Article
Immunology
Leila dos Santos Moura, Vinicius Santana Nunes, Antoniel A. S. Gomes, Ana Caroline de Castro Nascimento Sousa, Marcos R. M. Fontes, Sergio Schenkman, Nilmar Silvio Moretti
Summary: The research highlights the involvement of lysine acetylation in the oxidative stress response of T. cruzi, especially through the mitochondrial lysine deacetylase TcSir2rp3 regulating the activity of mitochondrial superoxide dismutase A (TcSODA). The study also shows that acetylation of K97 in TcSODA plays a key role in modulating enzyme activity and maintaining redox homeostasis in trypanosomatids. Additionally, the interaction between TcSir2rp3 and TcSODA contributes to the understanding of mechanisms used by T. cruzi to progress during infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Parasitology
Jessica Hickson, Lucas Felipe Almeida Athayde, Thaina Godinho Miranda, Policarpo Ademar Sales Junior, Anderson Coqueiro Dos Santos, Lucia Maria da Cunha Galvao, Antonia Claudia Jacome da Camara, Daniella Castanheira Bartholomeu, Rita de Cassia Moreira de Souza, Silvane Maria Fonseca Murta, Laila Alves Nahum
Summary: This study investigates the genes coding for iron superoxide dismutase (FeSOD) in T. cruzi strains from an evolutionary perspective. The results reveal the molecular biodiversity of T. cruzi FeSODs and identify potential drug targets.
PARASITES & VECTORS
(2022)
Article
Biochemistry & Molecular Biology
Carolina Prolo, Damian Estrada, Lucia Piacenza, Diego Benitez, Marcelo A. Comini, Rafael Radi, Maria Noel Alvarez
Summary: This study shows that the lack of superoxide radical produced by Nox2-deficient macrophages leads to increased susceptibility to T. cruzi infection in a murine model. Reconstitution of intraphagosomal superoxide radical formation in these macrophages restores the control of infection. The presence of Nox2-derived superoxide radical plays a crucial role in controlling early phase T. cruzi infection in a murine model of Chagas disease.
Article
Chemistry, Analytical
Caroline G. Sanz, Daniel N. Crisan, Ricardo J. B. Leote, Melania Onea, Madalina M. Barsan
Summary: In this study, a biosensor for quantifying superoxide in cell culture media was successfully developed using gold nanoparticles and superoxide dismutase bioconjugates. The optimized biosensor achieved a sensitivity value of 41.2 nAμMcm(-2), a detection limit of 1.0μM, and showed excellent operational stability, indicating great potential for practical applications.
Article
Chemistry, Medicinal
Ivan Beltran-Hortelano, Richard L. Atherton, Mercedes Rubio-Hernandez, Julen Sanz-Serrano, Veronica Alcolea, John M. Kelly, Silvia Perez-Silanes, Francisco Olmo
Summary: Chagas disease, caused by Trypanosoma cruzi, is the most important parasitic infection in Latin America. The current treatments are limited by high toxicity and low efficacy, highlighting the urgent need for more effective and less toxic therapeutic agents. Through the synthesis of 69 new analogues, researchers identified derivative 3c as a promising candidate with excellent trypanocidal properties and a synergistic mode of action with the reference drug benznidazole.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Ivan Beltran-Hortelano, Veronica Alcolea, Maria Font, Silvia Perez-Silanes
Summary: This article provides an overview of validated targets involved in various pathways of the Trypanosoma cruzi parasite and explores the molecular basis for finding new effective treatments for Chagas disease. The review also compiles all reported inhibitors against these targets, serving as a reference for future research in this field.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Fisheries
Min Yang, Luchuanyang Sun, Yasunosuke Kawabata, Takahiro Maegawa, Shigeto Taniyama, Katsuyasu Tachibana, Katsuya Hirasaka
Summary: Balenine, an endogenous imidazole dipeptide composed of beta-alanine and 3-methyl-l-histidine, is found mainly in muscles and the brain. Studies have shown that balenine exhibits antioxidant activity by increasing SOD activity without affecting other antioxidant enzymes. This suggests that balenine contributes to antioxidant effects through activation of SOD.
