Article
Parasitology
Jian Li, Jing Ding, Xiao-Lei Liu, Bin Tang, Xue Bai, Yang Wang, Wei-Dong Qiao, Ming-Yuan Liu, Xue-Lin Wang
Summary: This study successfully developed a rapid and sensitive diagnostic method named UPT-LF-ES assay for detecting anti-T. spiralis IgG antibodies in pig serum, based on UCNPs-ES/goat anti-rabbit IgG probes. The assay showed high sensitivity and specificity, contributing to preventing human trichinellosis effectively.
PARASITES & VECTORS
(2021)
Article
Parasitology
Sylwia Grzelak, Anna Stachyra, Justyna Bien-Kalinowska
Summary: This study aimed to identify common and species-specific excretory-secretory proteins of T. spiralis and T. britovi adult worms for early diagnosis of Trichinella infection. Through various analyses, several key proteins were identified that could potentially be used for diagnostic purposes.
VETERINARY PARASITOLOGY
(2021)
Article
Parasitology
Lingqin Wu, Wenhui Yin, Jutai Wen, Shuying Wang, Huihui Li, Xiaoli Wang, Weixiao Zhang, Shuyao Duan, Qiuyu Zhu, Erhe Gao, Shili Wu, Bin Zhan, Rui Zhou, Xiaodi Yang
Summary: The study showed that treatment with Ts-AES significantly improved cardiac function and ventricular remodeling, reduced pathological damage and mortality in mice with MI. This was associated with decreased pro-inflammatory cytokine levels, increased regulatory cytokine expression, and promotion of macrophage polarization from M1 to M2 type in MI mice.
PARASITES & VECTORS
(2023)
Article
Veterinary Sciences
Xinyu Wang, Bin Tang, Ying Zhao, Jing Ding, Nan Wang, Yan Liu, Zijian Dong, Xiangdong Sun, Quangang Xu, Mingyuan Liu, Xiaolei Liu
Summary: A rapid and sensitive immunochromatographic strip based on EuNPs was developed for the detection of Trichinella spiralis infection in pigs, providing earlier detection of antibodies compared to traditional methods.
VETERINARY RESEARCH
(2021)
Article
Immunology
Jing -Bo Zhen, Jin-Peng Zhang, Feng Sun, Li-Hao Lin, Yu-Heng Zhang, Rui-Biao Wang, Yang Han, Zhi-Xin Li, Yi-Xin Lu
Summary: Allergic asthma, a chronic, heterogeneous, and inflammatory respiratory disease, has limited treatment options. This study focused on the effects of Trichinella spiralis (T. spiralis) excretory-secretory (ES) antigens on allergic asthma. The study used Ts43, Ts49, and Ts53 proteins, important components of ES antigens, to establish ES antigens intervention models in mice. The results showed that ES antigens could alleviate asthma symptoms, weight loss, and lung inflammation, with better effects observed with combined intervention of Ts43, Ts49, and Ts53. Furthermore, the study investigated the changes in Th1 and Th2 immune responses and the differentiation direction of T lymphocytes in mice. The results indicated a decrease in the ratio of CD4+/CD8+ T cells and an increase in the ratio of Th1/Th2 cells. Overall, this study demonstrated that T. spiralis ES antigens could attenuate allergic asthma in mice by altering the differentiation of CD4+ and CD8+ T cells and regulating the Th1/Th2 cell ratio.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Parasitology
Jing Wang, Bin Tang, Xihuo You, Xuepeng Cai, Wanzhong Jia, Xiaolei Liu, Mingyuan Liu, Xuemin Jin, Jing Ding
Summary: This study investigated the effects of Trichinella spiralis' excretory/secretory products (ESP) on the functions of immune cells. The results revealed the evasion and regulation mechanisms employed by the parasite, providing insights into its strategies to manipulate host immunity.
PARASITES & VECTORS
(2023)
Article
Cell Biology
Ana L. Rios-Lopez, Romel Hernandez-Bello, Gloria M. Gonzalez, Alejandro Sanchez-Gonzalez
Summary: The study reveals that Trichinella spiralis excretory-secretory (ES) antigens inhibit the release of NETs by neutrophils, while other antimicrobial functions remain unchanged. This may offer a novel strategy for treating autoimmune pathologies involving NETs formation.
