Article
Chemistry, Inorganic & Nuclear
Juan Sanz Garcia, Marie Gaschard, Isabelle Navizet, Mehdi Sahihi, Siden Top, Yong Wang, Pascal Pigeon, Anne Vessieres, Michele Salmain, Gerard Jaouen
Summary: The family of ferrocifens, derived from the anti-oestrogen tamoxifen, exhibit a broad antitumor activity by generating reactive oxygen species and electrophilic quinone methides. In addition to their known targets, cathepsin B was unexpectedly identified as a new target for ferrocifens.
EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
(2022)
Article
Infectious Diseases
Mathieu Nicolas, Bruno Beito, Marta Oliveira, Maria Tudela Martins, Bruno Gallas, Michele Salmain, Souhir Boujday, Vincent Humblot
Summary: This review summarizes the applications of antimicrobial peptides (AMPs) in preventing bacterial adhesion and biofilm formation, as well as the immobilization techniques of AMPs in biomedical applications. The progress towards clinical approval of AMPs in antibiotherapy is also discussed.
Article
Chemistry, Medicinal
Yong Wang, Pascal Pigeon, Wei Li, Jiangkun Yan, Patrick M. Dansette, Mohamed Othman, Michael J. McGlinchey, Gerard Jaouen
Summary: Ferrociphenols, compounds characterized by a biologically active redox motif, have anticancer properties. This study synthesized a variety of ferrociphenols with nitrogen-containing substituents and investigated their structure-activity relationships. Oxidative metabolism experiments and ROS-related bioactivity measurements were conducted to explore the cytotoxicity of imidoferrociphenols. Additionally, dimerization phenomenon observed in the crystal structure of a ferrociphenol may affect its antitumor activity.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Yacine Mazouzi, Fadoua Sallem, Francesca Farina, Alexis Loiseau, Natayme Rocha Tartaglia, Morgane Fontaine, Atul Parikh, Michele Salmain, Christian Neri, Souhir Boujday
Summary: This study combines quartz crystal microbalance with dissipation (QCM-D) and nanoplasmonic sensing (NPS) to provide a facile method for tracking the viscoelastic properties of small EVs, analyzing EVs related to the Huntington's disease gene. The results suggest that the mass and viscoelastic dissipation of EVs can serve as potent biomarkers.
Article
Biochemistry & Molecular Biology
Pascal Pigeon, Marie Gaschard, Mohamed Othman, Michele Salmain, Gerard Jaouen
Summary: The addition of imide entities to the ferrociphenol scaffold enhanced cytotoxic activity. By converting alpha-hydroxylactams to N-acyliminium ions, various substituents were grafted to the imide motif, resulting in diverse cytotoxic activities on breast cancer cell lines.
Article
Biochemistry & Molecular Biology
Charles Fayolle, Pascal Pigeon, Nathalie Fischer-Durand, Michele Salmain, Olivier Buriez, Anne Vessieres, Eric Labbe
Summary: The first fluorescent ferrociphenol derivative has been synthesized, and its fluorescence properties were found to be dependent on the redox state of the ferrocene group. This compound could serve as a useful tool to study the biodistribution and redox state of ferrociphenols in cancer cells.
Article
Chemistry, Analytical
Mona Soroush, Walid Ait Mammar, Axel Wilson, Hedayatollah Ghourchian, Michele Salmain, Souhir Boujday
Summary: We developed a magneto-plasmonic biosensor for immunodetection of antigens in small sample volumes. The biosensor utilized gold nanoparticles and magnetic beads conjugated with antibodies as capture and detection probes, respectively. The sandwich immunocomplex formed by the target analyte and detection probe was separated using a magnetic field, and the signal was measured by UV-Visible spectroscopy. The assay was successfully applied in a microfluidic system for quantification of the analyte.
Article
Chemistry, Multidisciplinary
Pierre Idlas, Guillaume Bastiat, Pascal Pigeon, Michael J. McGlinchey, Nolwenn Lautram, Anne Vessie, Gerard Jaouen, Catherine Passirani, Elise Lepeltier
Summary: By modifying the structure of ferrocifens, the drug loading in lipid nanocapsules can be improved, and the formation of a gel can be achieved. This has potential implications for the treatment of multidrug-resistant cancers and glioblastoma.
Article
Chemistry, Applied
Marcel Annereau, Franck Martial, Jeremy Forte, Geoffrey Gontard, Sebastien Blanchard, Heloise Dossmann, Michele Salmain, Vincent Corce
Summary: A series of manganese(I) tricarbonyl complexes were synthesized and characterized, which could be easily obtained from a common dimeric structure. These complexes, when reacted with imidazole derivatives, led to the formation of monomeric complexes with various functional groups. Photoactivation of these complexes resulted in the release of CO, with the mechanism of CO release explored through spectroscopic and computational methods. A manganese complex containing a more elaborate imidazole ligand was successfully synthesized, allowing for its direct incorporation in peptides.
APPLIED ORGANOMETALLIC CHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Sunyoung Hur, Christophe Methivier, Axel Wilson, Michele Salmain, Souhir Boujday, Ali Miserez
Summary: The protein MrCP20 in the calcareous base plate of the acorn barnacle Megabalanus rosa plays a role in regulating biomineralization and growth of the barnacle. It affects the kinetics of crystal nucleation and growth, as well as stabilizes a specific polymorph of calcium carbonate. The results provide insights into the molecular mechanisms of biomineralization and other functional roles of MrCP20.
ACS APPLIED BIO MATERIALS
(2023)
Review
Chemistry, Inorganic & Nuclear
Michele Salmain, Benoit Bertrand
Summary: The medicinal chemistry of gold has seen rapid progress since the 2000s, with new modes of action emerging for gold-based drug candidates. Previously, the mechanisms of gold complexes were limited to direct coordination or supramolecular interactions, but recent research has revealed additional modalities such as photo-induced cytotoxicity, selective protein modification, and in cellulo catalysis.
EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
(2023)
Review
Oncology
Michele Salmain, Marie Gaschard, Milad Baroud, Elise Lepeltier, Gerard Jaouen, Catherine Passirani, Anne Vessieres
Summary: The identification of biological targets is crucial in understanding the mechanism of action of anticancer drugs. This review examines the relationship between the inhibition of thioredoxin reductase (TrxR), a key enzyme in maintaining cell redox balance, and the cytotoxic effects of two groups of organometallic complexes. The results show that these complexes interact differently with TrxR at the molecular level, and the majority of their antiproliferative activity stems from the interaction with multiple targets. The review also highlights the potential of a multi-targeting approach to treat multidrug-resistant (MDR) tumors.
Article
Biochemistry & Molecular Biology
Pascal Pigeon, Feten Najlaoui, Michael James McGlinchey, Juan Sanz Garcia, Gerard Jaouen, Stephane Gibaud
Summary: This study aimed to determine the most suitable cyclodextrins (CDs) for solubilizing a patented succinimido-ferrociphenol compound (SuccFerr) with powerful anticancer activity but low water solubility. Phase solubility experiments and computational modeling were conducted on various CDs. The modeling and experiments suggest that supramolecular assemblies of SuccFerr with two CDs, particularly randomly methylated β-cyclodextrins (RAME-β-CDs), are predominant and superior. Additionally, the modeling reveals competing combinations of inserted moieties of SuccFerr and the contribution of ferrocene to high stabilization through atypical hydrogen bonding with CD hydroxyl groups.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)