Article
Food Science & Technology
Silvia Guillen, Maria Marcen, Ester Fau, Pilar Manas, Guillermo Cebrian
Summary: This study aimed to investigate the growth rates, virulence, and other phenotypic characteristics of 23 Salmonella strains belonging to different serovars. The results showed significant differences among the strains in terms of growth rates, virulence, and biofilm-forming ability. However, there was no clear association between stress resistance and growth ability, biofilm formation ability (except for acid stress), or antibiotic resistance.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Microbiology
Yi-Jung Chang, Yi-Ching Chen, Nai-Wen Chen, Ying-Jie Hsu, Hsiao-Han Chu, Chyi-Liang Chen, Cheng-Hsun Chiu
Summary: The antimicrobial resistance of NTS in children has increased progressively in the past decade in Taiwan, with different serogroups exhibiting different resistance patterns. Infants showed the highest risk of acquiring a third-generation cephalosporin-resistant NTS infection, highlighting the need for continual surveillance among children with NTS in Taiwan.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Schola K. Peter, Joshua M. Mutiso, Mercy Ngetich, Cecilia Mbae, Samuel Kariuki
Summary: Non-typhoidal Salmonella (NTS) infections are a public health challenge in sub-Saharan Africa, particularly in Kenya. A study was conducted to determine the proportion of children exposed to Salmonella Enteritidis or Salmonella Typhimurium and associated factors in Mukuru, Kenya. The study found a high exposure to NTS among children below five years of age and identified water treatment methods and animal keeping as factors associated with exposure.
Article
Microbiology
Roderick M. Card, Thomas Chisnall, Ruhena Begum, Md Samun Sarker, Muhammad Sazzad Hossain, Md Shahjalal Sagor, Mohammad Asheak Mahmud, A. S. M. Ashab Uddin, Md Rezaul Karim, Johanna F. Lindahl, Mohammed Abdus Samad
Summary: This study identified multidrug-resistant non-typhoidal Salmonella in migratory birds in Bangladesh, highlighting the potential risk to public and animal health. The spread of these Salmonella strains in the environment can occur through direct contact, transmission to food-producing animals on farms, and dissemination via the birds' long-range migratory movements. Continued surveillance and implementation of good farm biosecurity practices can help mitigate these risks.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Carla L. Schwan, Sara Lomonaco, Leonardo M. Bastos, Peter W. Cook, Joshua Maher, Valentina Trinetta, Manreet Bhullar, Randall K. Phebus, Sara Gragg, Justin Kastner, Jessie L. Vipham
Summary: This study identified and characterized the genotypic and phenotypic AMR profiles of environmental S. enterica strains isolated from informal markets in Cambodia. The research revealed multiple AMR genes and resistances, contributing important data for understanding multidrug resistance profiles in the region.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Food Science & Technology
Silvia Guillen, Laura Nadal, Ignacio Alvarez, Pilar Manas, Guillermo Cebrian
Summary: The success of Salmonella as a foodborne pathogen can be attributed to its genetic diversity and adaptability, allowing it to survive in harsh conditions and cause disease. However, the development of stress resistance may come with a fitness cost for bacterial cells and can trigger changes in Salmonella virulence.
Article
Infectious Diseases
Kassiani Mellou, Mary Gkova, Emily Panagiotidou, Myrsini Tzani, Theologia Sideroglou, Georgia Mandilara
Summary: The study in Greece from 2003 to 2020 found a decline in the total number of samples of non-typhoidal salmonellosis, with S. Enteritidis being the most common serotype.