Article
Chemistry, Applied
Yang Zhou, Yanmei Li, Shaoguang Wu, Yuling Zhao, Tianzhi Yu, Wenming Su, Hailin Ma, Long Qian, Ruidong Wang
Summary: Two benzo[d]imidazole/triazatruxene hybrid compounds were synthesized and their thermal stabilities, photophysical, and electrochemical properties were investigated. These compounds showed good solubility and thermal stability, with the non-doped OLED device based on 2,7,12-TBI-TAT displaying the best electroluminescence performance.
Article
Health Care Sciences & Services
Irina A. Mednova, Liudmila P. Smirnova, Alisa R. Vasilieva, Daria Kazantseva, Elena Epimakhova, Nina M. Krotenko, Arkadiy Semke, Svetlana A. Ivanova
Summary: This study demonstrates that patients with schizophrenia have SOD activity in their IgG, and the highest activity is observed in patients in therapeutic remission. The mechanism of SOD activity in IgG is similar to classical enzyme catalysis, with a higher affinity for the substrate than classical SOD enzyme.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Yanwei Wang, Jiahui Zhao, Yipeng Xu, Cimin Tao, Jie Tong, Yingjie Luo, Yong Chen, Xuesong Liu, Tengfei Xu
Summary: This study integrated metabolomics and chemical proteomics approaches to comprehensively identify the potential target proteins affected by Bap in liver cells. The results showed that Bap weakened the antioxidant capacity by destroying the activities of SOD3 and GPX4, providing a new understanding of the mechanism of hepatotoxicity induced by Bap.
Review
Biochemistry & Molecular Biology
Alvaro de Obeso Fernandez del Valle, Christian Quintus Scheckhuber
Summary: This article reviews the role of different SODs in the development and pathogenicity of various eukaryotic microorganisms, including fungi, Aspergillus spp., parasitic protozoa, and pathogenic amoebae. SODs play essential roles in these microorganisms, participating in processes such as development, host infection, propagation, and control of gene expression.
Article
Immunology
Gerd Wallukat, Fernando Antonio Botoni, Manoel Otavio da Costa Rocha, Vitoria Louise, Johannes Mueller, Andre Talvani
Summary: The study found that dogs infected by Trypanosoma cruzi generate agonistic beta 1ARAb, beta 2ARAb, and M2RAb antibodies. Different strains of T. cruzi infection may impact the antibody activities. In cardiomyocytes culture, the antibodies recognized epitopes similar to those seen in human chronic Chagas cardiomyopathy.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Medicinal
Tai-Lin Chen, Anilkumar S. Patel, Vicky Jain, Ramajayam Kuppusamy, Yi-Wen Lin, Ming-Hon Hou, Tsann-Long Su, Te-Chang Lee
Summary: The design of hybrid molecules with antiangiogenic and DNA cross-linking activities showed potential broad-spectrum anticancer effects, with compound 19a being a promising candidate. Using a benzology approach to improve bioavailability, these hybrids could be formulated for intravenous and oral administration, indicating a helpful strategy for enhancing combination therapy efficacy.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Chemistry, Organic
Gleiston G. Dias, Esther R. S. Paz, Juliana Y. Kadooca, Adao A. Sabino, Luiz A. Cury, Kohei Torikai, Carlos A. de Simone, Felipe Fantuzzi, Eufranio N. da Silva Junior
Summary: Rhodium(III) catalysis facilitated the C-H/N-H alkyne annulation of nonsymmetric imidazole derivatives, leading to the synthesis of rigid pi-extended imidazole derivatives with outstanding fluorescence. The study also revealed the photophysical aspects and reaction mechanism through computational calculations, providing an efficient and versatile tool for the synthesis of fluorescent compounds with a wide range of chemical and biological applications.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Ecology
Bruno Serra de Lacerda Valverde, Heidi Samantha Moraes Utsunomiya, Cleoni dos Santos Carvalho, Lilian Franco-Belussi, Classius de Oliveira
Summary: Studies on anurans have been conducted for over 40 years to evaluate the adverse effects of benzo(alpha)pyrene (B alpha P), showing genotoxic, hepatotoxic, and immunotoxic effects. This study aimed to assess whether the route of administration of B alpha P influences its effects on hepatic biomarkers, with morphological biomarkers being more significantly affected than biochemical ones. The exposure pathway and time both modulate the effects of B alpha P on hepatic biomarkers in anurans.
