Journal
JOURNAL OF MEDICINAL CHEMISTRY
Volume 52, Issue 8, Pages 2531-2542Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jm801475n
Keywords
-
Categories
Funding
- Scottish Funding Council
- Biotechnology and Biological Sciences Research Council [BBS/B/14434]
- Wellcome Trust [082596, 083481]
- Inpharmatica Ltd
- Biotechnology and Biological Sciences Research Council [BBS/B/14434] Funding Source: researchfish
Ask authors/readers for more resources
The nonmevalonate route to isoprenoid biosynthesis is essential in Gram-negative bacteria and apicomplexan parasites. The enzymes of this pathway are absent from mammals, contributing to their appeal as chemotherapeutic targets. One enzyme, 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase (IspF), has been validated as a target by genetic approaches in bacteria. Virtual screening against Escherichia coli IspF (EcIspF) was performed by combining a hierarchical filtering methodology with molecular docking. Docked compounds were inspected and 10 selected for experimental validation. A surface plasmon resonance assay was developed and two weak ligands identified. Crystal structures of EcIspF complexes were determined to support rational ligand development. Cytosine analogues and Zn2+-binding moieties were characterized. One of the putative Zn2+-binding compounds gave the lowest measured K-D to date (1.92 +/- 0.18 mu M). These data provide a framework for the development of IspF inhibitors to generate lead compounds of therapeutic potential against microbial pathogens.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available