Article
Cell Biology
Takafumi Ogawa, Koji Masumura, Yuki Kohara, Muneyoshi Kanai, Tomoyoshi Soga, Yoshikazu Ohya, T. Keith Blackwell, Masaki Mizunuma
Summary: Methionine restriction can extend lifespan and delay aging-associated pathologies. Supplementation with S-adenosyl-L-homocysteine (SAH) can mimic the effects of methionine restriction by reducing methionine levels and activating AMP-activated protein kinase (AMPK), thereby extending lifespan.
Article
Biochemistry & Molecular Biology
Mangal S. Kadam, V. L. S. Prasad Burra
Summary: Methylation plays a significant role in various diseases, and methyltransferases are important targets for drug development. However, the function of methyltransferases relies on the presence of SAM. This article provides a comprehensive analysis of SAM-receptor interactions and reveals crucial insights into the structure and function of SAM.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Review
Nutrition & Dietetics
Hammad Ullah, Ayesha Khan, Kannan R. R. Rengasamy, Alessandro Di Minno, Roberto Sacchi, Maria Daglia
Summary: Depression is a prevalent and serious health issue globally, and current treatment options are limited. SAMe and probiotics have shown potential therapeutic roles in depression by regulating microbial imbalance and reducing inflammation.
Article
Biochemistry & Molecular Biology
Ryuichi Iwasaki, Tomohiro Bito, Atsushi Ishihara, Fumio Watanabe, Yukinori Yabuta
Summary: SAM and SAH are important biochemical intermediates, with SAM being the major methyl donor in vivo. The SAM to SAH ratio is a marker of methylation capacity. Stable isotope-labeled SAM and SAH are used to measure this ratio with high sensitivity. We focused on the SAHH of Pyrococcus horikoshii OT3 and prepared recombinant P. horikoshii SAHH to efficiently produce labeled SAH.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Zhong-Ce Hu, Chui-Mu Zheng, Yun-Chao Tao, Shu-Nan Wang, Yuan-Shan Wang, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: This study aimed to increase the accumulation of S-adenosyl-L-methionine (SAM) in Saccharomyces cerevisiae by promoting ATP availability. The results showed that the lack of gene sod1 benefited ATP generation and positively regulated SAM synthesis. Additionally, improving substrate assimilation further enhanced SAM production. The SAM concentration of strain Delta SOD1 was increased from 7.3 g L-1 to 10.1 g L-1 in a 5-L fermenter.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Review
Oncology
Jiamin Guo, Yanzhong Yang, Ralf Buettner, Steven T. Rosen
Summary: This review summarizes the biological roles of methionine, methionine adenosyl transferase 2A (MAT2A), and S-adenosyl methionine (SAM) in methylation reactions during tumorigenesis. It discusses newly emerged inhibitors targeting the methionine-MAT2A-SAM axis. Recent studies have confirmed the essential roles of methionine and MAT2A in SAM biosynthesis and validated MAT2A as a metabolic dependency of cancer cells. Inhibition of MAT2A has shown synthetic lethality in MTAP-deleted cancers. Notably, significant progress has been made in developing inhibitors targeting the methionine-MAT2A-SAM axis, with first-in-class MAT2A inhibitors entering clinical trials. The methionine-MAT2A-SAM axis plays a critical role in tumorigenesis through providing SAM for abnormal protein, DNA, and RNA methylation in cancer cells. Targeting SAM biosynthesis through MAT2A inhibition has emerged as a novel and promising strategy for cancer therapy.
CURRENT OPINION IN ONCOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Siyu Ren, Xinkuan Cheng, Long Ma
Summary: Natural fluorinated products are rare and of great interest. The biosynthetic pathway of de novo fluorometabolites in microbes has been studied, revealing the key roles of fluorinase (FLA) and a novel methionine adenosyltransferase from Streptomyces xinghaiensis (SxMAT) in the synthesis of 5'-fluoro-deoxy-adenosine.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Biochemical Research Methods
Kaixing Xiao, Qian Kang, Mengjie Xiang, Dachun Gong, Huan Fang, Xuan Tu, Dawei Zhang
Summary: This study reports a cell-free synthetic system for producing hydrogenobyrinic acid by integrating 12 enzymes using 5-aminolevulininate as a substrate. It also develops a novel S-adenosyl-methionine regeneration system to steadily supply S-adenosyl-methionine and avoid the accumulated inhibition of S-adenosyl-homocysteine.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Chang Dong, J. Carl Schultz, Wei Liu, Jiazhang Lian, Lei Huang, Zhinan Xu, Huimin Zhao
Summary: This study identified novel targets for enhancing SAM production through genome-scale engineering (GSE) and validated their effects in industrial yeast strains, leading to significant increases in SAM productivity. Transcriptomic and metabolomic analyses were conducted to investigate the molecular mechanisms for enhanced SAM accumulation.
METABOLIC ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Jun-Min Lee, Min-Ho Park, Bu-Soo Park, Min-Kyu Oh
Summary: This study achieved microbial production of S-methyl-methionine (SMM) by introducing a plant-derived MMT gene into engineered Saccharomyces cerevisiae sake K6.