Article
Infectious Diseases
Jessica Z. Kubicek-Sutherland, Gary Xie, Migun Shakya, Priya K. Dighe, Lindsey L. Jacobs, Hajnalka Daligault, Karen Davenport, Loreen R. Stromberg, Zachary R. Stromberg, Qiuying Cheng, Prakasha Kempaiah, John Michael Ong'echa, Vincent Otieno, Evans Raballah, Samuel Anyona, Collins Ouma, Patrick S. G. Chain, Douglas J. Perkins, Harshini Mukundan, Benjamin H. McMahon, Norman A. Doggett
Summary: Non-typhoidal Salmonella (NTS) is a major global health concern, particularly in areas affected by malnutrition and comorbidities like HIV and malaria. This study identified a unique NTS isolate named UGA14 that displayed novel plasmid, pseudogene and resistance features, as well as the ability to ferment both lactose and sucrose, highlighting the co-evolution of plasmid-mediated metabolic traits and cephalosporin resistance in NTS strains. The findings emphasize the importance of combined genomic and phenotypic approaches in detecting and characterizing atypical NTS isolates to inform efforts in controlling invasive and antimicrobial resistant NTS.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Infectious Diseases
Asseta Kagambega, Elizabeth A. McMillan, Soutongnooma C. Bouda, Lari M. Hiott, Hazem Ramadan, Daniel K. Soro, Poonam Sharma, Sushim K. Gupta, Nicolas Barro, Charlene R. Jackson, Jonathan G. Frye
Summary: The emergence of antimicrobial-resistant bacteria in developing countries poses risks to both local residents and the global community. Investigating antimicrobial-resistant pathogens is essential in countries like Burkina Faso, where surveillance data is lacking. This study used whole-genome sequencing to analyze Salmonella strains isolated from slaughtered chickens in Burkina Faso, revealing the prevalence of antibiotic resistance genes and the potential for cross-contamination with chicken products. Adequate attention to safe handling methods is necessary to prevent the spread of drug-resistant pathogens.
Article
Microbiology
Laura M. Carroll, Rian Pierneef, Masenyabu Mathole, Itumeleng Matle
Summary: This study characterized 63 Salmonella enterica strains isolated from livestock, companion animals, wildlife, and animal products in South Africa using WGS, revealing diverse serotypes and sequence types, as well as multiple antibiotic-resistant subtypes. The characterization of animal-related Salmonella strains in South Africa provides critical insights into a region with a high burden of salmonellosis.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Baiyan Gong, Hong Li, Yulian Feng, Shihan Zeng, Zhenxu Zhuo, Jiajun Luo, Xiankai Chen, Xiaoyan Li
Summary: This study conducted an epidemiological investigation among hospitalized patients in Conghua District, Guangzhou, China, and found a high prevalence of NTS infection in the area, with Salmonella Typhimurium being the most common serotype. Children and those who received artificial feeding were more susceptible to infection. The majority of strains were resistant to ampicillin, with a significant percentage being multi-drug resistant.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Genetics & Heredity
Cheryll M. Sia, Sarah L. Baines, Mary Valcanis, Darren Y. J. Lee, Anders Gonsalves da Silva, Susan A. Ballard, Marion Easton, Torsten Seemann, Benjamin P. Howden, Danielle J. Ingle, Deborah A. Williamson
Summary: This study demonstrates that whole-genome sequencing (WGS) can improve antimicrobial resistance (AMR) surveillance in Non-typhoidal Salmonella (NTS) datasets, aiding in the detection of emerging AMR in NTS and specifically resistance to third generation cephalosporins (3GC).
MICROBIAL GENOMICS
(2021)
Article
Multidisciplinary Sciences
Polrat Wilairatana, Wanida Mala, Wiyada Kwanhian Klangbud, Kwuntida Uthaisar Kotepui, Pongruj Rattaprasert, Manas Kotepui
Summary: The study found that the overlap of malaria parasites and Salmonella spp. in the tropics could lead to co-infections being overestimated, with a higher association between malaria and Salmonella in children. Future longitudinal studies with appropriate diagnostic methods are recommended to accurately determine co-infection prevalence and outcomes.
SCIENTIFIC REPORTS
(2021)
Article
Veterinary Sciences
Belisa Usmael, Bruk Abraha, Sisay Alemu, Bahar Mummed, Adem Hiko, Abdallahi Abdurehman
Summary: This study reveals the importance of dogs as carriers and spreaders of antimicrobial resistant non-typhoidal Salmonella, especially in diarrheic and apparently healthy housed dogs. The risk of non-typhoidal Salmonella spread is higher in households that use offal as the main feed type. Therefore, an integrated approach is needed to tackle this problem.
