Review
Immunology
Jason Cheung, Beata Zahorowska, Michael Suranyi, Jeffrey K. W. Wong, Jason Diep, Stephen T. T. Spicer, Nirupama D. D. Verma, Suzanne J. Hodgkinson, Bruce M. M. Hall
Summary: The immune response to an allograft can activate lymphocytes that cause rejection. The activation of T regulatory cells can reduce allograft rejection and induce immune tolerance. Activated T regulatory cells can be distinguished by various markers. A more detailed characterization of these cells may help reduce non-specific immunosuppression.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Laurene Pousse, Koorosh Korfi, Bruno C. C. Medeiros, Marco Berrera, Nadine Kumpesa, Jan Eckmann, Idil Karakoc Hutter, Vera Griesser, Vaios Karanikas, Christian Klein, Maria Amann
Summary: By analyzing the bone marrow and/or blood samples of 37 AML patients and healthy donors, it was found that the composition of the bone marrow is strongly correlated with that of the blood. CD25 expressing AML cells were found to be enriched in FLT3-ITD mutation patients and patients treated with a hypomethylating agent in combination with venetoclax. CD25 Mab antibody can specifically kill CD25+ AML cells and regulatory T cells, providing a potential treatment for leukemia.
FRONTIERS IN ONCOLOGY
(2023)
Article
Immunology
Jessica G. Lee, Kathleen E. Jaeger, Yoichi Seki, Yi Wei Lim, Christina Cunha, Aleksandra Vuchkovska, Alexander J. Nelson, Anya Nikolai, Dan Kim, Michael Nishimura, Katherine L. Knight, Paula White, Makio Iwashima
Summary: The study reveals that a subset of CD14(+) monocytes can generate regulatory Foxp3(+) T-bet(+) T cells from umbilical cord blood, which suppress T-cell proliferation and ameliorate graft-versus-host disease. Additionally, adult peripheral blood monocytes are capable of inducing Foxp3(+) T cells, but their induction is inhibited by lymphoid cells from adult peripheral blood in neonates. This suggests a novel immunoregulatory role of monocytes in generating regulatory T cells with implications for both neonates and adults.
Article
Surgery
Katsuyoshi Shimozawa, Laura Contreras-Ruiz, Sofia Sousa, Ruan Zhang, Urvashi Bhatia, Kerry C. Crisalli, Lisa L. Brennan, Laurence A. Turka, James F. Markmann, Eva C. Guinan
Summary: The study compared the suitability of blood cells from pre- and post-transplant patients with healthy controls for Treg manufacturing, finding that Treg expansion capacity was preserved despite certain limitations. Leukapheresis post-transplant and the use of splenocytes as substitutes for PBMCs were developed to improve Treg yield. The research also demonstrated how characterizing cellular input populations can inform clinical trial design and Treg manufacturing requirements.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Article
Cell Biology
Agnese Fiori, Stefanie Uhlig, Harald Kluter, Karen Bieback
Summary: Mesenchymal stromal cells (MSC) have immunomodulatory effects on various immune cells. In this study, human adipose tissue-derived MSC (ASC) were found to inhibit CD4 + T cell proliferation, induce Treg cells, and modulate cytokine secretion, suggesting a potential role in immune regulation. These findings highlight the complex interplay between ASC and CD4 + T cells.
Article
Chemistry, Multidisciplinary
Varvara G. Blinova, Natalia S. Novachly, Sofya N. Gippius, Abdullah Hilal, Yulia A. Gladilina, Daria D. Eliseeva, Dmitry D. Zhdanov
Summary: The study tracked the differentiation and maturation of Tregs CD4(+)CD25(+)FoxP3(+)CD127(low) cells over 7 days of cultivation under ex vivo conditions, showing that expanded eTregs have a Treg-specific phenotype and suppressive activity. As expansion progressed, upregulation of the FoxP3 gene was observed and associated with gradual demethylation in the T cell-specific demethylation region.
