Article
Multidisciplinary Sciences
Leanna M. Owen, Nicolas A. Bax, William I. Weis, Alexander R. Dunn
Summary: This study found that talin ABS3 forms long-lived catch bonds with F-actin under force, which is necessary for normal focal adhesion formation. The results support a mechanism in which talin ABS3 preferentially binds to and orients actin filaments, leading to long-range order in the actin cytoskeleton. Talin ABS3 may serve as a molecular AND gate that allows focal adhesion growth only when sufficient integrin density, F-actin polarization, and mechanical tension are simultaneously present.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Cell Biology
Michael Bachmann, Baihao Su, Rolle Rahikainen, Vesa P. Hytonen, Jinhua Wu, Bernhard Wehrle-Haller
Summary: Talin is a cytoskeletal adapter protein that plays a key role in the formation and regulation of integrin-dependent cell-matrix adhesions. It links integrins to the actin cytoskeleton and is involved in mechanosignaling at the plasma membrane-cytoskeleton interface.
JOURNAL OF CELL SCIENCE
(2023)
Article
Cell Biology
Manos Mavrakis, M. Angeles Juanes
Summary: Cell movement is influenced by the turnover of focal adhesions, which traditionally was believed to be triggered by microtubules. However, recent research has discovered other molecular players that affect actin cytoskeleton dynamics, enabling directed cell migration.
CURRENT OPINION IN CELL BIOLOGY
(2023)
Article
Physics, Multidisciplinary
David B. Brueckner, Matthew Schmitt, Alexandra Fink, Georg Ladurner, Johannes Flommersfeld, Nicolas Arlt, Edouard Hannezo, Joachim O. Raedler, Chase P. Broedersz
Summary: This research develops a model for protrusion and polarity dynamics in confined cell migration using data-driven inference and a mechanistic bottom-up approach. The study reveals that cellular dynamics adapt to confining geometries through a switch in polarity dynamics, driving the migration of the cell through constrictions.
Review
Immunology
Chiara Cassioli, Cosima T. Baldari
Summary: Interactions among immune cells play a crucial role in adaptive immune responses. The immunological synapse (IS) serves as a specialized platform for intercellular communication between T lymphocytes and antigen presenting cells (APCs). Recent studies have shown that during early stages of IS formation, there is a depletion of centrosomal filamentous actin (F-actin), allowing for centrosome detachment from the nucleus and its polarization towards the synaptic membrane. In CD4(+) T cells, the reduction in centrosomal F-actin is dependent on the activity of a centrosome-associated proteasome, and the ciliopathy-related Bardet-Biedl syndrome 1 protein is implicated in the dynein-dependent recruitment of the proteasome 19S regulatory subunit to the centrosome.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biology
Erumbi S. S. Rangarajan, Emmanuel W. W. Smith, Tina Izard
Summary: Cell attachment is crucial and mediated by cadherin receptors binding and alpha-catenin interaction. Alpha-catenin modulates cell migration and adhesion by linking junctions and plasma membrane to actin cytoskeleton. CryoEM structures provide mechanistic insights into alpha-catenin function and its role in cell adhesion and disease.
COMMUNICATIONS BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Duygu Sari-Ak, Alvaro Torres-Gomez, Yavuz-Furkan Yazicioglu, Anthos Christofides, Nikolaos Patsoukis, Esther M. Lafuente, Vassiliki A. Boussiotis
Summary: This review discusses the structural and molecular properties of RIAM and the functional role of the Rap1/RIAM module in hematopoietic cells.
BIOMEDICAL JOURNAL
(2022)
Article
Biology
Ji-Tong Li, Xiao-Ning Cheng, Chong Zhang, De-Li Shi, Ming Shao
Summary: Cell adhesion and active cell shape changes are crucial for morphogenetic movements during embryonic development. The adaptor protein Lurap1 plays a role in regulating cell adhesion during epiboly in zebrafish embryos, contributing to coordinated morphogenetic movements.
Article
Chemistry, Multidisciplinary
Paul Mollenkopf, Dusan Prascevic, Thomas M. Bayerl, Josef A. Kaes, Joerg Schnauss
Summary: Heavy water, or deuterium oxide (D2O), has significant effects on various biological systems, particularly at the cellular level. It inhibits dynamic processes such as migration and invasion, as well as central processes of cell proliferation. D2O treatment also reduces individual cell deformabilities. Researchers discovered that D2O induces bundling in reconstituted entangled networks of filamentous actin, a novel and previously undescribed actin bundling mechanism.