Article
Chemistry, Medicinal
Ruben Martin-Escolano, Daniel Molina-Carreno, Estefania Delgado-Pinar, Alvaro Martin-Montes, M. Paz Clares, Encarnacion Medina-Carmona, Javier Pitarch-Jarque, Javier Martin-Escolano, Maria Jose Rosales, Enrique Garcia-Espana, Manuel Sanchez-Moreno, Clotilde Marin
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2019)
Article
Chemistry, Medicinal
Gisele E. Miana, Sergio R. Ribone, Domingo M. A. Vera, Manuel Sanchez-Moreno, Maria R. Mazzieri, Mario A. Quevedo
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2019)
Article
Microbiology
Lissette Retana Moreira, Daniel Vargas Ramirez, Fatima Linares, Alexa Prescilla Ledezma, Annette Vaglio Garro, Antonio Osuna, Jacob Lorenzo Morales, Elizabeth Abrahams Sandi
Article
Biochemistry & Molecular Biology
Lissette Retana Moreira, Alexa Prescilla-Ledezma, Alberto Cornet-Gomez, Fatima Linares, Ana Belen Jodar-Reyes, Jorge Fernandez, Ana Karina Ibarrola Vannucci, Luis Miguel De Pablos, Antonio Osuna
Summary: This study characterized the proteomes and nanomechanical properties of extracellular vesicles released by different life stages of Trypanosoma cruzi, the etiologic agent of Chagas disease. The findings revealed significant differences in surface molecular cargos of vesicles between trypomastigotes and epimastigotes, with trypomastigotes exhibiting higher adhesion properties. This suggests a remarkable surface remodeling throughout the parasite's life cycle, potentially impacting the ability of extracellular vesicles to participate in cell communication in diverse infection niches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Infectious Diseases
Ana K. Ibarrola-Vannucci, Luis M. De Pablos, Lissette Retana-Moreira, Alberto Cornet-Gomez, Teresa Cruz-Bustos, Alejandro G. Schijman, Jose L. Ramirez, Susana Vilchez, Antonio Osuna
Summary: Chagas disease is a neglected tropical disease with no available vaccine and limited treatment options. The study on T. cruzi PHBs reveals their pleiotropic function in different parasite stages and highlights their potential as therapeutic targets. PHB2 deletion has a lethal effect on the parasite, while PHBs play roles in metacyclogenesis and ROS detoxification.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Infectious Diseases
Katalina Cruz, Antonio Marcilla, Patrick Kelly, Michel Vandenplas, Antonio Osuna, Maria Trelis
Summary: Embryonated eggs of Trichuris trichiura play a crucial role in stimulating the immune system of the definitive host. Studies on the proteome and antigenic profile of non-embryonated eggs provide valuable information for potential immunodiagnostics and immunomodulators. The proteins identified in the study are involved in various biological functions, including lipid transport, energy metabolism, and muscle motility.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Ecology
Maria Buendia-Abad, Mariano Higes, Raquel Martin-Hernandez, Laura Barrios, Aranzazu Meana, Alberto Fernandez Fernandez, Antonio Osuna, Luis Miguel De Pablos
Summary: The study revealed the impact of cell culture passages on the pathogenicity of L. passim in honeybees, with the newly obtained C1 strain showing higher virulence, providing further evidence of its negative effects on honeybee health.
