Article
Plant Sciences
Sergio M. Borghi, Talita P. Domiciano, Fernanda S. Rasquel-Oliveira, Camila R. Ferraz, Allan J. C. Bussmann, Josiane A. Vignoli, Doumit Camilios-Neto, Sergio R. Ambrosio, Nilton S. Arakawa, Rubia Casagrande, Waldiceu A. Verri
Summary: The study indicated that kaurenoic acid can prevent antigen-induced asthma by down-regulating inflammatory pathways, promoting antioxidant effects, and regulating regulatory T cell markers, explaining the ethnopharmacological use of S. trilobata for the treatment of lung inflammatory diseases.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Plant Sciences
Sergio M. Borghi, Sandra S. Mizokami, Thacyana T. Carvalho, Fernanda S. Rasquel-Oliveira, Camila R. Ferraz, Victor Fattori, Thiago H. Hayashida, Jean P. S. Peron, Doumit Camilios-Neto, Sergio R. Ambrosio, Nilton S. Arakawa, Rubia Casagrande, Waldiceu A. Verri Jr
Summary: The study demonstrated that KA can inhibit LPS-induced mechanical and thermal hyperalgesia, MPO activity, and modulate redox status in the mice paw. In LPS peritonitis, KA inhibited leukocyte migration, cytokine production, oxidative stress, and NF-κB activation, indicating its potential as a candidate for controlling inflammation and pain during gram-negative bacterial infections.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Yixuan Liu, Qi Sun, Chengfei Zhang, Min Ding, Cheng Wang, Qian Zheng, Zhijie Ma, Haojun Xu, Guoren Zhou, Xiaoming Wang, Zhangjun Cheng, Hongping Xia
Summary: The study found that modulating the function of tumor-associated macrophages is a potential therapeutic strategy to control colorectal cancer liver metastasis. Activation of STING inhibited CRC liver metastasis and affected the macrophage phenotype and function through IRG1. Thus, the STING-IRG1 axis plays a critical role in macrophage reprogramming and tumor liver metastasis.
Article
Plant Sciences
Davoud Ahmadimoghaddam, Mohammad Zarei, Saeed Mohammadi, Zohreh Izadidastenaei, Iraj Salehi
Summary: In Iranian folkloric medicine, Bupleurum falcatum L. has been used as a selective analgesic remedy. The research investigated the anti-nociceptive and anti-allodynic action of Bupleurum falcatum L. roots essential oil in Swiss mice. Results showed that BFEO exhibited significant antinociceptive effects and could effectively alleviate allodynia in CSC mice by activating specific pathways and receptors.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Bokyung Song, Sung-Joong Lee, Chong-Hyun Kim
Summary: In a ATP-induced cortical injury model, ATP and cytokine TNF-alpha significantly affect the physiological activities of neurons and microglia at day 3 post-injection, while IL-10 and IL-4 effectively suppress these changes in both cell types.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Shotaro Nakajima, Kosaku Mimura, Akinao Kaneta, Katsuharu Saito, Masanori Katagata, Hirokazu Okayama, Motonobu Saito, Zenichiro Saze, Yohei Watanabe, Hiroyuki Hanayama, Takeshi Tada, Wataru Sakamoto, Tomoyuki Momma, Hiromasa Ohira, Koji Kono
Summary: This study investigated the role of tumor cell-intrinsic cGAS-STING in radiation-mediated remodeling of the tumor microenvironment (TME) in esophageal squamous cell carcinoma (ESCC). The findings demonstrate that cGAS-STING is involved in radiation-induced activation of immune cells and recruitment of tumor-promoting M2-tumor-associated macrophages (TAMs) through IL-34. Targeting IL-34 to block M2-TAM infiltration may improve the efficacy of radiation therapy and combination therapy with immune checkpoint inhibitors in ESCC.