BMC VETERINARY RESEARCH
(2022)
Article
Microbiology
Minori Takaichi, Kayo Osawa, Ryohei Nomoto, Noriko Nakanishi, Masanori Kameoka, Makiko Miura, Katsumi Shigemura, Shohiro Kinoshita, Koichi Kitagawa, Atsushi Uda, Takayuki Miyara, Ni Made Mertaniasih, Usman Hadi, Dadik Raharjo, Ratna Yulistiani, Masato Fujisawa, Kuntaman Kuntaman, Toshiro Shirakawa
Summary: This study confirms the increase in antibiotic resistance in non-typhoidal Salmonella enterica (NTS) in Indonesia. The NTS strains isolated from chicken meat showed high resistance to multiple antibiotics and had many virulence factors. Particularly, a serotype called S. Schwarzengrund was frequently detected as multi-antimicrobial resistant and had a high prevalence of virulence genes.
Article
Biochemistry & Molecular Biology
Ayoub Khalfaoui, Emira Noumi, Soumia Belaabed, Kaiss Aouadi, Bouslama Lamjed, Mohd Adnan, Andrea Defant, Adel Kadri, Mejdi Snoussi, Mushtaq Ahmad Khan, Ines Mancini
Summary: The study revealed that the chloroform extract from Asphodelus tenuifolius showed high antioxidant and antibacterial activities, but weak antifungal activity and no antiviral activity. The chloroform extract exhibited moderate cytotoxicity on VERO cell line, while the butanol extract was non-toxic. In addition, the main compounds from the chloroform extract demonstrated promising pharmacokinetic and drug-likeness properties.
Article
Health Care Sciences & Services
Pune N. Paque, Christopher Herz, Daniel B. Wiedemeier, Konstantinos Mitsakakis, Thomas Attin, Kai Bao, Georgios N. Belibasakis, John P. Hays, Joel S. Jenzer, Wendy E. Kaman, Michal Karpisek, Philipp Korner, Johannes R. Peham, Patrick R. Schmidlin, Thomas Thurnheer, Florian J. Wegehaupt, Nagihan Bostanci
Summary: This study identified four salivary biomarkers crucial for predicting caries accurately and developed an RF model for classification and prediction of caries progression. The high correlation between RF prediction and clinical classification was observed, indicating a new approach for caries identification.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Microbiology
Astrid P. Heikema, Rick Jansen, Saskia D. Hiltemann, John P. Hays, Andrew P. Stubbs
Summary: The ability to completely assemble plasmid sequences is crucial for studying antibiotic resistance genes and plasmid mobility. The new software pipeline 'WeFaceNano' provides a user-friendly interface for accurate plasmid assembly and the detection of antimicrobial resistance genes, overcoming challenges faced with long-read sequencing platforms. It has been validated with publicly available plasmid sequence data and is suitable for (clinical) samples with multiple plasmids.
Article
Agriculture, Dairy & Animal Science
Khyati Bhardwaj, Suchitra M. Shenoy, Shrikala Baliga, B. Unnikrishnan, B. Shantharam Baliga, Vasanth Kumar Shetty
Summary: The study conducted in poultry farms of coastal Southern Karnataka, India, found high levels of antibiotic resistance in Escherichia coli and Klebsiella pneumoniae isolates, with a focus on resistance to fluoroquinolones and tetracycline. The most common resistance genes detected were qnrB, qnrS, and qnrA, with most isolates showing high MAR indices reflecting widespread antibiotic use.