Article
Multidisciplinary Sciences
Julian Knerr, Ralf Werner, Carsten Schwan, Hong Wang, Peter Gebhardt, Helga Groetsch, Almuth Caliebe, Malte Spielmann, Paul-Martin Holterhus, Robert Grosse, Nadine C. Hornig
Summary: Steroid hormone receptors are crucial for mammalian physiology and are involved in various conditions. Functional mutations in DAAM2 gene were found in patients with androgen insensitivity syndrome. DAAM2 is enriched in the nucleus and forms actin-dependent transcriptional droplets with AR in response to dihydrotestosterone, promoting gene expression and cellular functions. Our study reveals the signal-regulated nuclear actin assembly at a steroid hormone receptor, which is necessary for transcription.
Article
Cell Biology
Luis Daniel Rios-Barrera, Maria Leptin
Summary: Membrane trafficking plays a crucial role in morphogenesis. This study reveals a new role for late endosomes in directing morphogenesis by organizing actin.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Cell Biology
Nannan Gao, Andjela Raduka, Fariba Rezaee
Summary: Respiratory syncytial virus (RSV) infection, the leading cause of acute lower respiratory tract infection in young children worldwide, disrupts the airway epithelial barrier by attenuating cortactin expression and destabilizing the F-actin network. The identified pathways may serve as new targets for therapeutic intervention against RSV-related disease.
JOURNAL OF CELL SCIENCE
(2022)
Article
Biology
Campbell D. Lawson, Samantha Peel, Asier Jayo, Adam Corrigan, Preeti Iyer, Mabel Baxter Dalrymple, Richard J. Marsh, Susan Cox, Isabel Van Audenhove, Jan Gettemans, Maddy Parsons
Summary: Fascin is an important regulator of F-actin bundling in tumor cells. It has been found that Fascin bundles F-actin within the nucleus, supporting chromatin organization and efficient DNA damage response. Increased nuclear accumulation of Fascin leads to sustained nuclear F-actin bundling, resulting in reduced invasion, viability, and apoptosis of cancer cells.
Review
Immunology
Maksim V. Baranov, Manoj Kumar, Stefano Sacanna, Shashi Thutupalli, Geert van den Bogaart
Summary: The size, shape, rigidity, and surface roughness of pathogens are important parameters affecting cellular uptake and immune responses, particularly in inflammasome activation and T cell activation. Understanding how the physical properties of particles influence immune responses can assist in the design of more effective vaccines.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Orthopedics
Jie Liu, Li Yang, Hao Zhang, Jing-yu Zhang, Yong-cheng Hu
Summary: This study explored the effects of three different configurations of allogenic bone substitutes on the viability, adhesion, and spreading of osteoblasts. The results showed that bone powder and bone granule groups were more effective in promoting cell adhesion and spreading compared to the bone fiber group.
ORTHOPAEDIC SURGERY
(2023)
Article
Hematology
Maximilian Mauler, Nancy Schanze, Krystin Krauel, Claudia Schoenichen, Franziska Glatzki, Susanna Poeschl, Daniela Stallmann, Julius Mezger, Nadine Gauchel, Diana Sharipova, Marina Rieder, Ingo Hilgendorf, Thilo Witsch, Christoph Bode, Daniel Duerschmied
Summary: Peripheral serotonin seems to have no effect on endothelial adhesion molecule expression, despite its influence on neutrophil function. The inflammatory release of peripheral serotonin appears to be unnecessary for regulating endothelial adhesion molecules.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Cardiac & Cardiovascular Systems
Nadine Gauchel, Marina Rieder, Krystin Krauel, Isabella Goller, Maren Jeserich, Ulrich Salzer, Ana Cecilia Venhoff, Niklas Baldus, Luisa Pollmeier, Luisa Wirth, Winfried Kern, Siegbert Rieg, Hans-Jorg Busch, Maike Hofmann, Christoph Bode, Daniel Duerschmied, Achim Lother, Lukas A. Heger
Summary: This study compared the levels of complement system components in COVID-19 patients and other groups, revealing that COVID-19 patients had significantly elevated levels of CS components 5a and 4, increased levels of vWF antigen, and a significant correlation with vWF antigen, suggesting a pronounced immune inflammatory response in COVID-19 disease.
