Article
Oncology
Vita Golubovskaya, Hua Zhou, Feng Li, Michael Valentine, Jinying Sun, Robert Berahovich, Shirley Xu, Milton Quintanilla, Man Cheong Ma, John Sienkiewicz, Yanwei Huang, Lijun Wu
Summary: CAR-T cell therapy, specifically targeting CD19, has been approved by the FDA for leukemia and lymphoma treatment. This study introduces novel CD37 and CD37-CD19 CAR-T cells, demonstrating their specific targeting abilities and laying the foundation for future clinical studies.
Article
Multidisciplinary Sciences
Senichiro Yanagawa, Hiroyuki Tahara, Takayuki Shirouzu, Shintaro Kawai, Yuka Tanaka, Kentaro Ide, Shuji Akimoto, Hideki Ohdan
Summary: This study aimed to establish a humanized mouse model for analyzing the mechanism of anti-allogeneic human B cell tolerance induction. By injecting NSG mice with PBMCs mixed with different HLA antigenic combinations, sufficient human total-IgG and anti-HLA antibodies were produced, but allospecific anti-HLA antibody production was suppressed.
Review
Immunology
Min Hu, Natasha M. Rogers, Jennifer Li, Geoff Y. Zhang, Yuan Min Wang, Karli Shaw, Philip J. O'Connell, Stephen Alexander
Summary: Tregs play a crucial role in kidney transplantation by limiting immune activation and potentially reducing the need for immunosuppression. Studies have shown their importance in improving allo-specific Treg function in both animal and human models.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Angela Wahl, Wenbo Yao, Baolin Liao, Morgan Chateau, Cara Richardson, Lijun Ling, Adrienne Franks, Krithika Senthil, Genevieve Doyon, Fengling Li, Josh Frost, Christopher B. Whitehurst, Joseph S. Pagano, Craig A. Fletcher, M. Andrea Azcarate-Peril, Michael G. Hudgens, Allison R. Rogala, Joseph D. Tucker, Ian McGowan, R. Balfour Sartor, J. Victor Garcia
Summary: Germ-free mice are commonly used in studying the role of the microbiome in health and disease, but they are not suitable for studying human-specific pathogens. In this study, a germ-free mouse model systemically reconstituted with human immune cells was developed and used to evaluate the role of the resident microbiome in the acquisition, replication, and pathogenesis of Epstein-Barr virus (EBV) and human immunodeficiency virus (HIV). The results showed that the resident microbiome enhances the establishment of EBV infection, EBV-induced tumorigenesis, mucosal HIV acquisition, and replication.
NATURE BIOTECHNOLOGY
(2023)
Article
Immunology
Yixin Wang, Lei Wang, Cong Fu, Xue Wang, Siyao Zuo, Chang Shu, Yanhong Shan, Jin He, Qi Zhou, Wei Li, Yong-Guang Yang, Zheng Hu, Shucheng Hua
Summary: This study utilized a humanized mouse model with human lung tissue xenografts to investigate the immunopathogenesis and therapeutic interventions of viral respiratory diseases. The findings highlight the importance of tissue-resident memory T cells and viral Ag-specific T cells in the immune response against respiratory viruses.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Food Science & Technology
Maria-Carmen Lopez de las Hazas, Andrea del Saz-Lara, Lidia Cedo, Maria Carmen Crespo, Joao Tome-Carneiro, Luis A. Chapado, Alba Macia, Francesco Visioli, Joan C. Escola-Gil, Alberto Davalos
Summary: HT supplementation in humanized mice resulted in systemic dyslipidemia, impaired glucose metabolism, increased body weight, and accumulation of triacylglycerols in different tissues. The expression of genes related to lipid metabolism, such as Pltp or Lpl, was also modulated by HT. Further studies are needed to fully understand the potential effects of HT consumption.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Medicine, Research & Experimental
Mengyan Li, Scott J. Garforth, Kaitlyn E. O'Connor, Hang Su, Danica M. Lee, Alev Celikgil, Rodolfo J. Chaparro, Ronald D. Seidel, R. Brad Jones, Ravit Arav-Boger, Steven C. Almo, Harris Goldstein
Summary: In this study, synTacs were used to expand highly functional HIV- and CMV-specific CD8(+) T cells, which showed potent antiviral activities in vivo. The results suggest that synTac-based approaches could be a promising therapeutic strategy for HIV cure and treatment of CMV and other viral infections.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Oncology
Christopher A. Pennell, Heather Campbell, Meghan D. Storlie, Sara Bolivar-Wagers, Mark J. Osborn, Yosef Refaeli, Michael Jensen, Sophie Viaud, Travis S. Young, Bruce R. Blazar
