Article
Food Science & Technology
Divya Kode, Ramakrishna Nannapaneni, Sam Chang
Summary: Between January and July 2021, there were 30 recalls in the U.S. due to potential Listeria monocytogenes contamination in various food products. The emergence of antibiotic resistance in L. monocytogenes poses a risk to public health. Research indicates that biocides used in food processing may contribute to the development of antibiotic resistance in L. monocytogenes.
Article
Microbiology
Jing Wang, Zhan He, Mingzhu Cui, Jing Sun, Lingli Jiang, Nanxi Zhuang, Fuxin Zhu, Xian Zhang, Houhui Song, Changyong Cheng
Summary: HIF-1 alpha plays a role in the invasion of Listeria monocytogenes into hypoxic tilapia by upregulating c-Met expression and promoting the internalization of InlB. This study reveals the regulatory mechanisms of HIF-1 alpha in L. monocytogenes infection and provides insights for the prevention and control of this pathogen in aquaculture.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Jing Wang, Zhan He, Mingzhu Cui, Jing Sun, Lingli Jiang, Nanxi Zhuang, Fuxin Zhu, Xian Zhang, Houhui Song, Changyong Cheng
Summary: HIF-1a is upregulated in hypoxic tilapia, promoting the invasion of Listeria monocytogenes by upregulating c-Met expression, which mediates the internalization of InlB. Hypoxic conditions and bacterial burden counts were found to have no effect on the infection of L. monocytogenes in hypoxic fish when using ?InlB strain.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Divya Kode, Ramakrishna Nannapaneni, Mohit Bansal, Sam Chang, Wen-Hsing Cheng, Chander S. Sharma, Aaron Kiess
Summary: The study found that Listeria monocytogenes developed low-level tolerance to the fluoroquinolone antibiotic ciprofloxacin after sublethal adaptation to quaternary ammonium compound (QAC), showing increased short-range MIC, growth rate, and survival in ciprofloxacin for certain strains. However, not all strains exhibited increased tolerance after QAC adaptation, suggesting the potential formation of low-level ciprofloxacin-tolerant subpopulations in certain strains when exposed to residual QAC concentrations.
Article
Microbiology
Yaroslava Chalenko, Olga Kolbasova, Elena Pivova, Mariam Abdulkadieva, Olga Povolyaeva, Egor Kalinin, Denis Kolbasov, Svetlana Ermolaeva
Summary: This study investigated the interaction between L. monocytogenes and sheep cells, and found that the invasion efficiency was influenced by the InlB factor. Different InlB isoforms may contribute to the hypervirulence of certain clonal groups towards ruminants, and L. monocytogenes can effectively multiply once it enters sheep cells.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Zuhua Yu, Yingying Zhao, Ke Ding, Lei He, Chengshui Liao, Jing Li, Songbiao Chen, Ke Shang, Jian Chen, Chuan Yu, Chunjie Zhang, Yinju Li, Shaohui Wang, Yanyan Jia
Summary: This study demonstrated that Listeria monocytogenes (LM) exhibits antitumor effects by inducing autophagy, and the combination of LM and the autophagy inhibitor chloroquine (CQ) enhances the antitumor activity of LM both in vitro and in vivo.
Article
Food Science & Technology
Ekaterina K. Psareva, Elena A. Liskova, Irina Razheva, Yulia K. Yushina, Maria A. Grudistova, Nadezda A. Gladkova, Eugene A. Potemkin, Pavel A. Zhurilov, Elena Sokolova, Pavel A. Andriyanov, Olga L. Voronina, Denis Kolbasov, Svetlana A. Ermolaeva
Summary: The study identified high molecular diversity among Listeria monocytogenes strains isolated in Central European Russia, with significant differences observed between strains of different lineages. The closure of the Russian market for imported food in 2019-2020 had a notable impact on the types and distribution of isolated strains.
Article
Food Science & Technology
Han Bin Lee, Ki Hwan Kim, Gweon Ah Kang, Kwang-Geun Lee, Seok-Seong Kang
Summary: This study aimed to investigate the effect of bacterial lysates (BLs) extracted from Pediococcus acidilactici on the biofilm formation, adhesion, and invasion of Listeria monocytogenes. The results showed that BLs inhibited biofilm formation and downregulated the expression of biofilm-associated genes. Additionally, BLs effectively inhibited bacterial adhesion and invasion, and downregulated the transcription of genes related to adhesion and invasion. Therefore, BLs have the potential to reduce health risks associated with Listeria monocytogenes.
Article
Microbiology
Elton Burnett, Zuzana Kucerova, Molly Freeman, Sophia Kathariou, Jessica Chen, Monica Smikle
Summary: This study investigated the genetic characteristics and clonal relatedness of L. monocytogenes isolates from two Jamaican meat processing facilities. The majority of isolates belonged to lineage I, with a single isolate from lineage II. Pulsed-field gel electrophoresis and whole-genome sequencing revealed the presence of several pulsotypes and clonal complexes. Virulence-associated genes and resistance cassettes were also detected. The results suggest the presence of persistent strains of L. monocytogenes in the meat-producing environments, emphasizing the need for robust surveillance strategies.
