4.4 Article

Characterizations of coal fly ash nanoparticles and induced in vitro toxicity in cell lines

Journal

JOURNAL OF NANOPARTICLE RESEARCH
Volume 16, Issue 2, Pages -

Publisher

SPRINGER
DOI: 10.1007/s11051-013-2217-x

Keywords

Coal fly ash; Coal fly ash nanoparticles; MTT assay; Cytotoxicity; Environmental and health effects

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The present study illustrates the characterization and cytotoxicity studies of coal fly ash nanoparticles (CFA-NPs). The coal fly ash (CFA) collected from electrostatic precipitator of a coal-fired power plant and the average size of the CFA-NPs was found to be 9-50 nm. Imaging techniques showed predominantly homogenous spherical shaped nanoparticles. The X-ray diffraction analysis and energy dispersive X-ray (EDAX) analysis spectra reveal the elemental constituents of the CFA-NPs contain several toxic heavy metals. Cytotoxicity of CFA-NPs was determined by MTT assay. Cellular metabolism is inhibited in a dose dependent manner by CFA concentrations varying from 13 to 800 mu g mL(-1). After 48 h exposure, the Hep2, A549 and HepG2 cell lines prove more sensitive to CFA-NPs at varying levels which results in IC50 (50 % inhibitory concentration) cytotoxicity end point.

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