Article
Veterinary Sciences
Rasaq A. Ojasanya, Ian A. Gardner, David Groman, Sonja Saksida, Matthew E. Saab, Krishna K. Thakur
Summary: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) allows rapid and reliable identification of microorganisms. This study reports the development and validation of a novel MALDI-TOF MS main spectral profile (MSP) for rapid identification of Yersinia ruckeri isolated from Atlantic salmon. The novel MSP showed high analytical sensitivity and specificity.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Review
Microbiology
Fernando Sanchez-Juanes, Noelia Calvo Sanchez, Moncef Belhassen Garcia, Carmen Vieira Lista, Raul Manzano Roman, Rufino Alamo Sanz, Antonio Muro Alvarez, Juan Luis Munoz Bellido
Summary: Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a simple, fast, and specific method used for bacterial identification, and has also been shown to be useful for identifying certain parasites and arthropod vectors. Fast, simple, and reliable methods for identifying arthropod vectors are crucial for controlling diseases transmitted by these vectors.
Review
Environmental Sciences
Mohammad Y. Ashfaq, Dana A. Da'na, Mohammad A. Al-Ghouti
Summary: Bacteria play diverse roles in the environment, with MALDI-TOF MS technology gaining attention for routine bacterial identification. This technique has been applied in various environmental studies, showing benefits in rapid and cost-effective bacteria identification.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Article
Parasitology
Roland Bamou, Monique Melo Costa, Adama Zan Diarra, Ademir Jesus Martins, Philippe Parola, Lionel Almeras
Summary: This study assessed the optimal conditions for mosquito identification using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling. Three different homogenization methods were used on three distinct body parts of two mosquito species, and the results were evaluated. The thorax and legs were found to be the most suitable specimens for identification, regardless of species or homogenization method. The automatic homogenization procedure was selected as the reference method due to its adaptability to processing a large number of samples. Blood-feeding status did not affect species identification, but blood peaks were present in the mass spectra of all body parts tested. Adding species spectra from the same geographical area improved identification scores for field-collected specimens.
PARASITES & VECTORS
(2022)
Article
Parasitology
Ly Na Huynh, Adama Zan Diarra, Hong Sang Nguyen, Long Bien Tran, Van Nguyen Do, Tran Duc Anh Ly, Van Hoang Ho, Xuan Quang Nguyen, Philippe Parola
Summary: This study utilized MALDI-TOF MS to successfully identify various mosquito species from Vietnam, adding to the reference spectral database.
PARASITES & VECTORS
(2022)
Article
Biochemistry & Molecular Biology
Chia-Ru Chung, Hsin-Yao Wang, Po-Han Chou, Li-Ching Wu, Jang-Jih Lu, Jorng-Tzong Horng, Tzong-Yi Lee
Summary: This study used an ensemble of multiple preprocessing methods to extract critical information from complicated mass spectrometry spectral data for identifying microorganisms and predicting antibiotic resistance. The ensemble method outperformed individual methods, achieving the highest accuracy on independent testing datasets. Important peaks related to antibiotic resistance could be detected, providing valuable information for further investigation of the resistance mechanism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Microbiology
Praveen Rahi, Estelle Muhle, Cyril Scandola, Gerald Touak, Dominique Clermont
Summary: This study utilized a polyphasic taxonomy approach to accurately classify six strains within the Enterobacter cloacae complex (ECC). The 16S rRNA gene-based phylogeny failed to group all ECC species into a monophyletic cluster, while genome sequence-based phylogenetic approaches successfully grouped all species. A new species named Enterobacter pasteurii sp. nov. was proposed based on comprehensive genotypic and phenotypic characterizations.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biotechnology & Applied Microbiology
M. Moussa, E. Cauvin, A. Le Piouffle, O. Lucas, A. Bidault, C. Paillard, F. Benoit, B. Thuillier, M. Treilles, M. A. Travers, Celine Garcia
Summary: This study developed a MALDI-TOF MS method for rapid identification of a variety of Vibrio species in mollusk aquaculture, creating the EnviBase library with 120 main spectra projections. The EnviBase library was validated through a blind test on 100 Vibrio strains, successfully identifying the majority of the strains.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Gyu Ri Kim, Si Hyun Kim, Eun-Young Kim, Eun Hee Park, In Yeong Hwang, Seok Hoon Jeong, Hyun Soo Kim, Young Ah Kim, Young Uh, Kyeong Seob Shin, Young Ree Kim, Namhee Ryoo, Jong Hee Shin, Jeong Hwan Shin
Summary: This study evaluated the usefulness of MALDI-TOF VITEK MS for Salmonella identification. The results showed that VITEK MS could accurately identify most Salmonella species, but had lower accuracy in identifying typhoidal Salmonella and Salmonella enterica ssp. diarizonae isolates.