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2023)
Article
Multidisciplinary Sciences
Matteo Gentili, Bingxu Liu, Malvina Papanastasiou, Deborah Dele-Oni, Marc A. Schwartz, Rebecca J. Carlson, Aziz M. Al'Khafaji, Karsten Krug, Adam Brown, John G. Doench, Steven A. Carr, Nir Hacohen
Summary: In this study, genes regulating STING trafficking were systematically characterized, and their impact on STING-mediated responses was examined. The study revealed that the ESCRT complex promotes STING degradation and terminates STING signaling. Furthermore, a UBAP1 mutation was found to increase steady-state STING-dependent responses. Overall, this study uncovers the role of the ESCRT complex in regulating STING signaling.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Chang Lin, Min Zheng, Shifeng Xiao, Shao Wang, Xiaoli Zhu, Xiuqin Chen, Dandan Jiang, Xiancheng Zeng, Shaoying Chen, Shilong Chen
Summary: This study cloned the mallard (Anas platyrhynchos) cGAS gene and found that du-cGAS exhibits similar functional domains with its chicken counterpart. The du-cGAS plays an important antiviral role in the innate immunity of ducks and provides an effective strategy for controlling waterfowl viral diseases.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Keila Belhart, Federico Sisti, Monica C. Gestal, Julieta Fernandez
Summary: Bordetella bronchiseptica is a gram-negative bacterium that serves as a model for studying host-pathogen interactions. This study identifies the diguanylate cyclase BdcB as an important regulator of biofilm formation and motility in B. bronchiseptica. The absence of BdcB leads to increased cytotoxicity and pro-inflammatory response in macrophages, as well as upregulation of T3SS-mediated toxins. These findings highlight the role of BdcB in virulence regulation and host immune response in B. bronchiseptica.
SCIENTIFIC REPORTS
(2023)
Article
Physiology
Adrina Kalasho Kuzmiszyn, Anders Lund Selli, Natalia Smaglyukova, Timofei Kondratiev, Ole-Martin Fuskevag, Roy Andre Lysa, Aina Westrheim Ravna, Torkjel Tveita, Georg Sager, Erik Sveberg Dietrichs
Summary: This study aims to investigate whether PDE3 inhibitors milrinone, amrinone, and levosimendan are able to modulate cyclic nucleotide regulation in hypothermic settings. The results show that milrinone, amrinone, and levosimendan can suppress enzymatic degradation and inhibit extrusion of cGMP and cAMP below 30 degrees C, indicating that they could provide an important treatment option for hypothermia-induced cardiac dysfunction.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Microbiology
Adrianne N. Edwards, Caitlin L. Willams, Nivedita Pareek, Shonna M. McBride, Rita Tamayo
Summary: The study demonstrated that cyclic diguanylate (c-di-GMP) negatively impacts sporulation in C. difficile, suggesting that c-di-GMP likely controls early sporulation events through an unidentified regulatory pathway. This work implicates c-di-GMP metabolism as a mechanism to integrate environmental and intracellular cues to influence C. difficile sporulation.
Article
Pharmacology & Pharmacy
Plinio Bezerra Palacio, Aline Maria Brito Lucas, Joana Varlla de Lacerda Alexandre, Pedro Lourenzo Oliveira Cunha, Yuana Ivia Ponte Viana, Amanda Cabral Albuquerque, Anna Lidia Nunes Varela, Heberty Tarso Facundo
Summary: The study reveals competitive binding of glibenclamide and diazoxide at mitoSUR, with the cyclohexylurea portion of glibenclamide being essential for mitoKATP inhibition. IMP-A does not inhibit diazoxide-induced cardioprotection, but glibenclamide effectively inhibits it. This research provides valuable insights into the pharmacological and biochemical aspects of mitoKATP activation and cardioprotection, potentially leading to new therapeutic strategies for ischemia-reperfusion injury.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Alexander G. Tkachenko, Natalya M. Kashevarova, Roman Yu Sidorov, Larisa Yu Nesterova, Anna Akhova, Ivan Tsyganov, Vladimir Yu Vaganov, Sergei A. Shipilovskikh, Aleksandr E. Rubtsov, Andrei Malkov
Summary: A synthetic compound called DMNP has been identified as capable of suppressing persistence and eradicating biofilms in Mycobacterium smegmatis, possibly by targeting enzymes like Rel(Msm) and RelZ. This research suggests that DMNP may be a promising lead compound for the development of antimycobacterial drugs.