JOURNAL OF THROMBOSIS AND THROMBOLYSIS
(2022)
Article
Cardiac & Cardiovascular Systems
Diana A. Gorog, Robert F. Storey, Paul A. Gurbel, Udaya S. Tantry, Jeffrey S. Berger, Mark Y. Chan, Daniel Duerschmied, Susan S. Smyth, William A. E. Parker, Ramzi A. Ajjan, Gemma Vilahur, Lina Badimon, Jurrien M. ten Berg, Hugo ten Cate, Flora Peyvandi, Taia T. Wang, Richard C. Becker
Summary: COVID-19 predisposes patients to thrombotic and thromboembolic events. Numerous biomarkers have emerged to assess the thrombotic risk in these patients, informing prognosis and predicting thrombosis.
NATURE REVIEWS CARDIOLOGY
(2022)
Article
Critical Care Medicine
Alexander Supady, Timm Zahn, Moritz Kuhl, Sven Maier, Christoph Benk, Klaus Kaier, Bernd W. Boettiger, Christoph Bode, Achim Lother, Dawid L. Staudacher, Tobias Wengenmayer, Daniel Duerschmied
Summary: Cytokine adsorption did not reduce serum IL-6 levels and had no significant effect on survival, vasopressor support, or biomarkers of injury in patients receiving ECPR.
Review
Hematology
Nancy Schanze, Muataz Ali Hamad, Thomas Georg Nuehrenberg, Christoph Bode, Daniel Duerschmied
Summary: Coronary artery disease is a leading cause of global mortality, with ischemia/reperfusion injury (IRI) contributing to cardiac damage. Platelets play an important role in IRI through activation and interaction with other cells. Understanding platelet contributions to IRI is crucial for developing effective treatment strategies.
Article
Hematology
Stefano Barco, Davide Voci, Ulrike Held, Tim Sebastian, Roland Bingisser, Giuseppe Colucci, Daniel Duerschmied, Andre Frenk, Bernhard Gerber, Andrea Goetschi, Stavros Konstantinides, Francois Mach, Helia Robert-Ebadi, Thomas Rosemann, Noemi R. Simon, Herve Spechbach, David Spirk, Stefan Stortecky, Lukas Vaisnora, Marc Righini, Nils Kucher
Summary: This study aimed to investigate whether thromboprophylaxis with enoxaparin would prevent hospitalization and death in symptomatic COVID-19 outpatients. The results showed that enoxaparin did not reduce the risk of hospitalization and death among these patients.
LANCET HAEMATOLOGY
(2022)
Article
Medicine, General & Internal
Diona Gjermeni, Hannah Vetter, Sofia Szabo, Viktoria Anfang, Stefan Leggewie, David Hesselbarth, Daniel Duerschmied, Dietmar Trenk, Christoph B. Olivier
Summary: This study compared the performance of multiple platelet aggregometry (MEA) and thrombelastography (TEG) in detecting high on-clopidogrel platelet reactivity (HPR) in patients with atrial fibrillation (AF) and indication for oral anticoagulation (OAC) after percutaneous coronary intervention (PCI). The results showed that TEG detected higher rates of HPR compared to MEA. Conventional cut-off values for HPR may need to be re-evaluated for this high-risk population. Further studies are needed to assess the association with outcomes.
JOURNAL OF CLINICAL MEDICINE
(2022)
Meeting Abstract
Cardiac & Cardiovascular Systems
Diona Gjermeni, Sofia Szabo, Viktoria Anfang, Hannah Theresa Vetter, Carina Juelch, Stefan Leggewie, David Hesselbarth, Dietmar Trenk, Daniel Duerschmied, Christoph B. Olivier
CARDIOVASCULAR RESEARCH
(2022)
Article
Cardiac & Cardiovascular Systems
Christian Fastner, Goekhan Yuecel, Svetlana Hetjens, Boris Rudic, Gereon Schmiel, Matthias Toepel, Volker Liebe, Mathieu Kruska, Martin Borggrefe, Daniel Burkhoff, Ibrahim Akin, Daniel Duerschmied, Juergen Kuschyk
Summary: This study compared the effects of CCM treatment over 5 years in patients with baseline NYHA class II vs. III/IV, finding that patients with NYHA III/IV showed more significant improvements in functional parameters and survival benefits.
CLINICAL RESEARCH IN CARDIOLOGY
(2022)
Article
Peripheral Vascular Disease
Guru Prasad Sharma, Ramoji Kosuru, Sribalaji Lakshmikanthan, Shikan Zheng, Yao Chen, Robert Burns, Gang Xin, Weiguo Cui, Magdalena Chrzanowska
Summary: This study identifies the role of Rap1B in tumor growth and immunosuppression. Rap1B deficiency restricts tumor growth and enhances the activity of CD8(+) T-cells, improving the efficacy of immunotherapy.
