Review
Cell Biology
Hariharan Subramanian, Viacheslav O. Nikolaev
Summary: The rate of calcium cycling and calcium transient amplitude are critical for efficient heart contraction and relaxation. Altered calcium-handling proteins in heart failure can be restored through the regulation of cAMP/PKA, with AKAPs playing a key role in this process. Targeting AKAPs shows potential in improving defective calcium cycling, and important studies have identified their regulatory roles in cardiac myocyte calcium handling.
Review
Biochemistry & Molecular Biology
Duangnapa Kovanich, Teck Yew Low, Manuela Zaccolo
Summary: cAMP is a second messenger that plays a crucial role in regulating cellular functions. The compartmentalization of cAMP signaling is essential for its specificity, and the formation of dynamic signaling domains is responsible for precise spatiotemporal regulation. Proteomics can be used to identify the components of these domains and define the dynamic landscape of cellular cAMP signaling. Understanding compartmentalized cAMP signaling in physiological and pathological conditions can provide insights into disease mechanisms and guide the development of precision medicine interventions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Elia Mota, Segolene Bompierre, Dahdjim Betolngar, Liliana R. Castro, Pierre Vincent
Summary: This study reveals the functional contribution of PDE2A, PDE4, and PDE10A in regulating cAMP/PKA response to dopamine stimulation in the striatum. PDE10A is found to play a key role in reducing cAMP levels back to baseline and dephosphorylating PKA substrates. Inhibiting PDE10A dampens responsiveness to dopamine, while inhibiting PDE2A or PDE4 strengthens dopamine action.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Roberta Paolillo, Stefania D'Apice, Gabriele Giacomo Schiattarella, Pietro Ameri, Domenica Borzacchiello, Daniele Catalucci, Cristina Chimenti, Lia Crotti, Sebastiano Sciarretta, Daniele Torella, Antonio Feliciello, Cinzia Perrino
Summary: The second messenger cAMP regulates multiple mitochondrial functions through the activation of cAMP/PKA signaling. AKAPs play important roles in regulating cardiac functions. Understanding the roles and regulation of mitochondria-targeted AKAPs can help modulate their functions for clinical purposes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Armando Jesus Perez-Diaz, Beatriz Vazquez-Marin, Jero Vicente-Soler, Francisco Prieto-Ruiz, Teresa Soto, Alejandro Franco, Jose Cansado, Marisa Madrid
Summary: The autophagy pathway in yeast is regulated differently in response to glucose limitation, indicating evolutionary variations in the signaling pathways that govern autophagy during glucose shortage or starvation.
Article
Biochemistry & Molecular Biology
Blair K. A. Willette, Jin-Fan Zhang, Jin Zhang, Nikoleta G. Tsvetanova
Summary: Endosome positioning plays a key role in regulating GPCR signaling and initiating transcriptional responses. Disruption of native endosome positioning inhibits GPCR-dependent transcriptional signaling, highlighting its importance in spatially selective receptor signaling.
NATURE CHEMICAL BIOLOGY
(2023)
Review
Pharmacology & Pharmacy
Kinjal Patel, Nicola J. Smith
Summary: Primary cilia are important for sensing environmental changes in mammalian cells through the Sonic Hedgehog pathway. GPR161 is a significant player in primary cilia and its unique features may affect receptor function and cAMP compartmentalisation. The recent potential pairing of GPR161 and spexin-1 requires further investigation before GPR161 is considered 'deorphanised'. The constitutive activity and unconventional regulation of GPR161 suggest that it may not require an endogenous ligand.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Cell Biology
Darko Maric, Aleksandra Paterek, Marion Delaunay, Irene Perez Lopez, Miroslav Arambasic, Dario Diviani
Summary: The study demonstrates that AKAP2 plays a role in promoting cardioprotection in infarcted hearts, with its knockout leading to increased infarct size and worsened cardiac function. AKAP2 forms a complex with PKA and Src3 in cardiomyocytes, facilitating the activation of ERα and upregulation of genes involved in anti-apoptosis and angiogenesis. Decreased AKAP2 expression results in increased myocardial apoptosis and impaired formation of new blood vessels in infarcted hearts.