CELL CHEMICAL BIOLOGY
(2021)
Article
Veterinary Sciences
Xinyu Wang, Yue Wu, Lin Liu, Hui Bai, Zhiheng Zhang, Mingchao Zhao, Tianwen Ma, Xiaopeng Song, Lina Jia, Liangyu Lv, Yue Yu, Xinyu Xu, Hong Chen, Li Gao
Summary: This study provides new evidence that the anesthetic and analgesic effects of Xyl are related to the inhibition of the NO-cGMP signaling pathway.
JOURNAL OF VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Wen Zhou, Lisa Mohr, John Maciejowski, Philip J. Kranzusch
Summary: The phase separation of cGAS-DNA is critical for efficient sensing of immunostimulatory DNA, as it limits the activity of the cytosolic exonuclease TREX1. This selective environment suppresses TREX1 function and restricts DNA degradation, balancing cytosolic DNA degradation and innate immune activation.
Editorial Material
Biochemistry & Molecular Biology
Sergio Marques Borghi, Rubia Casagrande, Waldiceu Aparecido Verri
CURRENT MEDICINAL CHEMISTRY
(2023)
Article
Cell Biology
Vanessa de Fatima Borges, Leticia Selinger Galant, Alexandre Kanashiro, Fernanda Vargas e Silva Castanheira, Valter Vinicius Silva Monteiro, Diego angelo Duarte, Filipe Camargo Rodrigues, Camila Meirelles de Souza Silva, Ayda Henriques Schneider, Guilherme Cesar Martelossi Cebinelli, Mikhael Haruo Fernandes de Lima, Joao Paulo de Biaso Viola, Thiago Mattar Cunha, Claudio Miguel da Costa Neto, Jose Carlos Farias Alves-Filho, Andre Sampaio Pupo, Fernando de Queiroz Cunha
Summary: This study investigated the effects of FK506 on sepsis immunopathology and found that FK506 treatment resulted in failed neutrophil migration, leading to increased bacteremia and systemic inflammatory response, causing higher organ damage and mortality rates.
INFLAMMATION RESEARCH
(2023)
Article
Cell Biology
Marcelo Franchin, Thaise Mayumi Taira, Douglas da Silva Prado, Cesar Augusto Speck Hernandez, Fabio Bonifacio de Andrade, Henrique Ballassini Abdalla, Marcelo Henrique Napimoga, Thiago Mattar Cunha, Sandra Yasuyo Fukada, Pedro Luiz Rosalen
Summary: In this study, we investigated the modulatory effects of PI3K gamma on IL-17A expression and the progression of experimental periodontitis in vivo. Our results showed that PI3K gamma inhibitor can attenuate alveolar bone loss and downregulate IL-17A and Rankl gene expression.
INFLAMMATION RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Angela V. F. Alves, Carlisson R. Melo, Jose L. Chagas-Neto, Ricardo G. Amaral, Sergio R. Ambrosio, Monique R. Moreira, Rodrigo C. S. Veneziani, Juliana C. Cardoso, Patricia Severino, Rogerio O. Gondak, Eliana B. Souto, Ricardo L. C. de Albuquerque-Junior
Summary: The antitumor activity of inclusion complexes formed between ent-kaurenoic acid (ERKA) and beta-cyclodextrin (ERKA:beta-CD) was studied in mice, and it was found that ERKA:beta-CD300 showed significant tumor growth inhibition without side effects. This antitumor activity may be attributed to the inhibition of the NF-kB signaling pathway and LDH activity.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Infectious Diseases
Felipe Cerni, Isadora Oliveira, Francielle Cordeiro, Karla Bordon, Isabela Ferreira, Wuelton Monteiro, Eliane Arantes, Thiago Cunha, Manuela Pucca
Summary: This study evaluated the pain behavior induced by T. serrulatus venom and its isolated neurotoxins in mice, and found that different neurotoxins caused varying degrees of pain behavior. Additionally, the researchers developed a mice-derived polyclonal antibody targeting Ts5, which was able to neutralize the effect of this neurotoxin and significantly reduce the nociception produced by the whole venom. This study is the first to explore the effects of different classes of T. serrulatus neurotoxins on nociception, and demonstrates the important role of Ts5 in scorpion sting-induced pain.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Review
Pharmacology & Pharmacy
Sergio M. Borghi, Tiago H. Zaninelli, Jessica B. Carra, Olivia K. Heintz, Marcela M. Baracat, Sandra R. Georgetti, Fabiana T. M. C. Vicentini, Waldiceu A. Verri, Rubia Casagrande
Summary: Asthma is a chronic disease characterized by allergic inflammatory reactions. Current treatment options have limitations and can have adverse effects, leading to poor management of the disease. Flavonoids, with their antioxidant and anti-inflammatory properties, have shown potential benefits for asthma treatment.