Article
Cardiac & Cardiovascular Systems
Anna C. Mavromanoli, Stefano Barco, Walter Ageno, Helene Bouvaist, Marianne Brodmann, Claudio Cuccia, Francis Couturaud, Claudia Dellas, Konstantinos Dimopoulos, Daniel Duerschmied, Klaus Empen, Pompilio Faggiano, Emile Ferrari, Nazzareno Galie, Marcello Galvani, Alexandre Ghuysen, George Giannakoulas, Menno Huisman, David Jimenez, Matija Kozak, Irene M. Lang, Nicolas Meneveau, Thomas Muenzel, Massimiliano Palazzini, Antoniu Octavian Petris, Giancarlo Piovaccari, Aldo Salvi, Sebastian Schellong, Kai-Helge Schmidt, Franck Verschuren, Irene Schmidtmann, Gerrit Toenges, Frederikus A. Klok, Stavros Konstantinides
Summary: This study analyzed the changes in RV function in patients with intermediate-risk PE who were switched early to oral anticoagulation. The results showed that the majority of patients had normalized RV function within 6 days and maintained normal RV function throughout the first 6 months. However, almost one in four patients continued to have evidence of RV dysfunction over the long term.
CLINICAL RESEARCH IN CARDIOLOGY
(2023)
Article
Hematology
Davide Voci, Andrea Gotschi, Ulrike Held, Roland Bingisser, Giuseppe Colucci, Daniel Duerschmied, Riccardo M. Fumagalli, Bernhard Gerber, Barbara Hasse, Dagmar I. Keller, Stavros V. Konstantinides, Francois Mach, Silvana K. Rampini, Marc Righini, Helia Robert-Ebadi, Thomas Rosemann, Stephanie Roth-Zetzsche, Tim Sebastian, Noemi R. Simon, David Spirk, Stefan Stortecky, Lukas Vaisnora, Nils Kucher, Stefano Barco
Summary: The benefits of early thromboprophylaxis in symptomatic COVID-19 outpatients remain unclear. This study presents the 90-day results from the OVID phase III trial, which showed that early thromboprophylaxis with enoxaparin did not improve the course of COVID-19 in terms of hospitalization, death, or resolution of symptoms.
THROMBOSIS RESEARCH
(2023)
Review
Virology
Aydin Huseynov, Ibrahim Akin, Daniel Duerschmied, Ruediger E. Scharf
Summary: An increasing number of post-COVID patients are experiencing long-term complications from the acute COVID-19 infection, posing ongoing challenges for healthcare professionals. It has become clear that COVID-19 not only affects the respiratory system but also other organs, including the cardiovascular system. Cardiopulmonary symptoms such as shortness of breath, palpitations, limited physical capacity, and cardiac arrhythmias can persist in a significant proportion of recovered patients. This article reviews the prevalence and underlying mechanisms of COVID-19-associated arrhythmias and discusses the clinical management of various rhythm disorders in post-COVID patients.
Article
Biology
Jiro Sakai, Jiyeon Yang, Chao-Kai Chou, Wells W. Wu, Mustafa Akkoyunlu
Summary: Newborns have a lower humoral immune response than adults due to the immunoregulatory role of IL-10. This study found that both B-1 cells and non-B-1 cells in neonatal mice produce IL-10 in response to B cell antigen receptor (BCR) activation. The production of IL-10 is mediated by the activation of STAT5 and IL-6, and further activates STAT3. IL-10 secreted by neonatal non-B-1 cells can inhibit TNF-alpha secretion by macrophages.
Article
Hematology
Marina Rieder, Niklas Baldus, Daniela Stallmann, Maren Jeserich, Isabella Goller, Luisa Wirth, Luisa Pollmeier, Maike Hofmann, Christoph Bode, Hans-Joerg Busch, Bonaventura Schmid, Nadine Gauchel, Rudiger E. Scharf, Daniel Duerschmied, Achim Lother, Krystin Krauel
Summary: This study aimed to assess platelet reactivity in patients with moderate COVID-19 at early stages. Platelet reactivity was evaluated using whole-blood impedance aggregometry and flow cytometry in 20 COVID-19-positive patients, 55 COVID-19-negative patients (as patient controls), and 15 healthy individuals.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2023)