APPLIED SCIENCES-BASEL
(2021)
Article
Immunology
Nicolas Sailliet, Hoa-Le Mai, Amandine Dupuy, Gaelle Tilly, Cynthia Fourgeux, Martin Braud, Magali Giral, Jean-Michel Robert, Nicolas Degauque, Richard Danger, Jeremie Poschmann, Sophie Brouard
Summary: In this study, single cell transcriptomics were used to characterize in vitro induced GZMB+ regulatory B cells (Bregs), showing differential gene expression involved in proliferation, apoptosis, metabolism, and antigen presentation. Compared to total B cells, Bregs exhibited strong inhibition of T cell genes associated with proliferation, activation, inflammation, and apoptosis. Lymphotoxin alpha (LTA) was identified as a new and potent Breg ligand implicated in Breg suppressive properties.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Nicolas Krause, Joerg Mengwasser, Elpida Phithak, Francisca Beato, Marc Appis, Edgar Louis Milford, Johan Pratschke, Igor Sauer, Anja Kuehl, Arndt Vogel, Michael Goodyear, Linda Hammerich, Frank Tacke, Johanna Faith Haas, Tobias Mueller, Nalan Utku
Summary: IR1 cells are a subset of T regulatory cells that have immune suppressive functions and can inhibit proliferation in mixed lymphocyte reactions. They deliver regulatory signals by binding to their ligand, HLA-DR alpha 2, and have the potential to modulate immune dysregulation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Linda M. Lee, Hong Zhang, Karim Lee, Horace Liang, Alexander Merleev, Flavio Vincenti, Emanual Maverakis, Angus W. Thomson, Qizhi Tang
Summary: In this study comparing arTregs expanded ex vivo using different types of antigen-presenting cells, it was found that sDCs stimulated Tregs to expand in much larger numbers. Additionally, sDC-generated arTregs expressed higher levels of CD80, CD86, and T cell-attracting chemokines, indicating their superior expansion-inducing capacity.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Stephane Fattori, Aude Le Roy, Jemila Houacine, Lucie Robert, Riad Abes, Laurent Gorvel, Samuel Granjeaud, Marie-Sarah Rouviere, Amira Ben Amara, Nicolas Boucherit, Carole Tarpin, Jihane Pakradouni, Emmanuelle Charafe-Jauffret, Gilles Houvenaeghel, Eric Lambaudie, Francois Bertucci, Philippe Rochigneux, Anthony Goncalves, Arnaud Foussat, Anne-Sophie Chretien, Daniel Olive
Summary: The role of regulatory T cells (Tregs) in triple-negative breast cancer (TNBC) remains controversial. This study investigates the tumor microenvironment in TNBC and finds an imbalance between suppressive Tregs and effector T cells. The selective surface marker CD25 may be a promising target for Treg depletion therapy. Furthermore, the combination of anti-CD25 antibodies and PD-1 blockade shows potential for improving antitumor immunity in TNBC.
Article
Neurosciences
Alireza Faridar, Matthew Vasquez, Aaron D. Thome, Zheng Yin, Hui Xuan, Jing Hong Wang, Shixiang Wen, Xuping Li, Jason R. Thonhoff, Weihua Zhao, Hong Zhao, David R. Beers, Stephen T. C. Wong, Joseph C. Masdeu, Stanley H. Appel
Summary: This study demonstrates that ex vivo expanded Tregs can suppress neuroinflammation and alleviate amyloid pathology in a preclinical mouse model of Alzheimer's disease. These findings provide potential therapeutic strategies for Treg cell therapy in AD.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Marit J. van Elsas, Johan M. S. van der Schoot, Alexander Bartels, Kas Steuten, Duco van Dalen, Zacharias Wijfjes, Carl G. Figdor, Thorbald van Hall, Sjoerd H. van der Burg, Martijn Verdoes, Ferenc A. Scheeren
Summary: Regulatory T cells play a crucial role in immune suppression and pose challenges in cancer therapy. By optimizing the Fc domain of an antibody, efficient depletion of tumor-resident regulatory T cells can be achieved. Using a genome engineering strategy, a stable cell line producing optimized antibodies was generated, leading to effective depletion of tumor-resident regulatory T cells and enhanced tumor eradication when combined with other antibodies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Beatriz F. F. Corte-Real, Rebeca Arroyo Hornero, Aleksandra Dyczko, Ibrahim Hamad, Markus Kleinewietfeld
Summary: CSF2RB (CD131) may serve as a potential biomarker for autoimmune diseases in Tregs, with its overexpression potentially being linked to disease development.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Andrew E. Wight, Jessica M. Sido, Sandrine Degryse, Lin Ao, Hidetoshi Nakagawa, Yiguo Qiu(Vivian), Xianli Shen, Oba Oseghali, Hye-Jung Kim, Harvey Cantor
Summary: Regulatory T cells (Treg) expressing interleukin 23 receptor (IL23R) promote suppressive activity, hindering antitumor immunity. Disrupting IL23R enhances Treg responsiveness to IL12, resulting in increased production of γ-interferon and recruitment of CD8 T cells that inhibit tumor growth. Combined targeting of destabilization pathways in Treg can lead to robust and synergistic antitumor responses.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Nirupama D. Verma, Andrew D. Lam, Christopher Chiu, Giang T. Tran, Bruce M. Hall, Suzanne J. Hodgkinson
Summary: The study found significant shifts in CD4(+) T cell subpopulations in multiple sclerosis (MS) patients, with reduced resting Treg, increased activated Treg, and increased effector CD4(+) cells. Additionally, some MS patients showed reduced CCR6(+)Th17-like Treg, which may contribute to the activity of MS.
SCIENTIFIC REPORTS
(2021)