Article
Cell Biology
N. B. Amaral, T. S. Rodrigues, M. C. Giannini, M. I. Lopes, L. P. Bonjorno, P. I. S. O. Menezes, S. M. Dib, S. L. G. Gigante, M. N. Benatti, U. C. Rezek, L. L. Emrich-Filho, B. A. Sousa, S. C. L. Almeida, R. Luppino-Assad, F. P. Veras, A. H. Schneider, L. O. S. Leiria, L. D. Cunha, J. C. Alves-Filho, T. M. Cunha, E. Arruda, C. H. Miranda, A. Pazin-Filho, M. Auxiliadora-Martins, M. C. Borges, B. A. L. Fonseca, V. R. Bollela, C. M. Del-Ben, F. Q. Cunha, R. C. Santana, F. C. Vilar, D. S. Zamboni, P. Louzada-Junior, R. D. R. Oliveira
Summary: This study evaluated whether colchicine treatment was associated with the inhibition of NLRP3 inflammasome activation in patients with COVID-19. The results showed that colchicine reduced the need for supplemental oxygen and the length of hospitalization, and lowered the serum levels of Casp1p20 and IL-18 in the first two days of treatment.
INFLAMMATION RESEARCH
(2023)
Article
Immunology
Tamara S. Rodrigues, Camila C. S. Caetano, Keyla S. G. de Sa, Leticia Almeida, Amanda Becerra, Augusto Goncalves, Leticia de Sousa Lopes, Samuel Oliveira, Danielle P. A. Mascarenhas, Sabrina S. Batah, Bruna M. Silva, Giovanni F. Gomes, Ricardo Castro, Ronaldo B. Martins, Jonathan Avila, Fabiani G. Frantz, Thiago M. Cunha, Eurico Arruda, Fernando Q. Cunha, Helder Nakaya, Larissa D. Cunha, Alexandre T. Fabro, Paulo Louzada-Junior, Rene D. R. de Oliveira, Dario S. Zamboni
Summary: SARS-CoV-2 infection activates the NLRP3 inflammasome and leads to inflammation and severe COVID-19. Human caspase-4 (mouse caspase-11) is upregulated and activated by SARS-CoV-2, contributing to NLRP3 activation. In vivo studies using transgenic hACE2 humanized mice show that hACE2 Casp11(-/-) mice are protected from disease development, with reduced lung damage, clinical symptoms, and mortality. Analysis of human samples from fatal COVID-19 cases reveals the expression of CASP4 in patient lungs, which correlates with inflammasome components and inflammatory mediators. These findings suggest CASP4/11 as a potential target for therapeutic interventions in COVID-19.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Immunology
Aryani Felixa Fajardo Martinez, Samuel Cota Teixeira, Guilherme de Souza, Alessandra Monteiro Rosini, Joed Pires de Lima Junior, Gabriel Nogueira Melo, Kelvin Orlando Espinoza Blandon, Angelica Oliveira Gomes, Sergio Ricardo Ambrosio, Rodrigo Cassio Sola Veneziani, Jairo Kenupp Bastos, Carlos Henrique Gomes Martins, Eloisa Amalia Vieira Ferro, Bellisa Freitas Barbosa
Summary: The hydroalcoholic extract and oleoresin from Copaifera multijuga have anti-Toxoplasma gondii activity and do not cause toxicity in human cells. Additionally, they can inhibit adhesion, invasion, and replication of the parasite. These compounds may serve as a new therapeutic strategy for congenital toxoplasmosis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Luana Goncalves Zamarrenho, Mikhael Haruo Fernandes de Lima, Juliana Issa Hori, Jessica Aparecida Lima, Sergio Ricardo Ambrosio, Jairo Kenupp Bastos, David De Jong, Andresa Aparecida Berretta
Summary: We investigated the effects of three different propolis extract formulations and their components on the production of pro- and anti-inflammatory cytokines. The results showed that these propolis extracts had varying effects on the secretion of cytokines.
