Article
Cell Biology
Chad M. Warren, Monika Halas, Paul H. Goldspink, Han-Zhong Feng, Anthony W. Herren, Beata M. Wolska, Pieter P. de Tombe, Jian-Ping Jin, R. John Solaro
Summary: In this study, the impact of chronic expression of cTnI-ND in transgenic mouse hearts was investigated. It was found that the modification of cTnI-ND in the sarcomere initiates changes in the phosphorylation signaling pathways, which may be involved in the chronic-adaptive response to stressors.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2022)
Article
Genetics & Heredity
Linnea C. Wethekam, Jeffrey K. Moore
Summary: In this study, the role of the 5' intron in regulating the expression of alpha-tubulin in Saccharomyces cerevisiae was investigated. The results showed that the intron in alpha-tubulin, TUB1, promotes the expression of alpha-tubulin and cell fitness during microtubule stress. The role of the TUB1 intron depends on its proximity to the TUB1 promoter and distinct sequence features compared to the intron in the alternative alpha-tubulin isotype, TUB3. Several genes involved in chromatin remodeling, alpha/beta-tubulin heterodimer assembly, and the spindle assembly checkpoint were also identified to act with the TUB1 intron.
Article
Cell Biology
Pierre Joanne, Yeranuhi Hovhannisyan, Maximilien Bencze, Marie-Therese Daher, Ara Parlakian, Geraldine Toutirais, Jacqueline Gao-Li, Alain Lilienbaum, Zhenlin Li, Ekaterini Kordeli, Arnaud Ferry, Onnik Agbulut
Summary: Desmin plays a crucial role in muscle remodeling and performance gain induced by mechanical overloading, impacting fatigue resistance and myofibril organization. The lack of desmin affects the functional response to overloading, leading to compromised repair activity of autophagy and altered proteolysis pathways.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biology
Sanford P. Bishop, Jianyi Zhang, Lei Ye
Summary: Death from heart injury is a major cause of mortality worldwide, and the ability of cardiomyocytes to regenerate is limited. This review explores the developmental, structural, and functional changes of cardiomyocytes and their response to myocardial diseases or injuries, providing new insights into the cell cycle regulation of cardiomyocytes.
Article
Biotechnology & Applied Microbiology
M. A. Greene, A. N. S. Udoka, R. R. Powell, R. E. Noorai, T. Bruce, S. K. Duckett
Summary: This study investigated the impact of in utero mycotoxin exposure on skeletal muscle fiber hypertrophy and the miRNA profile in lambs. The results showed that exposure to mycotoxins did not alter fiber type but had long-term effects on postnatal muscle hypertrophy and cross-sectional area. Additionally, developmental age influenced the miRNA transcriptome and mRNA expression of genes related to muscle growth.
Article
Cell Biology
Kuan-Ho Lin, V. Bharath Kumar, Tamilselvi Shanmugam, Marthandam Asokan Shibu, Ray-Jade Chen, Chia-Hua Kuo, Tsung-Jung Ho, V. Vijaya Padma, Yu-Lan Yeh, Chih-Yang Huang
Summary: miR-145-5p inhibits cardiac hypertrophy by targeting paxillin and modulating Rac-1/JNK/c-jun/NFATc3/ANP/Sirt1 signaling pathways, revealing a novel downstream molecular mechanism in the development of cardiac hypertrophy.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Giovanna Priante, Monica Ceol, Lisa Gianesello, Dario Bizzotto, Paola Braghetta, Lorenzo Arcangelo Calo, Dorella Del Prete, Franca Anglani
Summary: Dent disease (DD1) is a rare tubulopathy caused by mutations in the CLCN5 gene. Our study revealed down-regulation of ClC-5 and nephrin expression in podocytes of DD1 patients, indicating a potential link between ClC-5 loss and glomerular damage. In vitro experiments further confirmed that mutant clones with CLCN5 mutations showed significantly decreased expression of ClC-5 and nephrin, along with abnormal cytoskeleton.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Sarah-Eve Lemay, Charifa Awada, Tsukasa Shimauchi, Wen-Hui Wu, Sebastien Bonnet, Steeve Provencher, Olivier Boucherat
Summary: Pulmonary arterial hypertension is a debilitating chronic disorder that puts pressure overload on the heart, leading to structural and functional changes, ultimately causing heart failure and death. Recent research suggests that overactivation of fetal genes may play a critical role in the process of pulmonary arterial hypertension, exploring whether this discovery is a causative factor or a protective mechanism.