Article
Biochemistry & Molecular Biology
Xia Wang, Guanqi Wu, Shuo Han, Jingjing Yang, Xiangwei He, Haifang Li
Summary: Plant endophytic bacteria play important roles in plant growth and stress resistance. This study identified and classified endophytic bacteria in Populus based on MALDI-TOF MS data and 16S rRNA gene sequencing, and verified the classification capacity of protein mass fingerprints. The results provide a valuable reference for differentiating and identifying endophytic bacteria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Claudia Honsig, Brigitte Selitsch, Marlene Hollenstein, Matthias G. Vossen, Kathrin Spettel, Birgit Willinger
Summary: In this study, the identification of 230 filamentous fungi isolates using MALDI-TOF MS in combination with the Filamentous Fungi Library 3.0 was investigated. The results showed that using the more time-consuming sample preparation methods improved species-level identification rates, and the in-house threshold for species identification was validated for routine use in the laboratory. This suggests that this commercial platform is suitable for routine diagnosis.
Article
Chemistry, Applied
Qiuxia Yang, Yushan Guo, Yueming Jiang, Bao Yang
Summary: Oligosaccharides are recognized as prebiotics and their structure is closely related to bioactivity. In this study, UPLC-MS/MS was developed to identify the structure of oligosaccharides, which mainly appeared as sodium adduct in the chromatogram. The fragmentation patterns of oligosaccharides were summarized for different glycosidic linkages. The results showed that UPLC-MS/MS was effective in identifying the structure of oligosaccharides and could be applied in polysaccharide structure identification.
FOOD HYDROCOLLOIDS
(2023)
Article
Chemistry, Applied
Xiaojing Sheng, Satoshi Nagaoka, Marika Hashimoto, Yusuke Amiya, Masato Beppu, Kenichiro Tsukamoto, Emiko Yanase, Mitsuru Tanaka, Toshiro Matsui
Summary: Beta-conglycinin was orally administered to rats, and specific oligopeptides derived from the protein were identified in the blood plasma, indicating that beta-conglycinin could be the dietary source protein for these peptides found in the circulating bloodstream of rats.
Review
Chemistry, Multidisciplinary
Muhammad Ramzan, Ali Raza, Zaib Un Nisa, Syed Ghulam Musharraf
Summary: The continuous development of resistance to antibiotic drugs by microorganisms causes high mortality and morbidity. Pathogens with distinct features and biochemical abilities make them destructive to human health. Therefore, early identification of the pathogen is of substantial importance for quick ailments and healthcare outcomes. Several advanced spectroscopic techniques have been utilized for the identification of microorganisms, particularly bacteria, in research studies conducted in the last five years. Among these techniques, mass spectrometry (MS), infrared (IR) spectroscopy, Raman spectroscopy (RS), and nuclear magnetic resonance (NMR) spectroscopy have been widely used. MALDI-TOF/MS, a type of MS technique, has been particularly favored for microbial identification. The review discusses the identification of 44 bacteria using these techniques, with the most studied organisms being E. coli, S. aureus, and other microorganisms.
ARABIAN JOURNAL OF CHEMISTRY
(2023)
Article
Biochemical Research Methods
Saidou Ahamada M'madi, Adama Zan Diarra, Lionel Almeras, Philippe Parola
Summary: The objective of this study is to find the optimal conditions for preparing tick samples with quality and reproducible MS spectra that can be stored for more than ten years. Among the four protocols tested, the ReDO and PReDO protocols show good results for the MALDI-TOF MS identification of ticks from old collections preserved in alcohol or dry. MALDI-TOF MS is a promising tool for identifying ticks, including vectors responsible for the transmission of diseases.