Article
Neurosciences
Alaa Emam Ali, Doaa Mokhtar Elsherbiny, Samar S. Azab, Ebtehal El-Demerdash
Summary: The study demonstrated that Amiloride could effectively alleviate neurodegeneration and memory impairment induced by doxorubicin chemotherapy through inhibiting different ion transporters, reducing oxidative damage and cell death, decreasing neuroinflammation and oxidative stress, as well as inhibiting hippocampal apoptosis. This suggests potential new therapeutic applications of Amiloride in hypertensive cancer patients for its neuroprotective effects.
Article
Physiology
Samuel L. Svendsen, Simon Kornvig, Peder Berg, Iben S. Jensen, Isabela B. B. A. de Araujo, Casper K. Larsen, Jens Leipziger, Mads Sorensen
Summary: K+-induced diuresis involves desensitization of the collecting duct to AVP.
Review
Endocrinology & Metabolism
T. John Martin
Summary: Parathyroid hormone (PTH) activates adenylyl cyclase in cells of the osteoblast lineage, indirectly regulating osteoclast formation. PTH1R, shared by PTH and PTHrP, activates adenylyl cyclase with similar potency. PTH1R is translocated from the plasma membrane to the endosome, but the influence of endosomal translocation on PTH1R activity is not yet known.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Caitlin Walker, Yingjie Wang, Cristina Olivieri, V. S. Manu, Jiali Gao, David A. Bernlohr, Davide Calebiro, Susan S. Taylor, Gianluigi Veglia
Summary: Somatic mutations in the PRKACA gene lead to the development of Cushing's syndrome, with the E31V allosteric mutation disrupting communication nodes and nucleotide-substrate binding cooperativity. The loss of binding cooperativity in Cushing's syndrome mutants is proportional to the density of the intramolecular allosteric network, suggesting a common mechanism for this disease.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mateusz Kwiatkowski, Aloysius Wong, Anna Kozakiewicz-Piekarz, Christoph Gehring, Krzysztof Jaworski
Summary: This passage discusses the roles of signaling molecules cAMP and cGMP, as well as Ca2+ in plants, and investigates the function of PDEs in monocot plants, identifying a candidate PDE from Brachypodium distachyon. The research shows that the PDE has a significant hydrolytic activity towards cAMP, which can be further enhanced by Ca2+ and CaM. Additionally, a comprehensive amino acid consensus sequence is constructed to aid in the identification of similar active sites in eukaryotes and prokaryotes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Masashi Kuriyama, Hisaaki Hirose, Toshihiro Masuda, Masachika Shudou, Jan Vincent Arafiles, Miki Imanishi, Masashi Maekawa, Yuji Hara, Shiroh Futaki
Summary: This study discovers that inhibition of macropinocytosis can be achieved through regulating the activity of mechanosensitive channels, offering a new strategy for cancer therapy.
SCIENTIFIC REPORTS
(2022)
Article
Obstetrics & Gynecology
Darja Lavogina, Artjom Stepanjuk, Maire Peters, Kulli Samuel, Sergo Kasvandik, Masuma Khatun, Riikka K. Arffman, Erki Enkvist, Kaido Viht, Sergei Kopanchuk, Freddy Lattekivi, Agne Velthut-Meikas, Asko Uri, Terhi T. Piltonen, Ago Rinken, Andres Salumets
Summary: The study identified elevated ROCK activity in response to combined progesterone and 8-Br-cAMP treatment during in vitro decidualization, leading to increased ROCK2 mRNA and protein levels as well as phosphorylation of its downstream target Cofilin-1. This research highlighted the importance of ROCK as a novel target in decidualization and the need for further investigation, especially in the context of subfertility and infertility related to decidualization.