Summary: Current FDA-approved CAR-T cell therapies for B-cell malignancies are effective but can increase morbidity and mortality. This study compared the performances of regulatable and constitutively active CAR-T cells, and found that regulatable CAR-T cells have the potential for improved efficacy and reduced toxicity.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Biochemistry & Molecular Biology
Marija Drakul, Sergej Tomic, Marina Bekic, Dusan Mihajlovic, Milos Vasiljevic, Sara Rakocevic, Jelena Dokic, Nikola Popovic, Dejan Bokonjic, Miodrag Colic
Summary: Sitagliptin has an effect on the differentiation and maturation of human dendritic cells, with decreased expression of some immune activity molecules and increased expression of some tolerogenic molecules. Sitagliptin-treated cells have lower ability to stimulate T-cells and suppress certain T-cell responses while enhancing immunoregulatory responses. This effect may be achieved through the inhibition of NF-kB and p38MAPK expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Gastroenterology & Hepatology
Pajau Vangay, Tonya Ward, Sarah Lucas, Lalit K. Beura, Dominique Sabas, Max Abramson, Lisa Till, Susan L. Hoops, Purna Kashyap, Ryan C. Hunter, David Masopust, Dan Knights
Summary: In this study, the differential effects of human gut microbiota from the United States and rural Thailand on the murine gut mucosa and immune system were assessed. The results suggest that Western-associated human gut microbes contribute to a pro-inflammatory immune response.
Article
Allergy
Erika Mendez-Enriquez, Maya Salomonsson, Jens Eriksson, Christer Janson, Andrei Malinovschi, Mikael E. Sellin, Jenny Hallgren
Summary: It has been found that mast cell progenitors can be activated by IgE cross-linking, which may contribute to the development of acute allergic airway inflammation.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Cell Biology
Nada S. Alakhras, Jaeho Shin, Scott A. Smith, Anthony L. Sinn, Wenwu Zhang, Gyoyeon Hwang, Jenna Sjoerdsma, Emily K. Bromley, Karen E. Pollok, Basar Bilgicer, Mark H. Kaplan
Summary: Peanut-induced allergy is a type I hypersensitivity reaction mediated by IgE, which can range from local edema to life-threatening anaphylaxis. There are currently few preventive therapies for this type of allergy, but a covalent heterobivalent inhibitor (cHBI) has shown promise in both in vitro and in vivo models. In this study, a humanized mouse model expressing human mast cells was developed and used to demonstrate that cHBI can prevent allergic reactions, including fatal anaphylaxis, when administered before or shortly after allergen exposure.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Article
Multidisciplinary Sciences
Caroline Lamarche, Kirsten Ward-Hartstonge, Tian Mi, David T. S. Lin, Qing Huang, Andrew Brown, Karlie Edwards, Gherman E. Novakovsky, Christopher N. Qi, Michael S. Kobor, Caitlin C. Zebley, Evan W. Weber, Crystal L. Mackall, Megan K. Levings
Summary: Regulatory T cells (Tregs) are susceptible to exhaustion, which can limit their therapeutic effect. A comprehensive investigation on exhaustion in Tregs reveals key similarities and differences with exhausted conventional T cells. This finding has important implications for the design of CAR Treg adoptive immunotherapy strategies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Immunology
Rui Zheng, Yuankun Chen, Yiting Zhang, Sixin Liang, Xiaojuan Zhao, Yiyi Wang, Pengju Wang, Ruotong Meng, Angang Yang, Bo Yan
Summary: Our study explores the effect of low-affinity CARs using humanized scFvs on the function of CAR-T cells. We find that moderately reducing the affinity of CARs can maintain anti-tumor efficacy and improve the safety of CAR therapy both in vitro and in vivo. In addition, T cells expressing the VL domain only antibody show long-lasting tumor elimination capability and lower cytokine levels.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Christopher S. Garris, Jeffrey L. Wong, Jeffrey Ravetch, David A. Knorr
Summary: This study demonstrates that CD40-expressing dendritic cells in the bladder tumor microenvironment can be targeted through intravesical delivery of CD40 agonist antibodies, leading to potent antitumor immune responses and reduction of exhausted CD8(+) T cells. These findings suggest a promising approach for the treatment of high-risk non-muscle-invasive bladder cancer.
SCIENCE TRANSLATIONAL MEDICINE
(2021)