JOURNAL OF PROTEOMICS
(2022)
Article
Food Science & Technology
K. Torma, J. Lunden, E. Kaukonen, M. Fredriksson-Ahomaa, R. Laukkanen-Ninios
Summary: Meat inspection of broiler chickens in the European Union is regulated by common legislation for meat safety, but challenges arise in conducting proper post-mortem inspection in slaughterhouses due to varying inspection times per broiler and deficiencies in visual inspection and facilities. More precise guidelines and recommendations are needed, especially for inspection time and lighting at inspection stations.
Article
Biotechnology & Applied Microbiology
Etinosa Osemwowa, Iyekhoetin Matthew Omoruyi, Paula Kurittu, Annamari Heikinheimo, Maria Fredriksson-Ahomaa
Summary: The study reveals a significant difference in bacterial contamination levels on raw beef surfaces between Nigeria and Finland, with Nigerian samples showing higher bacterial counts than Finnish samples, and the majority of Nigerian samples being unacceptable according to EU standards. Furthermore, beef samples from Nigeria have higher contamination rates of Salmonella, STEC, and CREC compared to samples from Finland.
Article
Microbiology
Kaisa Kuus, Toomas Kramarenko, Jelena Sogel, Mihkel Maeesaar, Maria Fredriksson-Ahomaa, Mati Roasto
Summary: This study provides an overview of the prevalence and serotype diversity of Salmonella in the meat production chain in Estonia over a 5-year period, with corresponding data on human salmonellosis. It was found that monophasic S. Typhimurium has become increasingly important in Estonia in recent years, ranking second in human cases and third among the most common serotypes of Salmonella enterica.
Correction
Biodiversity Conservation
Maria Fredriksson-Ahomaa, Laura London, Teresa Skrzypczak, Tuija Kantala, Ilona Laamanen, Mia Bistrom, Leena Maunula, Tuija Gadd
Article
Microbiology
Laura Blomvall, Kirsti Pelkola, Taru Lienemann, Suvi Lehtoniemi, Leena Pohjola, Maria Fredriksson-Ahomaa
Summary: A Y. pseudotuberculosis outbreak was diagnosed in a male turkey flock in Finland, causing osteomyelitis and lameness. Whole-genome sequencing revealed that all isolates belonged to the same strain, indicating a common source of infection. High rates of carcass condemnation were observed in the slaughterhouse, highlighting the importance of food chain information.
VETERINARY MICROBIOLOGY
(2022)
Article
Microbiology
Juho Koskinen, Pilar Ortiz-Martinez, Riikka Keto-Timonen, Suvi Joutsen, Maria Fredriksson-Ahomaa, Hannu Korkeala
Summary: Yersinia enterocolitica is a psychrotrophic zoonotic foodborne pathogen mainly found in pigs. The antimicrobial resistance of Y. enterocolitica 4/O:3 strains varies significantly between countries, with higher resistance observed in Spain and Italy. Generally, countries with higher sales of antimicrobials for food production animals and common oral administration have higher levels of antimicrobial resistance.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Nevijo Zdolec, Marta Kis, Dean Jankuloski, Katerina Blagoevska, Snjezana Kazazic, Marina Pavlak, Bojan Blagojevic, Dragan Antic, Maria Fredriksson-Ahomaa, Valerij Pazin
Summary: This study investigated the prevalence and antimicrobial resistance of Yersinia enterocolitica in pigs from different housing systems. The results showed variations in both the quantity and prevalence of the pathogen. Pigs from family farms were more susceptible to Yersinia enterocolitica, while antimicrobial-resistant strains were found in pigs from large integrated farms. However, no pathogenic Yersinia enterocolitica was detected in pork samples.
Article
Food Science & Technology
K. Torma, E. Kaukonen, J. Lunden, M. Fredriksson-Ahomaa, R. Laukkanen-Ninios
Summary: Comprehensive, reliable, and comparable meat inspection (MI) data are essential for monitoring and regulating the health and welfare of broiler chickens. However, there are differences and incomparability between different slaughterhouses regarding MI criteria and condemnation causes. Only when the differences and reliability of the data are known can they be used for monitoring and surveillance purposes. Harmonizing the condemnations of carcasses with diseases that most threaten broiler health and welfare and cause the largest economic losses is important.