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Hailong Chen, Xiaoqin Chai, Yan Wang, Jing Liu, Guohai Zhou, Pinghe Wei, Yuhe Song, Lingman Ma
Summary: Background: Saccharomyces cerevisiae is commonly used for the production of S-adenosyl-l-methionine (SAM), but glucose repression negatively affects its production. This study shows that deleting REG1 and overexpressing SNF1 can improve SAM production in S. cerevisiae by enhancing glucose utilization and glycolysis, promoting ethanol conversion, enhancing precursor amino acid biosynthesis, and extending yeast lifespan.
MICROBIAL CELL FACTORIES
(2022)
Article
Microbiology
Rebeca Dominguez-Santos, Katarina Kosalkova, Isabel-Clara Sanchez-Orejas, Carlos Barreiro, Yolanda Perez-Pertejo, Rosa M. Reguera, Rafael Balana-Fouce, Carlos Garcia-Estrada
Summary: This article describes the use of the filamentous fungus Penicillium chrysogenum in the industrial production of penicillin and highlights the role of AdoMet synthetase in both primary and secondary metabolism in this process.
Article
Cell Biology
Renata Orlowska, Jacek Zebrowski, Janusz Zimny, Piotr Androsiuk, Piotr Tomasz Bednarek
Summary: This study used a structural equation modeling approach to analyze the effects of Cu(II) and Ag(I) ions, DNA methylation, and S-adenosyl-L-methionine on the regeneration efficiency of triticale through in vitro culture. The results revealed the relationships between these factors and provided a theoretical model for the biological phenomena associated with green plant regeneration efficiency.
Article
Biochemistry & Molecular Biology
Aida Corrillero Bravo, Maria Nieves Ligero Aguilera, Nahuel R. Marziali, Lennart Moritz, Victoria Wingert, Katharina Klotz, Anke Schumann, Sarah C. Gruenert, Ute Spiekerkoetter, Urs Berger, Ann-Kathrin Lederer, Roman Huber, Luciana Hannibal
Summary: S-adenosylmethionine (SAM) is essential for methyl transfer reactions. SAM is produced de novo via the methionine cycle. The demethylation of SAM produces S-adenosylhomocysteine (SAH), an inhibitor of methyltransferases. Measurement of SAM and SAH in plasma is valuable for diagnosing inborn errors of metabolism and assessing methyl group homeostasis.
Article
Cell Biology
Xinquan Zhang, Wen Meng, Jian Feng, Xinghong Gao, Chao Qin, Pinghui Feng, Yufei Huang, Shou-Jiang Gao
Summary: This study identifies the suppressive role of RNA methyltransferase METTL16 in KSHV lytic replication. METTL16 regulates the SAM cycle to maintain intracellular SAM level and redox homeostasis, thereby inhibiting KSHV lytic replication.
CELL DEATH & DISEASE
(2023)
Review
Pharmacology & Pharmacy
Akash Saggam, Kirti Limgaokar, Swapnil Borse, Preeti Chavan-Gautam, Santosh Dixit, Girish Tillu, Bhushan Patwardhan
Summary: This review highlights the potential of Ashwagandha in managing COVID-19, showcasing its immunomodulatory, anti-inflammatory, and organ protective properties. It proposes Ashwagandha as a therapeutic adjuvant for COVID-19 management and suggests the need for further systematic studies to fully realize its potential.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Pediatrics
Sarika Chaturvedi, Girish Tillu, Anil Kale, Aruna Pendse, Ashwini Kulkarni, Deepali Ambike, Madhumita Krishnan, Manoj Gaikwad, Monica Mulay, Murlidhar Prabhudesai, Nilangi Nanal, Neelima Tillu, Pramod Jog, Samina Jamadar, Sandeep Kadam, Sarvesh Kumar Singh, Satyalakshmi Komarajju, Sharad Agarkhedkar, Sudhir Malwade, Bhushan Patwardhan
Summary: This paper presents a standardized protocol for infant massage and describes a feasible method to develop an integrative protocol involving traditional and modern medicine experts through a series of Delphi studies. The current protocol for infants born beyond 34 weeks of gestation and weighing above 1.8 kg is recommended, providing detailed guidance on massage preparation and procedures.
JOURNAL OF TROPICAL PEDIATRICS
(2021)
Editorial Material
Medicine, General & Internal
Yamini Aiyar, Vijay Chandru, Mirai Chatterjee, Sapna Desai, Armida Fernandez, Atul Gupta, Gagandeep Kang, Tarun Khanna, Kiran Mazumdar-Shaw, Nachiket Mor, Arnab Mukherji, Poonam Muttreja, Thelma Narayan, Bhushan Patwardhan, K. Sujatha Rao, Sharad Sharma, Devi Shetty, S. V. Subramanian, Leila E. Caleb Varkey, Sandhya Venkateswaran, Vikram Patel
Editorial Material
Neurosciences
Akshay Anand, Bhushan Patwardhan
ANNALS OF NEUROSCIENCES
(2021)
Article
Integrative & Complementary Medicine
Arvind Chopra, Narayanam Srikanth, Bhushan Patwardhan, Ashwinikumar Raut, Manohar Gundeti, Muffazal Lakdawala, Govind Reddy, Dilip Gode, Kuldeep Chaudhary, Prasanna Kumar Rao, Shruti Khanduri, Sanjay Tamboli, Manjit Saluja, Girish Tillu
Summary: The study aimed to compare the efficacy and safety of Withania somnifera with hydroxychloroquine in the prophylaxis against COVID-19 in high-risk healthcare workers. The results showed that Ashwagandha was not inferior to hydroxychloroquine in terms of COVID-19 prevention and also had additional clinical benefits.