Article
Agriculture, Dairy & Animal Science
Fatemeh Mohammadinejad, Mohammadreza Mohammadabadi, Zahra Roudbari, Tomasz Sadkowski
Summary: This study aimed to identify the hub genes and mechanisms involved in skeletal muscle maturation and hypertrophy in livestock species (Bos taurus, Ovis aries, and Sus scrofa). Gene expression profiles were analyzed, and differentially expressed genes and enriched pathways were identified in each species. Common genes and pathways were also identified, which play important roles in the development and maturation of skeletal muscles. This study provides valuable insights into the relationships between genes and biological pathways in the skeletal muscle maturation process.
Article
Oncology
Wen-Sheng Wu, Chuan-Chu Cheng, Yi-Hsuan Lee, Jia-Ling Wei, Rui-Fang Chen, Chen -Fang Lin, Ren-In You, Yen -Chang Chen, Hsiu-Ming Shih, Chi -Tan Hu, Hsin-Hou Chang, Ming-Che Lee, Yen-Cheng Chen
Summary: Hic-5 is an important signaling mediator in HCC, and targeting Hic-5 may prevent the progression of HCC by regulating Snail and its downstream genes involved in migration and invasion. In vivo experiments showed that knock down of Hic-5 prevented the progression of HCC and decreased the expression of downstream mesenchymal genes, providing preclinical evidence for the potential of targeting Hic-5.
AMERICAN JOURNAL OF CANCER RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Jelena Nikolic, Katarina Vukojevic, Violeta Soljic, Josip Miskovic, Martina Orlovic Vlaho, Mirna Saraga-Babic, Natalija Filipovic
Summary: We studied the expression of serotonin receptors 5-HT1A, 5-HT2A, and 5-HT3A at different stages of fetal lung development. The receptors were strongly expressed in the epithelium of the proximal airways and vascular walls during early and mid-fetal development. In the later stages, they were also expressed in type I and type II pneumocytes. The expression of 5-HT2A decreased while 5-HT3A expression increased toward the final stage of lung development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Ajit Magadum, Neha Singh, Ann Anu Kurian, Mohammad Tofael Kabir Sharkar, Nishat Sultana, Elena Chepurko, Keerat Kaur, Magdalena M. Zak, Yoav Hadas, Djamel Lebeche, Susmita Sahoo, Roger Hajjar, Lior Zangi
Summary: Heart failure remains a major issue globally, with cardiac hypertrophy and fibrosis being key factors. The regulator Pip4k2c, associated with mTORC1, plays a role in these processes. Studies show that deleting Pip4k2c does not affect embryonic cardiac development, but leads to increased rates of CH, CF, and sudden death in adult mice. Upregulating Pip4k2c improves heart function, reverses CH and CF, and enhances survival through inhibiting TGF beta 1 via specific pathways. Loss-and-gain-of-function studies identify Pip4k2c as a potential therapeutic target for CF, CH, and HF, utilizing modRNA as an effective gene therapy approach.