Article
Food Science & Technology
Mikaela Sauvala, Per Johansson, Johanna Bjorkroth, Maria Fredriksson-Ahomaa
Summary: Vacuum packaging is widely used to extend the shelf life of fresh meat products, but there is little information on its effects on deer meat. This study evaluated the microbial quality and safety of vacuum-packaged deer meat cuts stored at 4 degrees C. The results showed a significant decrease in odor and appearance scores, and an increase in mesophilic aerobic bacteria (MAB) and lactic acid bacteria (LAB) counts after 3 weeks of storage. Spoilage changes were observed after 3 weeks, and the dominant bacteria genus identified was Lactobacillus.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Food Science & Technology
Maria Fredriksson-Ahomaa, Mikaela Sauvala, Paula Kurittu, Viivi Heljanko, Annamari Heikinheimo, Peter Paulsen
Summary: The study found that potentially virulent and antimicrobial-sensitive L. monocytogenes isolates associated with human listeriosis are commonly found in hunted game and game meat in Finland.
Article
Microbiology
Triin Tedersoo, Mati Roasto, Mihkel Maesaar, Maria Fredriksson-Ahomaa, Kadrin Meremae
Summary: Pigs are the main reservoirs of Campylobacter coli, a common cause of gastrointestinal disease in humans. Little is known about the role of pork in transmitting these bacteria. This study found that a high proportion of Campylobacter isolates from caecal samples of fattening pigs in Estonia were resistant to several antimicrobials, indicating that the pork production chain is an important source of antimicrobial-resistant C. coli.
Article
Food Science & Technology
Laura Blomvall, Eija Kaukonen, Paula Kurittu, Annamari Heikinheimo, Maria Fredriksson-Ahomaa
Summary: This study investigated the food chain information (FCI) and post-mortem inspection (PMI) results of Finnish slaughter turkeys, and tested for various microbial public health hazards. The FCI showed deviations in 44% of the sampled turkey flocks, and overall, 68% of the flocks carried microbial public health hazards.
Article
Food Science & Technology
Francesca Piras, Giuliana Siddi, Anne-Sophie Le Guern, Sylvie Bremont, Maria Fredriksson-Ahomaa, Rita Sanna, Maria Pina Meloni, Enrico Pietro Luigi De Santis, Christian Scarano
Summary: Between 2018 and 2019, a study was conducted in two industrial cheese-making plants in Sardinia to investigate the presence of Y. enterocolitica and characterize the isolates. Y. enterocolitica was detected in 7.4% of the samples, with a higher prevalence in non-food contact surface samples (10.2%) compared to food contact surface samples (3.8%). The isolates showed genetic diversity and potential pathogenicity. Contamination of cheese-making plants with Y. enterocolitica can pose a risk to human health. Preventive measures such as hygienic design and proper cleaning programs are important.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Veterinary Sciences
Rauni Kivisto, Mikaela Sauvala, Maria Fredriksson-Ahomaa, Johanna Bjorkroth
Summary: C. jejuni is commonly found in reared pheasants, and different genotypes were identified in this study. The ST-45 genotype was closely related to human clinical isolates, while the dominant ST-699 genotype was distinct from other sources worldwide. Hygienic measures and improved biosecurity are necessary to control the spread of infection and reduce the zoonosis risk associated with pheasant farming.
ACTA VETERINARIA SCANDINAVICA
(2023)
Article
Food Science & Technology
Abbey Olsen, Silvia Bonardi, Lisa Barco, Marianne Sandberg, Nina Langkabel, Mati Roasto, Michal Majewski, Brigitte Brugger, Arja H. Kautto, Bojan Blagojevic, Joao B. Cota, Gunvor Elise Nagel-Alne, Adeline Huneau, Riikka Laukkanen-Ninios, Sophie Lebouquin-Leneveu, Ole Alvseike, Maria Fredriksson-Ahomaa, Madalena Vieira-Pinto, Eija Kaukonen
Summary: Campylobacter is a significant foodborne pathogen in Europe, mainly transmitted through undercooked poultry meat. Many European countries have implemented measures to reduce human exposure to Campylobacter from broiler meat. National action plans have been effective in reducing the incidence of human campylobacteriosis, as demonstrated by Iceland.