INTERNATIONAL JOURNAL FOR PARASITOLOGY-PARASITES AND WILDLIFE
(2021)
Article
Biochemistry & Molecular Biology
Alberto Fernandez-Fernandez, Antonio Osuna, Susana Vilchez
Summary: A 98.1 Kb genomic region from B. pumilus 15.1, isolated as an entomopathogen towards C. capitata, has been characterized to identify potential virulence factors. This study differentiates two regions responsible for producing a phage-like particle and a novel phage, respectively. The phage-like particle exhibits bacteriocin activity towards other B. pumilus strains, and the impact of both the phage-like particles and the phage on the toxicity towards C. capitata is evaluated.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Veterinary Sciences
Katalina Cruz, Tatiana M. Corey, Michel Vandenplas, Maria Trelis, Antonio Osuna, Patrick J. Kelly
Summary: Limited data is available on the efficacy of antiparasitic treatments and husbandry methods for controlling nematode infections in captive populations of African green monkeys. A study found that a treatment regimen of ivermectin and albendazole, coupled with good husbandry practices, effectively controlled nematode infections in AGMs, although some individuals were reinfected.
ONDERSTEPOORT JOURNAL OF VETERINARY RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Alexa Prescilla-Ledezma, Fatima Linares, Mariano Ortega-Munoz, Lissette Retana Moreira, Ana Belen Jodar-Reyes, Fernando Hernandez-Mateo, Francisco Santoyo-Gonzalez, Antonio Osuna
Summary: This study utilizes AFM-based single molecule-force spectroscopy to detect and locate functional trans-sialidases on the surface of extracellular vesicles released by tissue-culture cell-derived trypomastigotes. By engineering AFM cantilevers with functionalized tips bearing anti-TS antibodies, molecular recognition of trans-sialidases using antibody-antigen interaction is achieved. The results demonstrate that the extracellular vesicles isolated from tissue-culture cell-derived trypomastigotes are enriched in trans-sialidases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Entomology
Jessica Carreira De Paula, Kevin Doello, Cristina Mesas, Garyfalia Kapravelou, Alberto Cornet-Gomez, Francisco Jose Orantes, Rosario Martinez, Fatima Linares, Jose Carlos Prados, Jesus Maria Porres, Antonio Osuna, Luis Miguel de Pablos
Summary: The study utilized Micro-CT and novel staining methods to characterize the morphoanatomical features of the worker honeybee abdomen, identifying a new cell type known as ventricular telocyte. This method is proposed to be useful in further investigating the structure of honeybee abdomen under various environmental conditions. Continuous improvements in morphological and histochemical analyses of Apis mellifera could enhance the understanding of their anatomy and physiology at cellular and tissue levels.
Article
Biology
Lissette Retana Moreira, Maria Fernanda Steller Espinoza, Natalia Chacon Camacho, Alberto Cornet-Gomez, Giovanni Saenz-Arce, Antonio Osuna, Bruno Lomonte, Elizabeth Abrahams Sandi
Summary: Extracellular vesicles produced by Naegleria fowleri, a free-living amoeba, have been characterized for the first time. These vesicles may play a significant role in the invasion and damage to the brain, induce specific cellular responses, and could be used for diagnostic purposes. The complexity of the vesicles produced by N. fowleri suggests their potential relevance in the release of virulence factors involved in pathogenicity.
Article
Microbiology
Maria Teresa Galan-Puchades, Mercedes Gomez-Samblas, Antonio Osuna, Sandra Saez-Duran, Ruben Bueno-Mari, Marius V. Fuentes
Summary: Recently, the rat lungworm was found for the first time in rats in the sewer system of Valencia, Spain. Further analysis revealed that 8 out of 94 rats were infected, with the highest prevalence of infection (20%) in rats trapped in orchards surrounding the city. The risk of acquiring neuroangiostrongylosis should be minimal if proper precautions are taken.
Article
Multidisciplinary Sciences
Noelia Lozano, Mercedes Gomez Samblas, Eva Calabuig, Maria Jose Gimenez Marti, Maria Dolores Gomez Ruiz, Jose Miguel Sahuquillo Arce, Sergio Sequera-Arquelladas, Jose Miguel Molina Moreno, M. Trelis, Antonio Osuna
Summary: This study evaluated the use of nucleic acids from circulating exovesicles and cell-free DNA (cfDNA) to confirm the presence of parasite infection in chronically ill patients. The results show that both exovesicle DNA and cfDNA can be used to detect parasitism in these patients.
Article
Multidisciplinary Sciences
Alexa Prescilla Ledezma, Roberto Blandon, Alejandro G. Schijman, Alejandro Benatar, Azael Saldana, Antonio Osuna