Article
Biotechnology & Applied Microbiology
Cristina Rodriguez-Melcon, Victor Serrano-Galan, Rosa Capita, Carlos Alonso-Calleja
Summary: The aim of this research was to investigate the effect of subinhibitory concentrations of disinfectants on the resistance of Listeria monocytogenes to tetracycline. The results showed that exposure to subinhibitory doses of the disinfectants could lead to increased resistance to tetracycline. The use of disinfectants at subinhibitory concentrations should be avoided to prevent the development of resistance.
Article
Food Science & Technology
Arkadiusz Jozef Zakrzewski, Wioleta Chajecka-Wierzchowska, Anna Zadernowska
Summary: Ceviche is a traditional marinated raw fish dish commonly consumed in Pacific coastal countries. This study aimed to assess the viability of bacteria isolated from fish after the preparation of ceviche. The results showed that different strains of bacteria had varying survival rates and pathogenic potentials during the preparation process, and certain ingredients in ceviche exhibited antibacterial properties.
Article
Food Science & Technology
Jack Burnett, Sophie Tongyu Wu, Maxwell Voorn, Christopher Jordan, Clyde S. Manuel, Manpreet Singh, Haley F. Oliver
Summary: Our study on Listeria monocytogenes prevalence and persistence in retail produce environments revealed that some departments harbor persistent isolates of L. monocytogenes. Through re-training and deep cleaning, we were able to reduce the overall prevalence of L. monocytogenes, although only a few stores significantly reduced their prevalence. Factors such as the presence of foam squeegees, damaged equipment, or plumbing leaks predicted the effectiveness of the intervention.
Article
Microbiology
Junyaluck Rukit, Atsadang Boonmee, Teeratas Kijpornyongpan, Kan Tulsook, Jozsef Baranyi, Soraya Chaturongakul
Summary: This study aimed to investigate the roles of different sigma factors in Listeria monocytogenes invasion and growth inside host cells. The results showed that SigB is the key factor for invasion, while the absence of SigL may enhance host invasion.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Cell Biology
Sae-Hae Kim, Byeol-Hee Cho, Kwang Soon Kim, Yong-Suk Jang
Summary: C5a in the Peyer's patch enhances CD8(+) T cell responses by increasing C5/C5a levels. Single-cell RNA sequencing identifies dendritic cells expressing C5aR and lysozyme as key players in CD8(+) T cell immune induction.
Article
Food Science & Technology
Anderson C. Camargo, Adelle P. McFarland, Joshua J. Woodward, Luis Augusto Nero
Summary: This study examined the invasion, replication, and spread ability of Listeria monocytogenes isolates from Brazil in human intestinal epithelial cells. The results showed that serotype is a better predictor of cell invasion and spread compared with sample origin, and premature stop codons in the inlA gene were associated with decreased invasion ability in certain strains.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Microbiology
Ashira Lubkin, Warren L. Lee, Francis Alonzo, Changsen Wang, Jason Aligo, Matthew Keller, Natasha M. Girgis, Tamara Reyes-Robles, Rita Chan, Aidan O'Malley, Peter Buckley, Nikollaq Vozhilla, Marilyn T. Vasquez, Johnny Su, Michael Sugiyama, Stephen T. Yeung, Maryaline Coffre, Sofia Bajwa, Eric Chen, Patricia Martin, Sang Y. Kim, Cynthia Loomis, G. Scott Worthen, Bo Shopsin, Kamal M. Khanna, Daniel Weinstock, Anthony Simon Lynch, Sergei B. Koralov, P'ng Loke, Ken Cadwell, Victor J. Torres
CELL HOST & MICROBE
(2019)
Article
Immunology
Chance J. Cosgriff, Chelsea R. White, Wei Ping Teoh, James P. Grayczyk, Francis Alonzo
INFECTION AND IMMUNITY
(2019)
Article
Microbiology
Wei Ping Teoh, Zachary J. Resko, Sarah Flury, Francis Alonzo
JOURNAL OF BACTERIOLOGY
(2019)
Article
Immunology
Sarah Talley, Olga Kalinina, Michael Winek, Wonbeom Paik, Abigail R. Cannon, Francis Alonzo, Mashkoor A. Choudhry, Katherine L. Knight, Edward M. Campbell
JOURNAL OF IMMUNOLOGY
(2019)
Article
Immunology
Wonbeom Paik, Francis Alonzo, Katherine L. Knight
INFECTION AND IMMUNITY
(2020)
Article
Microbiology
Kelly M. Hines, Gloria Alvarado, Xi Chen, Craig Gatto, Antje Pokorny, Francis Alonzo, Brian J. Wilkinson, Libin Xu
Article
Multidisciplinary Sciences
Raiza R. Bonomo, Tyler M. Cook, Chaitanya K. Gavini, Chelsea R. White, Jacob R. Jones, Elisa Bovo, Aleksey Zima, Isabelle A. Brown, Lara R. Dugas, Eleonora Zakharian, Gregory Aubert, Francis Alonzo, Nigel A. Calcutt, Virginie Mansuy-Aubert