COMPLEMENTARY THERAPIES IN MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Akash Saggam, Prathamesh Kale, Sushant Shengule, Dada Patil, Manish Gautam, Girish Tillu, Kalpana Joshi, Sunil Gairola, Bhushan Patwardhan
Summary: The study successfully alleviated paclitaxel-induced myelosuppression by using extracts of Asparagus racemosus and Withania somnifera, and modulated cytokines to avoid paclitaxel-induced leukopenia, neutropenia, and associated symptoms.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Medicine, General & Internal
Arvind Chopra, Preeti Chavan-Gautam, Girish Tillu, Manjit Saluja, Swapnil Borse, Sanjeev Sarmukaddam, Susmita Chaudhuri, B. C. S. Rao, Babita Yadav, Narayanam Srikanth, Bhushan Patwardhan
Summary: Vaccines are crucial in combating COVID-19, and this study focuses on investigating the safety, immunogenicity, and clinical protection offered by Ashwagandha in individuals vaccinated with COVISHIELD™ in India.
FRONTIERS IN MEDICINE
(2022)
Editorial Material
Integrative & Complementary Medicine
Bhushan Patwardhan, Balasubramani S. Paranthaman
JOURNAL OF AYURVEDA AND INTEGRATIVE MEDICINE
(2021)
Correction
Medicine, General & Internal
Arvind Chopra, Preeti Chavan-Gautam, Girish Tillu, Manjit Saluja, Swapnil Borse, Sanjeev Sarmukaddam, Susmita Chaudhuri, B. C. S. Rao, Babita Yadav, Narayanam Srikanth, Bhushan Patwardhan
FRONTIERS IN MEDICINE
(2022)
Editorial Material
Integrative & Complementary Medicine
Kalpana Joshi, Bhushan Patwardhan, M. S. Valiathan
JOURNAL OF AYURVEDA AND INTEGRATIVE MEDICINE
(2023)
Editorial Material
Pharmacology & Pharmacy
Bhushan Patwardhan
EXPERT OPINION ON DRUG DISCOVERY
(2023)
Article
Multidisciplinary Sciences
Arvind Chopra, Girish Tillu, Kuldeep Chuadhary, Govind Reddy, Alok Srivastava, Muffazal Lakdawala, Dilip Gode, Himanshu Reddy, Sanjay Tamboli, Manjit Saluja, Sanjeev Sarmukaddam, Manohar Gundeti, Ashwini Kumar Raut, B. C. S. Rao, Babita Yadav, Narayanam Srikanth, Bhushan Patwardhan
Summary: The efficacy of AYUSH 64, a standard polyherbal Ayurvedic drug, in COVID-19 was evaluated in this study. The results showed that the combination of AYUSH 64 with standard of care can expedite recovery, reduce hospitalization, and improve health in COVID-19 patients. However, further clinical validation (Phase III) is required.
Article
Health Care Sciences & Services
Sarika Chaturvedi, John Porter, Geetha Krishnan Gopalakrishna Pillai, Leena Abraham, Darshan Shankar, Bhushan Patwardhan
Summary: This article provides insights into using traditional medicine systems for Universal Health Coverage (UHC). It emphasizes the need to reimagine India's health system and adopt an inclusive approach for UHC. The challenges with appropriate use of traditional medicine systems and lessons from international experience are discussed, along with pathways for better utilization of traditional medicine systems for UHC in India.
LANCET REGIONAL HEALTH - SOUTHEAST ASIA
(2023)
Review
Integrative & Complementary Medicine
Indranill Basu-Ray, Kashinath Metri, Dibbendhu Khanra, Rishab Revankar, Kavitha M. Chinnaiyan, Nagaratna Raghuram, Mahesh Chandra Mishra, Bhushan Patwardhan, Manjunath Sharma, Ishwar Basavaraddi, Akshay Anand, Shrinath Reddy, K. K. Deepak, Marian Levy, Sue Theus, Glenn N. Levine, Holger Cramer, Gregory L. Fricchione, Nagendra R. Hongasandra
Summary: This review article explores the potential of yoga in enhancing innate immunity and improving mental health, as well as its role in the prevention and adjunctive treatment of COVID-19.
BMC COMPLEMENTARY MEDICINE AND THERAPIES
(2022)
Letter
Multidisciplinary Sciences
Bhushan Patwardhan, Ravindra Ghooi, Sharad Deshpande