Article
Plant Sciences
Ramaraj Sathasivam, Minsol Choi, Ramalingam Radhakrishnan, Haejin Kwon, Jiwon Yoon, So Hwi Yang, Jae Kwang Kim, Yong Suk Chung, Sang Un Park
Summary: The hairy root (HR) culture system is an excellent alternative strategy for mass production of valuable compounds. Selection of suitable Agrobacterium strain is essential for enhanced production of beneficial secondary metabolites. In this study, Agrobacterium strain R1601 was found to be one of the most promising strains for inducing mass HR production and enhanced levels of secondary metabolites in Ocimum basilicum.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Diksha Bagal, Aksar Ali Chowdhary, Shakti Mehrotra, Sonal Mishra, Sonica Rathore, Vikas Srivastava
Summary: Plants are important sources of secondary metabolites, but natural plant populations cannot meet the commercial demand due to low accumulation volume. Hairy root cultures induced by plant interaction with Rhizobium rhizogenes are a practical solution for producing valuable secondary metabolites at low cost and without seasonal variations. Metabolic engineering in hairy roots, including overexpression and silencing of genes and transcription factor overexpression, can enhance metabolite production. This review focuses on alkaloids and discusses future prospects for meeting production demands through metabolic engineering in hairy roots.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Nisar Ahmad, Palwasha Khan, Abdullah Khan, Maliha Usman, Mohammad Ali, Hina Fazal, Durrishahwar, Muhammad Nazir Uddin, Christophe Hano, Bilal Haider Abbasi
Summary: This study investigated the effect of Cuscuta reflexa extract as a biotic elicitor on the biomass accumulation and production of antioxidant secondary metabolites in submerged adventitious root cultures of Stevia rebaudiana. The results showed that Cuscuta reflexa extract moderately improved biomass accumulation and enhanced the synthesis of phenolics, flavonoids, and antioxidant activities.
Review
Plant Sciences
Diptesh Biswas, Avijit Chakraborty, Swapna Mukherjee, Biswajit Ghosh
Summary: This study highlights the importance of hairy roots in the production of secondary metabolites in Solanaceous plants. It provides an overview of modern biotechnological techniques used to enhance the production of these metabolites in hairy root cultures.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Seungeun Baek, Jong-Eun Han, Thanh-Tam Ho, So-Young Park
Summary: This study tested the efficiency of hairy root induction in Centella asiatica using leaf and petiole explants. The results showed that petiole-derived hairy roots had the highest growth index and triterpenoid concentration. The findings suggest that hairy root culture of C. asiatica can be scaled up for mass production of triterpenoids.
Article
Plant Sciences
Minsol Choi, Jiwon Yoon, So Hwi Yang, Jae Kwang Kim, Sang Un Park
Summary: In this study, the optimal conditions for the hairy root culture of Salvia plebeia were determined, and its growth and accumulation of phenolic compounds were evaluated. The results showed that hairy roots cultured under optimal conditions exhibited enhanced phenolic compound accumulation and antioxidant activity, making them potential sources of antioxidants.
Article
Biochemical Research Methods
Ignacio Jimenez Amezcua, Sergio Rivas Blas, Marina Diez Municio, Ana Cristina Soria, Ana Isabel Ruiz Matute, Maria Luz Sanza
Summary: A new multianalytical methodology based on gas chromatography and liquid chromatography coupled to mass spectrometry has been proposed to evaluate frauds affecting the composition of Coleus forskohlii root supplements. The methodology successfully detected low concentrations of forskolin and the replacement of C. forskohlii by other vegetable sources in the supplements.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Plant Sciences
Khwanlada Kobtrakul, Dolly Rani, Asma Binalee, Pattarapol Udomlarp, Tatiya Srichai, Wanchai De-Eknamkul, Sornkanok Vimolmangkang
Summary: Cannabis sativa L. (hemp) is widely used for medicinal, food, and textile purposes. This study focused on the roots of hemp, which have received less attention than other parts. By using hairy root cultures and treating them with salicylic acid, the levels of triterpenoids friedelin and epifriedelanol in the roots were significantly increased. These findings suggest that hairy root cultures with optimized elicitation could be an alternative method to produce valuable triterpenoids.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Tong Wu, Sandra M. Kerbler, Alisdair R. Fernie, Youjun Zhang
Summary: Synthetic biology in plants has attracted increasing attention, and plant cells may be a promising alternative for the production of high-value metabolites. Commercial plant suspension cell cultures have been successfully used to produce valuable metabolites in a safe and cost-effective manner, but the variety of metabolites biosynthesized using plant platforms is currently limited.
PLANT COMMUNICATIONS
(2021)
Review
Biotechnology & Applied Microbiology
Christoph Wawrosch, Sergey B. Zotchev
Summary: Medicinal plants have been used for centuries, with bioactive plant secondary metabolites now serving as important drugs. Biotechnological manufacturing offers advantages over traditional methods, though commercialization is still in progress. Advances in biosynthetic pathway elucidation and genetic manipulation show promise for utilizing plant metabolic engineering.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Agricultural Engineering
Mahesh Kumar Badhepuri, M. Manokari, M. Cokul Raj, Phanikanth Jogam, Abhijit Dey, Mohammad Faisal, Abdulrahman A. Alatar, Nirmal Joshee, Nageswara Rao Singisala, Mahipal S. Shekhawat
Summary: A novel and effective direct shoot regeneration technique was developed for the medicinal plant Coleus forskohlii. Meta-Topolin was found to be more efficient than BAP in promoting direct organogenesis. The regeneration process showed distinct phases of shoot formation. The developed protocol can be used for the commercial production of C. forskohlii.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Agricultural Engineering
Kajal Kumari, Sanjeev Kumar, Prashant, Amrit Kumar Jha, Nitish Kumar
Summary: This review paper discusses the application of biotechnological techniques for enhancing the production of secondary metabolites in O. sanctum. By using modern technology, the pharmaceutical yield of this plant can be improved, and studies on gene transfer and genome structure have been conducted.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Review
Biochemistry & Molecular Biology
Marouane Mohaddab, Younes El Goumi, Monica Gallo, Domenico Montesano, Gokhan Zengin, Abdelhakim Bouyahya, Malika Fakiri
Summary: Medicinal plants produce bioactive secondary metabolites that are widely used in various industries. The biotechnological methods of cell culture offer a promising approach to increase the production of secondary metabolites.
Review
Plant Sciences
Irfan Bashir Ganie, Zishan Ahmad, Anwar Shahzad, Alexandra Zaushintsena, Olga Neverova, Svetlana Ivanova, Adla Wasi, Sabaha Tahseen
Summary: Centella asiatica, also known as Gotu kola, is a medicinal plant with a wide range of active metabolites. However, limited cultivation and overexploitation have led to severe depletion of its resources. This review provides up-to-date information on biotechnological tools for in vitro propagation, secondary metabolite production, and biosynthetic mechanism of Centella asiatica.
Article
Chemistry, Medicinal
Xiao Huang, Pengtao You, Yulin Chen, Tian Zhang, Ling Gong, Pencheng Zou, Weisong Li, Lei Wang, Yanwen Liu, Kun Yu, Bisheng Huang
Summary: This study characterized the morphology and structure of trichomes from Coleus forskohlii and analyzed the chemical qualities of their secretory materials, identifying four distinct types of trichomes and major compounds like polyphenols, flavonoids, lipids, and terpenes. Analysis of essential oils from the trichomes revealed oxygen-containing sesquiterpenes and monoterpenes as the main components.
PHARMACOGNOSY MAGAZINE
(2021)