Article
Microbiology
Nikolaos Strepis, Anne F. Voor In't Holt, Margreet C. Vos, Willemien H. A. Zandijk, Astrid P. Heikema, John P. Hays, Juliette A. Severin, Corne H. W. Klaassen
Summary: The study retrospectively characterized the genetic composition and epidemiology of plasmids from mcr-1-positive bacterial isolates from a large academic hospital in the Netherlands. Results showed that mcr-1-carrying plasmids had diverse genetic backgrounds, involving different types of plasmids, and transmission events occurred within patients, between patients, and across different species.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Jayaseelan Murugaiyan, P. Anand Kumar, G. Srinivasa Rao, Katia Iskandar, Stephen Hawser, John P. Hays, Yara Mohsen, Saranya Adukkadukkam, Wireko Andrew Awuah, Ruiz Alvarez Maria Jose, Nanono Sylvia, Esther Patience Nansubuga, Bruno Tilocca, Paola Roncada, Natalia Roson-Calero, Javier Moreno-Morales, Rohul Amin, Ballamoole Krishna Kumar, Abishek Kumar, Abdul-Rahman Toufik, Thaint Nadi Zaw, Oluwatosin O. Akinwotu, Maneesh Paul Satyaseela, Maarten B. M. van Dongen
Summary: This review explores the development of new antimicrobial drugs and alternative strategies to traditional antibiotics. Various alternative approaches, including combination therapy, techniques targeting antimicrobial resistance enzymes or proteins, drug delivery systems, etc., are proposed as potential solutions.
Article
Multidisciplinary Sciences
Yunlei Li, Chantal B. van Houten, Stefan A. Boers, Ruud Jansen, Asi Cohen, Dan Engelhard, Robert Kraaij, Saskia D. Hiltemann, Jie Ju, David Fernandez, Cristian Mankoc, Eva Gonzalez, Wouter J. de Waal, Karin M. de Winter-de Groot, Tom F. W. Wolfs, Pieter Meijers, Bart Luijk, Jan Jelrik Oosterheert, Sanjay U. C. Sankatsing, Aik W. J. Bossink, Michal Stein, Adi Klein, Jalal Ashkar, Ellen Bamberger, Isaac Srugo, Majed Odeh, Yaniv Dotan, Olga Boico, Liat Etshtein, Meital Paz, Roy Navon, Tom Friedman, Einav Simon, Tanya M. Gottlieb, Ester Pri-Or, Gali Kronenfeld, Kfir Oved, Eran Eden, Andrew P. Stubbs, Louis J. Bont, John P. Hays
Summary: The study developed high-accuracy multi-parametric machine learning classifiers to differentiate bacterial from viral infections in LRTI patients of various ages. The predictive value of four easy-to-collect clinical variables was demonstrated, facilitating personalized and accurate clinical decision-making. Nasal cavity microbiota correlated with clinical variables, but may not significantly add value to diagnostic algorithms aiming to differentiate bacterial from viral infections.
Article
Microbiology
Dimard E. Foudraine, Camiel N. M. Aarents, Agnes A. Wattel, Ria van Boxtel, Nikolaos Strepis, Marian T. ten Kate, Annelies Verbon, Theo M. Luider, Corne H. W. Klaassen, John Hays, Lennard J. M. Dekker, Jan Tommassen, Wil H. F. Goessens
Summary: Extended-Spectrum beta-Lactamases (ESBL) and AmpC beta-lactamases can inhibit the activity of carbapenem antibiotics by preventing their interaction with penicillin-binding proteins. When present in high concentrations along with decreased antibiotic influx, they can confer carbapenem resistance in Enterobacterales. A targeted LC-MS/MS method was developed to detect beta-lactamases and porins in Escherichia coli, providing potential clinical value in the early detection of non-carbapenemase-producing carbapenem-resistant E. coli.
FRONTIERS IN MICROBIOLOGY
(2022)
Editorial Material
Infectious Diseases
John P. Hays, Maria Jose Ruiz-Alvarez, Natalia Roson-Calero, Rohul Amin, Jayaseelan Murugaiyan, Maarten B. M. van Dongen
Summary: This manuscript approaches the issue of increasing global antimicrobial resistance from two perspectives not usually covered by previous publications, namely the ethical use of antibiotics and potential issues in implementing new antibiotics.