HUMAN REPRODUCTION
(2021)
Article
Cell Biology
Jean-Paul V. Paluzzi, Amir Saleem Haddad, Laura Sedra, Ian Orchard, Angela B. Lange
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2015)
Article
Neurosciences
Jean-Paul V. Paluzzi, Garima Bhatt, Chang-Hui J. Wang, Meet Zandawala, Angela B. Lange, Ian Orchard
FRONTIERS IN NEUROSCIENCE
(2015)
Article
Endocrinology & Metabolism
David A. Rocco, Jean-Paul V. Paluzzi
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Kinsi Gondalia, Anarn Qudrat, Brigida Bruno, Janet Fleites Medina, Jean-Paul V. Paluzzi
Article
Biochemistry & Molecular Biology
Alireza Oryan, Azizia Wahedi, Jean-Paul V. Paluzzi
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2018)
Article
Biology
Farwa Sajadi, Carmela Curcuruto, Afra Al Dhaheri, Jean-Paul V. Paluzzi
JOURNAL OF EXPERIMENTAL BIOLOGY
(2018)
Article
Multidisciplinary Sciences
Jean-Paul Paluzzi, Mark Vanderveken, Michael J. O'Donnell
Article
Multidisciplinary Sciences
Azizia Wahedi, Jean-Paul Paluzzi
SCIENTIFIC REPORTS
(2018)
Article
Endocrinology & Metabolism
Azizia Wahedi, Gerd Gade, Jean-Paul Paluzzi
FRONTIERS IN ENDOCRINOLOGY
(2019)
Article
Genetics & Heredity
Meet Zandawala, Thomas Nguyen, Marta Balanya Segura, Helena A. D. Johard, Mirjam Amcoff, Christian Wegener, Jean-Paul Paluzzi, Dick R. Nassel
Summary: Insects utilize a neuroendocrine axis involving Crz and CAPA neuropeptides to coordinate responses to metabolic and osmotic stress. Crz inhibits the release of CAPA to restore metabolic and osmotic homeostasis, impacting stress tolerance.
Article
Multidisciplinary Sciences
Andrew A. Walker, Samuel D. Robinson, Jean-Paul Paluzzi, David J. Merritt, Samantha A. Nixon, Christina Schroeder, Jiayi Jin, Mohaddeseh Hedayati Goudarzi, Andrew C. Kotze, Zoltan Dekan, Andy Sombke, Paul F. Alewood, Bryan G. Fry, Marc E. Epstein, Irina Vetter, Glenn F. King
Summary: The study reveals that the limacodid caterpillar, Doratifera vulnerans, produces a complex venom containing 151 proteinaceous toxins, most of which are peptides <10 kDa. Three families of venom peptides seem to be responsible for inducing pain in mammals. The findings suggest that lepidopteran venoms are a potential source of novel bioactive peptides.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Entomology
Nia Keyes-Scott, Aryan Lajevardi, Kyle R. Swade, Mark R. Brown, Jean-Paul Paluzzi, Kevin J. Vogel
Summary: Mosquito reproduction is regulated by hormones acting through membrane-bound receptor proteins. Two receptor proteins, AeCNMaR-1a and AeCNMaR-1b, in Aedes aegypti were identified as orthologs of the fruit fly CNMa receptor. The receptors have different expression patterns and respond to a hormone at different concentrations. Injecting the hormone into mated female mosquitoes resulted in reduced egg-laying.
Article
Multidisciplinary Sciences
Luca Finetti, Jean-Paul Paluzzi, Ian Orchard, Angela B. Lange
Summary: In insects, the biogenic amines octopamine (OA) and tyramine (TA) and their receptors play important roles in controlling various physiological and behavioral processes. This study identified and characterized the OA and TA receptors in Aedes aegypti, the mosquito species known for transmitting dengue and yellow fever. The receptors were found to be expressed in all developmental stages, with the highest abundance observed in adults. Furthermore, the expression patterns of these receptors were influenced by a blood meal, implicating their involvement in feeding-related physiological processes.
Article
Cell Biology
David A. Rocco, Doo Hyun Kim, Jean-Paul V. Paluzzi
CELL AND TISSUE RESEARCH
(2017)