CELLULAR IMMUNOLOGY
(2022)
Article
Immunology
Huihui Li, Dapeng Qiu, Huijuan Yang, Yuan Yuan, Lingqin Wu, Liang Chu, Bin Zhan, Xiaoli Wang, Yan Sun, Wei Xu, Xiaodi Yang
Summary: Ts-AES has a therapeutic effect on sepsis-induced ALI by inhibiting pro-inflammatory cytokines and activating Tregs response. The expression of HMGB1, TLR2, and MyD88 in lung tissue was inhibited after treatment with Ts-AES. Ts-AES may exert immunomodulatory functions through the HMGB1/TLR2/MyD88 signaling pathway.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Parasitology
Fabrizio Bruschi, Alisa Gruden-Movesijan, Barbara Pinto, Natasa Ilic, Ljiljana Sofronic-Milosavljevic
Summary: This study investigated the effects of products extracted from Trichinella spiralis muscle larvae on the experimental autoimmune encephalomyelitis (EAE) rat model, finding that treatment with ES L1 could alleviate EAE symptoms. Furthermore, lower levels of serum MMP-9 and NGAL/MMP-9 complex were observed in rats treated with ES L1, indicating a potential mechanism for the amelioration of EAE.
EXPERIMENTAL PARASITOLOGY
(2021)
Review
Microbiology
Bin Tang, Jian Li, Tingting Li, Yiting Xie, Wei Guan, Yanqing Zhao, Shuguo Yang, Mingyuan Liu, Daoxiu Xu
Summary: This article provides an overview of the strategies for developing effective vaccines against Trichinella spiralis and summarizes the factors that influence vaccine effectiveness. The study suggests that the protective effect against T. spiralis infection can be improved by selecting suitable antigens and using different types of vaccines. However, there is still limited research on T. spiralis vaccines in pigs, and more studies are needed to explore the protective effect in swine models.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Xinyu Wang, Aizhe Li, Ruizhe Wang, Tianji Hou, Huixin Chen, Jing Wang, Mingyuan Liu, Chen Li, Jing Ding
Summary: Trichinellosis is a food-borne parasitic infection caused by consuming raw or semi-raw pork products infected with Trichinella spiralis. A rapid and sensitive on-site diagnostic method using a novel lateral flow immunoassay strip (ICS) was developed to detect T. spiralis-specific IgG antibody in pig serum and whole blood samples. The method was successfully used to investigate Trichinella infection in five provinces of China, and the results correlated with the standard method of artificial digestion.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Chengyao Li, Xue Bai, Xiaolei Liu, Yuanyuan Zhang, Lei Liu, Lixiao Zhang, Fengyan Xu, Yong Yang, Mingyuan Liu
Summary: Through an in vitro cell monolayer model, it was found that the excretory secretory products of Trichinella spiralis muscle larvae can disrupt the host intestinal barrier by reducing the expression of tight junction proteins. This effect is likely mediated via the MAPK signaling pathway, highlighting serine protease as a key factor for invasion by T. spiralis.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Parasitology
Utsanee Supcharoengoon, Onrapak Reamtong, Paron Dekumyoy, Dorn Watthanakulpanich, Yanin Limpanont, Lv Zhiyue, Salisa Chaimon, Pongsakorn Martviset, Poom Adisakwattana
Summary: This study identified specific antigens with molecular weights of 43, 79, and 101 kDa in crude somatic antigens (CSAg) of Trichinella spiralis muscle larvae (TsML). These antigens showed high sensitivity and specificity in IgG-ELISA detection. LC-MS-MS analysis identified 11 different proteins in these antigens, with TsCSAg-43 showing the highest specificity. Further studies should focus on the development and production of these specific proteins for immunodiagnosis.
Article
Veterinary Sciences
Haoxuan Wang, Yingying Zhu, Meichen Li, Jingdan Pan, Dan Li, Wen-Ping Guo, Guangcheng Xie, Luanying Du
Summary: The expression of 2,860 genes in A549 cells was altered by ML-ESPs, with 1,634 upregulated and 1,226 downregulated. GO and KEGG analysis revealed that the differentially expressed genes were mainly associated with cancer pathways and metabolic processes. Among the downregulated genes in the metabolic process interaction network, genes involved in glucose metabolism such as PFKM, PFKL, ENO2, LDHB, 6PGL, PRE, TKT, and TALDO1 were suppressed by ML-ESPs, while TCA-related genes including PDP1, PDP2, ACO1, and OGDH were upregulated.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Biology
Radu Pavel, Sorin Ursoniu, Maria Alina Lupu, Tudor Rares Olariu
Summary: This observational study compared the epidemiological, laboratory, clinical, and therapeutic aspects of trichinellosis in children and adults in Western Romania. The results showed a declining trend in infection rates for both children and adults during the studied period. Pork meat products were the main source of infection. Improving education and implementing public health strategies are crucial for the prevention of trichinellosis in Western Romania.