Article
Biotechnology & Applied Microbiology
Hongyi Liu, Shaoqing Wang, Bo Lang, Yaqian Li, Xinhua Wang, Jie Chen
Summary: In this study, it was found that the engineered Trichoderma afroharzianum strain OE:SCf, which expresses the fused Sm1-Chit42 protein, exhibited synergistic inhibition against Botrytis cinerea growth and reproduction. The SCf protein showed inhibitory effects at multiple stages of the mycoparasitic interaction between T. afroharzianum and B. cinerea, including chemotropism sensing, hyphal coiling, hydrophobicity modulation, cell wall adhesion, virulence reduction, and pathogen killing by ROS. These findings highlight a novel mycoparasitic system in Trichoderma strains engineered with chimeric proteins to combat B. cinerea, providing a solid foundation for future development of engineered Trichoderma biofungicides created with chimeric proteins.
MICROBIAL CELL FACTORIES
(2023)
Article
Biotechnology & Applied Microbiology
Lijiahong Geng, Yalun Fu, Xiaohua Peng, Zhengkun Yang, Mengke Zhang, Zhiyu Song, Ning Guo, Shuangchen Chen, Junliang Chen, Bing Bai, Airong Liu, Golam Jalal Ahammed
Summary: This study investigated the effects of Trichoderma harzianum on Botrytis cinerea and its underlying mechanisms. The results showed that Trichoderma harzianum enhanced tomato resistance to gray mold disease by regulating cellular redox, secondary metabolites, and stomatal movement.
BIOLOGICAL CONTROL
(2022)
Article
Multidisciplinary Sciences
G. Ramangouda, M. K. Naik, Rahul Nitnavare, Richa Yeshvekar, Joorie K. Bhattacharya, Pooja K. Bhatnagar-Mathur, Mamta K. Sharma
Summary: This study investigated the biocontrol activities and carbendazim tolerance of N-methyl-n-nitro-N-nitrosoguanidine (NTG) induced mutants of Trichoderma asperellum. Six NTG induced mutants were developed, with N2-3, N2-1, N1, and N2-2 showing the best antagonistic activities against fungi and volatile metabolite activities. Mutant N2-2 exhibited the highest carbendazim tolerance and was able to sporulate even at high concentrations of carbendazim. These mutants were found to have mutations in the tub2 gene, but the enhanced carbendazim tolerance did not affect their mycoparasitism and plant growth activity. Overall, the mutants showed similar attributes to the wild-type.
Article
Agronomy
Seiya Saito, Fei Wang, Chang-Lin Xiao
Summary: This study evaluated the efficacy of natamycin as an alternative fungicide for controlling gray mold of blueberries. The results showed that natamycin significantly reduced gray mold incidence and lesion size, and decreased overall fruit decay caused by natural infections, without adverse effects on fruit quality.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Xiaodi Niu, Ziyou Wang, Chenyang Wang, Hongsu Wang
Summary: Chitinase is an important component in fungal growth and reproduction, and a small molecule called DBA has been discovered to inhibit Botrytis cinerea Chitinase. This study has shown that DBA can extend the shelf life of cherry tomatoes, making it a promising antifungal agent for fruit preservation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Negin Eslahi, Mojegan Kowsari, Mohammad Reza Zamani, Mostafa Motallebi
Summary: The study showed that recombinant strains T15 and T13 of Trichoderma harzianum have stronger inhibitory effects against Rhizoctonia solani, leading to increased defense enzymes activity and enhanced expression of defense-related genes in bean plants. Additionally, analysis of volatile organic compounds (VOCs) revealed the production of compounds known to induce plant defensive reactions against stress by the recombinant strains. Overall, inoculation with these recombinant strains resulted in better protection mechanisms and colonization within the soil and root surface, enhancing defense mechanisms against R. solani.
BIOLOGICAL CONTROL
(2021)
Article
Biochemistry & Molecular Biology
Consuelo Olivares-Yanez, Evelyn Sanchez, Gabriel Perez-Lara, Aldo Seguel, Pamela Y. Camejo, Luis F. Larrondo, Elena A. Vidal, Paulo Canessa
Summary: By generating manually curated transcription factor datasets and conducting network analysis, we studied the transcriptional gene regulatory networks of Botrytis and Trichoderma, revealing significant differences in their transcriptomes under different biological paradigms and distinctive gene expression changes due to confrontation.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Multidisciplinary Sciences
Hind Lahmyed, Rachid Bouharroud, Redouan Qessaoui, Abdelhadi Ajerrar, Abderrahim Amarraque, Moulay Abdelazize Aboulhassan, Bouchra Chebli
Summary: This study isolated actinomycete bacteria with potential antagonistic abilities towards Botrytis cinerea from the rhizosphere of a healthy tomato grove. Some strains exhibited good antagonistic activity and plant growth-promoting characteristics. These results suggest that these actinomycetes may offer a potential biological control method against gray mold caused by B. cinerea on tomato fruits.
KUWAIT JOURNAL OF SCIENCE
(2021)
Article
Microbiology
Yaping Wang, Xiaochong Zhu, Jian Wang, Chao Shen, Wei Wang
Summary: In this study, transcriptomic sequencing and real-time fluorescence quantitative PCR (RT-qPCR) were used to investigate the mycoparasitism mechanism of Trichoderma harzianum T4. The results showed that certain genes associated with fungal antagonism were significantly upregulated at specific growth time points, confirming their involvement in the mycoparasitism ability of T4. These findings not only improve the annotation information of gene models in T4 genome, but also provide important transcriptome information regarding the process of mycoparasitism, thereby offering a potential molecular target for T4 as a biological control agent.
