENTAL CELL

New developments in theATSASprogram package for small-angle scattering data analysis

(2012) Maxim V. Petoukhov et al.   JOURNAL OF APPLIED CRYSTALLOGRAPHY

Dissecting the Sequential Assembly and Localization of Intraflagellar Transport Particle Complex B in Chlamydomonas

(2012) Elizabeth A. Richey et al.   PLoS One

Overview of theCCP4 suite and current developments

(2011) Martyn D. Winn et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Mutations in Traf3ip1 reveal defects in ciliogenesis, embryonic development, and altered cell size regulation

(2011) Nicolas F. Berbari et al.   DEVELOPMENTAL BIOLOGY

Architecture and function of IFT complex proteins in ciliogenesis

(2011) Michael Taschner et al.   DIFFERENTIATION

Crystal structure of the intraflagellar transport complex 25/27

(2011) Sagar Bhogaraju et al.   EMBO JOURNAL

Biochemical Mapping of Interactions within the Intraflagellar Transport (IFT) B Core Complex

(2011) Michael Taschner et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

XDS

(2010) Wolfgang Kabsch   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

PHENIX: a comprehensive Python-based system for macromolecular structure solution

(2010) Paul D. Adams et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Electron-tomographic analysis of intraflagellar transport particle trains in situ

(2009) Gaia Pigino et al.   JOURNAL OF CELL BIOLOGY

TheChlamydomonas reinhardtiiBBSome is an IFT cargo required for export of specific signaling proteins from flagella

(2009) Karl-Ferdinand Lechtreck et al.   JOURNAL OF CELL BIOLOGY

An Enhanced System for Unnatural Amino Acid Mutagenesis in E. coli

(2009) Travis S. Young et al.   JOURNAL OF MOLECULAR BIOLOGY

elipsa is an early determinant of ciliogenesis that links the IFT particle to membrane-associated small GTPase Rab8

(2008) Yoshihiro Omori et al.   NATURE CELL BIOLOGY