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Multidisciplinary Sciences
Wei Ping Teoh, Xi Chen, Irina Laczkovich, Francis Alonzo
Summary: During infection, pathogenic microbes such as Staphylococcus aureus adapt to the host's nutritional environment by scavenging necessary nutrients, including lipoic acid, and enzymes required for metabolism. The ability to acquire lipoic acid and unsaturated fatty acids from the host environment is crucial for the pathogen's virulence in different infection sites. The findings emphasize the importance of host lipid assimilation for bacterial survival and suggest challenges in targeting bacterial lipogenesis as an antibacterial strategy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
Xi Chen, Wei Ping Teoh, Madison R. Stock, Zachary J. Resko, Francis Alonzo
Summary: The diverse fatty acid composition in the bacterial cell envelope affects membrane fluidity and immune responses. Shifting away from branched chain fatty acids towards unsaturated fatty acids can disrupt infection dynamics and lead to immune activation. This study highlights the importance of acyl chain repertoire in bacterial infection outcomes and immune responses.
Editorial Material
Microbiology
Ivan C. Acosta, Francis Alonzo Iii
Summary: Tissue damage and persistent inflammation are distinct features of antibiotic-resistant chronic infections. In this study, Tang et al. demonstrate that anti-folate antibiotics can trigger the synthesis of a bacterial second messenger, leading to an excessive immune response and establishing a paradigm for chronic infection.
CELL HOST & MICROBE
(2022)
Article
Biochemistry & Molecular Biology
Laty A. Cahoon, Xiomarie Alejandro-Navarreto, Avinash N. Gururaja, Sam H. Light, Francis Alonzo, Wayne F. Anderson, Nancy E. Freitag
Summary: Listeria monocytogenes is a bacterium that can cause infections in susceptible hosts. The proteins PrsA1 and PrsA2 play different roles in the bacterium's pathogenesis, with PrsA2 being more important. The PieRS signal transduction system regulates the expression of PrsA1 and PrsA2, and its gene products are crucial for the bacterium's resistance to ethanol and survival in the gastrointestinal tract.
MOLECULAR MICROBIOLOGY
(2022)
Editorial Material
Microbiology
Francis Alonzo
Summary: The skin is a hostile environment for microbial growth, but commensal microbes and some opportunistic pathogens can survive within it. Coagulase-negative staphylococci (CoNS) are important constituents of the skin microbiome, and Staphylococcus epidermidis and Staphylococcus hominis are common colonizers of human skin. S. hominis is increasingly recognized as a beneficial commensal, and studying its interactions with other skin microbes, including pathogenic strains, has become of interest.
Article
Immunology
Madison R. Stock, Liwei Fang, Kaelie R. Johnson, Chance Cosgriff, Wei Ping Teoh, Francis Alonzo
Summary: The study reveals the critical role of MroQ in the maturation and activity of AIP in Agr-I, -II, and -IV strains, but not in Agr-III. MroQ may act as a mediator of cyclic peptide maturation.
INFECTION AND IMMUNITY
(2022)
Article
Immunology
Zachary J. J. Resko, Caleb M. M. Anderson, Michael J. J. Federle, Francis Alonzo III
Summary: The peptidoglycan of Staphylococcus aureus plays an important role in subverting host immune defenses and protecting against stressors. The glucosaminidase SagB processes peptidoglycan chains, which is crucial for IL-1 beta production. SagB-mediated IL-1 beta production is independent of traditional receptor engagement and caspase activity, suggesting a novel mechanism for IL-1 beta maturation.
INFECTION AND IMMUNITY
(2023)
Article
Microbiology
Emily L. Pruitt, Rutan Zhang, Dylan H. Ross, Nathaniel K. Ashford, Xi Chen, Francis Alonzo III, Matthew F. Bush, Brian J. Werth, Libin Xu
Summary: Staphylococcus aureus can synthesize fatty acids through its own pathway and also utilize host-derived fatty acids. This study investigated the substrate specificity of secreted lipases, the impact of human serum albumin and FASII inhibitor on fatty acid incorporation. It was found that Geh is the main lipase responsible for hydrolyzing cholesteryl esters, but other lipases can compensate for its function. The incorporation of exogenous fatty acids alters the lipidome, membrane fluidity, and ROS formation of S. aureus.