Article
Biochemistry & Molecular Biology
Kou-Cheng Peng, Chao-Chi Lin, Chong-Fu Liao, Hsin-Chiao Yu, Chaur-Tsuen Lo, Hsueh-Hui Yang, Kuo-Chih Lin
Summary: The study demonstrates that ThLAAO can activate defense mechanisms in transgenic tobacco, enhancing resistance against Sclerotinia sclerotiorum and Botrytis cinerea, and function through H2O2 signaling in chloroplasts.
Article
Biology
Marlene Henriquez-Urrutia, Rebecca Spanner, Consuelo Olivares-Yanez, Aldo Seguel-Avello, Rodrigo Perez-Lara, Hector Guillen-Alonso, Robert Winkler, Alfredo Herrera-Estrella, Paulo Canessa, Luis F. Larrondo
Summary: This study confirms the existence of circadian rhythms in Trichoderma atroviride and highlights the importance of these rhythms in the fungal-fungal interaction with Botrytis cinerea. The study also reveals the impact of clock components on various aspects of T. atroviride's lifestyle and the modulation of fungal interactions and dynamics.
Article
Environmental Sciences
Anirvan Mukherjee, Swapan Kumar Ghosh
Summary: This study found that Trichoderma harzianum is more effective than Beauveria bassiana in controlling aphids, and it also has a better enzyme production capacity. This provides a method for the eco-friendly biocontrol of aphids by replacing harmful chemical pesticides.
ENVIRONMENTAL MONITORING AND ASSESSMENT
(2023)
Article
Microbiology
Fayaz A. Mohiddin, Shahid A. Padder, Arif H. Bhat, Mohammad A. Ahanger, Asif B. Shikari, Shabir H. Wani, Farooq A. Bhat, Sajad Un Nabi, Aflaq Hamid, Nazir A. Bhat, Najeebul R. Sofi, Showkat A. Waza, Burhan Hamid, Shugufta Parveen, Ashaq Hussain, Aadil N. Bhat, Omar M. Ali, Mohammad S. Dar, Arafat Abdel Hamed Abdel Latef
Summary: Trichoderma harzianum, with varying chitinase activity levels, showed inhibitory effects on different pathogens both in vitro and in vivo conditions. The presence of certain metal ions enhanced chitinase production while others caused down-regulation. Isolate BT4 exhibited the best disease control on Fusarium oxysporum with 48% efficacy. The findings suggest the potential use of identified Trichoderma harzianum in disease management under field conditions.
Article
Agronomy
Muhammad Imran, Kamal A. M. Abo-Elyousr, Mohamed E. El-Sharnouby, Esmat F. Ali, Nashwa M. A. Sallam, Hadeel M. M. Khalil Bagy, Ismail R. Abdel-Rahim
Summary: The Trichoderma harzianum isolate Tr-3 and zinc nanoparticles (ZnO-NPs) were found to have significant biocontrol efficacy against Botrytis cinerea, making them potential alternatives to fungicides for controlling gray mold disease in tomatoes.
Article
Entomology
Massinissa Hammad, Thomas Guillemette, Meriem Alem, Franck Bastide, Meriem Louanchi
Summary: This study identified fifteen isolates of Trichoderma spp. from different plants and compost in Algeria. These isolates exhibited high antagonistic activity against Botrytis cinerea, with one isolate showing a 90.68% inhibition of germling growth in Culture filtrates test. Biocontrol tests on tomato plants with selected Trichoderma isolates also showed a significant reduction in disease incidence. The study highlights the potential use of native strains of Trichoderma for biological control to preserve soil microbial diversity.
EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL
(2021)
Article
Multidisciplinary Sciences
Qiong Wu, Ruiyan Sun, Mi Ni, Jia Yu, Yaqian Li, Chuanjin Yu, Kai Dou, Jianhong Ren, Jie Chen
Article
Multidisciplinary Sciences
Qiong Wu, Lida Zhang, Hai Xia, Chuanjin Yu, Kai Dou, Yaqian Li, Jie Chen
SCIENTIFIC REPORTS
(2017)
Article
Biotechnology & Applied Microbiology
Qiong Wu, Mi Ni, Guisheng Wang, Qianqian Liu, Meixia Yu, Jun Tang
Article
Environmental Sciences
Mi Ni, Qiong Wu, Junli Wang, Wei C. Liu, Jian H. Ren, Dian P. Zhang, Juan Zhao, D. E. W. Liu, Ying H. Rao, Cai G. Lu
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES
(2018)
Article
Biochemistry & Molecular Biology
Qiong Wu, Caige Lu, Mi Ni, Hongli Wang, Weicheng Liu, Jie Chen
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Qiong Wu, Yaqian Li, Yingying Li, Shigang Gao, Meng Wang, Tailong Zhang, Jie Chen
Article
Biochemistry & Molecular Biology
Mi Ni, Qiong Wu, Hong-li Wang, Wei-cheng Liu, Bin Hu, Dian-peng Zhang, Juan Zhao, De-wen Liu, Cai-ge Lu
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
(2019)
Article
Biotechnology & Applied Microbiology
Qiong Wu, Mi Ni, Kai Dou, Jun Tang, Jianhong Ren, Chuanjin Yu, Jie Chen
MICROBIAL CELL FACTORIES
(2018)
Article
Environmental Sciences
Mi Ni, Qiong Wu, Gui S. Wang, Qian Q. Liu, Mei X. Yu, Jun Tang
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES
(2019)
Article
Plant Sciences
Q. Wu, M. M. Hou, L. Y. Li, L. J. Liu, Y. X. Hou, G. Z. Liu
JOURNAL OF PLANT PATHOLOGY
(2011)