INFECTIOUS DISEASES AND THERAPY
(2022)
Article
Multidisciplinary Sciences
Amogelang R. Raphenya, James Robertson, Casper Jamin, Leonardo de Oliveira Martins, Finlay Maguire, Andrew G. McArthur, John P. Hays
Summary: Whole genome sequencing is an important tool in identifying disease-associated bacteria and their antimicrobial resistance genes. However, the use of different bioinformatics software can lead to inconsistent results. Therefore, researchers need to carefully evaluate their genomic and metagenomic analysis methods using a common dataset. A reference genomic and simulated metagenomic dataset has been provided for benchmarking studies and tool development.
Article
Biochemistry & Molecular Biology
Waleed Ahmed, Ali H. Al-Marzouqi, Muhammad Hamza Nazir, Tahir A. Rizvi, Essam Zaneldin, Mushtaq Khan
Summary: Due to COVID-19 pandemic, novel strategies to combat pathogens are on the rise worldwide. This research investigates an antimicrobial polymeric PLA-based composite material enhanced with different microparticles. Results showed that the sample prepared with 90% copper and 10% PLA exhibited the best antibacterial activity, making it a sustainable solution for various applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Infectious Diseases
John Philip Hays, Kazi Sarjana Safain, Mohammed Suliman Almogbel, Ihab Habib, Mushtaq Ahmad Khan
Summary: Antimicrobial resistance (AMR) is a global problem in countries of the Arabian Peninsula, with a particular concern for the development of extended-spectrum beta-lactamases (ESBLs). The limited options for antibiotic treatment of ESBL-producing bacteria are further complicated by the development of carbapenem resistance (CR), which is commonly used to treat such infections. Recent publications indicate the presence of ESBL and/or CR in patients and hospitals across most countries of the Arabian Peninsula, with a growing emphasis on CR. Enhanced surveillance for ESBL/CR is recommended for all countries in the region.
Article
Biochemistry & Molecular Biology
Waleed Ahmed, Ali H. Al-Marzouqi, Muhammad Hamza Nazir, Tahir A. Rizvi, Essam Zaneldin, Mushtaq Khan, Muthanna Aziz
Summary: Novel strategies and materials have gained attention due to the current pandemic, high global market competition, and pathogen resistance. There is a need to develop cost-effective, eco-friendly, and biodegradable materials to combat bacteria. Fused filament fabrication (FFF), also known as fused deposition modeling (FDM), is the most effective method to develop these composites.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Shamshul Ansari, John P. Hays, Andrew Kemp, Raymond Okechukwu, Jayaseelan Murugaiyan, Mutshiene Deogratias Ekwanzala, Maria Josefina Ruiz Alvarez, Maneesh Paul-Satyaseela, Chidozie Declan Iwu, Clara Balleste-Delpierre, Ed Septimus, Lawrence Mugisha, Joseph Fadare, Susmita Chaudhuri, Vindana Chibabhai, J. M. Rohini W. W. Wadanamby, Ziad Daoud, Yonghong Xiao, Thulasiraman Parkunan, Yara Khalaf, Nkuchia M. M'Ikanatha, Maarten B. M. van Dongen
Summary: The COVID-19 pandemic poses a potential impact on global antimicrobial resistance, with critically ill patients possibly leading to overuse of antibiotics and mild cases also potentially misusing antibiotics. There is a need for increased awareness of antimicrobial resistance, proper antibiotic use, and strengthening of infection control and diagnostics.
JAC-ANTIMICROBIAL RESISTANCE
(2021)
Article
Public, Environmental & Occupational Health
Khyati Bhardwaj, Suchitra M. Shenoy, Shrikala Baliga, B. Unnikrishnan, B. Shantharam Baliga
Summary: The study revealed that most respondents have a fairly appropriate attitude towards accessing antibiotics, but there are misconceptions regarding antibiotic use and effects. Knowledge regarding antibiotic resistance and its causes is limited, highlighting the need for creating awareness among the public on the prudent use of antibiotics in the future.
CLINICAL EPIDEMIOLOGY AND GLOBAL HEALTH
(2021)