Article
Cell Biology
Samantha M. Richter, Lilyanna C. Massman, Dennis J. Stuehr, Elizabeth A. Sweeny
Summary: NADPH oxidase 5 (NOX5) is a transmembrane oxidative signaling enzyme that produces superoxide in response to intracellular calcium flux. It is involved in physiological processes and human disease, but its signaling pathways and targets of oxidative modifications remain poorly understood. This study demonstrates that NOX5 and actin interact in the cell and modulate each other's activity. Changes in actin dynamics affect NOX5 superoxide production, and NOX5 oxidatively modifies actin. Knockdown of NOX5 impairs cell migration. These findings reveal an important link between actin dynamics and oxidative signaling through NOX5.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Clinical Neurology
Payam Mohassel, Pomi Yun, Safoora Syeda, Abhinandan Batra, Andrew J. Bradley, Sandra Donkervoort, Soledad Monges, Julie S. Cohen, Doris G. Leung, Francina Munell, Carlos Ortez, Angel Sanchez-Montanez, Peter Karachunski, John Brandsema, Livija Medne, Vinay Chaudhry, Giorgio Tasca, A. Reghan Foley, Bjarne Udd, Andrew E. Arai, Glenn A. Walter, Carsten G. Bonnemann
Summary: In this study, we examined FHL1-related reducing body myopathy patients using muscle ultrasound, muscle MRI, and cardiac MRI. The results showed that muscle ultrasound and muscle MRI are practical and informative tools for diagnosis and monitoring of disease progression.
ANNALS OF CLINICAL AND TRANSLATIONAL NEUROLOGY
(2023)
Article
Obstetrics & Gynecology
Matthew A. Shanahan, Kjersti M. Aagaard, Laurence B. McCullough, Francis A. Chervenak, Alireza A. Shamshirsaz
Summary: Despite obstacles such as incomplete understanding of the fetal immune system, maternal immune responses to in utero gene therapy, risks of off-target effects from gene editing, gestational age constraints, and ethical questions surrounding fetal genetic intervention, none of these barriers appears insurmountable, and the journey to in utero gene therapy for sickle cell disease and other conditions should be well underway.
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Andrew B. Harvey, Renelyn A. Woltes, Raymond N. Deepe, Hannah G. Tarolli, Jenna R. Drummond, Allison Trouten, Auva Zandi, Jeremy L. Barth, Rupak Mukherjee, Martin J. Romeo, Silvia G. Vaena, Ge Tao, Robin Muise-Helmericks, Paula S. Ramos, Russell A. Norris, Andy Wessels
Summary: This study highlights the importance of SOX9 in the regulation of epicardial cell invasion and emphasizes the role of EPDCs in regulating atrioventricular valve development and homeostasis. It also reports a novel expression profile of Cd109, a gene with previously unknown relevance in heart development.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2024)
Review
Cardiac & Cardiovascular Systems
MariaSanta C. Mangione, Jinhua Wen, Dian J. Cao
Summary: mTOR, a mechanistic target of rapamycin, is an evolutionarily conserved pathway that plays a fundamental role in nutrient sensing, growth, metabolism, lifespan, and aging. Recent studies have highlighted the regulatory role of mTOR in innate immune responses and its involvement in the pathogenesis of cardiovascular diseases, especially in acute inflammation and atherosclerotic cardiovascular disease. This review also discusses mTOR's role in trained immunity, immune senescence, and clonal hematopoiesis, as well as its architecture and regulatory complexes.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2024)
Article
Cardiac & Cardiovascular Systems
Junlin Li, Yajun Gong, Yiren Wang, Huihui Huang, Huan Du, Lianying Cheng, Cui Ma, Yongxiang Cai, Hukui Han, Jianhong Tao, Gang Li, Panke Cheng
Summary: Myocardial ischemia-reperfusion injury is closely related to the final infarct size in acute myocardial infarction. Regulatory T cells play an important role in the inflammatory response after AMI, but different subtypes of Tregs have different effects on the injury.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2024)
Article
Cardiac & Cardiovascular Systems
Yuxin Chu, Yutao Hua, Lihao He, Jin He, Yunxi Chen, Jing Yang, Ismail Mahmoud, Fanfang Zeng, Xiaochang Zeng, Gloria A. Benavides, Victor M. Darley-Usmar, Martin E. Young, Scott W. Ballinger, Sumanth D. Prabhu, Cheng Zhang, Min Xie
Summary: This study demonstrates that administering beta-hydroxybutyrate (beta-OHB) at the time of reperfusion can reduce infarct size and preserve cardiac function by activating autophagy and preserving mitochondrial homeostasis, potentially through mTOR inhibition.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2024)