APPLIED SCIENCES-BASEL
(2023)
Article
Infectious Diseases
Marcela Nunes Argentin, Felipe de Paula Nogueira Cruz, Ariana Borges Souza, Elisa Marcela de Oliveira D'Aurea, Jairo Kenupp Bastos, Sergio Ricardo Ambrosio, Rodrigo Cassio Sola Veneziani, Ilana Lopes Baratella Cunha Camargo, Cassia Suemi Mizuno
Summary: Researchers synthesized PA analogs and tested their antibacterial effects on preformed biofilms of Staphylococcus epidermidis, determining the minimal inhibitory concentration (MIC). The results showed that PA analogs 2a and 3a were able to effectively eradicate preformed biofilms of S. epidermidis and exhibited antibacterial activity against all tested Gram-positive bacteria (Enterococcus faecalis, Enterococcus faecium, S. epidermidis, Staphylococcus aureus) with varying MIC values. Therefore, further in vitro and in vivo testing is warranted to develop antibiotic formulations targeting Gram-positive bacteria.
Article
Chemistry, Medicinal
Mariana B. Santiago, Vinicius Cristian O. dos Santos, Samuel C. Teixeira, Nagela B. S. Silva, Pollyanna F. de Oliveira, Saulo D. Ozelin, Ricardo A. Furtado, Denise C. Tavares, Sergio Ricardo Ambrosio, Rodrigo Cassio S. Veneziani, Eloisa Amalia V. Ferro, Jairo K. Bastos, Carlos Henrique G. Martins
Summary: This study evaluated the potential of Copaifera lucens in combating caries and toxoplasmosis. The results showed that the oleoresin and compound from Copaifera lucens were effective in inhibiting cariogenic bacteria and hindering the intracellular proliferation of Toxoplasma gondii. The study also found that the Copaifera lucens extract exhibited low toxicity. These findings highlight the potential of Copaifera lucens as alternative treatments for caries and toxoplasmosis.
Article
Agriculture, Dairy & Animal Science
Suellen Miguez Gonzalez, Marcela Bortoletto Cerezetti, Larissa Zamparone Bergamo, Camila Rodrigues Ferraz, Waldiceu Aparecido Verri Jr, Marcelo Marcondes Seneda
Summary: The aim of this study was to evaluate oxidative stress in ovaries and corpus luteum (CL) of Bos taurus indicus females and the oxidant effect of CL in ovarian tissues. The results showed that luteal tissues had higher antioxidant activity than ovarian tissues, and there was no difference in antioxidant activity and oxidative stress between ovarian fragments obtained from different regions (with or without CL). The presence of CL in the ovaries did not influence oxidative stress or antioxidant potential.
ANIMAL REPRODUCTION
(2023)
Article
Chemistry, Multidisciplinary
Lucas Braganca Carvalho, Isabela Silva Godoy, Ana Cristina Preisler, Patricia Luiza de Freitas Proenca, Telma Saraiva-Santos, Waldiceu Aparecido Verri Jr, Halley Caixeta Oliveira, Giliardi Dalazen, Leonardo Fernandes Fraceto
Summary: In this study, zein nanoparticles associated with the herbicide atrazine were developed and their efficacy in weed control was evaluated. The nanoformulation showed good stability and encapsulation efficiency. It exhibited greater effectiveness against the target plant, Brassica juncea, even at low doses, without causing toxicity to the crop species. The nanoparticles were captured and accumulated in roots but had limited transportation to the shoot. The atrazine nanoencapsulation did not increase the mobility of the herbicide in the soil.
ENVIRONMENTAL SCIENCE-NANO
(2023)